OBJECTIVE:To explore the effect of different plasmapheresis supplement timing on therapeutic efficacy of toxic liver injury. METHODS:96 patients with toxic liver injury and divided into group A,B ,C and D with 32 cases in each group ac-cording to different plasmapheresis supplement timing. All patients received plasmapheresis supplement based on routine treatment. In group A,synchronized fluid replacement was 100% fresh plasma;in group B,synchronized fluid replacement was 40% normal saline firstly,and then 60%fresh plasma;in group C,substitute liquid was given till the in vitro blood reached 12%of circulation amount,supplement order as 40% normal saline for the first supplement,and then add 60% fresh plasma. The clinical symptoms and signs,liver function,prothrombin activity and blood biochemical indicators were observed in each group before and after treat-ment. RESULTS:Plasmapheresis supplement had good therapeutic efficacy on toxic liver injury;but the time of clinical symptoms and signs disappearance in group C was significantly shorter than that in group A and B,and the group B was significantly shorter the group A,with statistical significance (P<0.05). The plasma bilirubin,prothrombin activity,albumin levels of 3 groups after treatment were significantly better than before,with statistical significance(P<0.05);the group C was better than the group A and B,and the group B was better than the group A,with statistical significance(P<0.05). Compared with before treatment,the lev-els of AST and ALT in 3 groups decreased significantly after treatment,with statistical significance(P<0.05);the group B and C were better than the group A,with statistical significance(P<0.05);the group B and C was similar to each other,without statisti-cal significance(P>0.05). CONCLUSIONS:Different plasmapheresis supplement timing have different effect on toxic liver injury. The supplement method that giving 40% normal saline for the first supplement,and then add 60% fresh plasma when in vitro blood reach 12%of circulation amount has more significant effect.

Objective To study the changes of serum and tissue soluble interleukin-2 receptor (slL-2R) and soluble intercellular adhesion moleculer-1 (sICAM-1) in eolorectal cancer and their clinical significance. Methods Serum levels of sIL-2R and sICAM-1 were detected by the double antibody sandwich ELISA method in 44 colorectal cancer patients before and after operation, 28 polyp intestinal and 30 controls. Simultaneously, tissue levels of them were measured in co]oreetal cancer and polyp intestinal. Results The serum sIL-2R[(209.27±127.42) pmol/L] and sICAM-1[(693.22±276.25) ng/ml] in coiorectal cancer were significantly higher than those in the polyp intestinal and controls.The tissue sIL-2R [(233.66±170.22)pmol/L] and sICAM-l[(706.92±286.09) ng/ml] in colorectal cancer were significantly higher than those in the polyp intestinal. The serum and tissue levels of sIL-2R and sICAM-1 in colorectal cancer had relationships with Duke stages and no relationships with the histological differentiation. The levels of serum sIL-2R and sICAM-1 declined remarkably after one month of radical operation, but it decreased illegibly after palliative operation. Conclusion Alterations of serum and tissue sIL-2R and sICAM-1 levels may be used as indicators of diagnosis, choice of operative method, judgement of prognosis in eoloreetal cancer.

Objective:To observe the therapeutic effects of auricular point sticking in managing functional dyspepsia.Methods:Seventy patients with functional dyspepsia were randomly divided into two groups,with 35 patients in the treatment group receiving auricular point sticking therapy,and the other 35 patients in the control group managed by taking Domperidone orally.The therapeutic effects were evaluated 4 weeks later.Result:The total effective rate was 85.7% in the treatment group and82.9% in the control group.Although the difference of the total effective rate between the two groups was not significant,the treatment group was superior to the control group in the improvement of several symptoms(P<0.05).Conclusion:Auricular point sticking therapy is effective in treatment of functional dyspepsia,and compared to Domperidone,it is better in managing gastric pain,abdominal bloating,and belching.

Objective To investigate whether the proliferation of human hepatoma cell can be induced by insulin and which can be inhibited by Octreotide. Methods A human hepatoma cell line BEL7402 was used in this study. Using MTT method, cell count and Flow Cytometry (FCM), the effects of insulin and/or Octreotide on the number of viable cell, its ratio, the total number of cells, cell cycle and proliferative index (PI) in phasesynchronized cells were assayed. Results The cell number, the viable cell number and its ratio in insulin (0～5 ?g/ml)treated BEL7402 cells increased, while both in basic and insulininduced ones all decreased when Octreotide 0～2 ?g/ml was used, both in dosedependent manner. 1 ?g/ml of Octreotide resulted in a reduction of PI which was increased by 5 ?g/ml of insulin. Moreover, a S cell cycle block was detectable in phasesynchronised BEL7402 cell treated with 1 ?g/ml of Octreotide. Conclusion In vitro, proliferation of human hepatoma cell can be induced by insulin, while Octreotide exerted an inhibitory effects on basic and insulininduced growth of human hepatoma cell.

