Objective To explore the influences of Jinganning decoction on the content of CYP2E1 and cytokine IL-6,TNF in rats models of alcohdic hepatic injury.Methos 72 male Wistar clean level rats were randomly divided into 6 groups:blank control group,Jiuganning drug toxicity test group,alcoholic hepatic disease model group,Yiganling treatment group,Jiuganning low dose group,and Jiuganning high dose group.Blank control group was treated with physiological saline;Jiuganning drug toxicity test group was treated with Jiuganning decoction to observe toxicity and side effects.Jiuganning low and high dose treatment group were treated with 17.5 g/kg and 23.5 g/kg crude drug.Yiganning treatment group were treated with 0.206 g/kg crude drug.After 4 weeks'treatment,the content of TNF,IL-6,MDA as well as the activity of CYP2E1 and SOD were measured.Results Compared with the alcoholic hepatic disease model group,the content of IL-6(F=46.281,9.101),TNF and the activity (F=62.537)of CYP2E1 of all treatment group were significantly improved(P＜0.01).The content of SOD(F=19.210)in the rats serum of all treatment group was significantly increased while the MDA(F=34.952)was decreased.All the indexes in the treatment group had significant differences with the alcoholic hepatic disease and model group(P＜0.01).Conclusions The mechanism of Jiuganning decoction can effectively reduce the content of CYP2E1 and IL-6,TNF-a,with the changes of cell physiopathology and the influences of relevant enzymes,reveals the functional routines of the decoction treating hepatic injury:anti-lipid peroxidative,adjusting cytokine's activity,protecting hepatocyte,palliating the hepatocyte inflammation,and easing fibration.

A tumor suppressor gene at 10q23.3, designated PTEN, encoding a dual specificity phosphatase with lipid and protein phosphatase activity, has been shown to play an important role in the pathogenesis of a variety of human cancers and sporadic tumor. In this paper, we reviewed the construction and function of PTEN, and the mechanism of activation in cancer especially in thyroid carcinoma.

Animals ; Child ; China ; Dairy Products ; Diet ; Health Promotion ; methods ; Health Status ; Humans ; Nutritional Status ; Nutritive Value

Objective To study the ankle-brachial index of the diabetic gangrene. Methods ABI of the sixty cases with type 2 diabetes were measured. The results were divided into four groups: normal, gently abnormal moderate abnormal severely abnormal group. Results 30 cases were normal, occupying 50% (P＞0.05), gently and moderate abnormal attained 5 cases (8.33%) and 4 cases (6.67%) respectively, and the rests 21cases were severely abnormal, occupying 35% (P＞0.05).Conclusion The normal ABI was not always indicating type 2 diabetes without the gangrene or tendency.

Objective:To explore the risk factors its predictive value of delayed traumatic intracranial hematoma (DTICH) . Methods:Clinical data of 95 patients with acute craniocerebral trauma were collected .All patients were divided into DTICH group (n=62) and no recurrent hematoma control group (n=33) .Clinical data were compared between two groups ,Lo‐gistic regression model was used to analyze risk factors .Receiver operator characteristic curve (ROC) was used to assess pre‐dictive value of relative risk factors .Results:Compared with no recurrent hematoma group ,there were significant rise in age [(46.72 ± 6.45) years vs .(52.18 ± 7.13) years] ,hematoma volume [(51.21 ± 11.25)ml vs .(56.89 ± 12.37)ml] , level of fasting blood glucose [(8.13 ± 1.55) mmol/L vs .(10.62 ± 1.73) mmol/L] ,activated partial thromboplastin time [APTT ,(25.27 ± 6.19)s vs .(27.35 ± 7.45)s] and thrombin time [TT ,(17.53 ± 5.14)s vs .(21.26 ± 7.31)s] ,significant increase in percentages of basal cistern compression (24.2% vs .46.8% ) ,brain contusion (42.4% vs .69.4% ) ,bone flap removal (45.5% vs .67.7% ) and positive Babinski sign (39.4% vs .61.3% ) , and significant reductions in GCS score [(9.46 ± 3.29) scores vs .(7.63 ± 3.07) scores] ,systolic blood pressure [(146.57 ± 18.23)mmHg vs .(132.18 ± 17.18) mmHg] and timing of surgery [(12.74 ± 4.39)h vs .(5.47 ± 2.16)h] in DTICH group ,P<0.05 or <0.01;Logistic regres‐sion analysis indicated that coagulation function (APTT ,TT) ,skull fracture and timing of surgery were risk factors for post‐operative delayed hematoma(OR=4.076~5.430 ,P<0.05 or <0.01) ,their area under curve of ROC were 0.826 ,0.748 and 0.661 respectively .Conclusion:Coagulation function ,skull fracture and timing of surgery are risk factors and possess considerable predictive value for postoperative delayed craniocerebral hematoma in patients with craniocerebral trauma .

