The paper introduces reference work in the new age from its modes, contents, characteristics and developing strategies,including establishing characteristic resources, improving quality of librarians, carrying out network consultant and information navigation, establishing collaborative digital reference, in order to provide reference for primary hospital libraries.

Objective To establish the quality standard for Yuyuan Mingmu Granula. Methods Radix Et Rhizoma Notoginseng,Fructus Schisanrae Chinesis,Flos Scophorae and Fructus Forsythiae were identified by TLC. The content of loganin was determined by HPLC. Results Under the selected conditions,clear and specific spots were obtained. For content determination,the linear range of loganin was 0.084 ?g～0.840 ?g,r=0.999 9,the average recovery was 98.5 %(n=6)and RSD was 1.29 %. ConcLusion The method is proved to be simple and specialized with a good reproductivity and can be used to control the quality of Yuyuan Mingmu Granula.

BACKGROUND: At present, more and more people pay attention to the protection of sub-hypothermia on cerebral ischemic tissue and the effect of sub-hypothermia treatment on inflammatory reaction after cerebral ischemia-reperfusion.OBJECTIVE: To investigate the effect of sub-hypothermia on inflammatory cytokine in cerebral ischemic area of rats and protection on cerebral reperfusion injury.DESIGN: Randomized controlled study on the basis of animals.SETTLNG: People's Hospital of Hubei Province.MATERIALS: The experiment was completed at Laboratory Center of Renmin Hospital of Wuhan University from October 2004 to February 2005. Totally 50 healthy SD rats of clean grade were selected in this study.METHODS: Ten SD rats were divided randomly into normal group and sham operation group with 5 in each group. Other 40 rats were divided randomly into normal temperature cerebral ischemia group and sub-hypothermia cerebral ischemia group with 20 in each group. Rats except 5 in normal group were used to establish reversible ligation model of left middle cerebral artery (MCA) with Longa ligation method. Rats in sham operation group and normal temperature group were put in 20 ℃ room, and anus temperature was maintained at 37 ℃; rats in sub-hypothermia group were put in 4 ℃ room and anus temperature was maintained at (33.0±1.0) ℃.Rats in sub-hypothermia group were treated with sub-hypothermia on whole body, after ischemia, temperature was changed normally, and reperfusion was started 3 hours after cerebral ischemia. All modeling rats were scored with neurological defect: 0 point: none of neurological defect; 1 point: unable to unfold bilateral anterior claws; 2 points: cycling to hemiplegia side during walking; 3 points: falling to hemiplegia side; 4 points: unable to self-walk and with unconsciousness.MAIN OUTCOME MEASURES: Expression of tumor necrosis factor (TNF) andinterleukin-1 in cerebral tissue of ischemic rats, percentage of volume of cerebral infarction,and neurological functional scores.RESULTS: Fifteen rats were excluded because of intracranial hemorrhage,anesthetic accident and unqualified neurological functional scores. Other 15 rats with successful modeling were supplied randomly, and totally 50rats entered the final analysis. ① Number of TNF positive cell in immunohistochemical staining: There were no significant differences between normal group and sham operation group [(3.54±1.24, 3.71±1.50) /sight]; but numbers in sub-hypothermia group were lower than those in normal temperature group [(31.94±7.23, 69.20±9.43)/sight, F=179.16, P ＜ 0.001]. ②Numbers of interleukin-1 positive cells in immunohistochemical staining:There were not significant differences between normal group and sham operation group [(3.20±1.34, 3.89±2.08) /sight]; but numbers in sub-hypothermia group were lower than those in normal temperature group [(28.95±4.97, 55.79±7.93)/sight, F=174.95, P ＜ 0.001]. ③ Volume percentage of infarct focus: Percentage in sub-hypothermia group was lower than that in normal temperature group [(21.06±2.42)%, 30.32±2.71], F =374.87, P ＜ 0.001]. ④ Neurological functional scores: Percentage in subhypothermia group was lower than that in normal temperature group [(1.35±0.27)%, (2.04±0.34)%, F=117.17, P ＜ 0.001].CONCLUSION: ① Volume of cerebral infarction focus is lower in subhypothermia group than that in normal temperature group, this suggests that sub-hypothermia can protect ischemic neurons. ② Positive expressions of TNF and interleukin-1 are observed rarely in normal group and sham operation group, but more expression of positive cells are observed in cerebral ischemic area with 24-hour reperfusion after ischemia, this suggests that cerebral ischemia starts expression of inflammatory cytokine, evoke inflammatory cascade reaction, deteriorate cerebral ischemia-reperfusion injury. Therefore, inhibition of inflammatory cascade reaction is one of important mechanisms of protecting brain.

