AIM: To determine the HPLC fingerprint of ultramicro decoction piece of Radix paeoniae Alba. METHODS: HPLC was used to analyze the extracts of ultramicro decoction piece of Radix paeomae Alba from 10 different sources. RESULTS: The fingerprint of ultramicro decoction piece of Radix paeoniae Alba was composed of 20 peaks,among which there were 10 characteristic peaks. CONCLUSION: The fingerprmt can be used to control the ultramicro decoction piece of Radix paeoniae Alba qualities.

[Objective] To observe the clinical effect ofWen Yang Zhu Shui Formulaecombined with moxa salt packets in the treatment of malignant ascites. [Methods] 61 patients were randomly divided into an observation group(n=31) and a control group(n=30). The patients in the control group were treated with western medicine, while patients in the observation group, based on the treatment of control group, were treated with Wen Yang Zhu Shui Formulaecombined with moxa salt packets for one time a day for two weeks. The effective rates for malignant ascites and Karnofsky performance score(KPS) were compared in the two groups. [Results] The effective rate for malignant ascites in the observation group(80.64%) was higher than that in the control group (53.33%), P<0.05. The observation group showed significantly higher score of KPS than patients of the control group( P<0.05). [Conclusion]Wen Yang Zhu Shui Formulaecombined with moxa salt packets can effectively reduce the malignant ascites.

Actins ; metabolism ; Adult ; Desmin ; metabolism ; Diagnosis, Differential ; Histiocytoma, Malignant Fibrous ; pathology ; Humans ; Immunohistochemistry ; Leiomyoma ; pathology ; Leiomyosarcoma ; metabolism ; pathology ; surgery ; Male ; Mediastinal Neoplasms ; metabolism ; pathology ; surgery ; Vimentin ; metabolism

Objective To explore the correlation between expression of VEGF in colorectal cancer and pathological factors.Methods The expression of VEGF in 99 cases with colorectal cancer was examined by immunohistochemical staining.Results It showed that the expression of VEGF in colorectal cancer was correlated with depth of invasion and lymph node metastasis and was not correlated with tumor region,and histologic type.The frequency of positive cases in patients with lymph node metastasis was significantly higher than that in patients without lymph node metastasis(P

Objective To evaluate the anti-cervical carcinoma cells effects of oncolytic adenovirus d/1520 and Ad-Endo in vitro,and investigate the influence of d/1520 on the Endostatin expression directed by Ad-Endo in cervical carcinoma cells.Methods After cervical carcinoma cells were infected with d/1520 or/and Ad-Endo,the cytopathic effects (CPE) were observed,and the cytotoxicities were measured by MTT assay.Endestatin concentrations were measured by ELISA.Results d/1520 can replicate in cervical carcinoma cells and induce cytopathie effects; In MTT assay,Ad-Endo hardly inhibited the growth of cervical carcinoma cells,while d/1520 inhibited efficiently the proliferation of cervical cancer cells.And the inhibitory rates in Ad-Endo + d/1520 group were higher than that in d/1520 group.Ad-Endo expressed Endestatin protein in cervical cancer cells,while Endostatin concentrations in Ad-Endo+dl1520 group were much higer than those in Ad-Endo group.Conclusions dl1520 can kill cervical carcinoma cells in vitro,and this eytotoxieity can be enhanced by combining with Ad-Endo.The expression of Endostatin directed by Ad-Endo in cervical cancer cells can be dramatically increased by dl1520.

Female ; Follow-Up Studies ; Hearing Loss ; etiology ; Hearing Tests ; Humans ; Infant, Newborn ; Male ; Risk Factors

Humans ; Infant Nutritional Physiological Phenomena ; standards ; Infant, Newborn ; Nutritional Support ; standards ; Practice Guidelines as Topic

Objective To induce adult adult adipose-derived stem cells(ADSCs) in vitro to differentiate into neuronal-like cells,and to analyze the features of their cell morphology and ultrastructure. Methods Adipose stromal cells were obtained and amplified in vitro. Then make use of chemical induction to induce them. Observed ADSC and differentiation of cells in morphology and ultrastructure under inverted microscope and transmission electron microscopy. Immunocytochemistry to detection of Nestin, Neuron Specific Endolase( NSE) ,and glial fibrillary acidic protein(GFAP) expression in cells. Used Real-time PCR to detection of Nestin,GFAP gene mRNA expression before and after induction in ADSC. To observe the morphology and ultrastructure of the cells prior to and after induction under microscope and electron microscope. Results The morphology of ADSC was similar to fibroblasts ,and could be amplified stability within 10 passages in vitro. Some of the cells induced display a typical astrocyte-like cells in ultrastructure. Followed neuronal induction,astrocyte-like cells began to stain brightly for CFAP, Nestin. GFAP stained in both the cytoplasm and nuclei of astrocyte-like cells, but Nestin only stained in the cytoplasm. The peak positive expression rate within 14d following neuronal induction. The rate of positive expression cells was( 14.4 ± 3. 6) % for Nestin, (87. 3 ± 5. 3 ) % for GFAP. Then two kinds of protein expression remained the similar rate. The average relative concentration of GFAP and Nestin gene mRNA have significant statistical difference between ADSC and differented cells analyzed by Real-time PCR (P<0.05).The peak concentration of GFAP was within 20 d after induction,and GFAP was within 14 d after induction. Conclusion In the cytoplasm of adult adipose-derived cells possess Nestin genetic material,which is the marker of neural stem cell. The differential astrocyte-like cells have the typical morphology, ultrastructure and GFAP phenotype of mature astrocytes.

Objective To reseach the time point of the highest percentage of neural precursor cells derived from adipose stromal cells (ADSCs) in vitro, and to observe the ultrastructure features of neural precursor cells. Methods Used the β-mercaptoethanol to induce ADSCs to differentiate into neural precursor cells and neuron-like cells. The morphology of the uninductedcells and inducted cells were observed with inverted phase contrast microscope. The expression of nestin which was the marker of neural precursor cell in each group was detected using immunofluorescence staining method. The ultrastructural feature of cells which was induced for 3 hours were observed. Results The highest ratio of positive expression of nestin was 3 hours following induction,with the ratio ( 86.25 ± 4.82) %. There were many protuberance on the cell membrane under transmission electron microscopy.There were plenty of organelles in the neural precursor cells. The neural precursor cells had a large size nucleus,large nucleoplasmic index, much extended chromatin,and less condensed chromatin. The nucleus had double-layer nuclear envelope, more nuclear pore on the nuclear envelope. Conclusion The time point of the highest percentage of neural precursor cells derived from ADSCs is 3 hours,and the ultrastructral feature of induced neural precursor cells confirm that cells at this time point are in a state of split active period.

Animals ; Bufo bufo ; Caspase 3 ; genetics ; metabolism ; Female ; Immunohistochemistry ; Male ; Proto-Oncogene Proteins c-bcl-2 ; genetics ; metabolism ; Proto-Oncogene Proteins c-kit ; genetics ; metabolism ; Spinal Cord ; metabolism ; Spinal Cord Injuries ; metabolism ; bcl-2-Associated X Protein ; genetics ; metabolism