1.N-methyl-D-aspartate receptor NR2B subunit and neuronal apoptosis
International Journal of Cerebrovascular Diseases 2010;18(4):306-310
Neuronal apoptosis is one of the important forms of neuronal death in ischemic stroke.N-methyl-D-aspartate (NMDA) receptor-mediated excitatory amino acid toxicity plays an important role in neuronal apoptosis. It is the initiator and executor of apoptosis in neuronal cells. NR2B is the major regulatory subunit of this receptor, its transmembrane segment M2 is a reflexed membrane loop region facing cytoplast toward intramembrane, forming the inner wall of ion channel, which determines the permeability of the NMDA receptor ion channel to calcium. Glutamate mediates cell injury mainly through calcium overload, therefore, NR2B subunit plays a vital role in the neuronal apoptosis in ischemic stroke.
2.A randomized controlled clinical trial of acupuncture to prevent nausea and vomitingafter breast surgery
The Journal of Practical Medicine 2015;31(23):3950-3952
Objective To investigate whether acupuncture can prevent postoperative nauseaand vomiting (PONV)in patients received breast surgery. Methods Two hundred and forty patients were randomly divided into the acupuncture group and the control group. Patients in the acupuncture group were treated at bilateral Hegu (L4) and Neiguan (P6) after surgery. Patients in the control group received no any interventions. The occurrence of postoperative nausea and vomiting, the number of complete remission and the remedial treatment were record ed. Results The incidences of nausea and vomiting in the acupuncture group were lower than those in the control group from 2 h to 6 h post-operation, with less need of remedy treatment (P < 0.05, respectively). Conclusion Acupuncture in preventing nausea and vomiting after breast surgery owns a certain value in the early postoperative (6 h) stage, with effectively reducing the occurrence of postoperative nausea and vomiting.
3.Progress on phenotypic and genotypic characteristics of hypervirulent Klebsiella pneumoniae
Chinese Journal of Clinical Infectious Diseases 2014;7(2):179-182
Klebsiella Pneumoniae is an important pathogen for various infections clinically,in which hypervirulent Klebsiella pneumoniae (hvKP) mainly causes pyogenic liver abscess.By now,there are no standardized method to identify hvKP strains.hvKP strains are usually with hypermucoviscous phenotype,and the prevailing serotypes and clonal types are K1,K2 and CC23,CC65,respectively.Genes rmpA,magA,alls and kfu contribute to the pathogenicity of hvKP.This paper reviews the identification,clonal types and serotypes,hypermucoviscous phenotype and some other virulent genes of hvKP.
4.How to strengthen communication between department of microbiology and the clinical departments
Chinese Journal of Laboratory Medicine 2013;(4):375-376
With the rapid development of clinical laboratory technology and clinical improvement,the discordance between department of microbiology and clinical departments got more and more.Based on the analysis of interface issues existed between them,we discussed and explored how to do a wonderful job to communicate with each other.(Chin J Lab Med,2013,36:375-376)
5.Attention should be paid to hereditary ovarian cancer
Journal of Xi'an Jiaotong University(Medical Sciences) 2017;38(5):625-632
The occurrence of 20 to 25 percent of ovarian cancer is associated with genetic factors.Hereditary ovarian cancer includes genetic ovarian cancer and familial ovarian cancer.The genetic characteristics of tumors and the development of gene testing technology make it possible to detect tumor-related mutational genes by testing the gene of the proband and thus identify the other family members at risk and intervene early so as to realize early prevention and detection of the cancer.The paper reviews the genetic basis and pathogenesis of hereditary ovarian cancer as well as methods of detecting mutational genes, management of the proband, problems at present, and disease prevention for high-risk individuals.It stresses that we should pay attention to hereditary ovarian cancer in clinical work, identify the proband, and make comprehensive evaluation of risks of the patients` relatives so as to provide individualized guidance and carry out the concept of precision medicine.
6.Detection and clinical significance of C reactivn protein and P-selectin in patients with ulcerative colitis
Lin YANG ; Yanping JIA ; Hong YANG
Chinese Journal of Primary Medicine and Pharmacy 2009;16(3):391-392
Objective To study the detection and clinical signitlcanee of C reactivn protein(ClIP)and P-seleetin(P-sel)in patients with ulcerative colitis(UC).Methods The levels of peripheral blood ClIP and P-sel in patients with UC(n=53)and normal control group(n=35)were detected and the effects on disease severity were analyzed subsequently.Results The levels of blood CRP and P-sel in active phase group were significantly higher than those in catabasis group and normal controls group(P<0.05);severe stage and medium stage were signiticandy higher than those in mild stage(P<0.05,P<0.01),severe stage was significantly higher than those in medium stage(P<0.05);the blood level of CRP was positively related to P-sel in active phase group(r=0.638,P<0.01).Conclusion CRP and P-sel were correlated with invasion and severity of UC.
