Objective To explore the influences of Jinganning decoction on the content of CYP2E1 and cytokine IL-6,TNF in rats models of alcohdic hepatic injury.Methos 72 male Wistar clean level rats were randomly divided into 6 groups:blank control group,Jiuganning drug toxicity test group,alcoholic hepatic disease model group,Yiganling treatment group,Jiuganning low dose group,and Jiuganning high dose group.Blank control group was treated with physiological saline;Jiuganning drug toxicity test group was treated with Jiuganning decoction to observe toxicity and side effects.Jiuganning low and high dose treatment group were treated with 17.5 g/kg and 23.5 g/kg crude drug.Yiganning treatment group were treated with 0.206 g/kg crude drug.After 4 weeks'treatment,the content of TNF,IL-6,MDA as well as the activity of CYP2E1 and SOD were measured.Results Compared with the alcoholic hepatic disease model group,the content of IL-6(F=46.281,9.101),TNF and the activity (F=62.537)of CYP2E1 of all treatment group were significantly improved(P＜0.01).The content of SOD(F=19.210)in the rats serum of all treatment group was significantly increased while the MDA(F=34.952)was decreased.All the indexes in the treatment group had significant differences with the alcoholic hepatic disease and model group(P＜0.01).Conclusions The mechanism of Jiuganning decoction can effectively reduce the content of CYP2E1 and IL-6,TNF-a,with the changes of cell physiopathology and the influences of relevant enzymes,reveals the functional routines of the decoction treating hepatic injury:anti-lipid peroxidative,adjusting cytokine's activity,protecting hepatocyte,palliating the hepatocyte inflammation,and easing fibration.

Animals ; Child ; China ; Dairy Products ; Diet ; Health Promotion ; methods ; Health Status ; Humans ; Nutritional Status ; Nutritive Value

A tumor suppressor gene at 10q23.3, designated PTEN, encoding a dual specificity phosphatase with lipid and protein phosphatase activity, has been shown to play an important role in the pathogenesis of a variety of human cancers and sporadic tumor. In this paper, we reviewed the construction and function of PTEN, and the mechanism of activation in cancer especially in thyroid carcinoma.

Objective To study the ankle-brachial index of the diabetic gangrene. Methods ABI of the sixty cases with type 2 diabetes were measured. The results were divided into four groups: normal, gently abnormal moderate abnormal severely abnormal group. Results 30 cases were normal, occupying 50% (P＞0.05), gently and moderate abnormal attained 5 cases (8.33%) and 4 cases (6.67%) respectively, and the rests 21cases were severely abnormal, occupying 35% (P＞0.05).Conclusion The normal ABI was not always indicating type 2 diabetes without the gangrene or tendency.

OBJECTIVE: Differential cognition and evaluation of the scientific merit of free radical-induced aging theory in the explanation of aging mechanism.DATA SOURCES: Literatures about free radical-induced aging theory were searched in Medline and Embase by computer from April 1976 to October 2004 with searching words of "free radical, aging, dialectical" and the language of the articles was limited in English, the subjects were the elderly and aging animal.STUDY SELECTION: To select literatures about free radical-induced aging theory for preliminary examines. Quality evaluation mainly examined the authenticity of the data, whether the survey design was rigorous, whether the intervention process was strict, and whether statistical management was reasonable.DATA EXTRACTION: A total of 30 articles about the merit of free radical-induced aging theory in the explanation of aging mechanism were searched, of which 18 experiments were in accorded with the inclusive criteria. In the 12 excluded experiments, 7 articles were the repetitions of same study, and 5 articles were Meta analytic study.DATA SYNTHESIS: Comparative analysis was conducted in the experimental results of aging essence and the prolongation of maximum duration of life explained by free radical-induced aging theory.CONCLUSION: Free radical-induced aging theory has an important biomedical academic merit, but it does not successfully explain the fundamental reason of agingand the essence of aging process.

Although che mical manage ment progra ms have been in place in several countries for more than 30 years they continue to be chal enged by factors such as data quality and quantity,greater de mand on regulatory staff and re-sources,an evolution in hazard and exposure assess ment methods,and continued debate about manage ment progra ms that are risk-based (hazard and exposure)or hazard-based.Countries developing their own che mical manage ment pro-gra ms often wish to learn fro m the experience of others,and thereby have progra ms that either reduce or eli minate so me of the proble ms associated with existing progra ms.However,often even new progra ms face the sa me or new proble ms that have been associated with managing che micals.In this paper we su mmarize so me of the ele ments of existing pro-gra ms in the United States and Europe,the developing progra m in China,and the chal enges associated with the evolution of hazard,exposure and risk assess ment methods.We conclude by suggesting areas where further research may be use-ful for i mproving che mical manage ment progra ms.

