Objective:To investgate the definite spot of expression of leukemia inhibitory factor gene in mouse endometrium in the implantation window phase.Methods:By reverse transcription-polymerase chain reaction(RT-PCR),the expression of LIF gene of 20 cases of the implantation sites and the paraimplantation sites from the endometrium of the mice that were 4 days pregnant was detected.Results:The average level of LIF mRNA expression of the implantation sites was 0.5316.The average level of LIF mRNA expression of the paraimplantation sites was 0.3711.By the statistic t-test,the difference was significant( P

Objective To study the efficacy of paroxetine in the treatment of chronic obstructive pulmonary disease( COPD) with depression.Methods 110 COPD patients with depression were randomly divided into the observation group and control group according to the random number table method,55 cases in each group,the control group was given psychological counseling while the observation group received paroxetine on basis of the control group.The clinical effect,arterial oxygen pressure( PaO2 ) ,arterial partial pressure of carbon dioxide( PaCO2 ) ,forced expiratory volume in one second(FEV1),Hamilton Depression Scale(HAMD) and adverse reaction were compared between the two groups.Results The total effective rate of the observation group was 92.7%,which was significantly higher than 67.3%of the control group(χ2 =13.152,P=0.013);PaO2 ,PaCO2 and FEV1 of the observation group after treatment were (84.8 ±0.3) mmHg,(38.3 ±0.7) mmHg,(1.7 ±0.2) L,which were significantly improved than (67.8 ±1.2)mmHg,(65.3 ±0.8)mmHg,(1.5 ±0.1)L before treatment(t=9.798,10.132,9.693,P=0.029,0.018,0.026),which had a statistically significant difference with the control group after treatment(t =10.021,11.021,10.751,P=0.026,0.018,0.023);HAMD score of the observation group was (11.2 ±0.4)points, which was significantly lower than (17.9 ±0.7)points before treatment(t=9.915,P=0.032),which had a statisti-cally significant difference with the control group after treatment(t=11.723,P=0.031);The adverse reaction of the two groups had no statistically significant difference(χ2 =5.152,P=0.073).Conclusion The application of parox-etine has a good curative effect in the treatment of COPD complicated with depression,which can significantly improve the clinical symptoms of the patients,with no serious adverse reactions.

Objective To explore the relationship between primipara′s mother role identity with depression and anxiety . Methods Totally 213 primiparas in our hospital were selected .The self -designed Primipara′s Mother Role Recognizing Scale , Self‐Rating Anxiety Scale (SAS) and Self‐Rating Depression Scale(SDS)were applied to measure the mother role identity ,anxiety and depression degrees before delivery ,at postpartum 1 week and puerperium period .Results The anxiety value at postpartum 1 week were extremely significantly higher than that before delivery and at the puerperium period (P<0 .01);the depression value at postpartum 1 week were extremely significantly higher than that before delivery and at the puerperium period (P<0 .01);the total scores of role playing ,role adaptation and role identity at the puerperium period were significantly higher than those at postpartum 1 week(P<0 .05);primipara′s anxiety and depression were negatively correlated with the primipara′s mother role identity (r=-0 .03- -0 .30) .Conclusion The degree of mother role identity is negatively correlated with the parturition event progress ,iden‐tity process and moods of depression and anxiety .

Periostin, a extracellular matrix protein structurally similar to fasciclin proteins, is expressed specifically in various tumor tissues and correlates with tumor progression and prognosis. Periostin prevents the apoptosis of tumor cells, promotes tumor growth and can induce angiogenic signaling cascades through αvβ3-FAK and Flk-1/KDR. It also contributes to epithelial-mesenchymal-transformation promoting tumor invasion and metastasis. Periostin maybe a potential therapeutic target of cancer treatment.

