Aged, 80 and over ; Humans ; Laryngeal Diseases ; Male ; Neck ; Pharynx

OBJECTIVE: To synthesis chitosan/β-glycerophosphate (CS/β-GP) thermosensitive hydrogel containing multi-walled carbon nanotubes-polyethyleneimine (MWCNTs-PEI) complexes and to lay a foundation for further research of dual slow-release delivery system. METHODS: Chitosan thermosensitive hydrogel containing MWCNTs-PEI was prepared by MWCNTs-PEI dispersed to the chitosan thermosensitive hydrogel. As the indicator of the gelling time, the experiment studied the effect of β-GP concentration, pH, temperature and MWCNTs-PEI composite quality on the thermosensitive chitosan hydrogel, and then it was charactered by using transmission electron microscopy (SEM), infrared spectrometer(IR), and initially investigated in vivo compatibility. RESULTS: The dynamic rheology method investigated the gelling temperature were about 37.0°C. Within a certain range, the gelling time of thermosensitive chitosan hydrogel was shortened with the increase of concentration of β-GP, pH, temperature, and the quality of MWCNTs-PEI complexes, and they could be transformed into the hydrogel in vivo. The addition of MWCNTs-PEI complex didn't react chemically with the thermosensitive chitosan hydrogel and significantly make the holes of the chitosan thermosensitive hydrogel smaller by SEM and FT-IR, eventually leading to the swelling rate and the corrosional ratio decrease. CONCLUSION: Chitosan/β-glycerophosphate thermosensitive hydrogel containing amino-carbon nanotubes has a rapid gelation and good temperature-sensitivity, which can serve as a good double sustained-release carrier.

OBJECTIVE: To synthesize water-soluble multi-walled carbon nanotubes-polyethyleneimine (MWCNTs-PEI) composite with low cytotoxicity and to lay a foundation for further research of loading drugs with carbon nanotubes. METHODS: MWCNTs-PEI composite was prepared by modifying carboxylated multi-walled carbon nanotubes(MWCNTs-COOH) with polyethylene (PEI), and then the composite was characterized by transmission electron microscopy, infrared spectra, UV spectra and thermalgravimetric analysis. The cytotoxicity of the composite on PC12 cells was measured by methyl thiazolyl tetrazolium(MTT) assay to preliminarily evaluate its biocompatibility. RESULTS: The dissolubilities of MWCNTs-PEI and MWCNTs-COOH complexes were respectively 1.009 and 0.0601 mg · mL-1, and the former was about 16 times of the latter. The cytotoxicity of MWCNTs-PEI composite on PC 12 cells was significantly milder than that of MWCNTs-COOH composite as indicated by MTT assay (P < 0.05). CONCLUSION: MWCNTs-PEI composite not only improves the dispersibility of carbon nanotubes, but also reduces its in vitro cell toxicity.

Objective To study the effect of transcatheter arterial chemoembolizafion (TACE) on inhibitor apoptosis protein-Survivin, Survivin mRNA and proliferating cell nuclear antigen (PCNA). Methods Group A of hepatocelluar carcinoma with preoperative TACE (n = 26), group B of hepatocelluar carcinoma without any interven-tional therapy (n = 30) ,group C of Normal liver (n = 16) were divided. The expression of Survivin mRNA and the expression of Survivin and PCNA were detected. Result ①The positive Survivin mRNA expression in group A and the grey difference were lowere than that in group B (P < 0.05) ; ②The positive Survivin expression and the grey difference in group A were lower than that in group B (P < 0.05) ; ③The proliferating index (PI) of carcinoma tis-sure in group B was higher in group A and group C (P < 0.05 for each). Conclusion There is a high positive ex-pression of Survivin mRNA and Survivin, which may be decreased by TACE and then the proliferation of hepatocellu-lar carcinoma cell is surpressed.

