Objective To explore NF-κB(P65)expression in breast cancer and its clinical significance.Methods Immunohistochemical staining was used to examine the expression of NF-κB(P65)and its relationship with related elinicopathological factors were analyzed in eighty-seven IDC(invasive ductal cancinoma).Results NFκB(P65)expression was significantly correlated with lower tumor grading,maxilary lymph node metastasis,later TNM styng and HER-2 positive(all P＜0.05),but not with tumor size,age of the patient and the state of ER、PR.Conclusion NF-κB(P65)should be risk factors of poor prognosis in IDC.

Objective To study the effect of ecdysterone (EDS) on rabbits chondrocytes that is injuried by lipopolysac?charide (LPS). Methods Aricular chondrocytes were isolated from rabbits and randomly divided into three groups:control group;chondrocytes with LPS induced injury (LPS group);injury chondrocytes treated with EDS (LPS+EDS group). The cell proliferation and cell apoptosis of chondrocytes were determined by MTT method and flow cytometry assay respectively. The mRNA and protein expression levels of inducible nitric oxide synthase (iNOS) were detected by RT-PCR and western blot. In addition, the content of NO and IL-1βwere measured by nitric acid reductase assay and enzyme-linked immunosorbent assay (ELISA) respectively. Results Attenuated proliferation, increased cell apoptosis, iNOS, NO and IL-1βwere seen in LPS group , but all these changes were significantly reversed by EDS treatment (P<0.05). Conclusion Ecdysterone exhib?ited a protective effect on LPS induced rabbits chondrocytes injury through inhibiting the expression of iNOS.

Acquired Immunodeficiency Syndrome ; prevention & control ; Adolescent ; Adult ; Behavioral Risk Factor Surveillance System ; China ; Cross-Sectional Studies ; Dangerous Behavior ; Female ; Humans ; Middle Aged ; Sex Work ; Young Adult

Objective To improve the diagnosis of cytomegalovirus pneumonia in patients who have undergone kidney transplantation. Methods The CT appearances of 10 cases were retrospectively analyzed. Results Ground glass opacity was seen in all cases, and disseminated several lobes were seen in 7 cases, diffuse distribution was seen in 3 cases. In addition,lung fibrosis happened in 3 cases,nodule in 2 cases,and consolidation in 3 cases.Conclusion Multi-slice CT could provide very valuable information for early diagnosis of cytomegalovirus pneumonia.

Objective The purpose of this study was to prepare RGD4C modified ferritin nanocages (RGD4C-FRT) for targeted drug delivery to rat hepatic stellate cells (HSC-T6). Methods RGD4C modified human H-chain ferritin was expressed and purified. Doxorubicin (Dox) was encapsulated into the cavity of RGD4C-FRT through ion channels, which resulted in RGD4C-FRT-Dox. The target of RGD4C-FRT-Dox to HSC-T6 was detected using fluorescence microscopy. Results Transmission electron microscopy showed that RGD4C-FRT was hollow spherical-structured with uniform size and good dispersion. The average particle diameters of RGD4C-FRT and RGD4C-FRT-Dox were 12.57 nm and 20.13 nm , respectively. The drug encapsulation efficiency and loading percentage of RGD4C-FRT-Dox were 77.32% and 15.88% respectively. RGD4C-FRT-Dox was significantly uptaken by HSC-T6, and the uptake could be blocked by the empty carrier RGD4C-FRT. Conclusion RGD4C-FRT-Dox can specifically target HSC-T6. Further animal experiments are needed to inspect its therapeutic effect on liver fibrosis.

OBJECTIVETo explore the impact of measurement error on the associated effects under the incorrectly-measured variables when mixed with precisely measured variables.

METHODSBased on the functions of measurement error, correlation of incorrectly-measured predictors and precisely measured explanatory variables, number of precisely measured explanatory variables and associated effect, the 'R Project for Statistical Computing' method is used to analyze the impact of measurement on the validity of a study.

