Objective: To investigate the development of public health human resource of the Centers for Disease Control and Prevention (CDCs) in Zhejiang Province from 2016 to 2020, so as to put forward the strategies for the talent team construction of CDCs. Methods: The general information of personnel of CDCs in Zhejiang Province from 2016 to 2020 was collected through China Information System for Disease Control and Prevention. A questionnaire survey and a qualitative interview were conducted to collect the data of staffing, talent training, salary and employee turnover, and to analyze the development of the talent team of CDCs. Results: By 2020, there were 105 CDCs, including 1 provincial, 11 prefectural and 93 county-level CDCs, and 5 277 staff on-the-job. From 2016 to 2020, the number of staff per 10 000 permanent residents in the province, prefecture and county levels were 0.062-0.070, 0.170-0.188 and 0.585-0.604, respectively, which did not meet the standard for the personnel establishment in Zhejiang CDCs; the proportion of the staff with bachelor degree or above increased from 90.05% to 94.25%; the proportion of the health professionals decreased from 82.91% to 80.01%; the proportion of the staff with senior professional titles increased from 35.20% to 45.25%. The results of the qualitative interview indicated a lack of health professionals and high-end talents, a gap between the approved staffing and post requirements, as well as demands for a stable team and higher pay. Conclusion From 2016 to 2020, the structure of educational background, major and professional title among the on-the-job staff of CDCs in Zhejiang Province has been improving; however, the total allocation of human resource is insufficient, and the stability and treatment of staff need to be further improved.

OBJECTIVETo evaluate the association between chemical exposure, DNA methylation status and gene-environment interactions in the development of childhood acute leukemia (AL).

METHODSFrom January 1st 2009 to December 31st 2010, an exploratory case-control study was conducted on childhood AL among children who were less than 15 years of age in Shanghai, China. A total of 131 patients with newly diagnosed AL were recruited from 3 Shanghai children hospitals. The controls selected from the same hospital were healthy children who attended the physical check-up held by the department of Children's Healthcare, or who visited the clinic of developmental pediatrics or orthopedics (excluding blood diseases and malignant tumors). 140 controls matched with cases in gender and age were included in this study. Chemical exposure were investigated by questionnaires, methylation specific polymerase chain reaction (MSP) was adopted to analyze the methylation or deletion status of 8 genes, and gene-environment interactions were analyzed by relative excess risk of interaction (RERI), attributable proportion of interaction (API) and synergy index (S).

RESULTSThere were 131 and 140 subjects in case and control group, who were aged (6.9 ± 3.8) and (6.9 ± 3.9) years old (t = 0.01, P = 0.911), respectively. After adjusting age and other potential confounding factors, chemical substances' exposure of children/mother/father were all significantly higher in cases than that in controls (Children: OR = 3.90, 95% CI: 1.69-9.02; Mother: OR = 2.71, 95% CI: 1.12-6.52; Father: OR = 1.91, 95% CI: 1.05-3.47). For the 8 genes analyzed, the methylation status of DAPK and PTEN and P73 in case group were significantly higher than that in control group (cases: 3.1% (4 cases), 16.0% (21 cases), 7.6% (10 cases); controls: 0.7% (1 case), 2.9% (4 cases), 0.7% (1 case); χ²: 7.11, 16.90, 11.38; P value: 0.029, 0.000, 0.003). The methylation status of P16 in case group was significantly lower than that in control group (cases: 3.8% (5 cases); controls: 8.6% (12 cases), χ² = 10.33, P = 0.007). The interactions of children chemical substances' exposure and 3 genes' (PTEN, P16 and P73) methylation status were probably existed after adjusted for confounding factors (PTEN: RERI = -7.01, API = -2.14, S = 0.24; P16: RERI = 4.08, API = 0.53, S = 2.59; P73: RERI = 4.32, API = 0.48, S = 2.19), we also found the potential interaction between maternal chemical substances' exposure and PTEN, P16 gene methylation status (PTEN: RERI = -1.30, API = -0.38, S = 0.65; P16: RERI = 1.70, API = 0.38, S = 1.97).

