Autism in children is a complex disease of developmental disorder in nervous system,characterized by social deficits,impaired language and communication,stereotyped and repetitive behaviors.Studies indicate oxidative stress is associated with autism.Because of genic and environmental factors,children with autism are susceptible to oxidative stress.Oxidative stress induce the development of autism through abnormal metabolism of the membrane lipid,inflammation and excitotoxicity,autoimmunity,energy metabolism disorder,et al.Anti-oxidation might be a safe and effective therapy for autism.

Objective To compare and analyze two methods for determination of vaccine particle content in freeze-dried human rabies vaccine(Vero cells)bulk and final bulk,and to provide experimental basis for establishing standard detection method for particle content and purity of rabies vaccine products.Methods The samples were subjected to vaccine particle cracking,BCA quantification,and size exclusion chromatograghy-high performance liquid chromatography(SEC-HPLC)analysis. BCA-SEC relative area method and BCA-SEC vaccine particle standard curve method were used to detect the total protein concentration of the bulk and final bulk,separately,and the content of vaccine particles was quantitatively analy-zed. Finally,the detection results of the two methods were compared and analyzed.Results The content of vaccine particles in freeze-dried human rabies vaccine bulk was determined to be within 256-305 μg/mL by the two methods,with an average value of 267-285 μg/mL,and the relative standard deviations(RSDs)ranged from 5. 8% to 10. 2%,with good consistency between two methods. The content of vaccine particles in freeze-dried human rabies vaccine final bulk was determined to be in the range of 149-169 μg/mL by the two methods,with an average value of 152-164 μg/mL,and the two methods showed the RSDs between 0. 9%-4. 7% with good consistency.Conclusion The measured value by BCA-SEC standard curve method deviates less from the expected value and is closer to the actual situation of samples,so it is recommended to use this method as a reference for enterprises.

The "opening-closing-pivoting" theory on the six meridians is one of the difficult points in the study of Shang Han Lun (Treatise on Cold Diseases). The authors give an explanation on the space and time differentiation of three-yin and three-yang as well as the "opening-closing-pivoting" theory based on the predecessor's experience, and then analyze the physiological and pathological characteristics of the six-meridian disorders.

Objective To investigate the current situation of healthcare-associated infection (HAI)management in basic level private medical institutions.Methods 118 basic level private medical institutions in a district of Chengdu were investigated through visiting and questionnaire.Results Of 118 medical institutions,only 2 had HAI manage-ment personnel,4 had HAI management system.22.03% had hand washing facilities in therapeutic rooms and con-sulting rooms,37.29% of institutions stored aseptic items in accordance with the requirements,55.93% of institu-tions disposed medical waste at disposal center;99.15% handled reusable items by users.Conclusion HAI manage-ment status is worried,health administrative department should strengthen support and supervision,especially in-tensified the supervision of private dental clinics.

Paroxysmal nocturnal hemoglobinuria (PNH) is a rare disorder of hematopoietic stem cells due to acquisition of somatic mutations.Somatic mutations in phosphatidylinositol glycan class A (PIGA) account for intravascular hemolysis and other PNH manifestations,but the pathophysiology of clonal expansion of PNH cells cannot be elucidated clearly.PNH is closely related to aplastic anemia and myelodysplastic syndromes.Today,the gold standard for PNH is flow cytometry to detect the absence or severe deficiency of glycosylphosphatidylinositol (GPI)-anchored proteins on white and red blood cells.However,PNH diagnosed by phenotype is a group of heterogeneous disease in pathogenesis.Eculizumab,a first-in-class monoclonal antibody that inhibits terminal complement,is highly effective in stopping intravascular hemolysis and improving quality of life.Further research on the pathogenesis of PNH would be helpful to understand the underlying reasons for PNH phenotype cells in different patients,improve differential diagnosis and more targeted and specific therapy.Research progress in recent years will be reviewed in this article.

Objective: Our aim was to evaluate the biocompability of porcelain compounded with nanostructured material. Methods：The proliferation of the NIH3T3 cells were investigated using a tetrazolium-based colorimetric assay(MTT assay).Maceration extract of four tested materials were added into culture plate .The samples were tested with ultraviolet spectrophotometer in 490 wavelength at 1，3，5，7 days after the addition of extract. Morphology of these cells was also observed with phase-contrast microscope. Results： There was no obvious difference among three kinds of prosthetic materials in the optical absorbance and the cells growing in the extract showed normal morphology. Conclusion: The toxicity graduation of the porcelain compounded with nanostructured material is 0. This material has a good biocompability and can be used clinically.

Objective To explore diagnostic value of the immune intensity of platelet ?-amyloid precursor protein(APP) and isoforms proportion for Alzheimer's disease(AD).Methods The APP immune intensity of platelets was evaluated by flow cytometric detection and the isoforms proportion of APP130/APP106 was also detected by Western Blot analysis in 31 AD cases, 22 vascular dementia (VD) patients , 28 neural degeneration patients and 30 healthy elders.Results There was no significant difference of APP immunity in the each group. The ratio of APP130/APP106 was significantly lower in the AD group compared with the groups VD, degenerative disorder and healthy elders(all P

The scientific indexes and methods for checking utilization and intact rates of analytical instruments are established by cybernetic principles, fozzy mathemetical mdthods, theories of systematic engineering, and actual conditions of working units. it is proved that the realization of scientific management and control to manipulators, instruments and working enviroment can raise the utilization and intact rates of analytical instruments, guarantee the assay quality of drugs, increase the efficiency of supervision and promote the research work in drug analysis. Definite social and economic results are thus obtained.

Dystonia is common in children with cerebral palsy. It can cause motor dysfunction, and is frequently associated with altered speech articulation, abnormal swallowing, and excessive drooling. Although trihexyphenidyl (Artane) is an anticholinergic agent with a long history of use in the management of dystonia in adults, information is limited regarding the use of trihexyphenidyl in children. This study reviewed the clinical experience of the use of trihexyphenidyl in children with cerebral palsy for dystonia, with special emphasis on benefits and tolerability.

Objective:The aim of quality control in the key process of clinical laboratory is to provide accurate and reliable and timely clinical laboratory test reports. Methods:Strictly enforce the national development ofmedical institution clinical laboratory management approach, and to quality control from the several important aspects, such as the basic information before clinical examination, clinical laboratory equipment, reagents management, software conditions, issue of test reports, which should ensure quality control throughout the entire process of testing. Results:Thought strengthening of clinical laboratory quality control can greatly improve the accuracy of test results, reduce the test results influence because of subjective and objective factors in the testing process, which will promote and improve the quality of clinical testing. Conclusions:It is important to the quality of clinical laboratory tests and hospital medical quality and safety. Thought strengthening quality control in the key process of clinical laboratory, accurate test data can be provide for clinical diagnosis and treatment, and then effectively improve the level of the hospital.