Objective To evaluate the imaging quality and diagnostic value of different MR sequences for abnormalities of knee cartilage. Methods Three sequences of MR, including double echo steady state (DESS), multi echo data imagine combination (MEDIC) and true fast imaging with steady state precession (TrueFISP) were performed in 18 healthy volunteers. Signal-to-noise ratio (SNR) of knee cartilage and contrast-to-noise ratio (CNR) to surrounding tissues were measured and compared. Results SNR of cartilage was the highest in MEDIC sequence imaging, CNR of cartilage-synovia was the highest in DESS sequence imaging among three sequences (P<0.05). Conclusion Compared with the other sequences, DESS seems to be optimal in diagnosis of abnormalities in knee cartilage.

Objective:To investigate the use of antibacterial drugs during the perioperative period of a cesarean section and observe the differences before and after antibacterial drug remediation. Methods:1 569 cesarean cases from 2010 to 2012 were investigated, and the use of antibacterial drugs, drug categories, drug combinations, medical expenses, and so on was statistically analyzed with the use of the statistical software SPSS13. 0. Results:The rate of antibacterial drug usage within the 1 569 cesarean cases was 100%;for pre-operative antibacterial drug remediation, azithromycin, cefathiamidine, and ornidazole were the top three, while the top three antibacterial drugs for post-oper-ative remediation were arecefathiamidine, cefuroxime, and clindamycin. There was no significant difference between the two groups of incision infections, and the rate for combined use of drugs dropped. The analysis of drug costs is of statistical significance. Conclusion:The analysis of antibacterial drug remediation is useful for the rational use of anti-bacterial drugs and a reduction of costs.

Objective To analyze the clinical effects, as well as its advantages and disadvantages, of laparoscopic surgery for appendicitis in obese patients. Methods Clinical data of 80 obese patients receiving appendectomy, including 38 cases of open appendectomy and 42 cases of laparoscopic appendectomy, were analyzed retrospectively.The operative time, blood loss, analgesic and antibiotic use, postoperative fever, postoperative complications, length of hospital stay, and hospitalization cost were compared between the two operative methods. Results Conversions to open surgery were required in 2 patients in the laparoscopic group. There was no significant difference in operative time between the 2 groups (P >0.05).Compared with the open group, the laparoscopic group had less blood loss [(14.98 ±12.77) ml vs.(31.58 ±19.00) ml, t=-4.550, P=0.000], shorter time of postoperative antibiotic use [(2.7 ±1.0) d vs.(4.1 ±1.2) d, t=-5.470, P=0.000], less postoperative analgesics needed [5.0%(2/40) vs.26.3%(10/38),χ2 =6.802, P=0.009], less drainage [2.5% (1/40) vs.18.4% (7/38), χ2 =5.367, P=0.021], less postoperative fever [5.0%(2/40) vs.23.7%(9/38),χ2 =5.616, P=0.018], less postoperative wound healing [5.0%(2/40) vs.21.1%(8/38),χ2 =4.493, P=0.034], and shorter hospital stay [(5.9 ±3.2) d vs.(8.7 ±4.1) d, t=-3.345, P=0.001], but the higher cost of hospitalization [(7800 ±396) yuan vs.(4914 ±434) yuan, t=30.716, P=0.000]. Conclusion For obese patients with appendicitis, laparoscopic appendectomy has less surgical trauma, faster recovery, less postoperative pain, fewer complications,and shorter hospital stay, being a preferred method of treatment.

Laparoscopic surgery for upper-third gastric cancer has gradually been accepted by experienced surgeons as the mature of this technique.Different from the standardized and programmed D2 lymph node dissection in Laparoscopy-assisted Billroth Ⅰ gastrectomy,the indications and methods for laparoscopic splenic hilar lymphadectomy in the upper-third gastric cancer remains controversial.Unsolved problems include joint organs resection,appropriate surgical approach selection and variable vascular anatomy of the splenic hilum.Meanwhile,the long-term efficacy and safety of laparoscopic splenic hilar lymphadectomy for the upper-third gastric surgery need to be confirmed by evidence-based medical trials.With the advance of the theory and clinical practice,laparoscopic splenic hilar lymph node dissection will continue to progress.

OBJECTIVE: To study the absorption and transport of pachymic acid (PA) isolated from the sclerotium of Poria cocos (Schw.) Wolf. in human intestinal epithelium. METHODS: By using Caco-2 (the human colonic adenocarcinoma cell lines) cell monolayers as an intestinal epithelial cell model, the permeability of PA was studied from apical side (AP side) to basolateral side (BL side) or from BL side to AP side. The PA was measured by reversed-phase high performance liquid chromatography coupled with UV detector at maximum absorption wavelength of 210 nm. Transport parameters and apparent permeability coefficients (Papp) were then calculated and compared with those of propranolol and atenolol, which were the transcellular transport markers for high and poor permeability respectively. RESULTS: The Papp values of PA were (9.50+/-2.20) 10(-7) cm/s from AP side to BL side, and (11.30+/-5.90) 10(-7) cm/s from BL side to AP side, respectively. Under the condition of this experiment, the Papp values were 1.45x10(-5) cm/s for propranolol and 4.22x10(-7)cm/s for atenolol. CONCLUSION: PA is transported through the Caco-2 cell monolayer in a concentration-dependent manner and the transport was linear with time. The absorption in apical to basolateral direction and secretion in basolateral to apical direction were poor and their Papp values were comparable to atenolol. Besides passive diffusion of PA, ATP is partially involved in its transport.

