Objective To investigate the epidemic situation of brucellosis in Yingkou City of Liaoning Province,and to provide a basis for making prevention and control measures.Methods Surveillance data of human brucellosis in Yingkou City from 2005 to 2014 were collected throng the China information system for disease control and prevention.The method of retrospective data analysis was used to analyze the distributions of region,time,age,sex,and occupation distribution of brucellosis.Results A total of 348 cases were infected with brucellosis in Yingkou City from 2005 to 2014,268 males and 80 females,and the sex ratio was 3.35:1.00.The age was mainly concentrated in 35-＜ 65 years old,a total of 251 cases,accounting for 72.13％ (251/348) of the total number of cases.Farmer was the main occupation of the patients,295 cases,accounting for 84.77％ (295/348) of the total number of cases.The city's lowest incidence was in 2011,0.340/100 000,the highest in 2014,3.343/100 000.All counties (districts or cities) had reported the epidemic,Gaizhou was the highest,a total of 225 cases,accounting for 64.66％ (225/348) of the total number of cases.The onset time focused on April-September each year,249cases,accounting for 71.55％ (249/348) of the total number of cases.Conclusions The epidemic of brucellosis in Yingkou City is in a spreading trend.Health education and inter-departments cooperation are in need in order to control the spreading of the epidemic.

〔Abstract〕 The paper analyzes problems existing in the current course of medical literature retrieval and proposes suggestions for re -forming the teaching contents and methods , including the integration of multimedia teaching and online class , integration of class teaching and practical operation , and integration of multiple teaching methods such as case teaching method , etc.It aims to effectively improve the teaching quality of medical literature retrieval and realize the objectives of cultivating medical students 'information literacy .

According to WHO, breastfeeding is the best for premature babies. Breastfeeding are beneficial for health of both mothers and their premature infatns and as well as reduction of medical and health expenditure. However, the breastfeeding rate in premature infants is still unsatisfactory so far, especially in those premature infants admitted to NICU in the early life. This article review promoting and hindering factors of breastfeeding in hospitalized premature infants from the following aspects:the characteristics of premature infants, the demographic and sociological characteristics, knowledge, beliefs, and psychological status of mothers, hospital policies and support, and family support.

Objective To investigate the changes in bone age, and the correlation between the changes in structure and function of cardiovascular and bone age index in children with left to right shunt congenital heart disease (CHD) such as atrial septal defect (ASD), ventricular septal defect (VSD) and patent ductus arteriosus (PDA). Methods One hundred and thirty children diagnosed CHD had been enrolled, including 52 cases of ASD, 46 cases of VSD and 32 cases of PDA. The cardiac structure and function indicators had been detected by ultrasonic. The anteroposterior iflm of left hand and wrist had been taken. The bone age had been assessed according to“China children bone age score”atlas, and the bone age index (BAI) had been calculated. The differences of bone age among each group had been compared. The linear correlation of the cardiac structure and function indicators with BAI had been analyzed. Results The BAI was statistically different among ASD, VSD and PDA groups (P<0.05). The BAI of PDA group was higher than those of ASD and VSD groups (all P<0.05). The BAI was also statistically different among the groups with different pulmonary artery pressures (P=0.000). The BAI was higher in small defect size group than that in large defect size group (P=0.002). The defect size was negatively correlated with BAI in both ASD and VSD groups (r=-0.48, -0.54, all P<0.05), The pulmonary artery systolic pressure and pulmonary-to-systemic blood lfow ratio (QP/QS) were negatively correlated with BAI in ASD, VSD and PDA groups (r=-0.64--0.38,all P<0.05). Conclusion The bone age and BAI of children with left to right shunt CHD are signiifcantly lower than those of healthy children of the same age . The extent of bone age delay is related with PASP, defect size and shunt volume in children with left to right shunt CHD.

Mammal multidrug and toxin extrusion proteins (MATEs) play an important role in the transport of organic cations in the body. MATEs mediate the final excretion step for multiple organic cation drug used clinically and important endogenous substances. This article reviews the discovery, type, gene coding and polymorphism, body distribution, classification of substrates and inhibitors and their research method of MATEs. The article also discusses the major research significance of MATEs with examples.

