2.Relationship between oxidized low density lipoprotein and C-reactive protein in patients with acute coronary syndrome
jing-jing, WEI ; ya-chen, ZHANG ; jun, QIAN
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(10):-
Objective To investigate the changes of oxidized low density lipoprotein(oxLDL) and C-reactive protein(CRP) and their correlation in patients with acute coronary syndrome(ACS),and explore their roles in the pathogenesis of ACS. Methods The plasma levels of oxLDL and CRP were measured in 95 patients with ACS and 66 control subjects by ELISA. Results oxLDL and CRP levels were significantly higher in patients with ACS than those in control group(P
3.Improvement and optimization of performance verification on enzyme linked immunosorbent assay for determination of hepatitis B markers
Jing SHI ; Ya ZHANG ; Lin ZOU ; Pu CHEN ; Liping ZHANG
International Journal of Laboratory Medicine 2015;(23):3369-3371
Objective To evaluate the performance of enzyme linked immunosorbent assay (ELISA) kit in detection of Hepatitis B virus(HBV) markers by using improved and optimized method ,so as to provide a practical and feasible method and reagents for clinical laboratory .Methods ELISA test was used for the detection of HBV markers .The gradient dilution method was used to e‐valuate the lower limit .The verifiation of cut off value was carried out based on clinical and laboratory standards institute (CLSI) EP12‐A2 document .Samples with cut off values were collected to evaluate the precision ,including repeatability and intermediate precision .The coincidence rates were counted through comparing the results of ELISA with those of external quality assessment and those detected by using Abbott i4000SR chemiluminescence instrument .Results The lower detection limit of HBsAg ,HBsAb , HBeAg ,HBeAb and HBcAb were 0 .2 IU/mL ,20 mIU/mL ,1 NCU/mL ,0 .75 NCU/mL and 0 .05 NCU/mL respectively .The cut‐off value(C50 )± 20% concentration included the concentration range between C5 and C95 .The within‐run coefficient of variation (CV)≤15% ,in sandwich method the between‐run CV≤25% ,in competition method the between‐run CV≤35% .The positive and negative coincidence rates in accuracy and comparing with i 4000SR all were more than 95% and all κ>0 .75 .Conclusion ELISA tests for HBV markers in our laboratory could meet the requirements of the detection performance and clinical needs .
4.Preliminary study on anti-fatigue effects of enzymatic hydrolyzed animal protein extracted from Pinctada martensii
Chaohua ZHANG ; Ya LIU ; Ping YANG ; Jing ZHANG ; Hongwu JI
Chinese Journal of Marine Drugs 1994;0(04):-
Objective To study the anti-fatigue effects of enzymatic hydrolyzed protein from Pinctada martensii.Methods Swimming time,contents of liver glycogen and serum urea nitrogen after swimming were determined.Results Three dosages of EPA were all able to prolong swimming time and decrease liver glycogen consumption and serum urea nitrogen content after exhausted swimming in various degree.Conclusion The EAP has evident anti-fatigue effects.
8. Expression of salt-taste herbs and their applications in clinical compatibility
Chinese Traditional and Herbal Drugs 2016;47(16):2797-2802
Authors summarized the connotation of traditional Chinese medicine (TCM) theory on the salty in five flavors of TCM and the distribution of base resource of salt-taste herbs. At the same time, the correlation between salt-taste and four properties, meridian, and other herb property theory was analyzed. Then, the authors elaborated the gustatory receptor, taste representation, and research of material basis of salt-taste. Finally, the authors described the clinical applications of compatibility methods of salt-taste herbs, which will provide the references to guide the clinical applications of the theory on salt-taste herbs better.
9. Expression of sweet-taste of Chinese materia medica and its application in clinical compatibility
Chinese Traditional and Herbal Drugs 2016;47(4):533-539
The connotation of traditional Chinese medicine and base resource of sweet-taste herbs were summarized. At the same time, the distribution between sweet-taste herbs and four properties, meridian entry, and other herb property theory was analyzed. Then, the gustatory receptor, taste representation, and research on material basis of sweet-taste herbs were elaborated. Finally, the clinical applications of compatibility methods of sweet herbs were described, which will provide the references to guide clinical applications of the theory of bitter-taste herbs better.
10. Expression of bitter taste of Chinese materia medica and its application in clinical compatibility
Chinese Traditional and Herbal Drugs 2016;47(2):187-193
The connotation of traditional Chinese medicine, source, and medicinal parts of bitter-taste Chinese materia medica (CMM) were summarized. And authors analyzed the relationship between bitter taste and four properties, meridian entry and other herb property theory as well as chemical ingredients. Then, authors described the use of electronic tongue of modern bionic technology in material characterization of bitter taste, and proposed the idea to split and characterize bitter-taste CMM which is based on systematic chemical separation analysis method. Finally, authors explored the clinical applications of compatibility methods of bitter-taste CMM, which will provide the references to guide clinical applications of the theory of bitter-taste CMM better.