Clavicle ; injuries ; surgery ; Fracture Fixation, Internal ; methods ; Fractures, Bone ; complications ; surgery ; Humans

OBJECTIVE To learn the effect of performance management to enhance the proper administration of antibiotics in hospital.METHODS The performance management was applied in the proper administration of antibiotics usage.It included perfected regulation system,established the standard of the performance management,enhanced monitoring,analyzed the popular trend of drug-resistant bacteria,educated the medical staff and guided their appropriate antibiotics usage.RESULTS After taking the performance management in the administration of antibiotics,the rate of the antibiotics usage decreased from 55.1% to 44.2% and the rate of hospital infection decreased from 5.26% to 4.69%.The drug-resistant bacteria ratio obviously repressed.CONCLUSIONS Application of the performance management(a modern science management measure) in the proper administration of antibiotics usage is effective.

Objective To investigate the clinic effect of hypocellular acute myelogenous leukemia (AML) patients with CAG regimen. Methods 29 cases with hypocellular AML were treated with CAG regimen. Results There were 14 cases achieved complete remission (CR), accounting for 48.3 %; 7 cases partial remission (PR), accounting for 24.1%. The total effective rate was 72.4 %, inducing 7 cases of NR, one case dead in early time. Conclusion CAG regimen has been proved to be effective in shortening the period of reduced peripheral blood granulocytes and decreasing the death rate related to chemotherapy, hence,a secure and effective therapy in treating hypocellular AML.

Objective:To explore the inhibitory effect and mechanism of photocarcinorin (PSD-007) photodynamic therapy (PDT) on human nasopharyngeal carcinoma transplanted tumors in nude mice.Methods:A total of 50 transplanted tumor nude mice models of human nasopharyngeal carcinoma were established and randomly divided into 5 groups: control group (group A), simple PSD-007 group (group B), simple light group (group C), local injection of PSD-007 + light group (group D), intraperitoneal injection of PSD-007 + light group (group E) using the method of random number table, 10 mice in each group. After 7 days of treatment, the tumor mass and tumor volume of nude mice in each group were measured and the tumor inhibition rate was calculated. HE staining was used to detect the histopathological changes of tumors in nude mice. Western blotting was used to detect the expressions of autophagy-related gene Beclin 1, microtubules associated protein 1 light chain 3-β (LC3) and phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/Akt/mTOR) signaling pathway related proteins. Real-time fluorescent quantitative PCR was used to detect LC3, Beclin 1 mRNA expressions in tumor tissues.Results:After treatment, the tumor mass of nude mice bearing human nasopharyngeal carcinoma in group A, B, C, D and E were (2.05±0.18) g, (2.02±0.20) g, (2.04±0.15) g, (0.43±0.11) g and (0.94±0.12) g, and the tumor volumes were (1.11±0.13) cm 3, (1.18±0.16) cm 3, (1.13±0.14) cm 3, (0.51±0.07) cm 3and (0.65±0.10) cm 3, and there were statistically significant differences among the 5 groups ( F=236.749, P<0.001; F=62.418, P<0.001). Compared with group A, B and C, the tumor mass and tumor volumes of nude mice in group D and E were significantly reduced, and there were statistically significant differences (all P<0.001); compared with group E, the tumor mass and tumor volume of nude mice in group D were significantly reduced ( P<0.001; P=0.023). The tumor inhibition rates of group B, C, D and E were (1.07±0.11)%, (0.55±0.06)%, (79.11±0.06)% and (54.05±0.08)%, with a statistically significant difference ( F=235.987, P<0.001), compared with group B, C and E, group D had the most obvious tumor suppressing effect (all P<0.05). HE staining results showed that compared with group A, B and C, group D and E had larger tumor necrosis areas, more inflammatory cell infiltration, more vacuolar degeneration, and obvious nuclear shrinkage. The tumor necrosis degree in group D was higher than that in group E. The relative expressions of PI3K protein of group A, B, C, D and E were 1.01±0.06, 1.00±0.05, 1.01±0.05, 0.23±0.02, 0.48±0.04, p-Akt/Akt protein relative expressions were 0.66±0.06, 0.65±0.05, 0.64±0.05, 0.06±0.02, 0.17±0.02, p-mTOR/mTOR protein relative expressions were 1.06±0.06, 1.01±0.06, 1.01±0.06, 0.30±0.02, 0.45±0.04. The protein relative expression ratios of LC3 Ⅱ/LC3 Ⅰ were 0.85±0.05, 0.83±0.05, 0.83±0.06, 0.22±0.02, 0.41±0.04, and Beclin 1 protein relative expressions were 0.66±0.06, 0.64±0.05, 0.64±0.06, 1.67±0.07, 1.02±0.05, LC3 mRNA relative expressions were 0.98±0.29, 0.92±0.25, 1.02±0.26, 3.76±0.28, 2.38±0.28, and Beclin 1 mRNA relative expressions of were 1.11±0.40, 1.19±0.29, 1.16±0.24, 6.84±0.54, 2.94±0.48. There were statistically significant differences ( F=190.160, P<0.001; F=160.014, P<0.001; F=160.183, P<0.001; F=119.964, P<0.001; F=186.257, P<0.001; F=211.089, P<0.001; F=374.835, P<0.001). Compared with group A, B and C, PI3K, p-Akt/Akt and p-mTOR/mTOR protein relative expression levels and protein relative expression ratios of LC3 Ⅱ/LC3 Ⅰ in nude mice tumors of group D and E were significantly reduced, and LC3 mRNA, Beclin 1 protein and mRNA relative expression levels were significantly increased, with statistically significant differences (all P<0.001); PI3K, p-Akt/Akt and p-mTOR/mTOR protein relative expression levels and protein relative expression ratio of LC3 Ⅱ/LC3 Ⅰ in nude mice tumors of group E were significantly higher than those of group D, while Beclin 1 protein relative expression levels, LC3 and Beclin 1 mRNA relative expression levels were reduced (all P<0.05). Conclusion:PSD-007 PDT has an inhibitory effect on human nasopharyngeal carcinoma transplanted tumors in nude mice, which may be related to the inhibition of PI3K/Akt/mTOR signaling pathway activation and the promotion of autophagy. Compared with intraperitoneal injection, local injection of PSD-007 PDT is more effective.

