Objective To evaluate the effect of local infiltration anesthesia with different concentrations of ropivacaine on the healing of incision in rats.Methods Seventy-five adult male Sprague-Dawley rats,weighing 220-250 g,were randomly divided into 5 groups (n =15 each):natural healing group (group C),normal saline group (group NS),0.20％ ropivacaine group (group R0.20),0.50％ ropivacaine group (group R0.50) and 0.75％ ropivacaine group (group R075).0.20％,0.05％ and 0.75％ ropivacaine 1.0 ml/kg were injected subcutaneously 10 min before incision in R0.20,R0.50 and R0.75 groups,respectively,while the equal volume of normal saline was given instead in group NS.Five rats in each group were chosen for recording the healing time.Ten rats were taken in each group for recording the length of the incision 72 h after operation.The skin tissues around the incision were removed for determination of fibroblast counts under light microscope,IL-1β content (by ELISA) and the expression of collagen (by immunohistochemisty) and IL-1β mRNA (by RT-PCR).Results Compared with Cgroup,the healing time and length of the incision were significantly shortened,the fibroblast counts and expression of collagen were increased in R0.20 group,the healing time and length of the incision were shortened,the fibroblast counts and expression of collagen were increased,and the expression of IL-1β mRN A and IL-1β content were decreased in R0.50 group,and the healing time and length of the incision were prolonged,the fibroblast counts and expression of collagen were decreased,and the expression of IL-1β mRNA and IL-1β content were decreased in R0.75 group (P ＜ 0.05).Compared with R0.20 group,the healing time and length of the incision were significantly shortened,the fibroblast counts and expression of collagen were increased,and the expression of IL-1β mRNA and IL-1β content were decreased in group R0.50,the healing time and length of the incision were significantly prolonged,the fibroblast counts and expression of collagen were decreased,and the expression of IL-1β mRNA and IL-1β content were decreased in group R0.75 (P ＜ 0.05).Compared with R0.50 group,the healing time and length of the incision were significantly prolonged,the fibroblast counts and expression of collagen were decreased,and the expression of IL-1β mRNA and IL-1β content were decreased in group R0.75 group (P ＜ 0.05).Conclusion Local infiltration anesthesia with 0.20％ and 0.50％ ropivacaine can promote the healing of incision in rats,0.50％ ropivacaine provides better efficacy than 0.20％ ropivacaine,and 0.75 ％ ropivacaine can inhibit the healing process.

Humans ; Medicine, Chinese Traditional ; Syndrome ; Temperature

BACKGROUND: Low power laser irradiation has positive effects on fracture healing, including shortening the time of bone union and enhancement of bone formation. OBJECTIVE: To evaluate the effects of different wave lengths in low power laser on eedy fracture healing. DESIGN, TIME AND SETTING: Randomized, controlled, animal experiment. The study was performed at the Laser Center of Dankook University Medical Center between May 2005 and May 2006. MATERIALS: A total of 60 female English Hartley guinea pigs, 9 weeks old, weighing (300～30) g, were used for this experiment. They were randomly divided into three groups control, 632, and 830 nm groups (n=20). METHODS: The right femoral middle shaft fractures were made with a bone cutter and fixed with intramedullary nails (1.4 mm diameter K-wire). The frequencies of 632 nm and 830 nm semi-conductor laser machines were used. The laser irradiations were started 48 hours after operation vertically at the fracture sites and were applied every two days, at 100 J/cm<'2> of the same irradiation doses and 2.52 cm<'2> of the same irradiation areas in different irradiation groups. Finally, 46 guinea pigs survived, including 14 in control group, 16 in 632 nm group and 16 in 830 nm group. MAIN OUTCOME MEASURES: Guinea pigs were sacdficed at 3 and 6 weeks separately. The bone healing was assessed by the Modified Zorlu Scoring System, including gross, radiologic and histologic examinations. RESULTS: In the gross and radiologic findings, both 632 nm and 830 nm irradiation groups shown a significantly greater rate of callus formation at postoperatively 3 weeks compared to the control group (P< 0.01). Quantity of callus formation in 830 nm group was more than 632 nm group, but there was no significant difference (P > 0.05). The result of histological findings showed a significant increase of osteoblastic proliferation in two irradiation groups than the control group at 3 weeks postoperatively (P < 0.01). Enhancement of osteoblastic proliferation was more obvious in 830 nm group than in 632 nm group at 3 and 6 weeks, but there was no significant difference (P > 0.05).CONCLUSION: Both 632 nm and 830 nm wave lengths are considered to be optimal irradiation wave lengths for eady fracture healing. Moreover, 830 nm irradiation is better than 632 nm irradiation for eady fracture healing.

Acupuncture Therapy ; Anal Canal ; pathology ; Female ; Humans ; Intervertebral Disc Displacement ; complications ; Middle Aged ; Pain ; etiology ; Rectal Diseases ; etiology ; pathology ; therapy

The paper analyzes the application advantages of information technology in medical informatics education.Takes Shanghai Sipo Polytechnic College as an example, it elaborates the specific application of information technology in medical informatics education, including immersion education based on 3D virtual reality technology, two-way video teaching and other aspects.It also gives solutions on current existing problems.

