1. Effect of heat stress on expression of small G protein RhoB in rat livers and human prostate cancer cell line PC-3 cells
Academic Journal of Second Military Medical University 2010;28(4):381-384
Objective: To investigate the changes of the small G protein RhoB expression in scalded rat livers and heat-stressed human prostate cancer cell line PC-3, so as to discuss the influence of heat stress on expression of RhoB in vitro and in vivo. Methods: Third degree burns of 30% total body surface area (TBSA) model was established with SD rats on the back. The expression of RhoB mRNA and protein in the liver was determined by RT-PCR and Western blot 2 h, 4 h, 8 h and 16 h (n=6) after scalding; the liver tissues of normal rats were taken as control (n=6). PC-3 cells were allowed to recover for 0.5 h, 1 h, 2 h, 4 h and 8 h in a cellular heat stress model and the expression of RhoB in mRNA and protein were determined by RT-PCR and Western blot, respectively; untreated PC-3 cells were taken as control. Results: The expression of RhoB mRNA in the livers peaked 4 h after scalding, being about 3.2 folds that of the control group (P<0.01); the expression began to decline 8 h after scalding. The expression of RhoB protein peaked 8 h after scalding, significantly higher than that of the control (P<0.01). RhoB mRNA level began to increase 2 h after heat stress treatment and peaked at 4 h, being about 2. 8 folds that of the control (P<0.01). The expression of RhoB protein reached its maximum at 8 h after heat stress treatment (P<0.01). Conclusion: Heat stress can up-regulate the expression of RhoB mRNA and protein in vivo and in vitro.
2.Determination of Chromium(Ⅵ)in Dyeing Wastewater by Flow Injection On-line Separation and Preconcentration with Inductively Coupled Plasma-Atomic Emission Spectrometry
Dan LU ; Ya-Ping LIU ;
Journal of Environment and Health 2007;0(09):-
Objective To establish a new method for the determination of chromium (Ⅵ) in printing and dyeing wastewater. Methods A combined technique was employed for the determination of chromium (Ⅵ) by flow injection on-line separation and preconcentration on a micro column packed with alumina(acidic form) and inductively coupled plasma-atomic emission spectrometry (ICP-AES).The effect of acidity,concentration of eluent and coexistence ion on separation and enrichment was detected.Results The detection limit was 0.72 ?g/L.The LOQ was 2.38 ?g/L.RSDs were 1.45%~2.12%.The recovery rates were 96.0%-104.0%.The concentration enhancement of chromium (Ⅵ) was 10 times for 45 s sample loading.Analytic frequency was 33 samples one hour. Conclusion This method is sensitive,rapid and applicable to the determination of chromium (Ⅵ) in dyeing wastewater.
3.Study on Dosage Application of Herbs in Class Formulas of KaixinSan and Their Actions on Depression
Ping LIU ; Jinliang WANG ; Ya WANG
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(05):-
Objective: To study the dosage application of herbs in class formulas of KaixinSan recorded in Prescriptions of the Bureau of Taiping People's Welfare Pharmacy (Taiping Huimin Hejiju Fang), Essential Recipes for Emergent Use Worth A Thousand Gold (Beiji Qianjin Yao Fang) and Records of Approved Cases of Ancient and Recent Times (Gujin Luyan) and their actions on depression. Method: According to the dosage proportion of different herbs in the three formulas in the three books, two methods were adopted in terms of decocting different herbs separately and mixing them thereafter(group of decocting separately) as well as extracting after decocted all the herbs together (group of decocting together). Classical anti-depression mice models--forced swimming and tail suspending experiments were observed. The data of immovability time of mice were analyzed by statistics. Result: All the class formulas of KaixinSan which have the same medication but different proportion in the three medical books can reduce the immovability time of mice in forced swimming and tail suspending experiments. But the action of KaixinSan recorded in Prescriptions of the Bureau of Taiping People's Welfare Pharmacy was prominent, and the action of the group of decocting together was superior to that of separated decocting. There was significant difference between the group of decocting together in low dosage and the controlled one (P
5.Preliminary study on anti-fatigue effects of enzymatic hydrolyzed animal protein extracted from Pinctada martensii
Chaohua ZHANG ; Ya LIU ; Ping YANG ; Jing ZHANG ; Hongwu JI
Chinese Journal of Marine Drugs 1994;0(04):-
Objective To study the anti-fatigue effects of enzymatic hydrolyzed protein from Pinctada martensii.Methods Swimming time,contents of liver glycogen and serum urea nitrogen after swimming were determined.Results Three dosages of EPA were all able to prolong swimming time and decrease liver glycogen consumption and serum urea nitrogen content after exhausted swimming in various degree.Conclusion The EAP has evident anti-fatigue effects.
6.The practice and exploration of the reform of cultivating master degree students of clinical medicine promoted by the synergy of medical education
Ping ZHANG ; Zhiyu YU ; Baorong CHI ; Dedong LIANG ; Ya LIU
Chinese Journal of Medical Education Research 2015;(9):865-867,868
Our university actively carried out the reform and innovation of the training mode of clinical medicine postgraduates . We implemented the standardized training of resident physicians throughout postgraduate training process , and carried out the comprehensive reform of the training mode of cultivating master degree students of clinical medicine and the standard training of resident doctors. Meanwhile, we trained the master degree students with the synergy of medical education and strengthened the cultivation of students' clinical practice ability, to cultivate qualified doctors for the country, and to improve the quality of postgraduate training.
7.Primary IgA nephropathy in 46 children: association of clinical and pathological findings with prognosis.
Ya-ping WANG ; Ai-min LIU ; Yu-wen DAI
Chinese Journal of Pediatrics 2005;43(11):866-867
Adolescent
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Child
;
Female
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Glomerulonephritis, IGA
;
diagnosis
;
pathology
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Humans
;
Male
;
Prognosis
9.Advances in research on the association between single nucleotide polymorphisms of the DNA repair genes and resistance to platinum-based chemotherapy.
Jia WEI ; Bao-rui LIU ; Ya-ping WANG
Chinese Journal of Oncology 2006;28(3):161-163
DNA Repair
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genetics
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DNA-Binding Proteins
;
genetics
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Drug Resistance, Multiple
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Drug Resistance, Neoplasm
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Endonucleases
;
genetics
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Humans
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Neoplasms
;
drug therapy
;
genetics
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Nuclear Proteins
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genetics
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Organoplatinum Compounds
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therapeutic use
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Platinum Compounds
;
therapeutic use
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Polymorphism, Single Nucleotide
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Transcription Factors
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genetics
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X-ray Repair Cross Complementing Protein 1
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Xeroderma Pigmentosum Group D Protein
;
genetics
10.Framework analysis method in qualitative research.
Xing LIAO ; Jian-ping LIU ; Nicola ROBISON ; Ya-ming XIE
Chinese Journal of Integrated Traditional and Western Medicine 2014;34(5):622-626
In recent years a number of qualitative research methods have gained popularity within the health care arena. Despite this popularity, different qualitative analysis methods pose many challenges to most researchers. The present paper responds to the needs expressed by recent Chinese medicine researches. The present paper is mainly focused on the concepts, nature, application of framework analysis, especially on how to use it, in such a way to assist the newcomer of Chinese medicine researchers to engage with the methodology.
Humans
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Medicine, Chinese Traditional
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Qualitative Research
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Research
;
Research Design