Objective To investigate the expression of PES1 in ovarian cancer and its relationship with the vascular endothelial growth factor(VEGF) expression .Methods The expression of PES1 in ovarian cancer tissues and corresponding pericancerous tis-sues was detected with Western blot .Results The expression of PES1 in ovarian cancer tissues was obviously higher than that in the pericancerous tissues .The secretion amounts of VEGF in cell culture fluid of CAOV-3 and ES-2 with transfection of FLAG-PES1 were elevated from (178 .0 ± 11 .8) pg/mL and (309 .5 ± 18 .5) pg/mL to (375 .0 ± 18 .3) pg/mL and (633 .2 ± 25 .7)pg/mL respectively (P<0 .01) ,the secretion amounts of VEGF were also increased(P<0 .01) .The relative VEGF mRNA levels were also raised by 1 .8 times and 2 times respectively (P< 0 .01);Western blot simultaneously showed that the over expression of PES1 could increase the expression of HIF-1αin these two kinds of cells .The luciferase report gene transcriptional activation activity de-tection reveald that PES1 had no direct effect on the VEGF transcription .After cotransfecting HIF-1αSiRNA and FLAG-PES1 to CAOV-3 and ES-2 cells ,Western blot demonstrated that HIF-1αSiRNA could obviously inhibit the increase of HIF-1αexpression induced by PES1 ,at the same time the secretion amounts of VEGF and mRNA level were also supressed .Conclusion The expres-sion of PES1 is obviously up-regulated in ovarian cancer tissues .

Objective To assess the value of ultrasonography in the diagnosis of ovarian pregnancy (OP). Methods The characteristics of transvaginal ultrasound and color Doppler flow imaging of 38 cases of OP were reviewed, whose diagnoses were confirmed by operations and pathologic findings. Results Twenty eight cases(~73.7 %) were correctly diagnosed by ultrasound, nine cases(~23.7 %) were misdiagnosed either as lutein cysts or as tubal pregnancy, one case(~2.6 %) was missed by ultrasound examination. According to ultrasound findings, 24 cases(~63.2 %) were diagnosed as type of gestational sac, 14(~36.8 %) cases as type of complex mass. Conclusions Ultrasonography plays an important role in diagnosing OP, especially in earlier OP of the gestational sac type.

Female ; Infant, Newborn ; Humans ; Pregnancy ; Premature Birth ; Retrospective Studies ; Risk Factors ; Cerclage, Cervical ; Cervix Uteri