Objective To investigate the expression and prognostic significance of inhibitor of growth 4 (ING4) and tail-type homeobox transcription factor 2 (CDX2) in colorectal cancer. Methods The expressions of ING4 and CDX2 pro-teins were detected by immunohistochemistry in 99 tissue samples of colorectal cancer and 30 corresponding para-cancer-ous normal tissue samples. The data of clinic outcomes were collected. The correlations between the expressions of ING 4 and CDX2 and clinicopathological parameters were also analyzed. Results The positive expression rates of ING4 and CDX2 were 68.8%and 72.7%in colorectal cancer tissues, which were significantly lower than those of corresponding normal tissue samples (93.3% and 96.7%, P<0.05). There were significant differences in the differentiation, depth of invasion, lymph node metastasis and tumor stage between expressions of ING4 and CDX2 (P<0.05). The 5-year survival rate was significant-ly lower in ING4 negative group (35.5%) compared with that of ING4 positive group (77.9%). The 5-year survival rate was significantly lower in CDX2 negative group (48.1%) than that of CDX2 positive group (70.8%, P<0.05). The expression of ING4 was positively correlated with the expression of CDX2 in colorectal cancer. Conclusion The expressions of ING4 and CDX2 are strongly associated with the carcinogenesis, development and prognosis of the colorectal cancer,which suggests that ING4 and CDX2 might be used as prognostic markers for the colorectal cancer.

Objective To investigate the role of HER2 and Ki-67 in colorectal cancer pathogenesis and their influence on the prognosis in the patients with colorectal cancer.Methods The expressions of HER2 and Ki-67 proteins in colorectal cancer tissues from 71 cases of colorectal cancer and 30 cases of para-cancerous normal tissues were detected by usingthe immunohistochemistry.The expressions of HER2,Ki-67 and their clinicopafhological parameters and prognostic significance were also analyzed.Results The positive expression rate of HER2 in colorectal cancer tissue (32.4％) was higher than that in para-cancerous normal tissues (10.0％);the positive expression rate ofKi-67 in colorectal cancer tissue (85.9％) was significantly higher than that in para-cancerous normal tissues (6.7 ％),the difference was statistically significant (P＜0.05).The expression of HER2 in colorectal cancer tissues was correlated with tumor differentiation degree,lymph node metastasis,number of lymph node metastasis and TNM staging (P＜0.05).The expression of Ki-67 in colorectal cancer tissues was correlated with infiltration depth,lymph node metastasis,number of lymph node metastasis and TNM staging (P＜0.05).HER2 in colorectal cancer tissues was positively correlated with Ki-67 (r=0.515,P=0.001).The 5-year disease-free survival rates in the HER2 positive group and the HER2 negative group were 48.4％ and 80.8％,the difference was statistically significant (P＜0.05).The 5-year diseasefree survival rates in the Ki-67 high expression group and the Ki-67 low expression group were 56.5 ％ and 85.5％,the difference was statistically significant (P＜0.05).The 5-year survival rate in the HER2 positive group and the HER2 negative group were 60.9 ％ and 85.5 ％,the difference was statistically significant (P＜0.05).The 5-year survival rate in the Ki-67 high expression group and the Ki-67 low expression group were 63.9％ and 85.5 ％,the difference was statistically significant (P＜0.05).The single factor analysis showed that tumor differentiation degree,infiltration depth,lymph node metastasis,number of lymph node metastasis,TNM staging,the expressions of HER2 and Ki-67 were related with prognosis in the patients with colorectal cancer (P＜0.05).The Cox multivariate analysis results showed that tumor infiltration depth and TMN staging were the independent factors affecting prognosis in the patients with colorectal cancer.Conclusion The expressions of HER2 and Ki-67 are associated with the development of colorectal cancer.The 5-year disease-free survival rate and the 5-year survival rate in the patients with HER2 and Ki-67 positive become significantly decreased.

Objective To explore the characteristics of response to joint attention(RJA)under diverse guiding behaviors for pre-schoolers with moderate to severe autism spectrum disorder(ASD). Methods From March to May,2023,21 children with moderate to severe ASD and 16 children with developmental de-lays matched the physiological ages were selected from Jiaxing Sunlight Rehabilitation Kindergarten,and 16 typ-ical developmental children matched the physiological ages were selected from the kindergartens nearby.They accepted a behavioral experiment on RJA.The number of RJA,frequence of RJA and the coefficient of variation of guiding behaviors needed to RJA were compared among the three groups. Results About half of the ASD group responded after guiding of head-turning,and the others required higher levels of guiding.The frequence of RJA after guiding of head-turning was less in the ASD group than in the typical devel-opment group(χ2>6.170,P<0.05),and the coefficient of variation of guiding behaviors was more(d=4.039,P<0.001). Conclusion Preschoolers with moderate to severe ASD are able to respond to joint attention,and this ability is poorer than typically developing children.The guiding behavior of the evaluator should be considered in assessing and intervening RJA in preschoolers with ASD.

