Hepatitis B virus exists in the form of quasispecies.Quasispecies are an important basis for the pathogenesis and drug resistance of hepatitis B virus.Researches for quasispecies could contribute to evaluating the progress of disease, predicting drug resistance, adjusting the therapeutic regimen and performing individual treatment.

China ; Clinical Laboratory Techniques ; Laboratories ; statistics & numerical data ; Occupational Health ; Quality Control

Acute Coronary Syndrome ; Humans

Aged ; Chondrosarcoma ; Humans ; Laryngeal Neoplasms ; Male ; Middle Aged

Actins ; metabolism ; Angiotensins ; pharmacology ; Animals ; Breast Neoplasms ; blood supply ; drug therapy ; metabolism ; pathology ; Cell Proliferation ; Female ; Fibroblasts ; metabolism ; pathology ; physiology ; Humans ; Interferon-gamma ; therapeutic use ; Neoplasm Invasiveness ; Neoplasm Metastasis ; Neovascularization, Pathologic ; Prognosis ; Transforming Growth Factor beta1 ; metabolism

Breast Neoplasms ; metabolism ; pathology ; Cell Polarity ; Epithelial Cells ; metabolism ; pathology ; Epithelial-Mesenchymal Transition ; Eye Proteins ; metabolism ; Female ; Humans ; Membrane Proteins ; metabolism ; Nerve Tissue Proteins ; metabolism ; Receptors, Proteinase-Activated ; metabolism ; Tumor Suppressor Proteins ; metabolism

BACKGROUND: Current research of mechano growth factor (MGF) mainly focuses on the muscles and nerve damage and repair, and it has bean confirmed that MGF can promote muscle cell hypertrophy and nerve repair significantly. Regarding its role in fracture healing is unclear. OBJECTIVE: To investigate the effect of MGF on radial fracture healing in rabbits. METHODS: By using random digital table method, 12 New Zealand rabbits were divided into 3 groups: blank control group, low-dose MGF group and high-dose MGF group. The models with 5 mm bone defect were produced in the middle of the left radius in rabbits. At 3 days after the surgical operation, the defective areas were given 0.2 mL PBS or 0.2 mL MGF (0.36 and 0.72 g/L) injected into the ends of fracture areas, respectively, once per day for continuous 5 days. At 4, 6, 8 weeks after operation, X-ray photography was used to evaluate the healing of fracture, and the histological examinations were performed at the 8~(th) weak to observe the call morphology at the fracture lesion. RESULTS AND CONCLUSION: At 1 day after operation, the activities of rabbits were reduced, with slightly reduced food intake, at 2 days they almost recovered to normal activities and diet. At 3 days, the surgical incision slightly swelled with a small amount of bleeding and without obvious signs of infection. All 12 rabbits entered the final analysis. X-rays showed that two fracture ends have basically combined in the high-dose MGF group at 4 weeks post-surgery, cortical bone was continuous and fracture lines were unclear. At 6 weaks the bone medullary cavity almost run through and fully run through at 8 weeks. The healing time in the high-dose MGF group was remarkably shorter than that in blank control group and low-dose MGF group, the healing was in high quality. At 8 weeks after operation, a large number of osteoid tissues were observed in the blank control group, a small amount of woven bone formed, at a transition period from the fibrous bone callus to the bony bone callus; a large number of woven bone formed inthe low-dose MGF group, at bony bone callus period; in the high-dose MGF group, a large number of woven bones converted into mature lamellar bone, at the callus rebuilding phase, which was consistent with imaging results. It is indicated that MGF can accelerate fracture healing significantly in a rabbit model and shows a dose-dependent manner in a certain range.

Objective: To examine the characteristics of childhood traumatic experiences in borderline personality disorder (BPD) in college students. Methods: The Personality Diagnosis Questionnaire-~(4+) (PDQ-~(4+)), Personality Disorder Interview- Ⅳ (PDI - Ⅳ) and Childhood Trauma Questionnaire -28 Short Form (CTQ-SF) were administered to 3227 college students of sophomore and junior. Results: (1) In the investigation of PDQ-~(4+), 31 subjects with childhood traumatic experiences (0.96%) were diagnosed as BPD, and the total score of BDL sub-scale was (2. 62 ± 1.70), including 18 females and 13 males. In CTQ test, the scores of bad environment in females were significantly higher than that in males [(13.63±4.54) vs. (9.83±1.95), P<0.01] . (2) Subjects with BPD got higher scores than normal controls in CTQ-SF, such as the emotional abuse [(2.11 ±0.77) vs. (1.66±0.49), P<0.01] .Conclusion: college students with borderline personality disorder mostly have d different childhood traumatic experiences, and there exists a sex difference.

OBJECTIVE:To establish a method for the simultaneous determination of acetic acid and succinic acid in Banxia syrup. METHODS:RP-HPLC was performed on the column of GL InterSustain-C18 with mobile phase of 0.03 mol/L ammonium di-hydrogen phosphate buffer solution(pH2.0)-methanol(gradient elution)at a flow rate of 0.8 ml/min,the detection wavelength was 210 nm,column temperature was 30 ℃,injection volume was 10 μl. RESULTS:The linear range was 0.020 98-0.209 8 μg/ml for acetic acid(0.999 9)and 12.04-120.4 μg/ml for succinic acid(r=0.999 9);limits of quantification were 0.15,18.24 ng,limits of detection were 0.045, 5.53 ng;RSDs of precision, stability and reproducibility tests were lower than 2%;recoveries were 97.45%-101.68%(RSD=1.39%,n=9) and 98.31%-101.08%(RSD=1.01%,n=9). CONCLUSIONS:The method is accurate and rapid,and suitable for the simultaneous determination of acetic acid and succinic acid in Banxia syrup.

OBJECTIVE:To prepare multivesicular liposomes-sodium polymannuronate microspheres(MVLs-Alg)for protein drugs and to study the physico-chemical property and elementary stability of MVLs-Alg.METHODS:MVLs and Alg were used as matrix and BSA as a model protein drug,MVLs-Alg microspheres were prepared by interior/gelatin method.The relationship between MVLs and Alg was analyzed by differential thermal analysis and the effect of temperature(4,25,40 ℃)on entrapment efficiency and peroxide value of phospholipid in base material was investigated.RESULTS:The MVLs-Alg microspheres were spherical.Analyzed by DTA,it was considered that physical adsorption between MVLs and Alg was potentially existed.Storing for 90 days at 4,25,and 40 ℃ respectively,the encapsulation efficiency of the MVLs-Alg microsphereswere were 85.36%,63.75%,and 50.49%,respectively.The peroxide value of phospholipid increased increasingly,but not significant as compared with MVLs.CONCLUSIONS:The stability of MVLs-Alg microspheres was heightened compared with that of MVLs and which can be used for the delivery of protein drugs.