Objective To analyze the causes of surgical errors and complications of lumbar degenerative diseases treated by transforaminal endoscopic spine system(TESSYS),and explore the treatment and prevention measures.Methods From July 2012 to January 2016,the data of 660 patients with lumbar degenerative diseases who treated with TESSYS were analyzed retrospectively,of which 528 patients were lumbar disc herniation with 192 cases of single segment(L4/L5) and 336 cases of multi-segment.Lumbar spinal stenosis in 132 cases,were lateral recess and nerve root canal stenosis.Surgical errors and complications were recorded,and analyzed the reasons and the treatment and preventive measures.Results Intraoperative errors:1 case of sterile film with the dilating catheter went into the surgical area;1 case with wire fracture,and stump retention in vivo;for discography staining,the proportion of mixed solution was not enough,the leakage of excess concentration of methylene blue caused caudal nerve injury in 3 cases;expansion of the intervertebral foramen,the drilling part of the articular process was too deep and too close to the medial wall of the articular process,which caused cutting injury of nerve fiber sheath in 2 cases;after the rupture of the dura mater,the rinse solution was perfused,caused bloating,elevated intracranial pressure,elevated blood pressure and other symptoms of spinal cord hypertension in 3 cases;low dose of local anesthetic into the blood caused toxic reaction in 2 cases;total spinal cord anesthesia with paravertebral nerve root block in 1 case.Postoperative complications:3 cases of nerve root edema;2 cases of free nucleus pulposus tissue;2 cases of local hematoma led to nerve root compression;1 case of intervertebral space infection and 3 cases of local skin neuritis in the puncture area.Conclusion In the process of intervertebral foramen surgery,there are some deficiency on instruments or operating skill.Therefore,rigorous treatment of each link in the perioperative period,is the guarantee of successful surgery.

By describing the humanities educational status in medical universities,the authors show the comparatively lower level and the more simple and backward humanities education ways in China and point out that administrators should perform the humanities education all round,not only from the curriculum offering but also from the class contents and the campus humanities environments to make the humanities education penetrate in every part of medical education.

Objective To compare the clinical effects of fiber post‐retained and metal post‐retained restorations .Methods Two hundred teeth after root canal therapy from 176 patients were divided into 2 groups randomly and each group consisted of 100 teeth .In the experimental group ,teeth were restored with fiber post‐resin core and porcelain crown ,where cast metal‐core and porcelain crown were used in the control group .The teeth were clinically examined regularly for 2 years .The data were analyzed with Chi‐square method .Results At the end of the 2‐year follow‐up ,6 fiber posts dislocated and 2 teeth showed gingival inflammation in the experimental group ,with a clinical success rate of 92% ,the loss rate of 6% .5 gingival inflammation cases ,2 periapical inflammation cases and 7 cases of root fracture occurred in the metal post group ,and the clinical success rate was 85% ,root discount rate of 7% .There was statistical significance between the two groups (P< 0 .05) .Conclusion The loss rate ogf fiber post‐retained restoration is higher than the metal post‐retained restorations ,but the root discount rate is low ,incidence of periodontal and periapical tissue similar in the two groups can be routinely used clinically instead of metal post‐retained restoration .

Objective:To establish a method for the determination of specnuezhenide in traditional decoction and granules of Zish-enwufa formula, and compare the content between them. Methods:HPLC was used for the determination. The samples were analyzed on a Waters Symmetry C18 column (150 mm × 3. 9 mm,5 μm) with acetonitrile-water (16 ∶84) as the mobile phase. The flow rate was 1. 0 ml·min-1 and the detection wavelength was 224nm. Results: A good linearity of specnuezhenide was within the range of 0.179 2-3.316 μg(r=0.999 9),and the average recovery of berberine hydrochloride was 101.65% with RSD of 1.49%(n=6). Conclusion:The determination method is simple, accurate and sensitive with good reproducibility,which can be used for the determi-nation of specnuezhenide in the preparation. The content of specnuezhenide in the traditional decoction and granules of Zishenwufa for-mula is similar.

AIM:To study the effect of mistletoe lectins on cell proliferation and apoptosis in HT-29 human colon cancer cell.METHODS:The effect of mistletoe lectins on cell viability in HT-29 cell was evaluated by MTT assay.Cell apoptosis was observed by DNA ladder analysis and Tunel.RESULTS:Mistletoe lectins(eg.,1-4 mg/L) inhibited cell growth in a dose and time dependent way in HT-29 cells.HT-29 cells apoptosis can be induced when exposed to mistletoe lectins(eg.,1-4 mg/L).CONCLUSION:Mistletoe lectins can inhibits proliferation and induces apoptosis in HT-29 cells in vitro.