CONCLUSIONThe study suggested the strong combined effects of chemical substances exposure of children and abnormal methylation status were risk factors of childhood AL, and there existed different interaction between them, which may indicate the important role in the pathogenesis process of childhood AL.

Acute Disease ; Case-Control Studies ; Child ; Child, Preschool ; China ; DNA Methylation ; Environmental Exposure ; Female ; Gene-Environment Interaction ; Humans ; Leukemia ; epidemiology ; Maternal Exposure ; Polymerase Chain Reaction ; Risk Factors

OBJECTIVETo investigate the association between children's and their parents' lifestyles, household environmental exposures and risk of childhood acute leukemia (AL).

METHODSA 1:2 matched case-control study of childhood AL was conducted in Shanghai between April 2011 and January 2014. The study enrolled 66 cases aged < 15, diagnosed with AL and 132 controls matched by age, gender and residence. All of the controls had no hematological diseases or previous history of malignancy. Children who had been adopted and had congenital genetic syndromes such as Down's syndrome or a positive HIV test result were not eligible as either cases or controls. Information was obtained from standardized face-to-face interviews of their parents/guardians with detailed questions on demographic characteristics, lifestyle, and household environment. Conditional logistic regression models were used to analyze effecting factors of childhood AL, odds ratios (OR) and their 95% confidence intervals (CI) were calculated.

RESULTSAmong 198 cases, 66 cases were aged (5.0 ± 3.7) years old, and 132 controls were aged (6.0 ± 3.8) years old (t = 0.48, P = 0.523). The paternal drink frequencies of cases and controls were 57.6% (38/66), and 31.1% (41/132), respectively (χ² = 4.91, P = 0.027). And the frequencies of household insecticides usage in the last year in the two groups were 78.8% (52/66), and 65.2% (86/132) (χ² = 3.87, P = 0.049). Chemical exposure during childhood (OR = 4.76, 95% CI: 1.34-16.89), maternal exposure to chemicals (OR = 4.51, 95% CI: 1.65-12.33), household insecticides use during 0-3 years of child (OR = 2.90, 95% CI: 1.31-6.39), and renovating after their children's birth (OR = 3.12, 95% CI: 1.26-7.74) were associated with an increased risk of childhood AL and these differences between the cases and the controls have statistical significance. Besides, we found that frequent contaction with other children during 0-3 years old (OR = 0.32, 95% CI: 0.15-0.69) and ventilation during sleeping in summer (OR = 0.43, 95% CI: 0.18-0.98) were associated with a decreased risk of childhood AL.

CONCLUSIONOur results support the association between children's and their parents' lifestyles, household environmental exposures and childhood AL.

Acute Disease ; Case-Control Studies ; Child ; Child, Preschool ; China ; Environmental Exposure ; Female ; Humans ; Insecticides ; Leukemia ; epidemiology ; Life Style ; Logistic Models ; Maternal Exposure ; Neoplasms ; Odds Ratio ; Parents ; Risk Factors

Objective To explore the core acupoints and compatibility rules of acupuncture and moxibustion for epilepsy by using complex network method.Methods A prescription database was es-tablished through inclusion and exclusion criteria for searching literatures for databases from China Na-tional Knowledge Infrastructure,VIP,Wanfang,Web of Science,EMBASE,and Pubmed.SPSS Modeler software was used to analyze the frequency and correlation of acupoints,and Gephi0.10.1 software was used to establish a complex network model to explore the core acupoints and acupoint se-lection rules of prescriptions for epilepsy.Results Ultimately,144 valid literatures were included,199 prescriptions were extracted,involving 102 acupoints.Baihui acupoint had the highest frequency of use,specific acupoints were mainly Five-shu acupoint,the Eight Meridian Intersection acupoint,and the Back-shu acupoint.In selection of meridians,most acupoints were selected from governor me-ridian.Association rule analysis showed that Baihui-Taichong had the highest level of support and con-fidence.The analysis of complex network topology showed that 36 acupoints such as Baihui,Dazhui,Yaoqi and Fenglong were the core acupoints in the treatment of epilepsy by acupuncture and moxibus-tion.The analysis of acupoint communities revealed three major acupoint groups including governor meridian passing through treatment group,far and near matching acupoint group of the four limbs and head,and differentiation group of Zang-fu and body fluid for epilepsy treatment.Conclusion Acu-point compatibility of epilepsy by acupuncture and moxibustion should be mainly based on principle of the governor meridian combined with the differentiation of viscera and body fluid,and attention should be paid to distal-proximal point association.

