Objective:To investigate the relationship of the expression of IL-2 and IL-4 in breast cancer cells with chemosensitivity. Methods:Surgical samples of breast cancer,diagnosed by pathology,were obtained from The First Affiliated Hospital ofChongqing Medical University. The cancer sample cells were cultured separately in the incubator at 37℃,5%CO2 in vitro. The rate of inhibition of cancer cells by 4 kindsof anticancer drugs,which were EADM,5-Fu,NVB and DDP,were assayed by MTT method. Immunohistochemistry was used to detect the expression of IL-2 and IL-4 in the breast cancer tissues. Results:Sensitivity rates of 20 breast cancer cells in 0.1?PPC within 48h in vitro were 30%(EADM),20%(5-Fu),45%(NVB)and 25%(DDP).Respectively the rate of inhibition of EADM,5-Fu,NVB and DDP were significantly higher in the IL-2 positive cancer cells than in the IL-2 negative cancer cells. A positive correlation was found between expression of IL-2 and chemosensitivity for all the 4 anticancer drugs. The rates of inhibition of EADM and NVB were significantly lower in the IL-4 positive cancer cells than in the IL-4 negative cancer cells. A negative correlation was found between expression of IL-4 and chemosensitivity for the 2 anticancer drugs. Conclusions:The expression of IL-2 and IL-4 are related with the chemosensitivity of the breast cancer cells,and they may become one of the markers for judging the effect of chemotherapy in clinic. The combined detection of IL-2 and IL-4 may be taken as an index of the selection of anticancer drugs.

Acupuncture Points ; Adolescent ; Adult ; Child ; Combined Modality Therapy ; Electroacupuncture ; Exercise Therapy ; Female ; Humans ; Male ; Middle Aged ; Patella ; injuries ; Rheumatic Diseases ; therapy ; Young Adult

Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Neck Injuries ; therapy ; Young Adult

Acupuncture Therapy ; Fingers ; blood supply ; Gangrene ; therapy ; Humans ; Male ; Middle Aged

Objective To evaluate the value of transvaginal two-dimensional ultrasound(2D-TVS)and transvaginal three-dimensional ultrasound (3D-TVS) in the diagnosis of intrauterine lesions with abnormal uterine bleeding.Methods Intrauterine lesions for 171 patients underwent 2D-TVS followed by 3D-TVS in the same session.Within 10 days of the sonographic session,each patient underwent pathologic histology examination after hysteroscopy examination or surgery therapy,which was considered the reference standard.For each of the two imaging methods,sensitivity,specificity,and accuracy were calculated.Results The results of 2D-TVS and 3D-TVS had good agreement with pathological results in endometrial hyperplasia,endometrial polyps,uterus submucous myoma and endometrial cancer.For 2D-TVS,sensitivity was 84.5％,71.4％,86.8％,66.7％,and specificity was 86.7％,90.7％,97.0％,98.7％,respectively; For 3D-TVS,sensitivity was 91.4％,87.3％,92.1％,83.3％,and specificity was 93.8％,96.3 ％,97.7 ％,98.7 ％,respectively.The diagnose accordance rate of the 3D-TVS for intrauterine lesions was statistically higher than that of the 2D-TVS (89.5％ vs 78.9％,P ＜0.05).Conclusions The sensitivity and diagnose accordance rate of 3D-TVS was higher than that by 2D-TVS,3D-TVS plays an important role in the diagnosis and differential diagnosis of intrauterine lesions.

OBJECTIVE To discuss about the infection control management in medical posts located in disaster tents. METHODS The experience of hospital infection control in disaster tent medical posts under special conditions was summarized. RESULTS Under the difficult conditions due to strict management system of infection control and strengthening the posts management,strict disinfection to reduce the spread of the infection was prevented. There were 15877 injured victims treated in disaster tents medical posts and 80 of them were seriously injured,but no infectious disease outbreak incidents were happened,and the safety of every victim and medical staff was ensured. CONCLUSIONS Strengthening management and strictly conducting disinfection and isolation system especially in primitive conditions could prevent the onset and spread of the nosocomial infection.

Objective To study the antitussive and antiasthmatic effect of compound liquor from Tremella aurantialba. Methods The mice cough model induced by ammonia, guinea pig cough model induced by acitric acid,guinea pig asthma model induced by histamine and acetylcholine, guinea pig asthma model induced by ovalbumin were used in this experiment. Results The compound liquor from Tremella aurantialba could significantly prolong the tussive delitescence,decreas the tussive times of mice and guinea pigs;could significantly prolong the asthmatic delitescence. Conclusions The antitussive and antiasthmatic effects of compound liquor from Tremella aurantialba was obvious.

Objective To review the relationship between the expression levels of bcl-2,bax and bad gene and other biological factors of breast cancer in the growth and development of breast cancer.Methods Related literatures were summarized and reviewed.Results The expression level change of antiapoptosis gene bcl-2 was still under research and the expression levels of apoptosis gene bax and bad were down-regulated progressively in the evolution from benign breast tissue to breast cancer tissue.The expression level of bcl-2 had positive correlation with some positive factors in breast cancer such as estrogen receptor(ER) and progesterone receptor(PR),while it had negative correlation with some negative factors such as p53,EGFR,c-erbB-2 and lymph node metastasis.The levels of ER,PR and the expression level of p53 of breast cancer had no relationship with the expression level of bax.Up to now there was no report about the relationship between the expression level of bad and other biological factors of breast cancer.Conclusion The role of altered expression level of bcl-2,in the treatment and prognosis of breast cancer is still controversial,and the relationship between the expression of bad and the prognosis of breast cancer is still unknown,but expression level of bax is correlated positively with the prognosis of breast cancer.Research on these genes can provide us some new index to evaluate the prognosis of breast cancer and new ideas on treatment of breast cancer including gene therapy.

Prostate cancer is a common malignancy of the male reproductive system.This paper reviews the epidemiology of prostate cancer in China and around the world, and discusses the risk factors of prostate cancer.

Objective To investigate the value of three-dimensional color power angio(3D-CPA)in the prenatal diagnosis of pernicious placenta previa (PPP ) with placenta increta.Methods The ultrasonographic findings of PPP with placenta increta diagnosed in our hospital by 2D-CDFI and 3D-CPA were reviewed and compared with pathologic histology examination.For each of the two imaging methods, sensitivity,specificity,positive predictive value and prenatal diagnose accordance rate were summarized. Results For 3D-CPA,sensitivity,specificity and positive predictive value were 91.1%,88.9% and 93.3%, respectively.The results of 3D-CPA were higher than those in 2D-CDFI (sensitivity 76.4%,specificity 66.7%,positive predictive value 81.5% ),and were statistically significant (P <0.05 ).The diagnose accordance rate of 3D-CPA was statistically higher than that of 2D-CDFI (90.4% vs 73.0%;P <0.05). Conclusions 3D-CPA can display abnormal vascular reconstruction between the placenta and uterine wall from various angles,and is more accurate than 2D-CDFI.3D-CPA plays an important role in the prenatal diagnosis of PPP with placenta increta.