1. Preparation and quality evaluation of self-emulsifying drug delivery system of total saponins of Sanguisorba officinalis
Chinese Traditional and Herbal Drugs 2018;49(6):1317-1323
Objective: To optimize the formulation of the self-emulsifying drug delivery system of total saponins of Sanguisorba officinalis and evaluate its characteristics. Methods: The formulation and its proportion of the self-emulsifying drug delivery system of total saponins of S. officinalis were optimized based on the solubility tests, formula compatibility, microemulsion area in the ternary phase diagram and D-optimal mixing experiment design. The dosage of oil phase, surfactant and co-surfactant were investigated by drug loading, particle size, and polydispersity index. The appearance, particle size, polydispersity index, Zeta potential, and in vitro release of preparation were finally evaluated. Results: The ratio of oil phase, surfactant, and co-surfactant was 0.25: 0.45:0.30. The drug loading was 23.93 mg/g, the average particle size was (207.92 ± 2.13) nm and the zeta potential was (38.84 ± 0.18) mV. The release of SEDDS was superior to the bulk drug apparently. Conclusion: The self-emulsifying drug delivery system of total saponins of S. officinalis was established. The technology was feasible and the quality was stable.
2.Primary visceral myopathy: report of a case.
Yu-hua ZHOU ; Ya-bin ZOU ; Miao LI ; Xun SUN
Chinese Journal of Pathology 2012;41(8):568-569
3.Chemopreventive Effect of Salvianolic Acid B on Oral Carcinogenesis
ya, YANG ; de-rong, ZOU ; zeng-tong, ZHOU
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(10):-
Objective To investigate the chemopreventive potential of salvianolic acid B against 7,12-dimethylbenz(a)anthracene(DMBA)-induced hamster buccal pouch carcinogenesis with respect to angiogenesis. Methods(Sixty) male Syrian golden hamsters were randomly divided into 4 groups(group A and B,n=20;group C and D,(n=10).) DMBA solution(0.5% in acetone) was applied topically to the right cheek pouch of male Syrian golden hamsters in group A and B,while animals in group C were painted with acetone,3 times a week for 6 weeks.Animals in group D received neither DMBA nor acetone in the first 6 weeks.Two days after the last treatment of(DMBA,) animals in group B and D received salvianolic acid B daily(10 mg/kg) by gavage,while animals in group A and C received same volume of saline,for the next 12 weeks.At the end of the experiment,animals were sacrificed and tissue samples were collected for histopathological and immunohistochemical examination. Results Salvianolic acid B significantly decreased the incidence of squamous carcinoma(P
4.Improvement and optimization of performance verification on enzyme linked immunosorbent assay for determination of hepatitis B markers
Jing SHI ; Ya ZHANG ; Lin ZOU ; Pu CHEN ; Liping ZHANG
International Journal of Laboratory Medicine 2015;(23):3369-3371
Objective To evaluate the performance of enzyme linked immunosorbent assay (ELISA) kit in detection of Hepatitis B virus(HBV) markers by using improved and optimized method ,so as to provide a practical and feasible method and reagents for clinical laboratory .Methods ELISA test was used for the detection of HBV markers .The gradient dilution method was used to e‐valuate the lower limit .The verifiation of cut off value was carried out based on clinical and laboratory standards institute (CLSI) EP12‐A2 document .Samples with cut off values were collected to evaluate the precision ,including repeatability and intermediate precision .The coincidence rates were counted through comparing the results of ELISA with those of external quality assessment and those detected by using Abbott i4000SR chemiluminescence instrument .Results The lower detection limit of HBsAg ,HBsAb , HBeAg ,HBeAb and HBcAb were 0 .2 IU/mL ,20 mIU/mL ,1 NCU/mL ,0 .75 NCU/mL and 0 .05 NCU/mL respectively .The cut‐off value(C50 )± 20% concentration included the concentration range between C5 and C95 .The within‐run coefficient of variation (CV)≤15% ,in sandwich method the between‐run CV≤25% ,in competition method the between‐run CV≤35% .The positive and negative coincidence rates in accuracy and comparing with i 4000SR all were more than 95% and all κ>0 .75 .Conclusion ELISA tests for HBV markers in our laboratory could meet the requirements of the detection performance and clinical needs .
