Objective:To investigate the effects of IL-2 on the proliferation activity and HCG secretion of first trimester human trophoblastic cells in vitro. Methods:Growing -well 4th generation trophoblastic cells were selected,cultured for 48 h with IL-2 of different concentration. The MTT colorimetric assay was used to analyze the proliferation activity, and radioimmunoassay was used to analyze the secretion of HCG. Results:①Low concentration (100 U/ml,400 U/ml) IL-2 promoted proliferation activity and HCG secretion (P

Ecological charactcristics and biological specific feature of Helleborus thibetanus Fran ch. were briefly described and cultivation technique to transform the wild plant into itscultivated variety was studied to provide a basis for the protection and expliotation ofthe wild resource of H. thibetanus.

Objective:To observe the effect of nape acupuncture on vertebral-artery type cervical spondylosis.Methods:Eighty patients with vertebral-artery type cervical spondylosis were randomly divided into nape acupuncture group and Jiaji acupuncture group,with 40 cases in each group.The patients in the nape acupuncture group were treated with the nape acupuncture plus Jiaji acupuncture,while the patients in the Jiaji group were treated with Jiaji acupuncture only,to observe the functional scale before and after treatment.Results:The total effective rate in the nape acupuncture group was 95.0%,while the total effective rate in the Jiaji acupuncture group was 82.5%.By Ridit analysis,u=5.186,P＜0.01.It indicated that the therapeutic effect in the nape acupuncture group was better than that in the Jiaji acupuncture group.Compared with Jiaji acupuncture group,the difference value of pre- and post-treatment about functional scale of vertebral-artery type cervical spondylosis and the post-treatment score have statistic differences (P＜0.01).It indicated that the therapeutic effect of nape acupuncture group was more obvious in treating vertigo than that in the Jiaji acupuncture group.Conclusion:The treatment of vertebral-artery type cervical spondylosis by Jiaji acupuncture plus the nape acupuncture may enhance the therapeutic effect significantly.

Objective To assess whether urapidil (anα1 receptor blocker)is more effective than ni-troglycerin in treatment of non-valvular heart failure accompanying hypertension and diabetes mellitus (DM ) in elderly female patients .Methods Fourty-six elderly female patients with non-valvular heart failure accompanying hypertension and DM were randomly divided into urapidil treatment group (n= 24) and nitroglycerin treatment group (n= 22) .Patients in urapidil treat-ment group were treated with urapidil (50 -300 μg/min) and those in nitroglycerin treatment group were treated with nitroglycerin (5 -20 μg/min) for 48 -140 h according to their blood pressure (BP) and cardiac function .Their BP ,HR ,and metabolic activity were observed before and on days 1 ,2 ,3 and 7 after treatment .Results The effect of urapidil was significantly lower than that of nitroglycerin on SBP on day 7 after treatment (P<0 .05) .The serum NT-proBNP level was significantly lower in nitroglycerin treatment group than in control group (P<0 .01) . Conclusion The effect of urapidil is better than that of nitroglycerin on reducing and stabilizing SBP ,attenuating cardiac afterload ,and improving cardiac function in elderly female patients .Ura-pidil can thus be used as one of the first choiced drugs in treatment of elderly female patients with non-valvular heart failure accompanying hypertension and DM .

After the necessity of research on bio-entities was pointed out, the advances and status quo in research on the relation between bio-entities were analyzed with concrete ideas and project put forward for the research on bio-entities using scientometric methods according to thecitationrelation in bio-entities similar to that in litera-ture.This kind of research will promote and perfect the research on scientometric theory and practice, and open a new visual angle for the research on knowledge management .

The metabolic syndrome,as a group of concurrent cardiovascular risk factors,is attributed to both genetic and environmental causes and correlated with many gene polymorphisms and their mutations,such as adiponectin gene,peroxisome proliferator activated receptor gamma gene,lamin protein A/C gene,uncoupling protein 2 gene,?-adrenic receptor gene and some other inflammation marker genes.This paper reviews the advances in the studies of the gene polymorphisms associated with the metabolic syndrome.

For a valid control of the finance management, the management system is developed again aiming at finance related control links and key control points. In this way, the control is rigorous and integral with simple procedures for check and examination. The key control point is set oriented to note management. Real-time operation is controlled. Post output processing is controlled. With business income and business expenditure as the main line, the control process of the related links are built up. Computer maintenance system is controlled.

Objective To explore the clinical efficacy of Toutongning capsule combined with nimodipine in the treatment of 76 patients with primary vascular headache.Methods 76 patients with primary vascular headache were selected as the research subjects.According to the different surgery,the patients were randomly divided into control group (n =38) and observation group (n =38).The control group was given compound Danshen injection,while the observation group was given Toutongning capsule combined with nimodipine.The efficacy,the peak systolic velocity(Vs) and pulsatility index(PI) of each vessel,the time of stopping pain,recurrence rate and adverse reaction were compared between the two groups.Results The total effective rate of the observation group was 94.74％,which was higher than 84.21％ of the control group,the difference was statistically significant (x2 =4.785,P ＜ 0.05).The MCA,ACA,BA,VA value of Vs and PI,the time of stopping pain and recurrence rate in the observation group were better than those in the control group (t =5.385,6.834,7.407,6.651,4.032,5.716,6.203,5.016,4.785,x2 =5.473,all P ＜ 0.05).There were no significant differences between the two groups in PCA value of Vs and PI and adverse reaction(t =0.283,0.037,x2 =0.314,all P ＜ 0.05).Conclusion Toutongning capsule combined with nimodipine in the treatment of primary vascular headache has high application value and significant effect,and it can promote cerebral circulation,blood flow,time of stopping pain and recurrence rate,so it is worthy of clinical popularization and application.

With the increasing trade of traditional Chinese medicine(TCM),the issue of excessive levels of toxic heavy metals metals in TCM has raised worldwide concerns. Some countries and re?gions have set strict limits on the heavy in TCM. There are currently no uniform limits on heavy metals, partly for lack of a good understanding of heavy metals in TCM. The toxicity of heavy metals varies with their chemical forms so that it is not rational to evaluate safety in terms of total contents. Cinnabar and realgar are widely used TCM containing heavy metals in clinical practice. Because of the potential toxicity of heavy metals such as mercury or arsenic,and the related adverse reactions reported occa?sionally,the safety of cinnabar,realgar and their preparations has also attracted public attention. In the present paper,the progress in toxicological studies on cinnabar,realgar as well as commonly used preparations was reviewed while priorities of future research were proposed.

ObjectiveTo study the diagnosis of thyroid cancer concurrent with multiple thyroid nodules.Methods The clinical data of 23 patients of multiple thyroid nodules with thyroid cancer undergoing exploration from May 1988 to December 2000 were analyzed retrospectively.ResultsThe diagnosis of concurrent carcinoma was made preoperatively in 6 cases. 13 out of the 23 patients underwent second operation for the postoperative pathology being malignant. Thyroid cancer was identified concurrent with benign nodules in 20 cases. Conclusion Thyroid cancer when found on the background of multiple thyroid nodules is often coexistant with benign thyroid disease, hence the diagnosis is difficult to make clinically. Second operation is sometimes inevitable when the pathology identified a cancer in preoperatively diagnosed benign nodules.