Objective To explore the core acupoints and compatibility rules of acupuncture and moxibustion for epilepsy by using complex network method.Methods A prescription database was es-tablished through inclusion and exclusion criteria for searching literatures for databases from China Na-tional Knowledge Infrastructure,VIP,Wanfang,Web of Science,EMBASE,and Pubmed.SPSS Modeler software was used to analyze the frequency and correlation of acupoints,and Gephi0.10.1 software was used to establish a complex network model to explore the core acupoints and acupoint se-lection rules of prescriptions for epilepsy.Results Ultimately,144 valid literatures were included,199 prescriptions were extracted,involving 102 acupoints.Baihui acupoint had the highest frequency of use,specific acupoints were mainly Five-shu acupoint,the Eight Meridian Intersection acupoint,and the Back-shu acupoint.In selection of meridians,most acupoints were selected from governor me-ridian.Association rule analysis showed that Baihui-Taichong had the highest level of support and con-fidence.The analysis of complex network topology showed that 36 acupoints such as Baihui,Dazhui,Yaoqi and Fenglong were the core acupoints in the treatment of epilepsy by acupuncture and moxibus-tion.The analysis of acupoint communities revealed three major acupoint groups including governor meridian passing through treatment group,far and near matching acupoint group of the four limbs and head,and differentiation group of Zang-fu and body fluid for epilepsy treatment.Conclusion Acu-point compatibility of epilepsy by acupuncture and moxibustion should be mainly based on principle of the governor meridian combined with the differentiation of viscera and body fluid,and attention should be paid to distal-proximal point association.

Objective To investigate the association between children's and their parents' lifestyles, household environmental exposures and risk of childhood acute leukemia (AL) . Methods A 1∶2 matched case-control study of childhood AL was conducted in Shanghai between April 2011 and January 2014. The study enrolled 66 cases aged<15, diagnosed with AL and 132 controls matched by age, gender and residence. All of the controls had no hematological diseases or previous history of malignancy. Children who had been adopted and had congenital genetic syndromes such as Down's syndrome or a positive HIV test result were not eligible as either cases or controls. Information was obtained from standardized face-to-face interviews of their parents/guardians with detailed questions on demographic characteristics, lifestyle, and household environment. Conditional logistic regression models were used to analyze effecting factors of childhood AL, odds ratios (OR) and their 95%confidence intervals (CI) were calculated . Results Among 198 cases, 66 cases were aged (5.0±3.7) years old, and 132 controls were aged (6.0±3.8)years old (t=0.48, P=
0.523). The paternal drink frequencies of cases and controls were 57.6% (38/66), and 31.1% (41/132), respectively (χ2=4.91, P=0.027). And the frequencies of household insecticides usage in the last year in the two groups were 78.8% (52/66), and 65.2% (86/132) (χ2=3.87, P=0.049). Chemical exposure during childhood (OR=4.76, 95%CI:1.34-16.89), maternal exposure to chemicals (OR=4.51, 95%CI:1.65-12.33), household insecticides use during 0-3 years of child (OR=2.90, 95%CI: 1.31-6.39), and renovating after their children's birth (OR=3.12, 95%CI:1.26-7.74) were associated with an increased risk of childhood AL and these differences between the cases and the controls have statistical significance. Besides, we found that frequent contaction with other children during 0-3 years old (OR=0.32, 95%CI:0.15-0.69) and ventilation during sleeping in summer (OR=0.43, 95%CI:0.18-0.98) were associated with a decreased risk of childhood AL. Conclusion Our results support the association between children's and their parents' lifestyles, household environmental exposures and childhood AL.