ObjectiveTo observe the efficacy of electroacupuncture with different wave patterns, sparse wave, intense wave, and sparse-intense wave, on the therapeutic efficacy in treating mild cognitive disorder after ischemic stroke.MethodSixty eligible patients were randomized into a sparse wave group, an intense wave group, and a sparse-intense wave group, 20 in each group. The three groups were given electroacupuncture at Baihui (GV20) and Shenting (GV24) in addition to the basic acupuncture treatment.The Mini-mental State Examination (MMSE) and Activities of Daily Living (ADL) were adopted for evaluation before and after the intervention by the same practitioner.ResultThe total effective rate was 55.0% in the sparse wave group, 65.0% in the intense wave group, and 90.0% in the sparse-intense wave group, and thedifferenceswere statistically significant among the three groups (P<0.05); afterintervention, the MMSE score increased significantly and ADL score decreased significantly in the three groups (P<0.01), and the inter-group differences were statistically significant after intervention(P<0.01).ConclusionElectroacupuncture with sparse wave, intense wave, or sparse-intense wave all can improve mild cognitive disorder, while the general therapeutic efficacy of sparse-intense wave is higher than sparse wave and intense wave.

Objective To survey the incidence of adverse reaction induced by traditional Chinese medicine injection and provide valuable suggestion for using traditional Chinese medicine injection.Methods Clinical data of 1860 patients who were under treatment of traditional Chinese medicine injection during January 2010 to December 2014 were studied to survey adverse reaction incidence, effected system-organs and risk factors of adverse reaction.Results Comparison of incidence adverse reaction of traditional Chinese medicine injection, Qingkailing injection adverse occurrence rate reached the highest 11.76% for all types, followed by Shenmai injection and Shuanghuanglian injection.Adverse reactions involving the organ and the performance was compared, adverse reactions mainly involved the organs for the skin and accessories, circulatory system, accounting for 56.15%, the incidence of adverse reactions of the rest of the organ system was less.Not determine the treatment based on differentiation of symptoms and signs, combined with injection, ultra treatment application and distribution of liquid static for a long time all differences were statistically significant ( P <0.05), leading to the main risk factors of the occurrence of adverse reactions.Conclusion Traditional Chinese medicine injections occurred and the incidence of adverse reactions was higher, wider range, organ involvement mainly skin and its appendages and circulatory system, not dialectical treatment and rational drug use is lead to adverse risk factors, actively preventment and timely treatment to reduce the incidence of adverse reactions.

Objective To observe and analyze the expressions of Toll like receptors (TLRs)of TLR2,TLR4 in peripheral blood mononuclear cells and its correlation with the inflammatory factors levels of the elderly patients with chronic obstruc-tive pulmonary disease (COPD).Methods 80 cases of elderly patients with COPD were selected and divided into the COPD stable phase group and the AECOPD group according to their condition of diseases.43 patients and 37 patients were included into each group respectively.80 cases of healthy elderly people were selected and divided into the smoking group and the non smoking group.41 cases and 39 cases were included into each group respectively.The expression levels of TLR2,TLR4 in peripheral blood mononuclear cells and the serum levels of interleukin-6 (IL-6),interleukin-8 (IL-8),tumor necrosis factor-α(TNF-α)of the objects in all the groups were detected and compared.Results From the AECOPD group,the non smoking group to the COPD stable phase group,the expression levels of TLR2,TLR4 in peripheral blood mononuclear cells of the objects increased significantly gradually and the TLR4 expression level in peripheral blood mononuclear cells of the patients in the COPD stable phase was significantly higher than that in the smoking group (q=2.815~3.754,P <0.05).From the AECOPD group,the COPD stable phase group,the smoking group to the non smoking group,the serum IL-6,TNF-αlevels of the objects decreased significantly gradually and the serum IL-8 level of the patients in the AECOPD group was signifi-cantly higher than that of the other three group (q=5.247~8.175,P <0.05).The expression level of TLR2 in peripheral blood mononuclear cells of COPD patients was correlated with the serum IL-6 level (standardized regression coefficient=0.458),the serum IL-8 level (standardized regression coefficient=0.622)and the serum TNF-αlevel (standardized regres-sion coefficient=0.562)(P <0.05),the expression of TLR4 was correlated with the serum IL-6 level (standard the regres-sion coefficient= 0.478),the serum IL-8 level (standardized regression coefficient= 0.569)and the serum TNF-α level (standardized regression coefficient=0.352)(P <0.05).Conclusion The elderly patients with COPD showed the overex-pression state of TLRs of TLR2,TLR4 in the peripheral blood mononuclear cells.Such increases were correlated closely with the serum levels of the inflammatory factors in the patients.The overexpression of TLRs should involve in the occur-rence and the progression of COPD.

