Objective To investigate the inhibitory effect and associated mechanism of rapamycin on proliferation and extracellular matrix (ECM) secretion in myofibroblasts stimulated by connective tissue growth factor (CTGF). Methods Primary cultivated myofibroblasts were divided into 6 groups: control, CTGF (100 μg/L), rapamycin 20 μg/L+CTGF 100 μg/L, rapamycin 40 μg/L +CTGF 100 μg/L, rapamycin 20 μg/L, and rapamycin 40 μg/L alone. 5'-bromodeoxyuridine (BrdU) incorporation assay was used to detect the myofibroblast proliferation.Western blot was used to analysis the secretory FN protein in the supernatant medium of cultured myofibroblasts and the ERK1/2 phosphorylation in myofibroblasts. Results CTGF (100 μg/L)incubation significantly increased the number of Brdu positive myofibroblasts(P<0.01) and the level of FN protein secretory (P<0.05) in cell supernatant medium compared with control group,respectively. The number of Brdu positive myofibroblasts markedly decreased by 62% and 70% (P <0.05) in rapamycin 20 μg/L+CTGF 100 μg/L and rapamycin 40 μg/L+CTGF 100 μg/L groups, respectively. The FN protein levels in supernatant were decreased by 15% and 44% compared with CTGF 100 μg/L group, respectively; but the difference of FN protein levels was significant only in rapamycin 40 μg/L group (P<0.05). CTGF could activate ERK1/2 at 10 minutes; but as myofibroblasts were pretreated with rapamycin 40 μg/L for 30 min, it abolished CTGF-induced ERK1/2 phosphoralation. PD98059, the specific inhibitor of ERK1/2, could block the effect of CTGF-induced proliferation (7%±5% vs 85%±7%, P<0.01) and FN secretion (1.0±0.1 vs 1.6±0.3, P<0.05). Conclusions Rapamycin partially suppresses the proliferation and ECM secretion of myofibroblasts induced by CTGF. Its effect may be through inhibiting CTGF-induced activation of ERKI/2 signaling pathway.

ABSTRACT:The seasonal characteristics of Japanese encephalitis (JE) in Gansu Province in 1962-2010 were analyzed in order to provide reference for making strategies of disease control and prevention .The JE data is the Epidemic Data between 1962 and 2003 from Gansu CDC ,and National Notifiable Diseases Registry System (NNDRS ,2004-2010) .Concentration ra‐tio and circular distribution method were used in this study .Results showed that the M‐value of the seasonal distribution of JE in 1962-2010 was 0 .825 9 .The corresponding peak date of JE was the 30th August (95% CI:22 July to 7 October) .The Z value of Rayleigh test was 3 506 .092 5 (P<0 .01) .In conclusion ,the concentration and circular distribution method helps us to grasp the JE epidemic peak season more accurately .There were significant seasona1 characteristics for JE in Gansu Province and the epidemic peak was from late July to early October .

Objective To observe the value of transvaginal color Doppler ultrasound (TVCDU) in the diagnosis of endo-metrial lesions.Methods All 246 patients suspected of endometrial diseases underwent TVCDU and transabdominal ultra-sonography.Ultrasonographic characteristics,especially the uterine endometrial thickness and blood flow distribution were observed,and the results were compared with surgery and pathological results.Results A total of 242 patients were pathologically diagnosed as endometrial diseases.TVCDU diagnosed 232 cases,with the diagnostic accordance rate of 95.87%.Transabdominal ultrasonography diagnosed 182 cases,with the diagnostic accordance rate of 75.21%.TVCDU was superior to transabdominal color Doppler examination (P<0.05).Conclusion TVCDU has high diagnosis accordance rate and can be used as routine examination of endometrial diseases.

In this paper, we analyse the general information of medical equipment clinical trials by clinical trial process management experience to elaborate the characteristics of the medical equipment clinical trials and the existent problems in our hospital in 10 years. We propose corresponding countermeasures to ensure the quality of medical tests, and improve the management of medical equipment clinical trials in hospital.
Clinical Trials as Topic ; Equipment and Supplies, Hospital ; Humans

Objective To explore the clinical characteristics of eosinophilic gastroenteritis. Methods The clinical data of 2 children with eosinophilic gastroenteritis was retrospectively analyzed. Results Eosinophilia was found in both cases and path-ological examination indicated eosinophil infiltration in intestinal mucosa. The clinical symptoms were improved after hypoaller-genic diet, amino acid formula and anti-allergy treatment. Conclusions Eosinophilic gastroenteritis is characterized by eosino-phil infiltration with unknown pathogenic factor leading the treatment of eosinophilic gastroenteritis lacking of sound evidence.

