Objective To study the ZT glue postpone iodinated oil metabolism in liver. Methods We had intubated into right portal veins for 5 rabbits, injected 131 iodine iodinated oil and ZT glue, successively recorded ? counts of liver region by ? calibration equipment, created local 131 iodine iodinated oil metabolism equation, calculated effective half drained times, and finally observed the liver and lung in pathology. Another 5 rabbits had been merely injected with 131 iodine iodinated oil as the control group. Results Experimental 131 iodine iodinated oil slow group was higher in proportion than the control group, with delaying of effective half drained times. 131 iodine iodinated oil had been retained more proportion within right liver causing damage to liver tissue. Conclusions ZT glue can postpone iodinated oil metabolism in liver.

As a possible pathogen of blepharitis,Demodex is gradually known by ophthalmologists.Demodex is one of the permanent small parasitic mites in human hair follicle and sebaceous glands,with high infective rate in human body.There are two kinds of Demodex that colonized in human eyelid:Demodex folliculorum and Demodex brevis.The life cycle of Demodex is about 14.5 days.There have been different views on Demodex as the cause of blepharitis.We performed a deep research on the pathogenic characteristics of Demodex and explored the relationship between blepharitis and Demodex.The clinical diagnose methods and diagnose criteria were also summarized to improve the clinical understanding of Demodex blepharitis,which provides a useful clue for the prevention and management of this disease.

【Objective】 To investigate the in-vitro anti-inflammatory mechanism of luteolin by observing the effect of luteolin on nuclear factor-kappa B(NF?B) and COX-2 expression as well as DNA-binding activity of NF-?B in RAW264.7 cells activated by lipopolysaccharides(LPS).【Methods】RAW264.7 cells at logarithmic growth phase were allocated to blank control group,model group(treated with LPS) and luteolin groups(treated with 5,15,and 45 ?mol/L luteolin respectively).After being treated with luteolin for 30 min and then incubated with 1?g/mL LPS for 12 hours,the change of prostaglandin E2(PGE2) level was observed by enzyme immunoassay(EIA),DNA-binding activity of NF-?B was detected by electrophoretic mobility shift assay(EMSA),mRNA expression of COX-2 was examined by reverse transcription polymerase chain reaction(RT-PCR),and NF-?B and COX-2 protein expression in RAW264.7 cells was analysed by Western blotting.【Results】Luteolin obviously inhibited the formation of PGE2,decreased DNA-binding activity of NF-?B and down-regulated mRNA expression of COX-2 as well as NF-?B and COX-2 protein expression in RAW264.7 cells activated by LPS.【Conclusion】The antiinflammatory mechanism of luteolin may be related with the down-regulation of COX-2 expression by inhibiting the expression of NF-?B and DNA-binding activity.

Objective: To study the effect of Baihuzhuifeng Pills (BHZFP) on interleukin 1?(IL 1?) secreted by synoviocytes in adjuvant arthritis (AA) rats. Methods: Synovium of knee joints from AA rats were digested by collagenase and tripsin to prepare DSSC(digested synovial single cells). The content of the supernatant fluid was assayed by the radioimmunoassay. Results: BHZFP down regulated IL 1? productions of DSSC.Conclusion: It is suggested that BHZFP be probably inhibit the over proliferation of synoviocyte in AA rats by diminishing IL 1? secretion.

Objective To summarize the experience of clinical diagnosis and treatment for recurrence and progress of relieved myastbenia gravis after thymectomy. Methods 22 recurrent and progressive after relieved pa-tients with myasthenia gravis who underwent thymectomy were retrospectively analyzed. The remission therapy was conducted with combined glucocorticoid and anticholinesterase and its effectiveness was estimated. Results It was 1,17,4 as better Osserman scale Ⅰ , Ⅱ , Ⅲ respectively before operation but 15,6,1 as better Osserman scale Ⅱ , Ⅲ, Ⅳ respectively in recurrence and progress of relieved myasthenia gravis after thymectomy besides 6 with myas-thenic crisis. Complete remission and partial remission were gained in 9 patients and 12 patients respectively. There was 1 hospital-death. Conclusions Recurrence and progress can occur in any patient of relieved myasthenia gravis after thymectomy. Bulbar myasthenia gravis is usually presented as dysphagia. Reasonable administration of glucocor-tieoid could improve majority of recurrence and progress of relieved myasthenia gravis after thymectomy but responses poorly to the anticholinesterases.

Ginseng root saponins ( GRS ) was extracted from native ginseng.The dosage used was calculated according to the content of saponins.Immunosuppression in mice was induced in 15 days after implar- ting Ehrlich ascites carcinoma (EAC) subcutaneously in armpit, and in 5-11 days after implanting EAC into peritoneal cavity. GRS administered orally 50mg/kg- for 8 days did not prevent the atrophy of the thymus in tumor-bearing mice but rather aggravated it. GRS administered orally 50mg/kg for 14 days partially restored the suppressed phagocytosis of peritoneal macrophages in tumor-bearing mice. GRS administered orally 50mg/kg for 6 days somewhat restored the suppression of hemolysin formation in tumor-bearing mice 5 days after implanting EAC, But GRS given in the same route and dosage for 9 days, showed no effect on the suppression of hemolysin formation. GRS administered orally 50mg/kg for 10 days partially restored the suppression of delayed hypersensitive reaction in tumor-bearing mice.

