Objective To investigate the effect of Helicobacter pylori (Hp) infection on plasma lpid and high sensitive C reactive protein (hs-CRP) levels in patients with cerebral infarction, and explore the pathogenesy. Methods Seventy-nine cerebral infarction patients without nearly inflammatory reaction disease were recruited. Hp was detected by breath test,the patients were divided into infection-negative group (15 cases), light infection group (29 cases) and severe infection group(35 cases) according to the results,the last two groups were as infection-positive group. The plasma lipid and hs-CRP levels were exanined.Results Compared with infection-negative group,the total cholesterol,low density lipoprotein,hs-CRP levels increased obviously in infecton-positive group (P ＜ 0.05). The level of hs-CRP in severe infection group was higher than that in light infection group [(10.21 ±4.98) mg/L vs. (5.81 ±4.21) mg/L](P=0.001 ). Conclusions Hp infection may increase vascular inflammatory reaction through lipid metabolic disturbance. The cerebral infarction with higher Hp infection, and with the degree of infection increased, the risk is also increased accordingly.

By consulting the relevant literature and historical herbal literature,the name,origin and harvesting,efficacy and indications,as well as nature,taste,and meridians of Bunge pricklyash seed were reviewed."Jiaomu"as a medicinal herb name was first recorded in the"Compendium of Materia Medica Annotations",and later generations also used"Jiaomu"as a proper name.Bunge pricklyash seeds are the seeds of the Rutaceae plant Sichuan Bunge pricklyash seed or green Bunge pricklyash seed.They are harvested when they mature in autumn from August to October,and can be processed by net,stir-frying or salt.It can promote diuresis and reduce swelling,treat kidney deficiency and tinnitus,and is mainly used to treat bloating in the abdomen.It is non-toxic or slightly toxic.This article traced the origin of Bunge pricklyash seed,studied the history of medicine,clarified the original traditional Chinese medicine properties of Bunge pricklyash seed,and provided scientific basis for the correct clinical use and the national formulation of quality standards for this herb.

Epilepsy is a common neurological disease, which requires long-term seizure control with medication. As a particular population, breastfeeding safety and the use of antiepileptic drugs during breastfeeding are significant concerns for those at childbearing age, as it involves maternal health and the growth and development of the offspring. This article reviews the latest evidence regarding breastfeeding in women with epilepsy as a reference for clinicians.

Objective: To establish a hydrogen peroxide (H₂O₂) induced injury model of pulmonary artery endothelial cells (PAECs) and explore the molecular mechanisms of oxidative stress on the structure and function of PAECs in this model. Methods: Human PAECs were treated with H₂O₂ at different concentrations (25, 50, 100, 200, 400, 800, 1 600, 3 200, 6 400 μmol/L) for 4 and 24 h, respectively. The PAECs survival curve was obtained according to the cell viability measured by CCK-8 assay. The cell apoptosis of PAECs was detected by flow cytometry. The reactive oxygen species (ROS) generation and mitochondrial activity were measured using small molecule fluorescent probes. Proteins were extracted and the phosphorylation levels of signal molecules in PAECs were detected by Western blot assays. Results: (1) The effect of H₂O₂ at various concentrations on cell viability of PAECs: cell viability of PAECs decreased in proportion to increasing concentration of H₂O₂ after incubation for 4 h. The half maximal inhibitory concentration (IC₅₀) of PAECs exposed to H₂O₂ for 4 and 24 h were 397.00 and 488.77 μmol/L, respectively. (2) The effect of H₂O₂ on cell apoptosis of PAECs: After H₂O₂ incubation for 4 h, proportions of PAECs at late-apoptosis ((22.58±3.69) %) and necrotic stage( (11.86±4.27)%) were significantly higher than those of control PAECs at late-apoptosis stage( (3.41±1.44)%, P<0.01) and at necrotic stage ((1.94±1.15) % , P<0.05). The survival rate of PAECs post H₂O₂ was dramatically lower than that of control PAECs ((7.98±3.21)% vs. (48.89±8.08)%, P<0.01). However, there is no statistical difference between both groups regarding to the early apoptosis. (3) The effect of H₂O₂ on mitochondrial activity and ROS production of PAECs: the mitochondrial activity and ROS generation of PAECs treated by H₂O₂ were significantly increased compared to those in control PAECs (P<0.01). (4) The effect of H₂O₂ on signaling molecules in PAECs: there was a significant increase in phosphorylation level of Akt in PAECs incubated with H₂O₂ for 30 minutes compared to that in control PAECs (P<0.01), while there was no significant difference in levels of Akt between H₂O₂ treated PAECs and control PAECs. Phosphorylation level of JNK as well as p38 were also significantly upregulated in H₂O₂ treated PAECs (P<0.01). Conclusion H₂O₂ at the concentration of 400 μmol/L could induce human PAECs injuries via the regulation of Akt and MAPK signaling pathways.