Objective : To investigate the effects of SETDB1 on the proliferation and apoptosis of human colon cancer cells. Methods : The senescence of colon cancer cells was induced by doxorubicin, and the protein expression of SETDB1 in the senescent cells was detected by Western blot.Colon cancer cell models with low expression of SETDB1 and overexpression of SETDB1 were constructed by using shRNA(shSETDB1#1 and shSETDB1#2) of SETDB1 and overexpression plasmid, respectively.Western blot was used to detect the expression of SETDB1,the CCK8 assay was performed to detect cell proliferation while a Senescence β-Galactosidase Staining Kit was used to detect senescent cells, and Western blot was used to detect the changes of p53 and p21.The reverse experiment was carried out with p53 overexpression plasmid. Results : Cell senescence evoked by doxorubicin could down-regulate the protein expression of SETDB1.After low expression of SETDB1,the proliferation ability of colonic cancer cells decreased, and the proportion of senescent cells increased from(22.00±4.35)% to(54.00±5.56)% and(53.33±4.93)%(P<0.001).After overexpression of STEDB1,the proliferation ability of colon cancer cells increased, and the ratio of senescent cells decreased from(43.33±6.11)% to(21.33±3.51)%(P<0.01).Through GSE56496 enrichment and analysis, it was found that SETDB1 was related to p53 signal pathway, silencing SETDB1 could increase the levels of p53 and p21 protein, while overexpression of SETDB1 could decrease the level of p53 and p21 protein, and overexpression of p53 reversed the decrease of cell senescence caused by overexpression of SETDB1. Conclusion SETDB1 inhibits the senescence of colon cancer cells by inhibiting p53/p21 signal pathway, which provides a potential target for individualized tumor therapy.

Objective To investigate the therapeutic effect of Taohong Siwu decoction on a mouse model of carbon tetrachloride (CCl 4 )-induced liver fibrosis and its mechanism of action. Methods A total of 24 male C57BL/6 mice were randomly divided into normal group, model group, and Taohong Siwu decoction group, with 8 mice in each group. The mice in the model group and the Taohong Siwu decoction group were given intraperitoneal injection of 10% CCl 4 , and Taohong Siwu decoction was given by gavage since week 3 for 4 consecutive weeks. Liver function [alanine aminotransferase (ALT) and aspartate aminotransferase (AST)] was measured, and liver pathomorphology was observed. Real-time PCR was used to measure the mRNA expression of α-smooth muscle actin (α-SMA), hyaluronic acid synthase-2 (HAS-2), and collagen type Ⅰ(Col1), and Western blotting was used to measure the protein expression of α-SMA, Col1, and HAS-2. Primary hepatic stellate cells (HSCs) were isolated, and HAS-2 was silenced by siRNA to observe its influence on HSC activation. The t -test was used for comparison of continuous data between two groups; a one-way analysis of variance was used for comparison between multiple groups, and the SNK or least significant difference t -test was used for further comparison between two groups. Results Compared with the normal group, the model group had significant increases in serum liver function parameters (ALT, AST) and the Taohong Siwu decoction group had significant reductions in the serum levels of ALT and AST (all P < 0.01). Pathological staining showed that the model group had marked inflammatory cell infiltration and formation of fibrous septa by proliferated collagen fibers, and the Taohong Siwu decoction group had loose fibrous septa and alleviated inflammatory cell infiltration. Compared with the model group, the Taohong Siwu decoction group had significant reductions in the mRNA and protein expression of α-SMA and Col1(all P < 0.001). Compared with the normal group, the model group had a significant increase in the mRNA expression level of HAS-2 in liver tissue ( t =6.14, P < 0.05), and compared with the model group, the Taohong Siwu decoction group had a significant reduction in the protein expression level of HAS-2 (0.29±0.10 vs 1.00±0.12, t =70.73, P < 0.001). After HAS-2 was silenced by siRNA, the Si HAS-2+transforming growth factor β (TGFβ) group (treated with TGFβ) had significant reductions in the mRNA expression levels of α-SMA and Col-Ⅰ compared with the NC+TGFβ group ( P < 0.01). Conclusion Taohong Siwu decoction exerts a marked therapeutic effect on CCl 4 -induced liver fibrosis in mice by inhibiting HAS-2.