OBJECTIVE: To study the apoptosis/proliferation of Kölliker organ supporting cells and to understand the prompting apoptosis factors in vivo in the supporting cells in the Kölliker organ by changing the environment of the cultured supporting cells in the Kliker organ in vitro, via the separation, culture and purification of the supporting cells in the K6lliker organ. METHOD: A combinatorial approach of enzymatic digestion and mechanical separation was employed to isolate and culture in vitro pure Kölliker organ supporting cells. The purity was tested by flow cytometry assay. And K6lliker organ supporting cells were harvested to detect the rate and cycle of apoptosis by flow cytometry after Annexin V/PI staining, to test the cell growth curve by MTT assay, and to observe the differential expressions of the Bcl-2, Caspase-3, Caspase-8 and Caspase-9 through the Realtime PCR and Western blot. The calcium, potassium and glutamate concentrations in the culture medium of these cells in vitro were changed to detect the survival rate of cells by MTT assay. RESULT: The purity of K6lliker organ supporting cells by flow cytometry assay was 96. 56%. And these cells showed no significant difference in apoptosis, but an evident linear growth. The results of Realtime PCR and Western blot showed that the expression of Bcl-2, Caspase-3, Caspase-8 and Caspase-9 mRNA and protein in all different time points kept stable. Furthermore, the elevation of extracellular Ca2+ might contribute to decrease the cell viability of supporting cells. And K+ participated regulation of cell viability in a concentration-depending way. However, glutamate appeared to be a protective factor in high concentration. CONCLUSION There is no significant apoptosis in vitro of the supporting cells in the Kölliker organ of rats, showing a linear growth. The Ca2+ in high concentration might contribute to the apoptosis factor of these cells. However, the K+ and glutamate appear to be protective factors in high concentration.
Animals ; Animals, Newborn ; Apoptosis ; Caspase 3 ; Cell Cycle ; Cell Proliferation ; Cell Survival ; Cochlea ; cytology ; growth & development ; Flow Cytometry ; In Vitro Techniques ; Rats

Objective The aim of the study was to investigate the effects of inflammation on the biological be-haviors of the VF -MSCs and provide the theoretic basis for the repair of the vocal folds which were damaged by in-flammation .Methods The inflammatory vocal fold tissues and normal vocal fold tissues were respectively derived from the vocal cord polyp and normal tissues of the hypopharyngeal carcinoma patients .The HE staining ,masson trichrome staining and elastin van gieson (EVG) staining were performed to detect the effects of inflammation on the collagenous fiber and elastic fibers of the vocal fold lamina propria .The cell colony formation analysis and MTT cell growth curve were used to detect the effects of inflammation on the proliferation of VF -MSCs .The effects of in-flammation on the multi-directional differentiation of VF -MSCs were evaluated by inducing the VF -MSCs to dif-ferentiate into osteoblasts and lipoblasts .Results The results of masson trichrome staining and EVG staining showed that in the inflammatory vocal fold lamina propria collagen fibers became thicker and the amount of collagen fibers increased ,while elastic fibers became thinner and the amount of elastic fibers decreased .Compared with the vocal fold mesenchymal stem cell (VF-MSCs) in normal vocal folds ,VF-MSCs in inflammatory vocal folds pro-liferated more significantly ,but the osteogenic differentiation and adipogenic differentiation of VF -MSCs in inflammatory vocal folds were restrained .Conclusion Inflammation enhanced the compressive resistance ,abated the elasticity , and restrained the multi -directional differentiation of VF -MSCs .