Objective To investigate the association of the SNP in miRNA146A with genetic prediposion and earlier recurrence after resection for hepatocellular carcinoma (HCC).Methods In the casecontrol study including 173 HCC cases,DNA were exacted from cancer tissue embedded with paraffin and were amplificated by PCR,SNP was explored in gene sequence of miRNA146a (385 base pair including extron).The outcome were analyzed with genetic prediposion and clinical features.Result Only hsa-mir-146a rs2910164 was found.The genetype frequence of C/C 、G/G and C/G at rs2910164 gene locus were separately 61 (35.3％),21 (15％) and 86(49.7％) in cases.Compared to G/G genetype,C/C and C/G genetype were danger factor to onset risk of HCC (OR =3.086,95％ CI:1.289-7.390) ; C/G was danger factor to earlier recurrence after resection(OR =8.179,95％ CI:2.248-29.759).Conclusion rs2910164 may be associated with genetic prediposion and earlier recurrence after resection of HCC in Jiangxi hans

"Resolving,espulsing and tonifying" three methods are the general outline of endotherapy in surgery of TCM.The author of this article thinks that pelvic inflammation and appendagitis can be classified in internal ulcer and vusceral carbuncle,and it can be treated by "Resolving,espulsing and tonifying" three methods.As the general programme of treating female inflammatory aphoria in different periods,the three methods can avoid exfetatio and promote the probability of pregnancy.Resolving method is applied in acute period of inflammatory aphoria,active period of chronic inflammatory and chronic pelvic inflammation when abdominal mass has formed but vital qi is not deficient.The pathologic character in this period is sufficiency of vital qi and excessive of pathogenic qi.Expulsing method is applied in inflammatory aphoria patients whose pathogenic has not been cleared but the vital qi has been harmed,at this time damp heat pathogen poison remain in uterus meridians and the nvital qi has no power to resist pathogen,and whose corporeity is always week and infects pathogen poison which results in the course of diseases being repeated and prolonged.Tonifying method is applied after the resolving and espulsing methods have been used.In this period,damp heat stasis have been cleared,inflammation and inflammatory matters have been absorbed,disease has been improved and patient has had condition to be pregnant.Tonifying method can promote human generative function and improve the possibility of conception.

Objective Comparing the variability of four genes of HCMV in the population and the therapy process of single patient.Methods Using PCR to amplify the four genes and restriction enzyme to validate four fragments, These four genes are UL55,UL57,UL86 and UL122 whose coding products are glycoprotein B,single chain binding protein,main capsid protein and IE pp86 separately. Then we use AD169 to optimize the conditions of PCR and restriction enzyme. then we use these conditions to detect 200 blood specimens. Results The PCR positive rate of UL55 is 12.5% and restriction positive rate is 10.0%, which has marked difference with other 3 gene segments(P

Whole rice grain was dissected by hand into fractions of pericarp, ale-urone, scutellum, embryo and endosperm. For the convenience of having enough amount of samples for amino acids assay some of which were recom-bined and finally three parts, pericarp +aleurone layer, scutellum + embryo and endosperm, were obtained. Protein, lysine, methionine, threonine and valine contents were determined for each part. The data obtained indicated that although total weight of the first two parts accounted for only 7.27-8.43% of the whole grain, they contributed 14.5-15.8% protein, 29.0-30.5% lysine, 11.8% melhionine, 16.8% valine and 19.4% threonine, contained in the whole grain. During husking process it indicated that the lesser amounts of pericarp, aleurone, scutellum and embryo were retained and the larger percentage of protein and lysine was lost. This fact was identified either by a specially designed miller in the laboratory or a commonly used sand wheel miller in the factory. The suitable degree of milling for rice was discussed and it was proposed that 92% extration for rice rather rational.

Lysine and methionine contents were determined in proteins of 21 rice samples including 17 varieties grown in 11 provinces. Ranges of lysine and methionine contents on crude protein basis were 3.21-3.76% and 1.63-2.06% respectively. The lysine content was found to be negatively correlated with the protein content of rice, but no correlation was found in case of methionine when they were expressed on the basis of crude protein. The absolute amounts of lysine and methionine were positively correlated with protein contained in the samples. The importance of breeding rice variety with protein of high lysine content was discussed in relation to promoting the protein nutritive status in South China.