Objective To explore the clinical effect of bifidobacterium lactobacillus triple living bacterium tablet in the prevention and treatment of pediatric antibiotic associated diarrhea(AAD).Methods 300 children with respiratory tract infection were selected,firstly they were divided into group A(1 month-lyear old) 140 cases and group B(1-3years old) 160 cases according to the age;secondly,they were randomly divided into observation group (group A:70 cases and group B:80 cases) and control group 150 cases (group A:70 cases and group B:80 cases)within the A,B groups.Control group was given antibiotics to treat respiratory tract infection,and received conventional treatment after diarrhea appeared.Observation group was given bifidobacterium lactobacillus triple living bacterium tablet combined with antibiotics,continued to receive this drug on the basis of conventional treatment after diarrhea appeared.The incidence rate of diarrhea,total effective rate,duration of diarrhea were observed.Results The incidence rate of diarrhea of observation group was obviously lower than the control group,diarrhea treatment overall effective rate obviously higher than control group,the duration of diarrhea significantly shorter than the control group,with significant difference(P ＜ 0.05) ;The incidence rate of diarrhea of observation group in group B was obviously lower than the observation group in group A,with significant difference (P ＜ 0.05).Conclusion The clinical effect of bifidobacterium lactobacillus triple living bacterium tablet in the prevention and treatment of pediatric AAD is significant,especially for 1-3 years old children,it is worth clinical promotion.

Objective To explore the clinical efficacy of combined anterior and posterior operation in the treatment of cervical spondylotic myelopathy.Methods 50 patients with cervical spondylotic myelopathy,in accordance with anterior,posterior surgery and combined anterior and posterior operation three different surgical methods,were divided into the treatment group A(21 cases),group B(16 cases) and group C(13 cases).The surgical efficacy was analyzed.Results Before treatment,the JOA score among the three groups had no significant difference [Cgroup (9.3 ± 2.3) points,B group (9.4 ± 1.4) points,A group (9.2 ± 2.2) points].After treatment,the JOA score in group C (14.5 ± 3.4) points was significantly higher than that of group A (12.6 ± 1.2) points (t =3.09,P ＜0.05) and group B (11.3 ± 1.4) points (t =4.62,P ＜ 0.05).The excellent and good rate of C group was 76.9％,which was significantly higher than that in group A 43.8％ (x2 =3.84,P ＜ 0.05) and group B 42.8％ (x2 =4.11,P ＜ 0.05).Conclusion Combined anterior and posterior operation in the treatment of cervical spondylotic myelopathy has better recovery of spinal cord function,complete decompression,significant effect,less complications,etc.,it is an effective,safe and reliable treatment.

During the past 30 years, Alzheimer's disease (AD) drug R&D aiming at a variety of potential targets has undergone tremendous setback although a large amount of resources have been invested by pharmaceutical companies, governments, academic institutions and venture capitals globally. There is no doubt that uneasy early diagnosis, unsatisfying therapeutic strategies and genetic polymorphism of AD patients bringing variety of responses to the drugs contribute to the failure of AD drug therapy, but the most important reason might be that people have insufficient understanding of the very complex diseases including AD. Continuing to make efforts in the previous way to find AD drugs might not be a good way. Development of systems biomedicine opens an avenue to understand the disease networks and pursue multi-target drugs R&D based on systems pharmacology theory; neural stem cell transplantation, and neurogenesis affected by small molecules might bring new hope for AD therapy; discovery of new biomarkers of AD will help the study of the pathogenesis and diagnosis of the disease and finally the discovery of new types of AD drugs.

Objective To investigate the effects of ACEI combined with beta-blocker on cardiac function in patients with chronic heart failure (CHF)and explore its action mechanisms.Methods 60 CHF patients were enrolled in this study.The patients were randomly divided into the two groups:conventional treatment group(group A,n =30),ACEI combined with beta-blocker treatment group (group B,n =30).The LVEF,LVEDD,MMP-9,and hsCRP were recorded after treatment.The expression of MMP-9 was measured by ELISA.The level of serum hs-CRP was measured by latex enhanced immunoturbidimetric assay.Results The improvement of LVEF and LVEDD in B group [(63.21 ± 6.09) ％,(58.42 ± 5.73)mm] were better than those in A group [(41.13 ± 3.67) ％,(66.24 ± 4.24) mm] (t =7.62,6.54,P ＜ 0.01).The expressions of MMP-9 and hs-CRP in group B [(1.31 ± 0.24) ng/ml,(6.34 ± 0.58) mg/L] were significantly lower than those in A group[(2.23 ±0.39) ng/mL,(8.31 ±0.74) mg/L] (t =6.82,7.03,P ＜0.01).The total effective rate in A group was 36.7％,which was significantly lower than 73.3％ in B group(x2 =6.90,P ＜ 0.05).Conclusion The cardiac function was improved by ACEI combined with beta-blocker treatment in CHF patients.The downregulation of MMP-9 and hs-CRP may play a key role in the effective ACEI combined with beta-blocker treatment.