BACKGROUND:There are many methods for dental impression disinfection, including ultraviolet disinfection method, spraying, immerses disinfection method, argon plasma jet and radio frequency glow discharge on the argon gas ionization disinfection method. At present, there is stil lack of studies addressing the disinfection effect after gargling with mouthwash.
OBJECTIVE:To detect the kil ing effect on bacteria and fungi from the dental impressions, which are taken after gargling with cetylpyridinium chloride gargle and stable chlorine dioxide gargle, respectively.
METHODS:One hundred patients were randomly divided into cetylpyridinium chloride gargle group and stable chlorine dioxide gargle group. They were told to gargle with clean water for 1 minute, and then impressions were taken to remove the models with sterile cotton swab sampling in neutralizing agent which were sent to laboratory for bacteria and fungi culture. After 1 hour, the patients were asked to gargle with the different mouthwashes for 1 minute again, and then sterile cotton swab sampling was done at the same position for bacteria and fungi culture. The number of colonies was recorded, and the kil ing effects on kil ing bacteria and fungi on the surface of the impressions before and after gargling with different mouthwashes were observed.
RESULTS AND CONCLUSION:The number of bacteria and fungi before gargal had no significance difference before gargling. After gargling, the number of bacteria and fungi on the model was significantly lower than before (P<0.001). Two groups did not appear with any adverse reactions, and patients felt refreshed and comfortable after gargling with mouthwash. To gargle with mouthwash before taking impressions can control the number of bacteria and fungi on the samples effectively, thereby achieving the aim of control ing the mutual infections between the doctor and the patients effectively.

Objective To evaluate the effect of rehabilitation training on rats with spinal cord injuries by Meta-analysis. Methods Ar-ticles were searched from PubMed( ~2013),CNKI(1989~2013), WanFang Data( ~2013),VIP(1989~2013),quality of included article was assessed with Jadad scale,and available data was analyzed with RevMan 5. 0 software. Results 287 related articles were identified,but only 11 eligible articles were included. The Meta-analysis of BBB score indicated that the rehabilitation training groups were better than con-trol groups. The BBB score[weighted mean difference(WMD) =1. 87,95%CI(1. 50,2. 33),Z=10. 02,P<0. 01]. There was significant diffence between two groups. Conclusion Rehabilitation training can improve the recover of hindlimb function.

Pre-testing preparation is the basis and starting point of genetic testing.The process includes collection of clinical information,formulation of testing scheme,genetic counseling before testing,and completion of informed consent and testing authorization.To effectively identify genetic diseases in clinics can greatly improve the diagnostic rate of next generation sequencing (NGS),thereby reducing medical cost and improving clinical efficacy.The analysis of NGS results relies,to a large extent,on the understanding of genotype-phenotype correlations,therefore it is particularly important to collect and evaluate clinical phenotypes and describe them in uniform standard terms.Different types of genetic diseases or mutations may require specific testing techniques,which can yield twice the result with half the effort.Pre-testing genetic counseling can help patients and their families to understand the significance of relevant genetic testing,formulate individualized testing strategies,and lay a foundation for follow-up.

Bioinformatic analysis and variant classification are the key components of high-throughput sequencing-based genetic diagnostic approach.This consensus is part of the effort to develop a standardized process for next generation sequencing (NGS)-based test for germline mutations underlying Mendelian disorders in China.The flow-chart,common software,key parameters of bioinformatics pipeline for data processing,annotation,storage and variant classification are reviewed,which is aimed to help improving and maintaining a high-quality process and obtaining consistent outcomes for NGS-based molecular diagnosis.