Objective To evaluate the association between chemical exposure, DNA methylation status and gene-environment interactions in the development of childhood acute leukemia (AL). Methods From January 1st 2009 to December 31st 2010,an exploratory case-control study was conducted on childhood AL among children who were less than 15 years of age in Shanghai, China. A total of 131 patients with newly diagnosed AL were recruited from 3 Shanghai children hospitals. The controls selected from the same hospital were healthy children who attended the physical check-up held by the department of Children's Healthcare, or who visited the clinic of developmental pediatrics or orthopedics (excluding blood
diseases and malignant tumors). 140 controls matched with cases in gender and age were included in this study. Chemical exposure were investigated by questionnaires, methylation specific polymerase chain reaction (MSP) was adopted to analyze the methylation or deletion status of 8 genes, and gene-environment interactions were analyzed by relative excess risk of interaction (RERI), attributable proportion of interaction (API) and synergy index (S). Results There were 131 and 140 subjects in case and control group,who were aged(6.9 ± 3.8)and(6.9 ± 3.9)years old(t=0.01,P=0.911),respectively. After adjusting age and other potential confounding factors, chemical substances' exposure of children/mother/father were all significantly higher in cases than that in controls (Children: OR=3.90, 95%CI:1.69-9.02; Mother: OR=2.71, 95%CI:1.12-6.52;Father:OR=1.91, 95%CI:1.05-3.47). For the 8 genes analyzed, the methylation status of DAPK and PTEN and P73 in case group were significantly higher than that in control group (cases:3.1%(4 cases), 16.0%(21 cases),7.6%(10 cases);controls:0.7%(1 case),2.9%(4 cases),0.7%(1 case);χ2:7.11,16.90,11.38;P value:0.029, 0.000, 0.003). The methylation status of P16 in case group was significantly lower than that in control group (cases: 3.8%(5 cases);controls: 8.6%(12 cases), χ2=10.33, P=0.007). The interactions of children chemical substances' exposure and 3 genes' (PTEN, P16 and P73) methylation status were probably existed after adjusted for confounding factors (PTEN:RERI=-7.01,API=-2.14,S=0.24;P16:RERI=4.08,API=0.53,S=2.59;P73:RERI=4.32,API=0.48,S=2.19), we also found the potential interaction between maternal chemical substances' exposure and PTEN,P16 gene methylation status(PTEN:RERI=-1.30,API=-0.38,S=0.65;P16:RERI=1.70,API=0.38,S=1.97). Conclusion The study suggested the strong combined effects of chemical substances exposure of children and abnormal methylation status were risk factors of childhood AL, and there existed different interaction between them, which may indicate the important role in the pathogenesis process of childhood AL.

Objective To investigate the association between children's and their parents' lifestyles, household environmental exposures and risk of childhood acute leukemia (AL) . Methods A 1∶2 matched case-control study of childhood AL was conducted in Shanghai between April 2011 and January 2014. The study enrolled 66 cases aged<15, diagnosed with AL and 132 controls matched by age, gender and residence. All of the controls had no hematological diseases or previous history of malignancy. Children who had been adopted and had congenital genetic syndromes such as Down's syndrome or a positive HIV test result were not eligible as either cases or controls. Information was obtained from standardized face-to-face interviews of their parents/guardians with detailed questions on demographic characteristics, lifestyle, and household environment. Conditional logistic regression models were used to analyze effecting factors of childhood AL, odds ratios (OR) and their 95%confidence intervals (CI) were calculated . Results Among 198 cases, 66 cases were aged (5.0±3.7) years old, and 132 controls were aged (6.0±3.8)years old (t=0.48, P=
0.523). The paternal drink frequencies of cases and controls were 57.6% (38/66), and 31.1% (41/132), respectively (χ2=4.91, P=0.027). And the frequencies of household insecticides usage in the last year in the two groups were 78.8% (52/66), and 65.2% (86/132) (χ2=3.87, P=0.049). Chemical exposure during childhood (OR=4.76, 95%CI:1.34-16.89), maternal exposure to chemicals (OR=4.51, 95%CI:1.65-12.33), household insecticides use during 0-3 years of child (OR=2.90, 95%CI: 1.31-6.39), and renovating after their children's birth (OR=3.12, 95%CI:1.26-7.74) were associated with an increased risk of childhood AL and these differences between the cases and the controls have statistical significance. Besides, we found that frequent contaction with other children during 0-3 years old (OR=0.32, 95%CI:0.15-0.69) and ventilation during sleeping in summer (OR=0.43, 95%CI:0.18-0.98) were associated with a decreased risk of childhood AL. Conclusion Our results support the association between children's and their parents' lifestyles, household environmental exposures and childhood AL.