Bone Diseases ; Humans ; Hyoid Bone ; Male ; Middle Aged ; Syndrome

Aged, 80 and over ; Humans ; Laryngeal Diseases ; Male ; Neck ; Pharynx

Objective To examine the serum high-sensitivity C-reactive protein(hs-CRP)levels in subjects with type 2 diabetic mellitus(DM)or DM accompanied by cerebral infarction(DM+CI),and the relationship be- tween the serum high-sensitivity C-reactive protein(hs-CRP)levels,and to investigate risk factors of cerebral infarc- tion in patients with type 2 diabetic mellitus.Methods The serum high-sensitivity C-reactive protein(hs-CRP)lev- els were measured in 50 DM patients,50 DM+CI patients and 30 healthy controls by ultra-sensitive immunoassay. hs-CRP values and its relationship with cerebral infarction in patients with type 2 diabetic mellitus and other factors, such as age,BMI,TG,etc,were analyzed.Results Univariate analysis revealed significant differences in hs-CRP concentrations between controls[(0.73?0.46)mg/L]and subjects with DM[(2.26?1.38)mg/L],or subjects with DM+CI[(3.82?2.67)mg/L](P

Objective: To investigate the changes of the small G protein RhoB expression in scalded rat livers and heat-stressed human prostate cancer cell line PC-3, so as to discuss the influence of heat stress on expression of RhoB in vitro and in vivo. Methods: Third degree burns of 30% total body surface area (TBSA) model was established with SD rats on the back. The expression of RhoB mRNA and protein in the liver was determined by RT-PCR and Western blot 2 h, 4 h, 8 h and 16 h (n=6) after scalding; the liver tissues of normal rats were taken as control (n=6). PC-3 cells were allowed to recover for 0.5 h, 1 h, 2 h, 4 h and 8 h in a cellular heat stress model and the expression of RhoB in mRNA and protein were determined by RT-PCR and Western blot, respectively; untreated PC-3 cells were taken as control. Results: The expression of RhoB mRNA in the livers peaked 4 h after scalding, being about 3.2 folds that of the control group (P<0.01); the expression began to decline 8 h after scalding. The expression of RhoB protein peaked 8 h after scalding, significantly higher than that of the control (P<0.01). RhoB mRNA level began to increase 2 h after heat stress treatment and peaked at 4 h, being about 2. 8 folds that of the control (P<0.01). The expression of RhoB protein reached its maximum at 8 h after heat stress treatment (P<0.01). Conclusion: Heat stress can up-regulate the expression of RhoB mRNA and protein in vivo and in vitro.

Objective: To construct a vector encoding short hairpin RNA(shRNA) targeting annexin H and to observe its influence on annexin II expression. Methods: Annexin II-targeted hairpin RNA1 and RNA2 were devised according to the Gen-Bank annexin II sequence. Meanwhile, a nonspecific sequence was taken as negative control. The oligonucleotide strands of DNA fragments encoding the above shRNAs were synthesized. After annealing of the complementary strands, the DNA fragments were cloned into human pGenesil1 plasmid followed by amplification in E. coli and sequencing. The 3 pGenesil1 constructs were then transfected into HEK293 cells and the expression of annexin II was examined by real-time fluorescence quantitative RT-PCR and Western bolt. The non-transfected cells were taken as blank control. Results: Restrictive enzyme digestion and sequencing verified that the 3 recombinants (pGenesil1-annexin II shRNA1, pGenesil1-annexin II shRNA2, and pGenesil1-negative shRNA) were correct. The annexin II expression was significantly lower in HEK293 cells transfected with pGenesil1-annexin II shRNA2 compared with those transfected with pGenesil1-annexin II shRNA1, pGenesil1-negative shRNA, and blank control (P<0.05); and there was no significant difference between the latter 3. Conclusion: We have successfully generated an annexin II-targeted shRNA construct, which can significantly inhibit the expression of annexin II.