Bilirubin ; analysis ; Child ; Electric Impedance ; Esophagus ; metabolism ; Gastroesophageal Reflux ; diagnosis ; therapy ; Humans ; Hydrogen-Ion Concentration ; Surveys and Questionnaires

OBJECTIVE:To observe the efficacy and safety of alprostadil combined with tanshinone ⅡA in the treatment of pa-tients with early chronic kidney disease(ECKD). METHODS:138 ECKD patients were randomly divided into group A,group B and group C,46 cases in each group. On the basis of conventional treatment,group A received 40 mg TanshinoneⅡA sodium sulfonate in-jection,adding into 250 ml 0.9% Sodium chloride solution,ivgtt,qd+10 μg Alprostadil injection,adding into 100 ml 0.9% Sodium chloride solution,ivgtt,qd;group B received the same dose with Alprostadil injection;group C received the same dose with Tanshi-noneⅡA sodium sulfonate injection. All patients were treated for 14 d. Clinical efficacy,laboratory indexes [24 h urine protein,serum creatinine(SCr),total cholesterol(TC),triglyceride(TG),blood urea nitrogen(BUN),uric acid(UA)] in 3 groups before and af-ter treatment were observed,and the incidence of adverse reactions was compared. RESULTS:The total effective rate in group A was 97.83%,which was significantly higher than group B(80.43%)and group C(76.09%),the differences were statistically significant (P<0.05). Before treatment,there were no significant differences in 24 h urine protein,SCr,TC,TG,BUN and UA among 3 groups (P>0.05);after treatment,all above-mentioned indexes significantly reduced,and group A was lower than group B and group C,the differences were statistically significant(P<0.05). There was no significant difference in the incidence of adverse reac-tions among group A(10.87%),group B(6.52%)and group C(8.70%)(P>0.05). CONCLUSIONS:Alprostadil combined with tanshinoneⅡA shows good efficacy in the treatment of ECKD,it can improve renal function in patients with good safety.

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Objective To explore the surgical treatment of intra -articular calcaneal fractures with bone nodules joint angle in the preoperative and intraoperative evaluation of prognosis of postoperative analysis.Methods 22 cases of affected joint surface after calcaneal fractures,open reduction and internal fixation with steel plate in the operation treatment.Used Sanders classification:preoperative,intraoperative,postoperative again calcaneal tubercle joint angle measurement.According to the Maryland foot scoring system to evaluate postoperative effect.Results All patients were followed up for 8 to 20 months.The excellent and good rate of the patients was 90%,the postoperative complications:heel broadening,subtalar arthritis in 2 cases,poor healing in 2 cases (after the treatment and healing). Conclusion It has high value of the calcaneal tubercle joint angle measurement in the joint surface after calcaneal fractures.

Objective To compare the clinical therapeutic effects of thyroid endoscopic surgery and traditional open surgery in treatment of thyroid diseases.Methods Data of 136 patients undergoing thyroid surgery from May.2011 to May.2012 in the Second People's Hospital of Yunnan Province were collected.They were randomly divided into 2 groups:the observation group (n =68) and the control group (n =68).The observation group were treated with endoscopic thyroid surgery and the control group with the traditional surgery.Clinical data of the 2 groups such as operation time,intraoperative blood loss,postoperative drainage volume,postoperative serum interleukin 2 (IL-2),C reaction protein(CRP),TNF-αexpression level,postoperative complications,postoperative hospital stay and so on were collected and compared.Results The difference had no statistical significance between the 2 groups in the operation time,intraoperative blood loss,postoperative drainage volume,postoperative serum IL-2,CRP,TNF-αexpression levels,and postoperative complications (P ＞ 0.05).However,the observation group had superiority in the postoperative hospital stay,neck discomfort and the acceptability than the control group.The difference had statistical significance (P ＜ 0.05).Conclusions Endoscopic thyroid surgery is safe and reliable.Compared with traditional open surgery,it has better cosmetic effects and less trauma.

Objective To investigate the status and influencing factors of nurses' cognition to premature parents' health education demands,so as to provide reference for nursing leaders to take intervention.Methods 59 NICU nurses from two hospitals in Guangzhou and Shenzhen were chosen by convenience sampling.A self-designed questionnaire was used to investigate.Results A total of 56 questionnaires was recovered,with a rate of 94.92％.NICU nurses perceived the degree of parents total education demands was (3.20±0.48) points.The demands of knowledge about diseases progress and related diagnosis and nursing were high,reaching (3.33±0.47) points.Breast feeding got the highest score,(3.53±0.69)points.Ed-ucation,professional ranks,post of nurses had no correlation with total score,but ages and length of service were negatively correlated with general demand,mental nursing and social support.Conclusions NICU nurses value premature parents' health education,and nursing leaders should take interventions to prevent occurrence of job burnout.