RESULTSUnder the scenario that the continuous response Y and the continuous explanatory Z are precisely measured but the continuous predictor X is incorrectly-measured, when focusing on inference about the effect of X on Y, the non-differential measurement error always makes the value of estimated effect less than the actual value, and the attenuation effect of measurement error more closely worsens the correlation of X and Z. Under a misclassification dichotomous predictor X with an additional precisely measured explanatory variable Z and focusing on inference about the effect of X on Y, the misclassification bias is not only related to the sensitivity and specificity of exposure measurement, but also to the correlation between X and Z and exposure proportion of X. The attenuation factor (AF) decreases gradually with the increasing correlation between X and Z. For instance, in the p = 0.5 scenario, AF is 1.419, and the estimated effect of dichotomous predictor X on continuous response Y is more than the actual effect. When it increases to 0.9, AF is 0.474, the estimated effect becomes less than the true effect.

CONCLUSIONIn the studies of the impact of measurement error in linear regression with additional precisely measured explanatory variables, the impact of measurement error on the associated effect is relatively complex, suggesting that it is necessary to control and to assess the measurement error bias in order to correctly interpret the results of a study.

Bias ; Epidemiologic Research Design ; Epidemiologic Studies ; Linear Models ; Models, Statistical

OBJECTIVETo compare the therapeutic effects of routine acupuncture, the electroacupuncture and the combined therapy of electroacupuncture and acupoint injection.

METHODSNinety-one cases were randomly divided into a routine acupuncture group (30 cases), an electroacupuncture group (31 cases), and a combined therapy of electroacupuncture and acupoint injection group (30 cases). Zusanli (ST 36), Fengchi (GB 20), Anmian (Extra), Taiyang (EX-HN 5), Hegu (LI 4), Yintang (EX-HN 3), Baihui (GV 20) and Sishengcong (EX-HN 1) were selected among 3 groups. Even manipulation was applied in routine acupuncture group; G 6805 electroacupuncture apparatus was added in electroacupuncture group; in combined therapy of electroacupuncture and acupoint injection group, electroacupuncture was applied, besides, Vitamin B12 0.5 mg and 0.2%/ Lidocaine 2 mL were injected at Fengchi (GB 20) and Anmian (Extra). Twenty treatments were given in 4 weeks. The changes of average blood flow of vertebral artery and basilar artery before and after treatment were observed and graded by the cervical vertigo syndrome and function score; the therapeutic effects were evaluated as well.

RESULTSThe average blood flow of vertebral artery and basilar artery, and the cervical vertigo syndrome and function score were improved in 3 groups (all P < 0.01), in which, it was more obvious in combined therapy of electroacupuncture and acupoint injection group than in others (P < 0.05, P < 0.01), and it in electroacupuncture group was superior to that in routine acupuncture group (P < 0.05). The effective rate was 63.3% (19/30) in routine acupuncture group, 80.6% (25/31) in electroacupuncture group and 90.3% (28/30) in combined therapy of electroacupuncture and acupoint injection group, indicating the significant differences among them (P < 0.05, P < 0.01).

CONCLUSIONThe routine acupuncture, electroacupuncture, and combined therapy of electroacupuncture and acupoint injection are effective for cervical vertigo; the combined therapy is the best, and electroacupuncture comes second. It illustrates that the routine acupuncture, electroacupuncture, and combined therapy of electroacupuncture and acupoint injection have additive effects on treatment of cervical vertigo.

Acupuncture Points ; Aged ; Electroacupuncture ; Female ; Humans ; Injections ; Lidocaine ; administration & dosage ; Middle Aged ; Regional Blood Flow ; drug effects ; Treatment Outcome ; Vertebral Artery ; drug effects ; physiopathology ; Vertigo ; drug therapy ; physiopathology ; therapy ; Vitamin B 12 ; administration & dosage

