Objective:To identify the strain and to study the in-vitro susceptibilities of Candida species collected from clinical samples of skin and mucosa to Amphotericin B,Ketoconazole,Miconazole,Itraconazole,Fluconazole,5-Flucytosine and Terbinafin. Methods:110 clinical isolates of yeasts were identified by CHROMAGAR and API20C, meanwhile, the susceptibility to six antifungal drugs was examined by microdilution method using Sensititre Yeast One panels. Results:There were 94 C.albicans, 6 C.tropicalis,4 C.krusei,4 C.glabrata,and 2 C.parapsilosis among 110 isolates of yeasts.There were 0.91%,1.81%,18.28%,1.81%,5.45%,3.63% and 94.55% of isolates displaying resistance to Amphotericin B,Ketoconazole,Miconazole,Itraconazole,Fluconazole,5-Flucytosine and Terbinafin, respectively.Conclusions:C.albicans is the main infection strain in our hospital,and candida species display different resistance to six antifungal drugs. It is necessary to test strain identification and susceptibility of candida species to guild the rational clinical drug use.

Objective:To research the in vitro antifungal activity of 23 kinds of TCM against microsponrrn gypseum and trichophyton ncbrurn, the main causes of superficial dermatophyte. Methods:Semisolid agar dilution method was applied. Results:Some kinds of TCM showed strong antifungal activity against one or several kinds of fungi. Conclusion:Belamcandae, cuscuta seed,coptis chinensis,phellodendri and scutellaria are active in fungistasis in the extro-experiment.

Objective:To evalute the efficacy and safty of desloratadine against urticaria. Methods: Fifty patients with urticaria were randomly assigned to receive either 5mg of desloratadine daily or lOmg of loratadine daily for 7 days (acute urticaria) and 14 days (chronic urticaria). Results : There were no statistically significant differences between the two groups in terms of time in taking effect and clinical efficacy(P = 0.971 and P = 0.051). Adverse effects were similar and mild in the two groups. Conclusion :It is indicated that desloratadine is well - tolerated and effecitive for the treatment of urticaria.

Objective:To observe the antibacterial activity of different preparations of 10 kinds of Chinese traditional medicine to Taphylococcus aureus,Staphylococcus epidermidis,Bacillus coli,Bacillus typhosus and Bacillus aeruginosus in vitro-experiment. Methods:Liquid agar dilution method was used. Results:The antibacterial activity of coptis chinensis is the best,scutellaria is the second. The antibacterial activity of tincture is better than that of decoction. Conclusions:The antibacterial activity of coptis chinensis is best during the 10 kinds of Chinese traditional medicine,and that of tincture is the best during different preparations.

AIM: To investigate the effect of lowering intraocular pressure ( IOP ) and side effect of travoprost ( TA ) on phacoemulsification and intraoclular lens ( IOL ) implantation in primary open-angle glaucoma ( POAG) .
METHODS: Patients with POAG already received surgery of phacoemulsification and IOL implantation were selected by randomized, single - blind, parallel group trial. TA was applied once a day in 43 patients (43 eyes) of treatment group and brinzolamide was used twice in 43 patients ( 43 eyes ) of control group. All patients were observed for 12wk. IOP, ocular symptom and adverse reaction etc. were observed.
RESULTS: The daily average IOP ( mean ± standard deviation) in the treatment group decreased from (24. 20±3.01)mmHg (1mmHg=0.133kPa) to (16.77±2.89)mmHg and that in the control group was from ( 23. 87±3. 47 ) mmHg to ( 18. 81± 3. 07 ) mmHg. IOP pre- and pro-treatment within two groups had significant difference ( P<0. 01), IOP between the groups at each time point had statistical difference (P<0. 05). Conjunetival congestion increased and itching in treatment group was obviously more serious than that in the control group, but the treatment could continue. No other ocular and systemic adverse events related to the drugs were found.
CONCLUSION: It is demonstrated that travoprost is highly effective and safe in reducing IOP in POAG already received surgery of phacoemulsification and IOL implantation.