7.Effection of immune memory of tuberculosis vaccine on memory T cell in mice
Yang YANG ; Qianqian XU ; Yanping LUO
Journal of Chinese Physician 2012;(z2):4-6
Objective Exploring different strengthening immune strategy on long-term immune memory effects for tuberculosis vaccine lays the theoretical foundation.Methods The fusion protein AMM,adjuvant DDA and BCG-PSN mixed built AMM subunit vaccine.Choose only 50 C57BL/6 mice (SPF),according to the table of random number method was divided into 5 groups and each group 10 only,the experiment 1 group injected mice to phosphate buffer solution (PBS) as control; Experiment 2 mice only injected BCG vaccine (BCG) from early; Experiment 3 mice BCG initial free,in 10 weeks with AMM subunit vaccine strengthen immunity; The four mice from early after BCG,respectively on 8 weeks,10 weeks with AMM subunit vaccine strengthen immune mice one time.Experimental 5 groups of mice after BCG initial free,respectively on 6 weeks,8 weeks,10 weeks with AMM subunit vaccine strengthen immune mice a.28 weeks to remove spleen lymphocytes in mice,add 2.5μg/ml Ag85B stimulus 72 h after collecting spleen cell culture supernatant,with ELISA test Ag85B specificity splenic lymphocyte caused IFN ppar-gamma level.Results Spleen cells by antigen Ag85B stimulation,BCG initial free,AMM subunit vaccine strengthen immune once,twice,three times group and PBS compared all had difference (P <0.01 orP <0.05) ;BCG initial free-AMM subunit vaccine strengthen immune once,twice,three times group and BCG compared all had difference (P <0.01 or P <0.05) ; BCG initial free-AMM subunit vaccine strengthen immune once and twice or three times group compared with difference (P <0.01 orP <0.05) ; Initial free-AMM subunit vaccine strengthen immune two group and three times than group difference was statistically significant (P >0.05).Conclusion Mice strong immune strengthened the number is not the more the better,the appropriate number of strengthen the immune to induce the most lasting immunity memory,subunit vaccine strengthen immunization strategy may affect the immune memory is one of the important factors.
8.Research on medication rules of urticaria treatment based on data mining
Yanping YANG ; Jiarui WU ; Huirong MA
International Journal of Traditional Chinese Medicine 2015;(4):349-352
Objective A traditional Chinese medicine (TCM) inheritance assist system software was used to analyze the medication experiences of treating urticaria.Methods Literatures in three TCM medical books about the treatment of urticaria were collected and analyzed in terms of the utilizing frequency and association rules between different medicines. Data mining research methods such as Apriori algorithm, complex system entropy cluster and unsupervised hierarchical cluster were used in the research.Results Based on 138 screened prescriptions, the study confirmed that the frequency of using Radix sileriswas was highest, and 20 drugs elected in 218 prescription drugs were used more than 20 times. According to the utilizing frequency order of pharmaceutical compositions from high to low, association rules between drugs ranked in the top four were‘ Radix sileris- Cicada slough’, ‘Radix sileris- Schizonepeta’, ‘Radix sileris- Paeonia’, and ‘Schizonepeta- Cicadaslough’. Meanwhile, 30 pharmaceutical drug combinations and 14 new prescriptions were found. Conclusion The research, based on TCM inheritance assist system software platform and data mining method on medication rules of urticaria treatment prescriptions, is instructive in the clinical treatment of urticaria and also provide useful reference for the development of the new drug on urticaria.
9.The protective effect of tumor necrosis factor-?antibody on lung injury after cardiopulmonary bypass
Yang YU ; Shulin JIANG ; Yanping LIU
Chinese Journal of Thoracic and Cardiovascular Surgery 2003;0(03):-
Objective: To study the protective effect of anti-tumor necrosis factor-?antibody (TNF-? Ab) on lung injury after cardiopulmonary bypass(CPB). Methods: 20 patients with valve diseases underwent valve replacement under CPB. The patients were randomly divided into two groups: control group and TNF-? Ab group. In the TNF-? Ab group, human TNF-? Ab was dropped into the intracheal tube before operation and just after releasing the aortic clamp, respectively. Lung dynamic compliance, blood neutrophils count and TNF-? from the right and left atrium were determined perioperatively. Results: The dynamic compliance of the lung in TNF-? Ab group was higher than that in control group. TNF-? Ab can reduce releasing of TNF-? and restrain leukocyte accumulation in the lung. Conclusion: TNF-? Ab has markedly protective effect on lung injury after CPB.
10.Effects of Neurotrophics and Rehabilitation on Neural Function after Hypoxic-ischemic Brain Injury in Rats
Mei HOU ; Huijuan YANG ; Yanping JIANG
Chinese Journal of Rehabilitation Theory and Practice 2012;18(3):226-229
Objective To investigate the effects of neurotrophics and rehabilitation on the neural function recovery after the hypoxic-ischemic brain injury in rats. Methods Model rats of hypoxic-ischemic brain injury were given monosialotetrahexosylganglioside sodium(GM1, n=8), mouse nerve growth factor (NGF, n=8), both drugs (n=8), rehabilitation (n=8), both drugs+rehabilitation (n=8) and control (nointervention, n=8). The other 7 rats was as sham group. They were assessed with hanging and inclined plane test when 30 days old. ResultsIn the factorial design, main effect of GM1 was significant (P<0.05) but NGF (P>0.05) in hanging test; while both were significant in inclinedplane test. The interaction was not significant (P>0.05) in both tests. The main effects of drugs and rehabilitation were significant (P<0.05), as well as the interaction (P<0.05) in both tests. Conclusion GM1 may significantly improve muscle strength, voluntary movement,balance and coordination functions, and NGF may improve the balance and coordination functions. No interaction is found when combinedwith the two therapies. Rehabilitation may significantly improve muscle strength, voluntary movement, coordination and balance, and synergyeffects can be gained when combined with the drugs.