Objective: To observe the clinical effect of auricular point sticking therapy for cardiac syndrome X (CSX) in women. Methods: A total of 64 patients were randomized into two groups by the random number table, with 32 cases in each group. Patients in the control group received conventional treatment for angina, while patients in the treatment group received auricular point sticking therapy on the basis of conventional treatment. After 8 weeks of treatment, the levels of serum estradiol (E2), nitric oxide (NO) and endothelium-1 (ET-1) were compared to evaluate the therapeutic effect. Results: After 8 weeks of treatment, the total effective rate and markedly effective rate in the treatment group were significantly higher than those in the control group, and the between-group comparisons showed statistical significance (both P<0.05). After treatment, the scores of chest pain and tightness in the control group dropped significantly, and the intra-group comparisons showed statistical significance (both P<0.05); the scores of chest pain and tightness, palpitations, weakness and shortness of breath in the treatment group dropped significantly, and the intra-group comparisons showed statistical significance (all P<0.05); there were significant inter-group differences in the scores of chest pain and tightness. After treatment, the intra-group comparisons of E2, NO and ET-1 contents in the control group showed no statistical significance (all P>0.05), while the E2, NO and ET-1 contents in the treatment group changed significantly after treatment and were significantly different from those in the control group (all P<0.05). Conclusion: Auricular point sticking on the basis of conventional treatment was effective for CSX in women, and is worth clinical application.

Objective: To investigate the changes of matrine concentration in rat local skin with time after transdermal administration of matrine transfersomes, and to evaluate the transdermal delivery properties. Methods: The matrine transfersomes were applied non-occlusively onto rat skin in vivo with abdominal hair removal, and the concentration of drugs in microdialysate of skin was detected by microdialysis and reverse phase high performance liquid chromatography. Furthermore, the concentration-time curves of matrine in microdialysates of skin were compared among marine transfersomes, liposomes, and deoxysodium cholate solution. Results: After the transfersomes were given to rats, the maximum peak time (tmax) of matrine skin concentration appeared at (4.200 ± 0.447) h. The maximum skin concentration (Cmax) was (0.927 ± 0.251) μg/mL and area under the curve (AUC0-8) was (5.033 ± 1.526) μg·h·mL-1, which were much higher than those of the liposomes and the solution (containing 0.8% sodium deoxycholate, P < 0.05), while tmax shortened much more than that of them. Conclusion: In vivo skin microdialysis could be used to assess the transdermal delivery properties of matrine transfersomes. And matrine transfersomes have the good transdermal permeability and efficacy.

Objective To explore the feasible of diagnosing ischemic cerebrovascular diseases by detecting central retina artery sclerosis(CRA) with color Doppler ultrasonography.Methods210 patients with ischemic cerebrovascular disease(420 eyes) and 80 healthy volunteers(160 eyes) were examined by color Doppler flow imaging(CDFI) to detect double sides blood frequency spectrum of CRA and hemodynamic parameters of CRA including peak systolic velocity(PSV),end of diastolic velocity(EDV)and resistant index(RI).ResultsThe blood frequency spectrum of CRA showed three apices and two vales or slanting triangle in healthy volunteers.But in patients with ischemic cerebrovascular disease,the blood frequency spectrum changed,and PSV and EDV were slower and RI increased compared with those in the healthy volunteers(PP<0.05～0.001).ConclusionIn patients with ischemic cerebrovascular disease,the blood flow of CRA is slower and RI increases compared with health individuals.The color Doppler ultrasonography can used for diagnosis of CRA sclerosis.

Abstract: Objective　To analyze the treatment outcomes of elderly patients with pulmonary tuberculosis in Chongqing, so as to provide reference for the prevention and control strategies of the epidemic of elderly pulmonary tuberculosis. Methods　The data of tuberculosis cases aged ≥65 years in Chongqing from 2015 to 2020 were collected from the National Health Insurance Information Project Disease Prevention and Control Information system. Descriptive statistical methods were used to analyze the data. Results The registration rates of elderly active pulmonary tuberculosis patients and etiological positive patients were 110.95/10-5and 32.25/10-5 in 2015 and 84.06/10-5 and 57.29/10-5 in 2020. The annual decline rate of active tuberculosis registration was 5.40%, and the annual increase rate of pathogenic-positive tuberculosis registration was 12.18%. The registration rates of active tuberculosis patients and etiological positive patients in the whole population were 70.75/10-5 and 17.63/10-5 in 2015 and 50.34/10-5 and 29.14/10-5 in 2020. The annual decline rate of active tuberculosis registration was 6.58%, and the annual increase rate of pathogenic-positive tuberculosis registration was 10.57%. From 2015 to 2020, a total of 25 931 cases of elderly pulmonary tuberculosis were registered, of which 21 374 (82.43%) cases were successfully treated and 4 010 (15.80%) cases had unfavorable outcomes. The proportion of cured and death patients showed an increasing trend year by year (χ2trend=313.853, 100.502, P<0.01). From 2015 to 2020, the average annual successful treatment rate of elderly pulmonary tuberculosis in the whole city was 82.43%, with the lowest rate in southeast Chongqing (74.23%), followed by urban areas (81.99%). The success rate of elderly pulmonary tuberculosis treatment in the whole city, west Chongqing, northeast Chongqing and southeast Chongqing showed a downward trend year by year (χ2trend=230.199, 35.278, 108.076, 112.130, all P<0.01), with annual decline rates of 2.77%, 2.26%, 3.0% and 4.12%, respectively. Among the registered elderly patients, female, 65-<75 years old, Han nationality, newly diagnosed, no complications, and negative for etiology (χ2=15.234, 255.910, 146.842, 179.998, 25.575, 131.170, P<0.01) had higher success treatment rates. Conclusions The prevalence of pulmonary tuberculosis in the elderly population in Chongqing City is declining, but the positive registration rate of etiology is increasing annually, and the success rate of treatment is decreasing. Therefore, it is necessary to strengthen the systematic management, publicity and education of elderly patients (especially those in southeast Chongqing, male, positive patients and severe patients) to effectively control the epidemic of tuberculosis in the elderly.