Adolescent ; Child ; Child, Preschool ; Female ; Herpesvirus 4, Human ; Humans ; Infant ; Infectious Mononucleosis ; diagnosis ; pathology ; virology ; Liver ; pathology ; Male

To sum up the experience of microneurosurgical treatment of cavernous sinus tumor, an retrospective analysis was made for 26 cases of cavernous sinus tumor admitted to our hospital from January, 1990 to June, 2001. All the patients received the modified microneurosurgical operation, and the operation was performed in 6 of them by the help of nerve guidance.The tumors were totally removed in 19 patients, and partially removed in the other 7 patients. No patient died, and cranial nerve injury was ameliorated in 14 cases, and some new cranial nerve injuries were found in 6 patients after operation. No recurrence or regrowth of tumor was found during the following up from 8 months to 12 years. It is crucial to master the microneurosurgical techniques for the reduction of operative complications.

Objective To investigate the long-term effect of recombinate human growth hormone(rhGH) on the immune function in patients with liver cancer. Methods Fifty patients with hepatoma who underwent hepatectomy were randomly divided into two groups:(1)Control group;(2)rhGH treatment(GH) group. All patients were treated with TPN for five days postoperatively,and additionally the patients in the GH group received rhGH(8U/d IH) in the evening for seven days. Results IgG,IgM,CD3,CD4 in the control group and CD8 in the GH group were significantly lower on day 10 postoperatively than that on preoperative days. IgG in the GH group significantly increased on day 15. The 1 year and 3 year survival rate and recurrent rate were not different between the two groups. Conclusions rhGH therapy can improve the immune function of patients with hepatoma who underwent hepatectomy and has no side effect on the long term survival and recurrence.

A moderately thermoacidophilic iron-oxidizing bacterium,designated as strain MLY,was isolated from a coal spoil heap in China.The optimum of temperature for growth is 50℃～54℃.The optimum of pH is 1.2～1.4.The strain MLY is facultative autotroph and grows heterotrophically on yeast extract.It is able to oxidize ferrous iron(Fe 2+ ),pyrite(FeS 2),and elemental sulfur(S 0) autotrophically and mixotrophically in the presence of yeast extract.Autotrophic oxidation of elemental sulfur is relative weak.The comparison of ferrous iron and pyrite oxidation between strain MLY and A10 Thiobacillus ferrooxidans,strain indicated that MLY is one time faster than A10.

Existing reporting guidelines for systematic reviews/Meta-analysis (SRs/MAs) cannot meet the requirements of clinical practice and scientific research, so based on evidence, methods and thoughts on establishing reporting guidelines for TCM-featured acupuncture systematic reviews/Meta-analysis were proposed. Through literature analysis, according to evidence-based principle, preliminary ideas on methodology of establishing reporting guidelines for TCM-featured acupuncture systematic reviews /Meta-analysis were proposed. With consensus as one main research method, it was proposed that in the consensus that was selected and established by experts, the pro- portion of Chinese scholars should be increased to fully declare the opinions of Chinese acupuncturists, and by verification of practice and application, the reporting guidelines for TCM-featured acupuncture systematic reviews/Meta-analysis can be finally made.
Acupuncture Therapy ; methods ; standards ; Evidence-Based Medicine ; methods ; standards ; Humans ; Medicine, Chinese Traditional ; methods ; standards

Objective To explore the personality characteristics of abused children in order to reduce the incidence of child abuse.Methods Two hundred and ninty five middle school students were investigated with general questionnaire and Eysenck Personality Questionnaire of children. Eighty six students experiencing child abuse (CA) last year as study group and one hundred and ninety six non-abuse children as controls (NCA) were analyzed by means of Eysenck Personality Questionnaire of children.Results The score of neuroticism in CA group was significantly higher than that in the control group (55.62±10.60/52.65±10.98,t=-2.114 P=0.035). The score of lie in CA group was significantly lower than that in control group (42.21±9.87/46.04±9.20,t=3.184 P=0.002). On the impact of different sex, the psychoticism score of male was significantly higher than that in the control group(52.37±11.49/48.04±9.97,t=-2.227 P=0.028), and the lie score was significantly lower than that in control group(41.03±9.18/46.18±8.79,t=3.125 P=0.002).The scores of those in the female were not significant.Conclusions There is a close association between the unstable emotion and child abuse in children, so training emotional self-control and emotional expression of children might be a intervention strategy in the future. In addition, the frequency of lie in children is probably one of factors that determine whether children are abused or not.