Objective To observe the clinical efficacy of acupuncture in treating cancer-related fatigue (CRF) due to spleen-kidney deficiency.Method Sixty eligible subjects with CRF due to spleen-kidney deficiency were randomized into a treatment group and a control group, 30 cases in each group. The control group was intervened by nutritional support and symptomatic management, while the treatment group was treated with acupuncture in addition to the intervention given to the control group. The Karnofsky Performance Status (KPS), Piper Fatigue Scale (PFS), and peripheral blood test were observed before and after treatment.Result The KPS scores were significantly changed after treatment in both groups (P<0.05). The PFS item scores (behavior, affect, sensory, and cognition) were significantly changed after intervention in the treatment group (P<0.05). The behavior score in the control group was significantly changed after treatment (P<0.05). After treatment, there were significant differences in comparing the PFS item scores between the two groups (P<0.05). There were significant differences in comparing the lymphocyte levels between the two groups after treatment (P<0.05).Conclusion Acupuncture is an effective method in treating CRF due to spleen-kidney deficiency, and it can up-regulate the peripheral blood cells and strengthen the immunity.

Objective To study the value of the neuron-specific enolase(NSE) in the serum of patients with cerebral stroke in diagnosis, seriousness and evaluation of prognosis. Methods The levels of NSE in the serum of 77 patients with cerebral stroke(36 cases with hemorrhage, 41 cases with infarction) were measured by radio-immunoassay (RIA)method.Results The serum leves of NSE both in cerebral infarction and cerebral hemorrhage patients were obviously higher than those of the control group( P 0.05). In the first week there was a positive correlation between NSE serum level and neurological deficit scores,the volume of haemorrhage, the volume of infarction( P 0.05) . Conclusion The NSE level in serum may be served as a objective index of seriousness and early diagnosis in stroke, but no helpful in differential diagnosis and evaluation of prognosis.

Objective To study the antiangiogenesis effect of active components T3 and M2 from single Chinese herbal medicine No.6 and No.10 with invigorating the circulation of blood in Chinese medicine theory. Methods human vascular endothelial cell (EC) was cultivated in vitro, the MTT assay and CAM experiment were carried out. Results T3 and M2 showed very significant inhibitory effect on EC (P

Panaxatriol saponin (PTS), isolated from Panax pseudo-ginseng var. notoginseng, 1~ 4mg/ml inhibited rabbit platelet aggregation induced by ADP,collagen and arachidonic acid respectively in vitro. In rat,PTS 75 ~300mg/kg intraduodenally inhibited dose-dependently platelet aggregation induced by these inducers, andalso inhibited platelet thrombonane A2 release induced by collagen,but did not affect the formation of aorticwall PGI2,PTS 50~200mg/kg significantly inhibited experimental thrombosis in rats. These results showed that the anti-thrombosis action of PTS may be due to its inhibitory action on platelet aggregation and TXA2re lease

"CapFinder" technology,which can be used to clone the full length of 5′ UTR sequence of mRNA,was described.This technology used the terminal transferase activity of certain MMLV RT variants that added 3-5 residues(predominantly dC) to the 3′end of the first-strand cDNA exhibited when MMLV RT reached the 5′cap structure of mRNA.In the reverse reaction system containing GGG oligo,the terminal transferase activity was harnessed by the GGG oligo whose terminal stretch of dG residues can anneal to the dC-rich cDNA tail and serve as an extended template for RT.After RT switch templates from the mRNA template to the GGG oligo,a complete cDNA copy of the original RNA was synthesized with the additional GGG oligo sequences at the end.5′UTR of mRNA can be amplified with GGG oligo as forward primer and a gene-specific reverse primer.5′UTR of Bt toxin receptor E-Cadherin gene in midgut of cotton bollworm was cloned.

Adult ; Burkitt Lymphoma ; genetics ; Chromosome Duplication ; Chromosomes, Human, Pair 1 ; Chromosomes, Human, Pair 14 ; Chromosomes, Human, Pair 8 ; Female ; Humans ; Leukemia, B-Cell ; genetics ; Male ; Middle Aged ; Retrospective Studies ; Translocation, Genetic

Objective:To investigate the correlation between preoperative blood platelet-to-lymphocyte ratio (PLR) and clinico-pathological features, as well as the effect of PLR on the prognosis of non-small cell lung cancer (NSCLC) patients after surgical resec-tion. Methods:Retrospective analysis was performed for 255 cases with histologically confirmed NSCLC that underwent curative re-section from January 2004 to December 2007. All patients were classified into two groups based on the median value of PLR. The rela-tionship between PLR and clinicopathological features was studied. Univariate and multivariate analyses were performed to assess the prognostic effect of preoperative PLR. Results:The median value of preoperative PLR was 130 (range:45.45 to 272.66). Based on the cut-off value of 130, all patients were divided into two groups:low PLR (≤130, n=127) and high PLR (>130, n=128). PLR was corre-lated with tumor site, T stage, and clinical stage. Five-year survival rates of low and high PLR patients were 49.6%and 33.6%, respec-tively, which indicated a statistically significant difference (χ2=12.577, P<0.001) between the two groups. Univariate analysis showed that smoking status, histological differentiation, clinical stage, T stage, N stage, postoperative adjuvant therapy and PLR were associat-ed with survival (P<0.05 for all). Multivariate analysis identified N stage, postoperative adjuvant therapy, and PLR as independent prog-nostic factors of all the patients. In addition, stratified analysis showed that the five-year survival rate of the low PLR group was higher than that of the high PLR group with or without lymph node metastasis, and the differences were statistically significant (P=0.020 and 0.037). Conclusion:An elevated blood preoperative PLR indicates poor prognosis in NSCLC patients. Preoperative PLR is an indepen-dent prognostic factor of NSCLC after curative resection.

Objective To investigate the correlation between the results of HBsAg positive results among different detection systems,and provide reference for the analysis of clinical test results and the publication of the report.Methods The HBsAg positive serum specimens with the quantitation result lower than 80 COI were detected by Roche Cobas e602 electrochemical luminescence analyzer.All the specimens were also detected by Roche Modular e170 electrochemical luminescence analyzer.The differences of detecting results were compared and performed the linear correlation analysis.Results The experimental results of two detection systems are R2 =0.933.The positive coincidences of Group A,B,C,D and E were 60.60％,92.72％,96.66％,96.66％ and 100％,respectively.The positive coincidence of the males was 87.66％,while the positive coincidence of the females was 70.96 ％.The positive coincidence of the males was significantly higher than the females (P＜0.05).Conclusion The HBsAg detecting results of Roche Cobas e602 and Roche Modular e170 electrochemical luminescence analyzer had high correlation.The results higher than 10 COI had 100％ positive coincidence rate,however the results between 1 and 10 COI were not.The result between 1 and 10 COI may lead to controversial results,suggestions for further checks.

Chlorine ; Computer Simulation ; Computers, Analog ; Diffusion ; Software

Noise, Occupational ; prevention & control ; Occupational Health Services ; standards ; Threshold Limit Values