Objective To evaluate the laparoscopic Palomo procedure in the management of varicocele in adolescent by assessment of the postoperative testicular growth and the changes of the biggest vessel diameter of varix. Methods From July 1999 to March 2004, laparoscopic high mass - ligation of testicular vessels according to Palomo procedure was performed on 31 boys who all presented with left varicocele Before and after operation at 1 month,3 and 6 months, the biggest vessel diameter of varicocele was measured by ultrasonography, testicular blood supply was evaluated by color Doppler flow imaging(CDFI) ,and fastis was recorded in length, width and thickness by ultrasonographic measurement, which volume was calculated by the formula V =?/6 ? length ? width ? thickness. Also the ratio of left to right testicular volume was calculated, and the left testicle was thought atrophy if the ratio was below 75% The biggest vessel diameter of varix, testicular blood supply, and the postoperative testicular increasing volume were compared before and after operation. Results The biggest vessel diameter of varicocele significantly reduced 1 month after operation(P

Adult ; Antiviral Agents ; therapeutic use ; Colonic Neoplasms ; diagnosis ; etiology ; therapy ; Cyclosporine ; adverse effects ; Esophageal Neoplasms ; diagnosis ; etiology ; therapy ; Female ; Ganciclovir ; therapeutic use ; Humans ; Immunosuppression ; adverse effects ; Immunosuppressive Agents ; adverse effects ; Liver Neoplasms ; diagnosis ; etiology ; therapy ; Liver Transplantation ; adverse effects ; Lymphoproliferative Disorders ; diagnosis ; etiology ; therapy ; Middle Aged ; Pharyngeal Neoplasms ; diagnosis ; etiology ; therapy ; Survival Analysis ; Tacrolimus ; adverse effects ; Time Factors

According to teaching practice of "microbial engineering", teaching reforms, such as conformity and optimization of the curriculum system, improvement of teaching methods and the construction of multilevels experimental teaching system, are investigated in this paper, in order to improve the teaching quality and enhance the overall quality and the abilities of operation and innovation of students.

Child, Preschool ; Female ; Humans ; Male ; Shock, Septic ; etiology ; microbiology ; Streptococcal Infections ; complications ; pathology

Objective To investigate the changes of oxidized low density lipoprotein(oxLDL) and C-reactive protein(CRP) and their correlation in patients with acute coronary syndrome(ACS),and explore their roles in the pathogenesis of ACS. Methods The plasma levels of oxLDL and CRP were measured in 95 patients with ACS and 66 control subjects by ELISA. Results oxLDL and CRP levels were significantly higher in patients with ACS than those in control group(P

Background Econazole nitrate is not effective as an antifungal eyedrop because of its poor intraocular permeability,therefore changing the formulation of econazole nitrate to improve its intraocular permeability become a critical point in the treatment of intraocular fungal infection. Objective The present study was to observe the penetration of 0.5％ econazole nitrate nanoparticles in the corneas and aqueous humors following its topicaladministration. Methods Econazole nitrate nanoparticles were prepared by quasi-emulsion solvent diffusion.Characteristics and size of nanoparticles were examined with transmission electron microscope and laser scatteringmethod,respectively.Econazole nitrate nanoparticles drops (0.5％ )was topically administered in 27 New Zealandwhite rabbits bilaterally,and aqueous humor and corneas were obtained after the application of the eye drops for 5,15,30,45,60,90,120,180,240 minutes respectively to detect the concentration of econazole nitrate with highperformance liquid chromatography (HPLC). The pharmacokinetic parameters were calculated with 3 p97pharmacokinetic computer software.The use of the animals followed the Regulation for the Administration of AffairsConcerning Experimental Animals by State Science and Technology Commission. Results The diameter of thenanoparticles was 50 nm with the round shape and encapsulation efficiency was 96.0％.Econazole nitrate nanoparticlesat the concentration of 0.5％ could be rapidly separated with other elements by HPLC with a lowest quantitativeconcentration of 0.1 mg/L.The mean recovery rates of econazole nitrate nanoparticles were 98.09％ in cornea and 99.66％ in aqueous humor,respectively after topical administration.The peak levels of econazole nitrate nanoparticles in cornea and aqueous humor were achieved at 5 minutes after application ( cornea:40.620 μg/g± 7.756 μg/g;aqueous humor:0.504 mg/L±0.153 mg/L),and its half-life( t1/2 )in cornea and aqueous humor was 23.5 minutes and 18.6 minutes,respectively. Conclusions Econazole nitrate nanoparticles at 0.5％ concentration can remain a feasible bioavailability in ocular tissue and therapeutic level in cornea and aqueous humor.