Atrial tumor myocytes (AT-1 cells) were derived from tumor-bearing mice in which SV40 T large antigen drives atrial natriuretic promoter. AT-1 cells possess many features typically seen in cardiac cells from other species, such as spontaneous beating, multiple ion channels and responses to drugs, etc. Major ion channels are present in AT-1 cells: sodium, calcium and potassium channels. Sodium channel in AT-1 cells is highly sensitive to blocker tetrodotoxin. L- and T-type calcium channels respond to classical blockers. An important outward potassium current in AT-1 cells is the rapidly-activating delayed rectifier (IKr). IKr Is the sole time-dependently repolarizing potassium current in AT-1 cells. AT-1 cells are a very useful tool for studies in cardiac electrophysiology and pharmacology. As yet, a great number of cardiac and non-cardiac drugs have been tested for their IKr blocking actions in AT-1 cells. The polymorphic ventricular arrhythmia (torsade de pointes) is a severe side effect of IKr blockers.

Objective To evaluate the efficacy of recombinant tissue-type plasminogen activator(rt-PA)and mild hypothermia for acute cerebral infarctions. Methods72 patients consistent with the inclusion criteria were randomly divided into 2 groups,hypothermia group receivied rt-PA combined with mild hypothermia and control group receiving rt-PA only.Neurological functions was evaluated with European Stroke Scale(ESS). ResultsAs intravenous thrombolytic therapy within 6h after the onset,the ESS scores examined 3 days after thrombolysis in hypothermia group was significantly higher than those in control group.As intravenous thrombolytic therapy during 6h to 12h after the onset,the ESS scores of hypothermia group increased more significantly from 24h after thrombolysis than those in control group. Conclusionft-PA combined with mild hypothermia was more effective than rT-PA only for acute cerebral infarctions,which was effective and relatively safe in the treatment of acute ischemic cerebral vascular disease.

Objective To compare of pregnancy induced hypertension caused postpartum hemorrhage by two treatment methods. Methods 104 patients with pregnancy induced hypertension were randomly divided into misopr-ostol plus oxytecin group ( observation group)54 cases and the control group ( 50 eases ), used oxytcoin alone, respec-tively, Results postpartum hemorrhage in The observation group of is five cases(9.2% )which was significantly low-er than the control group of 11 cases(22.0% ) ( X2= 3.95 ,P<0.05 ). average postparttun hemorrhage in observation group is ( 195.6±88.4) ml which was significantly lower than the control group (303.2±111.6) ml (t=2.31, P<0.05). Conclusion Misoprostol combined with oxytocin is effective, safe and convenient to patients with postpartum hemorrhage caused by pregnancy induced hypertension.

Objective:To study the influence of cusp inclination of artificial posterior teeth on abutment displacement of unilateral distal-extension telescope crown denture.Methods:Study models of mandibular unilateral distal-extension and telescope crown dentures with the cusp inclination of 0?,12?,25? and 33? were respectively prepared. When food chip was masticated on the occlusal surface and the dentures were loaded by 29.4 N, 49 N or 68.6 N, the abutment displacement was measured.Then the data were calculated and reciprocally compared with statistical analyses. Results:Between anatomic and 25? semi-anatomic artificial posterior teeth, there were significant differences in the buccolingual and vertical abutment movement(P0.05). The parallel, inclination, twist-like and vertical movement of abutment increased with the increase of the load on the denture. The difference among different level of load was statistically significant (P

According to the national residency training regulations in the teaching of clinical and scientific research, the department of thoracic surgery of First Affiliated Hospital of Chongqing Medical University has used seminar teaching , and has collected random sampling survey questionnaire of 110 students participating in seminar from September 2014 to March 2016. The investigation shows that through seminar teaching training, doctors will cultivate their clinical and research ability, and enhance their self-confidence. Therefore, the application of seminar teaching in department of thoracic surgery is helpful to improving the quality of clinical teaching and is worthy of further improvement and promotion.

Objective To discuss the correlation between the dysmenorrhea and TCM constitutions among the junior high school girls of Shanghai suburb; To analyze the influencing factors; To provide references for the prevention of dysmenorrhea. Methods By using cluster sampling during December 2015 to January 2016, the questionnaire surveys which included the general situation, the classification and determination of TCM constitution self-test and COX Menstrual Symptom Scale were used for the junior high school girls from the suburb area of Shanghai to analyze the relationship between dysmenorrhea and TCM constitutions and the influencing factors. Results The incidence rate of dysmenorrhea was 29.52% (93/315) in junior high school girls from the suburb area of Shanghai. There was statistical significance in body height and menarche age among girls with and without dysmenorrhea (P<0.05). The rank correlation coefficient of dysmenorrhea and gentleness type was -0.130 (P=0.021). The rank correlation coefficients of dysmenorrhea and pathological constitution (except for special diathesis type) were all greater than 0 (P<0.05). Univariate analysis showed that there was statistical significance in the incidence of different coagula in menses, different inheritance of dysmenorrhea, different sleep quality, different learning pressure, different emotional state and different physical exercises (P<0.05). Logistic regression analysis showed that influence factors of dysmenorrhea were inheritance of dysmenorrhea, learning pressure and yin-deficiency constitution. Conclusion The pathological constitution is the susceptible constitution of dysmenorrhea of junior high school girls. Yin-deficiency constitution, inheritance of dysmenorrhea and learning pressure can increase the incidence of dysmenorrhea. It is suggested that the corresponding preventive measures should be taken to achieve the purpose of prevention dysmenorrhea by regulating and improving yin-deficiency constitution.

OBJECTIVE To investigate the drug-resistance status of Escherichia coli(ECO)from various kinds of specimens to provide the scientific evidence for reasonable use of antibiotics.METHODS ECO strains were isolated and identified according to the National Clinical Laboratory Operation Rules.Drug resistance profile was analyzed by K-B method recommended by CLSI.RESULTS Among 275 ECO strains,50.2%(the most highest isolating rate)were isolated from urine.Detection rate of extended spectrum ?-lactamases(ESBLs)producing ECO was 32.4%(89/275).Except for 100% susceptibility to carbopenems,the drug-resistance to 14 antibiotics in ESBLs producers was higher than in the nonproducers.CONCLUSIONS Laboratory department should think highly of the monitoring of ECO to prevent and control the occurrence and prevalence of the nosocomial infection with ECO.

The clinical puncture skills are the elementary technical skills for every medical student.In recent years,reforms have been carried out on teaching method of the training of clinical puncture skills in our hospital and we have achieved satisfactory teaching effect.These reform measures include reforming of operating flow,repeated training from simulative training to clinical practice,educating the medics'consiousness of medical safety and cultivating their competence of communication with patients.

Objective To evaluate the hospitalization charges of medical-insured patients with different in-comes, different age and the burdens shared by individuals, to analyze the multiple factors that influence the charges and provide basis for goverment policy making. Method We investigate the medical costs and incomes of 1588 medi-cal-insured inpafients in a Nantong hospital ,study the expenditure burdens and influencing factors of patients with dif-ferent income and different age appling T-test,F-test,and χ2 test. Results There is singificant difference in expendi-ture burdens between patients of different income level ,the expenditure burdens are obviously heavier in group of old age patients than in other age groups. Conclusion It is necessary to reinforce the government functions and perfect the health security system, low income groups should be given more attentions and cares.

The emphasis and basic target of medical education is to cultivate medical students with high qualities. The qualities of medical students include professional ethics, self-cultivation, medical knowledge and physical-psychological quality, which is critical to the medical education. It has been proven that the teaching of Clinical Pathway is one of the most important factors to promote the improvement of comprehensive qualities of medical students.