[摘 要] 目的：探讨解偶联蛋白2（UCP2）在皮肤黑色素瘤（SKCM）中的预后价值，分析UCP2表达与SKCM肿瘤浸润免疫细胞（TIC）之间的相关性。方法：通过GEPIA在线数据库分析SKCM组织及正常皮肤组织中UCP的差异性表达，探讨UCP表达与SKCM预后之间的关系。HPA数据库分析UCP2在正常皮肤单细胞中的表达情况，使用TISCH分析UCP2在SKCM中单细胞水平的表达情况。TIMER分析UCP2表达与总SKCM、原发性SKCM和转移性SKCM患者肿瘤微环境（TME）中主要免疫细胞及其标志物的相关性。结果：UCP2在SKCM癌组织中高表达，UCP2高表达患者的OS和DFS均长于低表达患者（均P<0.05）。正常皮肤和SKCM单细胞分析显示，UCP2表达与免疫细胞亚群存在一定的正相关，同时与SKCM中趋化因子、受体、组织相容性复合物、免疫抑制剂和免疫激活剂五类免疫途径密切相关（均P<0.05）。亚组分析结果显示，UCP2高表达与总SKCM和转移性SKCM患者高OS密切相关（均P<0.05）。UCP2表达与总SKCM、原发性SKCM、转移性SKCM患者TME中TIC之间存在明显的正相关（均P<0.05），但是与原发性SKCM相关性相对较弱。UCP2表达水平与3组SKCM患者TME中免疫细胞标志物存在明显的正相关（均P<0.05）。结论：UCP2在SKCM组织中高表达，UCP2与SKCM中TIC呈正相关，是TME中重要的调控因子，与其预后密切相关。

[摘 要] 目的：探讨核因子κB抑制因子a（NFKBIA）表达与皮肤黑色素瘤（SKCM）患者预后及其与肿瘤微环境免疫浸润的相关性。方法：利用GEPIA2数据库分析正常皮肤和SKCM组织中NFKBIA的表达差异，GEPIA2和Ualcan数据库分析NFKBIA与SKCM预后关系，TIMER和TISIDB数据库分析NFKBIA与SKCM中TIL和免疫调节基因的关系。选用TISCH和CancerSEA数据库从单细胞水平分析NFKBIA与SKCM细胞亚群及其相关的功能状态关联性。选取湖北省荆门市第二人民医院保存的14例SKCM患者的石蜡组织标本，通过免疫组织化学染色法验证SKCM组织和癌旁组织中NFKBIA蛋白的表达水平。结果：NFKBIA在SKCM组织中呈低表达，并且低表达的SKCM患者预后差（P<0.05）。NFKBIA表达与B细胞、CD8+ T细胞、CD4+ T细胞、巨噬细胞、中性粒细胞和DC浸润水平呈正相关关系（均P<0.01）。NFKBIA表达与SKCM中TIL丰度和免疫调节基因呈正相关关系（均P<0.01）。NFKBIA在SKCM单细胞免疫细胞中表达，且与肿瘤微环境中细胞分化和炎症呈正相关关系（R=0.28、0.23，均P<0.05）。免疫组织化学染色结果证实，NFKBIA蛋白在SKCM组织中阳性表达率显著低于癌旁组织（35.71% vs 85.71%，P<0.05）。结论：NFKBIA在SKCM组织中呈低表达，与SKCM免疫细胞浸润相关，可作为SKCM预后的标志物及治疗靶点。