Based on the analysis of the current English level of the graduate students of our university, the author suggests some changes in the areas of listening, speaking， reading and writing.

Objective:To investigate the therapy and efficacy in patients with symptomatic congenital cyst of liver.Methods: Eighty-six cases of congenital cyst of liver admitted from Jan. 2000 to Dec. 2006 were reviewed retrospectively.Results: Injection of ethanol(17 cases):with 2 cases of recurrence(11%).open surgery(48cases):with 4 cases of recurrence(8.3%). And laparoscopic surgery(21cases):with 1 case of recurrence(4.8%),We are found as the therapies. Conclusions:Methods of therapy for congenital cyst of liver include:injection of ethanol into the cyst cavity,open surgery,laparoscopic surgery.Laparoscopic fenestration is of minor invasion,faster recovery and shorter hospital stay.

Objective To explore the effect of Kuhuang injection on liver function and serum trace elements levels in patients with acute jaundice hepatitis.Methods 37 cases of acute jaundice hepatitis patients from December 2013 to April 2015 were selected and randomly assigned into treatment group and control group, 19 cases in control group were treated with potassium magnesium aspartate injection, 18 cases in treatment group with Kuhuang injection on the basis of control group.The liver function and serum trace elements were compared pre-and post-treatment.Results Compared with control group post-treatment, the level of serum cholyglycine (SCG) was significantly lower(P<0.05), the levels of total bilirubin (TBIL) and direct bilirubin (DBil) were lower(P<0.05), magnesium, copper, zinc levels were higher(P<0.05) and level of iron were lower(P<0.05)in treatment group.Conclusion Kuhuang injection has good clinical curative effect for the patients with acute jaundice hepatitis, we speculated that it might be possible to improve the liver function and levels of serum trace elements .

Objective To explore a scientific and forward-looking medical materials classification and coding strategy by studying the medical materials classification and coding of extracorporeal circulation and blood purification.Methods From the clinical practice and based on the national standard and the industry standard,the classification coding framework was built through expert consultation and the research of literature and regulation.Results A set of medical devices coding system was proposed determining identifier position,type,name,material,specification and etc,which involved in 7 layers and 14 digits of codes.Each layer was represented by 2 bits of digital Arabia.The coding system fulfilled the requirements for medical facility internal management and centralized purchase bidding.Conclusion The classification and coding system for extracorporeal circulation and blood purification consumables eliminates the deficiencies in naming,promotes consumables information system and lays a foundation for consumables precision management and bidding.

Objective: This study proposed a possible molecular mechanism underlying centrosome amplification in oral squa-mous cell carcinoma (OSCC) by analyzing the relationship of centrosome amplification with the expression of STK15 and p53 in OS-CC tissues. Methods: The expression of STK15 and p53 in formalin-fixed, paraffin-embedded tissues from 8 patients with normal oral epithelium and 43 with OSCC were quantitatively analyzed by immunohistochemistry. Centrosome status was investigated by an indi-rect-immunofluorescence double-staining method to determine the message of centrosome amplification in OSCC. The correlation of the expression of the two proteins with centrosome amplification in OSCC was statistically analyzed with SPSS13.0. Results: Normal oral epithelium showed normal centrosomes in epithelium cells, whereas 33 of the 43 OSCC cases (76.74%) showed evidence of centro-some amplification. STK15 was undetectable in normal oral epithelium. The percentage of STK15 overexpression was 67.44% in OS-CC (P=0.028). The percentage of STK15 overexpression was significantly higher in OSCC with positive p53 staining than in OSCC with negative p53 staining (P=0.01). Spearman correlation analysis indicated a correlation between STK15/p53 positive co-expression and centrosome amplification of OSCC (P=0.019). Conclusion: Centrosome amplification is a common abnormal phenomenon in OS-CC. The p53/STK15 trans-activation-independent pathway plays a role in the systemic molecular regulation of centrosome in OSCC, which leads to the occurrence of OSCC.