Objective To explore the application and the clinical curative effect of Ilizarov technique in infected tibia defect with soft tissue defect .Methods It was case series retrospective study .Fifteen patients (6 women ,9 men;age 23-54 ,average 38) with infected tibia defect combined soft tissue defect between March 2008 and March 2012 were treated with Ilizarov external fixa‐tion .The average length tibia defect was 7 .7 cm(3 .0 -21 .0 cm) ,The average area of soft tissue defect was 7 .5 cm2 (3 .6 -18 .4 cm2 ) .Ilizarov external fixation was applied in the operation .Results All 15 patients were followed up and the mean time was 16 .2 months (range ,8-36 months) .The average time of the bony healing was 12 .3 months (range ,7 -28 months) .The docking site was bony healing after rapid compression in 4 cases .The docking site was not bony healing in 2 cases .The docking site achieved bony healing by accordion technique in one case ,and bone grafting in the other case .The skin and hypodermis were embedded in the docking site in 9 cases .The skin and hypodermis were displaced in 3 cases ,and bone grafted at the same time in the other 6 cases . The soft tissue defect gradually become small and healed at last in the 14cases .One case with large soft tissue defect was cured by skin grafting .According to Paley′s evaluation criteria ,12 cases were excellent ,2 good ,1 moderate ,giving a good to excellent rate of 93 .3% .Conclusion Ilizarov technique is a safe and effective method to treat infected tibia defect with soft tissue defect which can completely debride infected lesions ,repair the soft tissue defect ,cure bone nonunion and restore the limb length .

Hypoxia and localized acidosis,increased interstitial pressure,perturbations in structure and function of the extracellular matrix,the production of growth factors and protein hydrolase,angiogenesis,and co-optation of the immune response are all microenvironment that promote tumor survival and metastasis.The application of animal models is critical to the understanding of the pathophysiology in these processes.This review will show the advances of animal models in the study of tumor microenvironment.

Objective To understand the infection caused by multidrug-resistant organisms(MDROs)in a neurological in-tensive care unit (Neuro-ICU),and evaluate the effect of comprehensive intervention measures.Methods Targeted monito-ring on MDROs among patients who hospitalized in a Neuro-ICU for >48 hours between March and December 2011 was implemented,comprehensive intervention measures were taken,MDRO infection before and after intervention was com-pared.Results A total of 932 patients were monitored,72 (7.73%)developed MDRO healthcare-associated infection (HAI);the top five MDROs were Acinetobacter baumannii ,Klebsiella pneumoniae ,Pseudomonas aeruginosa ,Staphylo-coccus aureus ,and Escherichia coli .The main infection site of MDRO infection was lower respiratory tract,followed by urinary tract and bloodstream.Detection rates of MDROs before and after intervention were 11.70%(n=55)and 3.68%(n=17)respectively(χ2 =16.675,P <0.001).Conclusion Patients in Neuro-ICU are prone to develop MDRO infection, comprehensive intervention measures can reduce the incidence of HAI.

Bisphenol A(BPA)is an environmental endocrine disruptor(EDC)that has been shown to be harmful in laboratory animal studies.However,there are relatively few epidemiological studies to prove the relationship between BPA and health effects in human.This review summarizes the epidemiological studies about BPA and children health,analyze the association between BPA exposure and health outcomes,including neonatal birth weight,productive abnormal,neurobehavioral development,wheeze or asthma,early puberty,obesity.

Objective We aimed to evaluate the correlation of oral care with chlorhexidine on the incidence of ventilator-associated pneumonia.Methods The PubMed,Medline,Cochrane Library,CNKI,VIP,Wanfang database until December 2013 about the clinical randomized controlled trials (RCT) of the oral care with chlorhexidine on the incidence of ventilator-associated pneumonia were searched.Software for data analysis was RevMan5.2.Results Finally 15 RCTs involving 2 615 patients were included.In 15 articles the experimental group used chlorhexidine solution or gel for oral nursing involving 1 319 cases,the control group with a total of 1 296 cases,used placebo for oral nursing involving 459 cases,837 cas-es used 0.9％ saline solution or sterile water.Results of Meta-analysis indicated that there were 11.30％ (149/1 319)patients with VAP in the experimental group,20.29％ (263/1 296) patients in the control group.The VAP incidence between two groups had significantly statistical difference [OR=0.48,95％ confidence intervals (CI) was from 0.34 to 0.67,P ＜ 0.01].The incidence of VAP in oral care with chlorhexidine solution group (no tooth brushing) was 12.23％ (80/654),in the control group the incidence of VAP was 25.44％ (172/676),there was significantly statistical difference between two groups [OR=0.36,95％CI was 0.27 to 0.50,P ＜ 0.01].The incidence of VAP in oral care with chlorhexidine gel group (no tooth brushing) was 10.47％ (27/258),in the control group the incidence of VAP was 15.36％ (41/267),no statistical difference showed by comparison of two groups [OR =0.65,95％ CI was 0.39 to 1.09,P ＞ 0.05].The incidence of VAP in oral care with chlorhexidine solution group (tooth brushing) was 10.32％(42/407),in the control group the incidence of VAP was 14.16％ (50/353),there was significantly statistical difference between two groups [OR=0.55,95％ CI 0.34 to 0.90,P＜0.05].Conclusions Oral care with chlorhexidine solution (tooth brush-ing or no tooth brushing) can effectively reduce the incidence of ventilator associated pneumonia.There was no sufficient evidence to indicate that using chlorhexidine gel (no tooth brushing) for oral care can reduce the incidence of ventilator associated pneumonia.