Objective To investigate the association of ILK expression in human renal interstitium and the pathological injury of renal interstitium in the patients with chronic nephropathy.Methods The expressions of ILK,TGF?1,E-cadherin and fibronectin(FN) in renal tissue were investigated with immunohistochemistry and computer image analysis system in 49 patients with chronic kidney disease.According to the score of the renal interstitial pathologic injury,49 patients were divided into four groups: 4 in no injury group(the normal control group),17 in slight injury group,16 in mild injury group,12 in severe injury group.Results ILK was hardly detected in normal control group,and ILK was observed in tubulointerstitial area in 3 injury groups.The expression of ILK was prominent in tubular epithelial cells,next in the damaged tubulointerstitial area.A significant positive correlation was found between the expression of ILK in renal interstitial tissue and the degree of renal tubulointerstitial pathological injury(r=0.736),as well as that of TGF?1(P

Objective To analyze the differential expressions of miR-146a,miR-206,miR-223 and let-7c-1,such as cell differentiation-related miRNAs,in CXCR4-positive and CXCR4-negative subsets of the Lewis lung cancer cell lines(LLC).Methods CXCR4-positive and CXCR4-negative subsets were isolated from LLC by immunomagnetic beads sorting,and then their total cellular RNA were extracted by Trizol,expression of 4 miRNAs were detected by real-time fluorescence quantitative PCR(TaqMan probe),and potential target genes of miRNA whose differential expression was the most significant were predicted.Immunohistochemistry was carried out to confirm differential expression of the key molecule of certain research value within CXCR4-positive and CXCR4-negative subsets growing tumor tissue,and a BLAST search was performed to identify homologies of its 3′UTR.Results Compared to CXCR4-negative subsets,the expression of 4 miRNAs were lower in CXCR4-positive subsets,and expression of miR-223 had the most significant difference(Fold change=8.26).By softwares forecasting,miR-223 had potential target sites of IGF1R,IGFBP5,Pik3cb,ELK-1 and E2F1 mRNA,such as key molecular of IGF1R signaling pathway.The expression of IGF1R of CXCR4-positive subsets growing tumor tissue was significantly higher than that of CXCR4-negative subsets.Conclusion miR-223 is lowly expressed in CXCR4 positive cells from Lewis lung carcinoma cell lines.Position 238～244 nt and 688～695 nt in target sequences of 3′UTR of IGF1R mRNA was highly homologous by screening.Close correlation is found between miR-223 and IGF1R signaling pathway.The mechanisms underlying this biologically important finding need to be further explored.

0.05).Tumor formation was found in subgroups of positive CXCR4 cells(7 ? 103 ).Negative and positive CXCR cells were inoculated into 3 mice respectively at the concentration of 5 ? 105 and 2 ? 104 cells.No metastasis occurred in the former group.However,lung metastasis was found in 2 mice and ear metastasis was observed in 1mouse of the latter group.Conclusion Subgroups of positive CXCR4 cells in LLC are characterized by certain properties of cancer metastatic stem cells and have the ability to renew themselves and metabolize.

Objective To cultivate,identify and sort bronchoalveolar stem cells(BASC)derived from normal adult mouse lung.Methods After enzymatic digestion of lung tissue with dispase and collagenase in combination,the Sca-1+ cells were isolated from the obtained pulmonary cells by magnetic cell sorting.These Sca-1+ cells were cultured in dishes coated with collagen and mouse fibroblast cell line Swiss-3T3 under a serum-free culture system for BASC,which were identified by the dual-color immunofluorescent staining clara cell specific antigen(CCA)and surfactant protein C(SP-C).Finally,these pure BASC were isolated by the flow cytometry.Results One lung of normal adult mouse could yield(1.6-1.8)?107 nucleated cells in this enzyme digestion procedure.The percentage of Sca-1+ cells we sorted from lung tissue was much higher than the unsorted [(87.3?5.9)% and(9.6?1.8)%,P

Objective:To investigate the expression of VEGF-C in human lung carcinoma cell A549 induced by hyaluronan(HA) in vitro. Methods:A549 cells cultured in vitro were divided into three groups: control group(C): cultured with free serum medium(FSM);HA treatment group 1(HA1) and group 2(HA2): cultured with FSM containing different concentration of HA(HA1 group: 10?g/ml,HA2 group: 20?g/ml).After 48 hours,VEGF-C expression were examined by reverse transcription-polymerase chain reaction(RT-PCR)? Western blot and immunofluorescence staining. Results:Group C: both VEGF-C mRNA and protein expression were weak positive.HA1 and HA2 groups: both VEGF-C mRNA and protein expression were strong positive and increased significantly in a HA dose-depended way(P

Objective: To investagate the antioxidative action of aloe and its dose-effect relationship. Methods:Sprague-Dawley rats were killed and then the livers were removed to isolate the microsome which can generate the reactive oxygen species in the presence of VC and Fe2+ or cumene hydroperoxite(CHP). In these peroxidative damage models, different dosages of aloe extract were added. Then the contents of malondialdehyde (MDA) were examined for analyzing the antioxidative action of aloe extract. Results:In CHP model, the content of MDA in those groups with different dosages of aloe extract decreased significantly (P