Authors have examined burned patient's plasma for detecting and estimating titres of endotoxin with micro-limulus test.It was shown that low and high titres of endo-loxin were usually found in burned palients infected with gram-negative bacteria,especially Ps.aeruginosa, and with high body temperature.In patients suffering from septi-semia caused by gram-negative bacteria were usually found higher titres of endotoxin, which would endanger patients lives. Patients who were dying with a higher litre of endotoxin had the symptoms of endotoxic shock, such as a fall of body temperature and blood pressure.The micro-limulus test is a rapid, sensitive, reliable and reagent-saving in vitro method for quantitative estimation of endotoxin. It is much useful for sndotoxaemia diagnosis and helps to expedite treatment and prognosis.

ObjectiveTo research the efficiency of literature-art-therapy (LAT) using in the phrase of rehabilitation in the inpatients with schizophrenia.Methods120 inpatients were randomly divided into two groups, the trial and the control, 60 patients respectively. The trial group was treated by LAT for 3 to 18 months, while both groups were routinely treated by neuroleptic. IPROS was used in assessment at the start and the end. ResultsThe degree of functional deficiency in trial group had significant decreased. The length of time used with rehabilitation therapy had correlated directly with improvement of social function. Conclusions LAT as an efficient way of rehabilitation therapy, had played a huge role in recovery of inpatients with schizophrenia.

Angioplasty, Balloon, Coronary ; methods ; Coronary Artery Bypass ; methods ; Coronary Vessels ; surgery ; Humans

Objective To investigate the relationship between sarcoplasmic reticulum Ca2+modulation proteins and postresuscitation myocardial dysfunction. Methods Thirty-eight SPF male Sprague-Dawley (SD) rats were randomly divided into control group(n=12)and cardiac arrest(CA)group(n=26). CA was induced by intravenous bolus of potassium chloride(40μg/g),and cardiopulmonary resuscitation(CPR)was conducted 8 minutes later. No CA was induced in control group except catheter placement for monitoring cardiopulmonary parameters after anesthesia. Invasive hemodynamic parameters were monitored for 1 hour after CPR. Echocardiogram was performed to evaluate cardiac function. Myocardial samples were harvested 5 minutes and 1 hour after restoration of spontaneous circulation (ROSC),and sarcoplasmic reticulum Ca2+ ATPase (SERCA2a),phosphorylated phospholamban (p-PLB) and rynodine receptor(RyR)were determined by Western Blot. Results ROSC rate of CA group was 92.3%(24/26),and mean recovery time was (68 ±39)seconds. Cardiac function was significantly impaired in CA group at 1 hour after resuscitation, and ejection fraction, fraction shortening (FS), the maximal rate of left ventricular pressure increase/decline (±dp/dt max)were significantly decreased compared with those in control group 〔ejection fraction:0.548±0.060 vs. 0.809±0.043,F=71.692,P=0.000;FS:(34.4±4.4)%vs. (46.0±3.5)%,F=55.443,P=0.000;+dp/dt max(mmHg/s):4 718±743 vs. 7 098±394,P<0.01;-dp/dt max(mmHg/s):-3 824±612 vs.-6 187±473,P<0.01〕. Compared with control group,the expression levels of p-PLB (gray value)was significantly decreased at 5 minutes and 60 minutes(5 minutes:0.64±0.15 vs. 1.29±0.13,P<0.01;60 minutes:0.95±0.08 vs. 1.30±0.09,P<0.05)after resuscitation in CA group,while the level of sarcoplasmic SERCA2a(gray value)and RyR (gray value)showed no significant differences(SERCA2a 5 minutes:1.01±0.18 vs. 1.24±0.07,60 minutes:1.03± 0.14 vs. 1.25 ±0.06;RyR 5 minutes:0.96 ±0.13 vs. 0.97 ±0.13,60 minutes:0.88 ±0.14 vs. 0.99 ±0.11,all P>0.05). Conclusions The impairment of the p-PLB is closely related to postresuscitation myocardial dysfunction.

Objective To discuss MRI scan in the diagnosis and clinic-pathology of ovarian cystoid-material lesion.Methods We retrospectively analyzed 41 patients with clinic pathologically-proved in 2005~2007,and analyzed US,MR scan and Gd-DTPA in the diagnosis of ovarian cystoid-material lesion.Results The clinical date of the 87 patients were completed.After operation,41 of them were confirmed by pathology to be cystiform or parenchymal process,with age 16~76,average 56,of which,21 cases,were tumour-like lesions occupying 51.2%,18 cases were timorous lesions,occupying 30.2%,13 cases weer ovarian cystadenoma,occupying 30.2%,and 2case ueve other disense,occupying 4.65%.Conclusion The benign often present as ovarian cystoid-material lesion.MRI scan can show the inner structure of ovary lesion.