Progesteron could significantly inhibit the contracted effect of strips of tracheal and lung smooth muscles isolated from guinea pigs, which induced by Ach His and 5- HT. It also inhibited the contractility of phase I caused by intracellular calciumefflux in the course of 5 -HT stimulating tracheal smooth muscles and the Schultz - Dale responsiveness of sensilized of tracheal smooth muscles guinea pigs.

Objective:To study parenting style of difficultly educated children and the state of their mental health.Methods:Randomly select 120 difficultly educated children from a Training Camp in Changsha and used SCL-90 and EMBU to rate their parenting style and mental health.Results:The mental health condition of difficultly educated children was significantly worse than the norms,but the normal middle school students parental warmth was higher than the difficutly educated students.Difficultly educated children scored significantly higher in father's firm control,punishment,over interference,rejection and denial and maternal rejection and denial,firm control and punishment,but significantly lower in parental warmth.Conclusion:Difficultly educated children had got more mental problems,and their parents treat them with more negative parenting style and less positive parenting style.

Objective To study sedative hypnotic pharmacodynamics of Shui'an Capsule,and provide experimental basis for the expansion of clinical indications.Method The effects of different concentrations of Shui'an Capsule on spontaneous activity of mice,induced dormancy of the mice with subliminal and suprathreshold dose of sodium pentobarbital and resisted convulsions of mice caused by Dimenlini Hydrochloridum were observed.Result Shui'an Capsule could significantly decrease times of spontaneous motion in mice,prolong sleeping time with pentobarbital sodium of superthreshold dose,and decrease the latent period of sleep.Conclusion Shui'an Capsule produces obvious sedative hypnotic effect.

Objective To determine whether the AT, All66C gene polymorphism is associated with CHD in type 2 diabetes patients. Methods Investigating the allele frequency and the genotype distribution of the AT1 All66C gene in the group of normal control (n= 73),simple diabetes (n= 80),diabetic CHD (n=43) by means of PCR-RFLP. Results The group of diabetic CHD had a significant higher frequency of C allele of the AT1 All66C gene than normal control (X2 = 6. 02,P＜0. 05) and diabetes (X2= 5. 71,P＜0. 05). Conclusion The result indicated that there was an association between the 1166C allele of the AT1 gene polymorphism and CHD in type 2 diabetes patients.

Objective To study the distribution of Apolipoprotein E(ApoE) gene polymorphism and mtDAN*!4336 mutation in patients with sporadic Alzheimer's disease(AD),and analyse the difference of frequency of ApoE genotype from some other studies.Methods The polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) technique was used to analyze the ApoE allele genotype and mtDAN mutation of 127 AD patients and 138 healthy elderly controls.Results In AD group, we found 2.4% of the patients with 2 ApoE4 allele gene, 18.1% of the patients with 1 ApoE4 allele gene, and 79.5% of the patients without ApoE4 allele gene. However, in control group, the frequency was 0.7%, 10.1% and 89.2% respectively. There was a significant difference between the two groups( P

This paper reviews the problem and shortage of IPv4 network which was unfolded during its use in telemedicine field and describes the features and advantage of IPv6, which is the next generation protocol of Internet by comparing it to IPv4. The application of IPv6 in telemedicine field is prospected. The work should be done and the problem may be met during the change from IPv4 to IPv6 network are also discussed.

Objective To determine the effect of walking in water on patients with spinal cord injury~SCI). Methods Twenty SCI patients were recruited in this study, and these patients were randomly divided into a control group ~10 cases) and an experiment group~10 cases).The patients of the experiment group were administered aqua-walking and conventional physical therapy~PT),and the patients of control group were administered the conventional PT only. Results After 10 weeks of therapy, the muscle tone, motor index score and functional ambulation classification of experiment group were ameliorated significantly in comparison with the control group(P