Objective To evaluate the association between chemical exposure, DNA methylation status and gene-environment interactions in the development of childhood acute leukemia (AL). Methods From January 1st 2009 to December 31st 2010,an exploratory case-control study was conducted on childhood AL among children who were less than 15 years of age in Shanghai, China. A total of 131 patients with newly diagnosed AL were recruited from 3 Shanghai children hospitals. The controls selected from the same hospital were healthy children who attended the physical check-up held by the department of Children's Healthcare, or who visited the clinic of developmental pediatrics or orthopedics (excluding blood
diseases and malignant tumors). 140 controls matched with cases in gender and age were included in this study. Chemical exposure were investigated by questionnaires, methylation specific polymerase chain reaction (MSP) was adopted to analyze the methylation or deletion status of 8 genes, and gene-environment interactions were analyzed by relative excess risk of interaction (RERI), attributable proportion of interaction (API) and synergy index (S). Results There were 131 and 140 subjects in case and control group,who were aged(6.9 ± 3.8)and(6.9 ± 3.9)years old(t=0.01,P=0.911),respectively. After adjusting age and other potential confounding factors, chemical substances' exposure of children/mother/father were all significantly higher in cases than that in controls (Children: OR=3.90, 95%CI:1.69-9.02; Mother: OR=2.71, 95%CI:1.12-6.52;Father:OR=1.91, 95%CI:1.05-3.47). For the 8 genes analyzed, the methylation status of DAPK and PTEN and P73 in case group were significantly higher than that in control group (cases:3.1%(4 cases), 16.0%(21 cases),7.6%(10 cases);controls:0.7%(1 case),2.9%(4 cases),0.7%(1 case);χ2:7.11,16.90,11.38;P value:0.029, 0.000, 0.003). The methylation status of P16 in case group was significantly lower than that in control group (cases: 3.8%(5 cases);controls: 8.6%(12 cases), χ2=10.33, P=0.007). The interactions of children chemical substances' exposure and 3 genes' (PTEN, P16 and P73) methylation status were probably existed after adjusted for confounding factors (PTEN:RERI=-7.01,API=-2.14,S=0.24;P16:RERI=4.08,API=0.53,S=2.59;P73:RERI=4.32,API=0.48,S=2.19), we also found the potential interaction between maternal chemical substances' exposure and PTEN,P16 gene methylation status(PTEN:RERI=-1.30,API=-0.38,S=0.65;P16:RERI=1.70,API=0.38,S=1.97). Conclusion The study suggested the strong combined effects of chemical substances exposure of children and abnormal methylation status were risk factors of childhood AL, and there existed different interaction between them, which may indicate the important role in the pathogenesis process of childhood AL.

The purpose of this study is to reveal the role of Girdin in regulating the aggregation of actin filaments by studying the relationship between PKB/Akt and Girdin. First we used Scansite software (http://scansite.mit.edu) to predict relevant target sites of PKB/Akt on mouse Girdin. To gain insight into the role of phosphorylation of Girdin by PKB/Akt, we assessed the location of phosphorylated Girdin in fertilized eggs by staining with anti-P-Girdin 1 417 Ab. We detected a distinct increase in the fluorescence signal of F-actin and P-Girdin 1 417 after microinjection of Akt WT and myr-Akt. The addition of myr-Akt induced phosphorylation of Girdin in mouse fertilized eggs. In addition, siRNA-mediated Akt-knockdown blocked phosphorylation of Girdin. The distribution of actin filaments was obviously scattered. These results strongly suggest that PKB/Akt could directly phosphorylate Girdin on Ser1 417 and promote its function in mouse fertilized eggs.
Actins ; physiology ; Animals ; Mice ; Microfilament Proteins ; physiology ; Phosphorylation ; Proto-Oncogene Proteins c-akt ; physiology ; RNA, Small Interfering ; Vesicular Transport Proteins ; physiology ; Zygote