OBJECTIVE:To prepare rapid-disintegrating oral tablets of indometacin and establish a method for its quality control.METHODS:The formula of the oral tablets was optimized by orthogonal test with the formula quality of CMS-Na(A),L-HPC(B),MCC(C),manniton(D) as factors,and the disintegrating time as index.The content of indometacin was determined by ultraviolet spectrophotometry.RESULTS:The optimal formula of the tablets was the following,A∶B∶C∶D=5∶5∶50∶5.The preparation was white in color and its quality was up to the related standard specified in Chinese Pharmacopeia (2005 edition) in identification.The linear range of indometacin was 2.08～41.6 ?g?mL-1 (r=0.999 9).The average recovery was 99.3%.The intra-day RSD=0.53% and inter-day RSD=0.74%.CONCLUSION:The preparation is simple and feasible in preparation process,and controllable in quality.

OBJECTIVE:To investigate the inhibitory effect of ligustrazine on the formation of macrophage-derived foam cells and its possible mechanism. METHODS:Human acute mononuclear cells (THP-1) were incubated with 160 nmol/L phorbol ester (PMA) for 24 h to differentiate into macrophages;and the macrophages were incubated with oxidized low-density lipoprotein (ox-LDL)culture solution containing 80 mg/L for 24 h to differentiate into macrophage-derived foam cells. And then the cells were randomly divided into blank control group(ox-LDL),model(AngⅡ)group,positive control(valsartan)group,and ligustrazine low,medium and high concentration groups(the mass concentration were 0.025,0.05 and 0.1 g/L). After all cells were respective-ly incubated with 80 mg/L ox-LDL culture solution for 48 h,oil red O staining was adopted to observe the transformation rate of foam cells,enzyme chemical method was used to determine the content of cholesterol,and real-time quantitative polymerase chain (RT-PCR)and Western blot were conducted to detect expression levels of Acyl-coenzyme A cholesterol acyltransferase-1(ACAT-1) mRNA and its protein. RESULTS:Compared with blank control group,the transformation rate of foam cells and content of choles-terol in model group were increased,and the expression levels of ACAT-1 mRNA and its protein were obviously strengthened,with significant differences(P<0.01 or P<0.05). Compared with model group,the transformation rate of foam cells and content of cho-lesterol in positive control group(valsartan)and ligustrazine low,medium and high concentration groups were decreased,and the expression levels of ACAT-1 mRNA and its protein were obviously weakened,with significant differences (P<0.01 or P<0.05). CONCLUSIONS:Ligustrazine can inhibit the macrophages differentiating into foam cells,by a mechanism that may be related to inhibiting expression of ACAT-1,and reducing content of cholesterol to reduce formation of foam cells.

OBJECTIVE To discuss the hospital infection management in infectious disease ward,to improve the ward quality of hospital infection,and to prevent and control infection inside hospital.METHODS By the plan,do,check and action(PDCA) circle method to collect information,analyze cause,establish plans,and organize to put into practice and check management.RESULTS Making the control quality of hospital infection improved obviously,various index signs attain the Hospital infection management norm and The disinfection technique norm issued by Ministry of Health and infection managment standard requirement for third class grade A hospitals.CONCLUSIONS Inducting PDCA circle method and step by step to insist on it is a good method to improve the control quality of hospital infection.

Acupuncture Therapy ; Adult ; Female ; Humans ; Lumbosacral Region ; injuries ; Male ; Middle Aged ; Sprains and Strains ; therapy ; Young Adult

Cell Line, Tumor ; Cell Proliferation ; drug effects ; Flow Cytometry ; methods ; Humans ; Point Mutation ; Stomach Neoplasms ; genetics ; pathology ; Tumor Suppressor Protein p53 ; genetics ; pharmacology

China ; epidemiology ; Humans ; Incidence ; Occupational Diseases ; epidemiology

Objective:To explore the counselor verbal response modes of psychic hot-line counseling and its characteristics.Methods:Through a qualitative study with category analysis method,8 pieces of audio recordings were collected,in each of which the entire duration was between 30 minutes to 50 minutes.And all of these record-ings were transcribed word by word.Meaningful 1202 pieces were deeply analyzed.Results:There were 5 core counselor verbal response types in hot-line counseling,greeting response,set-up response,listening response,follow-up response,and affecting response,all of which had 24 concept categories.All of the online counselors used the 5 types of verbal response.The listening response and follow-up response were the highest frequent types.Greeting response and set-up response were used mostly in the initial stage of counseling.Listening response,and follow-up response were used mostly in the stage of describing problem.Affecting response was sharply increased in the stage of analyzing problem.Greeting response and set-up response were used mostly in last stage of counseling.Conclu-sion:It suggests that there are 5 core counselor verbal response types and 24 concept categories in hot-line counse-ling.The listening response and follow-up response are the highest frequent types.Different types of verbal re-sponse modes used by counselor in different stages due to different intention.

OBJECTIVE:To explore the effect of rehabilitation training on restenosis following stent implantation in patients with coronary atherosclerotic heart disease METHODS:A computer.based online search of VIP database was performed to search articles regarding restenosis following stent implantation and rehabilitation treatment for patients with coronary atherosclerotic heart disease,published between January 2000 and October 2009,with key words"coronary artery,stent implantation,intracoronary restenosis,rehabilitation exercise".The data were collected,and the references of each article were reviewed A total of 20 articles were included.RESULTS:Intracoronary stent has become an effective treatment for coronary atherosclerotic heart disease,and intracoronary restenosis has been focused in clinical studies.Prophase treatment following stent implantation is very important for coronary atherosclerotic heart disease.Rehabilitation exercise in combination with antiplatelet anticoagulatiOn and lipid treatment can reconstruct coronary arterial blood transport and significantly reduce incidence of restenosis and thrombus.CONCLUSION:Regular rehabilitation exercise following stent implantation can ameliorate degree of vascular restenosis.

Community-acquired methicillin-resistant Staphylococcus aureus ( CA-MRSA ) has been increasingly frequently isolated from patients worldwide, and is an emerging threat to public health.CA-MRSA strains differ from hospital-acquired MRSA strains in their epidemiologies, clinical features and genetic backgrounds, and the predominant CA-MRSA strains vary between geographic settings.This paper reviews literatures on the definition, epidemiology, clinical manifestations, treatment and virulent factors of CA-MRSA or CA-MRSA infection, and points out that we should further promote studies on epidemiology, molecular genetic properties of CA-MRSA and clinical management of CA-MRSA infection.

Objective To screen the influencing factors related to prolonged mechanical ventilation (PMV). Methods Series of parameters of 154 elderly patients with pneumonia or COPD exacerbation were collected before using ventilator, after weaning of ventilator or at the 21st day of ventilating, respectively. Results By single factor analysis, PMV related to not only age, lying on bed, white blood cell, neutrophil, P (A-a)O 2, BUN, Cr, upper digestive tract bleeding, heart rate(HR), and blood pressure(BP) before using ventilator, but also related to heart function and consciousness after ventilator weaning or ventilating for 21 days. By multiple factor analysis, age, P (A-a) O 2 , heart function when ventilating for 21 days were related to PMV. Age≥82.0, P (A-a)O 2 ≥95.0 mm Hg, heart function≥grade 3 were high risk factors of PMV. Furthermore, it was found that the accuracy rate for meeting with those three parameters among 8 elderly patients with PMV was 87.5%. Conclusions Age, P (A-a)O 2 and heart function when ventilating for 21 days might be the independent factors of PMV.