OBJECTIVE:To observe clinical efficacy and safety of safflower yellow pigment in the treatment of elderly pa-tients with diabetic nephropathy complicated with acute myocardial infarction. METHODS:A total of 102 elderly patients with dia-betic nephropathy complicated with acute myocardial infarction were selected from our hospital during Jan. 2013-Jun. 2015,and then divided into observation group(52 cases)and control group(50 cases)according to random number table. Control group was given routine treatment as anticoagulation,regulating blood lipid,controlling blood glucose and blood pressure. Observation group was additionally given Safflower yellow pigment for injection 150 mg added into 0.9% Sodium chloride injection 250 mL,ivgtt, qd,on the basis of control group. Both groups received treatment for 2 weeks. Clinical efficacies of 2 groups were observed. Renal function indexes(UMA,Scr,BUN,TC,TG),cardiac function indexes(EF,SV,CO,CI),inflammatory factors(CRP,IL-6, TNF-α)and hemorheological indexes(whole blood high-shear viscosity,whole blood low-shear viscosity,hematocrit,fibrinogen and platelet aggregation rate)were compared between 2 groups before and after treatment. The occurrence of ADR was recorded in 2 groups. RESULTS:Total response rate of observation group was 96.15%,which was significantly higher than 88.00% of control group,with statistical significance(P<0.05).Before treatment,there was no statistical significance in renal function indexes,cardiac function indexes,inflammatory factors or hemorheological indexes between 2 groups(P>0.05).After treatment,renal function index-es,inflammatory factors and hemorheological indexes of 2 groups were decreased significantly,while cardiac function indexes were in-creased significantly;above indicators of the observation group was significantly better than control group,with statistical significance (P<0.05).No obvious ADR was found in 2 groups.CONCLUSIONS:Safflower yellow pigment shows significant therapeutic effica-cy for elderly patients with diabetic nephropathy complicated with acute myocardial infarction,can significantly improve renal function and cardiac function,decrease inflammatory factor levels and improve hemorheological indexes with good safety.

Objective To investigate the breeding conditions of mosquitoes and larvaes in 12 schools in Xuhui District of Shanghai before and after the beginning of summer term, and to provide basis for mosquitoes control in schools. Methods We used stratified random sampling to select 4 schools from each of the categories: kindergartens, primary and secondary schools, colleges and technical secondary schools in Xuhui District in August and September 2019.We used the methods of human landing catch, visual evaluation and ladling to investigate the breeding conditions of mosquitoes and larvaes before and after the beginning of summer semester. Results The mosquito density was 10.50 (per person per time) after the beginning of term, lower than 19.00 (per person per time) before schools opening, and the difference was statistically significant (χ²=4.20, P < 0.05).The positive rate of larvae breeding in small-scale ponds after the beginning of term was 31.47%, lower than 44.23% before the schools opening, and the difference was statistically significant (χ²=4.22, P < 0.05).The positive rate of larval breeding in medium and large static water was 14.00% after the beginning of term, lower than 17.86% before the schools opening, and the difference was no significant (χ²=1.03, P=0.31). Conclusion Schools need to take the following actions: to focus on the work to manage mosquito breeding sites on campus before the beginning of the term; to strengthen the daily work of environment control; to establish a model of prevention and control in cooperation with multi-medical and educational departments, and to form a collaboration system in application of drugs to rain-water catch basin sand in other mosquito control work in residential areas.

OBJECTIVETo observe clinical therapeutic effect of moxibustion on abnormal blood lipids.

METHODSThe patients who did not take the medicine for regulating blood lipids and had still abnormal blood lipids after diet therapy for 3 months, were divided into 4 groups according to different types of abnormal blood lipids. Forty cases selected in each group were again divided randomly into a treatment group and a control group. The treatment group were treated with moxibustion at Shousanli (LI 10), Zusanli (ST 36) and Shenque (CV 3) on the basis of diet therapy, and the control group only with the diet therapy. Their therapeutic effects were observed and compared after treatment of 90 days.

RESULTSThere were significant differences between the treatment group and the control group in various groups of different types of abnormal blood lipids (P < 0.05).

CONCLUSIONMoxibustion at Shousanli (LI 10), Zusanli (ST 36) and Shenque (CV 3) has a better therapeutic effect on abnormal blood lipids.

Acupuncture Points ; Biomedical Research ; Humans ; Lipids ; blood ; Medicine, Chinese Traditional ; Moxibustion