5.Circadian rhythm changes of PGE2 levels in blood of growing rats after functional mandibular protrusion
Ya LI ; Yanping ZUO ; Xiao JI ; Ji ZOU
Journal of Practical Stomatology 2014;(6):757-759
Objective:To explore the relationship between wearing of functional appliance and systemic metabolism of PGE2 in the blood of growing rats.Methods:54 male Wistar rats aged 4-weeks were randomly divided into 3 groups(n =18):Daytime group,all-day group and control group.Self-guided functional appliance was made for mandible advancement of the rats in the 2 experimental groups.One week after appliance wearing,rats were sacrificed at the corresponding time points and blood in femoral artery was taken. PGE2 levels in the blood samples were measured,data were analysed by cosine equation.Results:Hypothesis was true in the control group(P <0.05),and was not true in both 2 experimental groups(P >0.05).Conclusion:PGE2 level in rat blood is circadian rhyth-mic,wearing of functional appliance made may disturb the rhythm.
6.Influences of two head holders on setup errors during head and neck neoplasms radiotherapy
Yan HU ; Lianghe WANG ; Xingmeng CHEN ; Ya ZOU ; Jingguo FU
Chinese Medical Equipment Journal 2017;38(3):80-82,93
Objective To compare the effects of two head holders on the setup errors during head and neck neoplasms radiotherapy.Methods Totally 30 patients were divided into groups A and B randomly and equally.Group A applied TypeSTM Extension for setup,and group B used Type-S Overlay Board,with thermoplastic film applied to fixation.Varian23IX image guided linear accelerator was involved in the therapy,and the patients underwent CBCT-guided therapy once a week in 6 weeks.The setup errors at left-right,head-foot and anterior-posterior directions were obtained by bone registration and manual checking.Results The absolute setup error at anterior-posterior direction of group A,was (2.31 ± 1.35) mm,which was significantly higher than (1.88±1.79) mm of group B (P=0.032).There were no obvious differences between the setup errors of group A and those of group B (P>0.05),and the weekly absolute errors of group A were not statistically different from those of group B (P>0.05).Conclusion There are no significant differences between the effects of the two head holders on the setup errors during head and neck neoplasms radiotherapy,and proper head holder can be selected according to clinical requirements.
7.Not Available.
Ya jing ZOU ; Jian YAO ; Wei jun KAN
Journal of Forensic Medicine 2022;38(2):293-295
8.In vitro lipolysis process and lipolysis mechanism of testosterone undecanoate type Ⅲ lipid formulations
Ya-meng WANG ; Dan-lu ZOU ; Yu LI ; Xue KE
Acta Pharmaceutica Sinica 2021;56(6):1696-1703
The study evaluates the lipolysis rate and extent of type Ⅲ lipid formulations using testosterone undecanoate as a model drug after digestion with
9.Platelet-rich plasma trigger point injection treatment for chronic achilles insertion tendonitis
Guoyou ZOU ; Weitao JIA ; Minqian ZHENG ; Xiaozu XU ; Zhengchun CAO ; Jun YIN ; Ya WU
Chinese Journal of General Practitioners 2013;12(8):657-659
Retrospective analysis was performed for the clinical data of 15 chronic insertion achilles tendinitis patients undergoing platelet-rich plasma (PRP) trigger point injection.The scores of Validated Victorian Institute of Sports Assessment-Achilles (VAS-A) and foot function index (FFI) improved greatly versus pre-treatment (all P < 0.05).Tendon insertion structure inflammation decreased significantly on magnetic resonance imaging.At the last follow-up,all patients recovered normal gait and daily activity.The trigger point injection of PRP is efficacious for chronic insertion achilles tendinopathy.
10.Platelet-rich plasma versus corticoid in the treatment of chronic lateral elbow epicondylitis
Guoyou ZOU ; Weitao JIA ; Minqian ZHENG ; Ruli ZHU ; Hongbing LIU ; Wensheng ZHANG ; Ya WU
Chinese Journal of General Practitioners 2013;12(11):916-918
A total of 52 patients with chronic lateral epicondylitis were randomly assigned into platelet-rich plasma (PRP) (n =28) or corticosteroid (n =24) group.The visual analog pain scale (VAS) and Mayo elbow score had no significant difference between two groups within 1 week post-treatment.However,VAS and Mayo elbow score improved more significantly in PRP group versus corticosteroid group at Month 1 and 6 post-treatment (P < 0.05).The PRP trigger point injection treatment of elbow lateral epicondylitis achieved clinical outcomes and it was superior to glucocorticoid treatment.