Glucocorticoids ; adverse effects ; therapeutic use ; Humans ; Respiratory Distress Syndrome, Adult ; drug therapy ; immunology ; metabolism ; Systemic Inflammatory Response Syndrome ; drug therapy ; immunology ; metabolism

Objective: To screen for the differentially expressed proteins during rat liver regeneration after partial hepatectomy (PH) by proteomics technique. Methods: Healthy male SD rats were randomly divided into 2 groups: hepatectomy group and sham operated group. The hepatectomy model was produced by 70% PH (n=35) and the sham operated rats (n=5) underwent the same surgical protocol without hepatectomy. Rats were executed at 2, 12, 24, 36, 48, 72 and 168 h after partial hepatectomy (each time 5 rats) and the right lobes were harvested. The total protein was extracted and analyzed by two-dimensional gel electrophoresis and mass spectrometric analysis. The differential proteins were then analyzed by Western blotting. Results: The spots of differential protein began to rise at 2 h after PH and peaked at 36 h after PH. A total of 78 protein spots were identified and 35 significant protein spots were found by mass spectrometric analysis. The 35 protein sports fell into 5 types according to their dynamic changes: 3 up-regulated and 2 down-regulated (with different regulation time periods and amplitudes); and their functions involved oxidative stress response, acute reaction, lipid and energy metabolism, intracellular signaling transduction, cell proliferation, etc., with some having unknown functions. Western blotting analysis showed that the prohibitin protein began to increase 2 h after PH and decreased to the normal levei after 48 h. Conclusion: It is indicated that many proteins and signal transduction pathways participate in the liver regeneration after partial hepatectomy.

Objective To investigate the effects of hydrogen-rich saline on postoperative cognitive dysfunction (POCD) in aged mice and the possible mechanism.Methods Seventy-two male C57BL/6 mice,aged 18 months,weighing 45-55 g,were randomly divided into 4 groups (n =18 each):control group (group C),hydrogen-rich saline group (group H),POCD group and POCD + hydrogen-rich saline group (group PH).POCD was induced by open tibial fracture.Hydrogen-rich saline 10 ml/kg was injected intraperitoneally twice a day for 7 days before surgery.Six mice were chosen at day 1,3 and 7 after surgery and cognitive function was assessed by Morris water maze test.The mice were then sacrificed and hippocampus was isolated for determination of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) contents (by ELISA) and nuclear factor-κB (NF-κB) activity.Results Compared with group C,the escape latency was significantly prolonged,the time of staying at the original platform quadrant was shortened,the frequency of crossing the original platform was decreased at 3 and 7 days after surgery,and the contents of NF-α and IL-1β and NF-κB activity were increased during 1,3 and 7 days after surgery in group POCD (P ＜ 0.05),and no significant change was found in the parameters mentioned above in group H (P ＞ 0.05).Compared with group POCD,the escape latency was significantly shortened,the time of staying at the original platform quadrant was prolonged,the frequency of crossing the original platform was increased at 3 and 7 days after surgery,and the contents of NF-α and IL-1β and NF-κB activity were decreased at 1,3 and 7 days after surgery.Conclusion Hydrogen-rich saline can alleviate POCD via inhibiting NK-κB activity in hippocampus and reducing inflammatory response.

Objective To evaluate endos copy combined with peritoneal lavage for the treatment of severe acute biliogen ic pancreatitis(SAP).Methods 62 cas es of SAP were randomized into endoscopic intervention group (32 cases) and con trol group (30 cases) treated conservatively. The endoscopic group were treated with ERCP and EST,and BUS guided abdominal paracentesis drainage and antibiotic lavage. Abdomi nal pain, amylase level in blood and urine, WBC counts, average hospital stay, t he operation rate within one month, complication, mortality and curative rate in patients of the two groups were recorded and compared.Results Results in endoscopic group were superior to contro l group (all P

The aim of this article is to explore the dual-system of German geriatric nursing, curriculum of geriatric nursing, concept of major and evaluation mode, which could provide references and reflection for national geriatric nursing development.