AIM To investigate the effect of urapidil on NO, ET, ANF and AngⅡ of spontaneously hypertensive rats. METHODS The content of nitric oxide (NO), endothelin (ET), atrial natriuretic factor (ANF) and angiotensin Ⅱ (AngⅡ) were determined by spectrophotometry and radioimmunoassay of spontaneously hypertensive rat (SHR) during the process of decreasing blood pressure with urapidil. RESULTS\ They were as follows: compared with the same aged normal blood pressure WKY rats, in the SHR, the content of serum NO decreased, the level of ET and ANF in the plasma increased. Whereas the level of AngⅡ has no difference in any group. Compared with the control of SHR,urapidil can increase the contents of serum NO, decrease the level of ET and ANF in the plasma. CONCLUSION \ It is suggested that urapidil may adjust the imbalance of cardiovasco-active substances by increasing the release of endogenous vasodilators and reducing the release of endogenous vasocontrictors to antihypertention.

Objective To investigate the serum levels of anti-BRAF antibody in patients with rheumatoid arthritis (RA) and its clinical significance.Methods Blood samples from 129 patients with RA,71 patients with other rheumatic diseases and 68 healthy controls were measured by enzyme-linked immunosorbent assays (ELISA).The serum levels of anti-BRAF antibody in patients with RA were divided into high,medium and low groups,each group had 43 cases.The clinical symptoms,immunological findings and related imaging data of all cases were recorded.We investigated the relationship between the serum levels of anti-BRAF antibody and the clinical symptoms,immunological findings and related imaging changes of the RA group.Chi-square test,Mann-Whitney U test,Spearman's correlation test were used for statistical analysis.Results ① The serum levels of anti-BRAF antibody were significantly higher in RA group [A:1.093 (0.150-3.210)] than in other rheumatic diseases group [A:0.278 (0.093-0.455)] and healthy controls [A:0.202 (0.121-0.443)] (Z=-11.223,P=0.021 ; Z=-7.839,P=0.008 7).The serum levels of anti-BRAF antibody was not significantly different between patients with other rheumatic diseases group and healthy controls (Z=-0.905,P=0.445).② The difference in positive rate of rheumatoid factor (RF) in different groups of anti-BRAF antibody levels was statistically significant (λx2=10.14,P=0.006),namely the positive rate was significantlyhigher in the low-level group (88％) than in the high-level group (58％) (x2=10.03,P=0.002).Thepositive rate of anti-cyclic citrullinated peptide antibody and anti-keratin antibody of the three groups was not significantly different (x2=0.721,P=0.697; x2=1.735,P=0.188).③ There was significant negative correlation between the levels of anti-BRAF antibody and the course of disease (r=-0.233,P=0.047),RF(r=-2.84,P=0.039),IgM (r=-0.234,P=0.038),and hemoglobin (r=-0.287,P=0.032).There was no significant correlation between the level of anti-BRAF antibody and the joint swelling index (r=-0.133,P=0.230),joint pain index (r=-0.173,P=0.100),other clinical and laboratory parameters.There was significantly negative correlation between the ESR and hemoglobin level.④ There was no significant difference of the level of anti-BRAF antibody between the normal group and patients with bone destruction in the RA group (Z=-0.642,P=0.521).⑤ There was no significant difference in the level of anti-BRAF antibody between the normal group and patients with interstitial pulmonary fibrosis group in the RA group (Z=-0.121,P=0.904).Conclusion The anti-BRAF antibody can be used for the diagnosis of RA as a new autoantibody,and it can also be used in the diagnosis of early or RF-negative RA.There is no correlation between the level of anti-BRAF antibody and bone destruction and interstitial pulmonary fibrosis.

ObjectiveTo evaluate the application effect of clinical pathways in hospitalized inguinal hernia patients by using the Meta- analysis. MethodsPublished randomized controlled trials (RCT)in hospitalized inguinal hernia patients were searched and screened in CNKI、VIP、CBMdisc and Wanfang datebase under present standards.The quality of the included studies were evaluated and statistically analyzed by RevMan5.0 software. ResultsTotally 16 literatures including 1649 cases were eligible to the criteria (816 in clinical nursing pathways group and 833 in the traditional health education group)altogether.The Meta-analysis showed there were significant difference between the clinical pathway group and the traditional health education group in grasp of health knowledge,rate of patients,satisfaction degree,length of hospital stay and hospitalization expenses. ConclusionsClinical pathways is superior compared with the traditional health education for hospitalized inguinal hernia patients.

The emphasis and basic target of medical education is to cultivate medical students with high qualities. The qualities of medical students include professional ethics, self-cultivation, medical knowledge and physical-psychological quality, which is critical to the medical education. It has been proven that the teaching of Clinical Pathway is one of the most important factors to promote the improvement of comprehensive qualities of medical students.