Objective To explore the relationships among social adaptation and the resilience,social support and coping style in undergraduate nursing students.Methods 758 students from two medical colleges in Shandong province were recruited by stratified random sampling method.They were assessed with China College Student Adjustment Scale (CCSAS),Resilience Scale for Adults (RSA),Social Support Rating Scale (SSRS),Simplified Coping Style Questionnaire (SCSQ).The data was analyzed with the structural equation mode.Results The social adaptation of undergraduate nursing students showed significantly positive association with the resilience,social support,positive coping style(r=0.113-0.607,P＜0.01),but the campus adaptation had negative association with negative coping style(r=-0.117,P＜0.01).The path analysis showed that the resilience,social support and coping style were the direct predictors of social adaptation (β=0.57,P＜0.05;β=0.0.26,P＜0.05;β=0.1,P＜ 0.05),and the resilience,active coping style played the mediating role between social support and social adaptation (β=0.31,P＜0.05;β=0.05,P＜0.05).Conclusion The social adaptation and the resilience,social support and active coping style of nursing undergraduates are closely related.

This paper introduces the Vest-style First-aid Kit which are newly developed for PLA.Adopting a vest structure the Vest-style First-aid Kit has the advantages of fully freeing both hands,and being fit for the ship confined space.Basic performance experiments,ergonomics tests and army tryout were performed and the results showed that the vest-style first-aid kit was scientifically designed,and easy to use,suiting the body,obviously saving physical energy.It has a reasonably designed interior medicine layout and can fulfill the requirements of first-aid operations in naval ships.

OBJECTIVE:To study the effective portion of the ultrafiltration membrane-separated ethanol extract of Pae-onia lactiflora and its antineoplastic activity in vitro. METHODS:The content of peoniflorin in ethanol extract of P.lactiflora was determined by HPLC. The drug-containing serum of mice was used to cultivate tumor cells such as HT29,HL60 and S180,then the inhibitory effect of P.lactiflora ultrafiltrate on the tumor cells and the association between dosage and administration time of the P.lactiflora ultrafiltrate were observed. RESULTS:P.lactiflora(10～100 g?kg-1) showed marked inhibition on all kinds of the above-mentioned tumor cells in a dose-dependent manner; the drug-containing serum also showed marked inhibition on all of kinds of tumor cells at different time in a time-dependent manner. CONCLUSION:The effective portion of ultrafiltration membrane-separated ethanol extract of P.lactiflora has antineoplastic activity in vitro.

Objective To study the effects of prokaryotic ubiquitin-like protein ( Pup)-proteasome system on the growth of Mycobacterium strains.Methods The genes encoding Pup ( pup gene) and protea-someβsubunit ( prcB gene) were respectively knocked out from Mycobacterium smegmatis ( M.sm) strains by homologous recombination.The growth and viability of the wild-type and mutant strains of M.sm were an-alyzed under normal culture condition and under hypoxia as well as anaerobic conditions.Results The pup and prcB genes were completely and precisely knocked out from M.sm strains and the mutant strains were named △SM-Pup and△SM-prcB, respectively.The△SM-Pup strains grew faster than the wild type ( WT) and△SM-prcB strains.No significantly differences in the growth of M.sm were found between the WT and△SM-prcB strains.Conclusion The Pup-proteasome system was involved in the growth of M.sm, espe-cially the pup gene.There was difference between pup and prcB genes in regulating the growth of M.sm.The functions and influences of Pup-proteasome system still need further investigation.

Background and purpose:Gene fusions have been identiifed as recurrent oncogenic events in lung adenocarcinoma. Our purpose are to study the histologic features of anaplastic lymphoma kinase (ALK), c-ros oncogene 1 receptor tyrosine kinase (ROS1) andRETproto-oncogene fusion-positive lung adenocarcinomas and to evaluate the correlation between psammoma bodies and fusion-positive lung adenocarcinomas.Methods:In this study, we performed a comprehensive histologic analysis of 44 fusion-positive (including 15RET, 20ALK and 9ROS1)
lung adenocarcinomas and 111 fusion-negative [including 20 epidermal growth factor receptor (EGFR), 20 Kirsten rat sarcoma viral oncogene (K-ras), 71 pan-negative] lung adenocarcinomas.Results:ALK,RET andROS1 fusion-positive lung adenocarcinomas were more prevalent in solid or acinar predominant adenocarcinoma. Multivariate analysis showed that tumors harboring a fusion gene had significantly higher prevalence of the presence of signet ring cells (P=0.000), micropapillary component (P=0.044), mucinous cribriform pattern (P=0.000) and extracellular mucin (P=0.010). The incidence of psammoma bodies was higher in the lung adenocarcinomas with a gene fusion than in tumors without gene fusions (P=0.000). Psammoma bodies were more likely to be found in tumors with any micropapillary component and/or mucinous cribriform pattern than in tumors lacking a micropapillary component and/or mucinous cribriform pattern (P=0.000).Conclusion:Our data showed that the presence of psammoma bodies, micropapillary component, mucinous cribriform pattern, extracellular mucin or signet ring cells may be either sensitive or speciifc to predict tumors harboring a fusion gene. These distinct morphologic features may be helpful in selecting cases for further accurate molecular testing.