Objective The effect of hepatic partial resection for hepatic trauma.Methods Thirty-Six patients with hepatic trauma were operated on the bases of litertures,we elucidated the indication and method of operation and prevention of operative complications .Resuts Three cases died,a death rate of 8 33%,and a cure rate of 91 8%.Conclusion Hepatic partial resection is an effective method for treatment of hepatic trauma.

Objective:To study the feasibility and reliability of using phosphorylated H2AX(?H2AX)as a predictor for sensitivity of hepatic carcinoma cell HepG2.215 to chemotherapy agents: etoposide, doxorubicin, mitomycin, and cisplatin. Methods: HepG2.215 cells were exposed to etoposide, doxorubicin, mitomycin or cisplatin of 1, 2, 4 and 20 concentration index (CI). Untreated HepG2.215 cells were taken as control. The proportion of HepG2.215 cells expressing ?H2AX was measured by flow cytometry, the number of ?H2AX foci in HepG2.215 cells was measured by immunocytochemistry, and cell proliferation was measured by MTT. The correlation between the number of ?H2AX foci and the percentage of HepG2.215 cells expressing ?H2AX in HepG2.215 cells was analyzed; the correlation of CI with the percentage of HepG2.215 cells expressing ?H2AX or ?H2AX foci and inhibitory rate of cell proliferation was analyzed; and the correlation of inhibitory rate of cell praliferation with the percentage of HepG2.215 cells expressing ?H2AX or ?H2AX foci was also analyzed. Results: There was a positive correlation between the number of ?H2AX foci and the percentage of HepG2.215 cells expressing ?H2AX in HepG2.215 cells after treatment with the above 4 agents (all P

Objective To examine the morphological changes in the cervical intervertebral foramen during axial rotation of cervical spine and explore its clinical significance for the diagnosis and treatment of cervical degenerative disease. Methods Ten healthy volunteers (5 male, 5 female) were subjected to transectional scan by multislice spiral computed tomography of cervical spine from the upper edge of C 3 vertebra to the lower edge of C 7 vertebra. On the 45 degree oblique reconstructions of C 3～4, C 4～5, C 5～6 and C 6～7 intervertebral foramina, four parameters were measured, including foraminal height, foraminal width (both superior and inferior), and cross-sectional area of the foramen. Statistic analyses were performed to examine the morphological changes in the cervical intervertebral foramen. Results It was found that the foraminal cross-sectional area was decreased on the side of rotation,and increased on the opposite side of rotation. Significant correlation was revealed between the variation rate of the C 3～4 intervertebral foramen cross-sectional area and the rotational degree between C 3 and C 4. Conclusion Morphologic changes were found in the cervical intervertebral foramen due to axial rotation. The correlation between the cross-sectional foraminal areas with the degrees of rotation of the vertebrae was statistically significant. The results of the test have the clinical significance on the prevention, diagnosis and treatment of the cervical degenerative diseases, especially of the radiculopathy.

Objective To investigate and analyze the relative factors in elderly men with type 2 diabetes mellitus(T2DM)complicated with osteoporosis(OP),thus to provide guidance for prevention of OP complicated with T2DM in elderly men.Methods Dual X -ray absorptiometry determination of bone mineral density (BMD)of 67 cases of elderly men with diabetes,men were divided into OP group (n =32),NOP group (n =35).Then,we compared differences in body mass index(BMI),diabetes duration,fasting plasma glucose (FPG),glycosylated hemo-globin(HbA1c),patients'age,fasting insulin(FINS),insulin sensitivity index(ISI),quantitative of urinary albumin (ALb),quantitative of urinary protein in 24 hours and alkaliphosphatase level(ALP),low density lipoprotein choles-terin(LDL -C),triglyceride(TG),and made correlation analysis.Results Compared with those in NOP group,pa-tients in OP group had older age,longer disease course,smaller baric index,lower BMD,higher ALP,lower FINS,low-er ISI,higher HbA1c,higher quantitative of urinary protein in 24 hours and urinary albumin,higher LDL -C,which were significantly different (P <0.05);BMD of elderly men with T2DM was negatively correlated to patients'age, disease course,HbA1c,quantitative of urinary protein in 24 hours,ALb,ALP and LDL -C,but positively correlated to BMI,FINS and ISI.Conclusion Many factors,such as elderly,low body weight,long duration of the disease,high level of blood sugar,insulin dysfunction,low insulin sensitivity,high level of blood suger,insulin dysfunction,low insulin sensitivity,high serum alkalinephosphatase,high LDL -C and diabetic nephropathy,contribute to osteoporosis in elderly men with T2DM.

China ; Heart Defects, Congenital ; therapy ; Humans