Background Allergic conjunctivitis is common in clinical practice.But some patients,especially pediatric patients,are not diagnosed and treated correctly because of different classifications and other associated symptoms.A comprehensive understanding of the clinical characteristics of allergic conjunctivitis helps to correct diagnosis and management.Objective This study tried to analyze the clinical characteristics of allergic conjunctivitis.Methods A descriptive study was carried out.The clinical data of 745 cases of various classifications of allergic conjunctivitis who received treatment in Beijing Tongren Eye Center from April 2011 to March 2015 were retrospectively analyzed.The general status,disease history,family medical history,personal allergy history,medication history,disease course,attacked duration,affecting factors of symptoms,other non-ocular allergic diseases,other associated eye disease,the findings of the anterior ocular segment,treating outcomes were summarized and the correlation of age with symptoms was evaluated.Results In the 745 cases,75.44％ cases (562/745 were seasonal allergic conjunctivitis (SAC) and perennial allergic conjunctivitis (PAC),21.88％ cases (163/745 were vernal keratoconjunctivitis (VKC),2.42％ cases (18/745) were giant papillary conjunctivitis (GPC),and 0.27％ cases (2/745) were atopic keratoconjunctivitis (AKC).The number of patients was most in July (180,24.16％) and least in December (26,3.49％).The itching was found in 53.02％ patients,redness in 36.64％ patients,blinking in 21.88％ patients.In the patients,58.26％ patients were ≤ 14 years,34.09％ patients were 15-39 years and 7.65％ patients were ≥40 years,and a significant difference in gender was found in various age groups (x2 =89.431,P =0.000).The co-morbidities appeared to be allergic rhinitis,eczema,urticaria,allergic purpura and asthma,and the ocular coexisting conditions included dry eye,refractive error,blepharitis and meibomian gland dysfunction,corneal epithelial disorders,triehiasis and anterior uveitis.Logistic regression analysis revealed that blinking patients were more and dry eye,foreign body sensation and pain were less in ≤ 14 years patients than those in the ＞14 patiens (3=-2.039,P =0.000;3 =1.585,P=0.001;3=1.174,P=0.003;3=1.218,P =0.037).The symptoms of allergic conjunctivitis were relieved by the administration of dual-acting antiallergic agent/antihistamine drug,glucocorticoid or immunosuppressor agents.Conclusions SAC and PAC are more common classifications of allergic conjunctivitis,followed by VKC.July is a high-occurrence duration and clinical symptoms are diverse and often accompanied by other allergic diseases.The administration of dual-acting antiallergic agent/antihistamine drug,glucocorticoid or immunosuppressor agents is an effective approach to the management of allergic conjunctivitis.

[Summary] During the past 10 years, the prevalence of obesity and type 2 diabetes mellitus ( T2DM) worldwide has increased greatly.Western dietary pattern is often regarded as the primary causes of the metabolic diseases like obesity and T2DM.A number of other diets have been shown to be effective for the management of obesity and T2DM, such as the Mediterranean diet, the vegetarian diet, the low-calorie diet and the macrobiotic diet.In addition, recent studies have suggested that an imbalance of the intestinal microbiota may be involved in the development of obesity and T2DM.The main regulator of the intestinal microbiota is diet, and the composition of microbiota is impacted by long time diet pattern.This article reviews the recent advance in the influence of different diet patterns on human metabolism and gut microbiota.

Objective To explore the effects of lentivirus-mediated RNA interference targeting HOXA10 gene on the proliferation, apoptosis and drug resistance of leukemic cell line NB4.Methods NB4 cells were divided into three groups: interference group, negative control group and untreated group.The infection efficiency of lentivirus for NB4 cells was detected by flow cytometry, and the expression of HOXA10 gene of NB4 cells at mRNA and protein level was detected by real-time PCR and Western blot.Cell survival was determined by MTF assay, and apoptosis and necrosis rates were detected by flow cytometry.Western blot was used to detect the influence of down-regulation HOXA10 gene on the multi-drug resistance-1 (MDR-1) protein.Results The ratio of GFP positive cells was up to 90 ％.HOXA10 gene mRNA and protein levels were decreased in interference group compared with control group.The inhibition rate of interference group was (52.12±4.02) ％, the apoptosis rate of interference group was (30.0±2.7) ％, and their differences in the interference group and in control groups (negative control group and untreated group) were significant (P ＜ 0.05).Western blot results showed that interfering HOXA10 gene significantly reduced the resistance gene MDR-1 expression level and reverse the drug-resistant of leukemia cells.Conclusions Lentivirns-shHOXA10 can steadily reduce the expression level of HOXA10, inhibit the leukemic cells proliferation, promote apoptosis and reverse drug-resistant.HOXA10 gene is expected to become a new target for reversing leukemia drug resistance.

Objective To explore the severity of toxicity reaction after treated by the sequential chemoradio-therapy,alternate chemoradiotherapy or concurrent chemoradiotherapy in limited -stage small cell lung cancer. Methods 63 cases of limited -stage small cell lung cancer were reviewed,according to the chemoradiotherapy order,all cases were divided into:sequential chemoradiotherapy 15 cases,alternate chemoradiotherapy 25 cases, concurrent chemoradiotherapy 23 cases.The correlation of the factors(leukocypenia,gastrointestinal reaction,pneumo-nia,esophagitis)with different treatment groups after treated in 2 months,4 months,6 months were analyzed.Results Three groups of all the factors treated in 2 months had no significant change(χ2 =0.275,0.051,0.513,1.215, 0.051,0.231,all P >0.05).In 4 months the cases of sequential chemoradiotherapy >or =2 myelosuppression,the gastrointestinal reaction,the pneumonia and the esophagitis were 33.3%,33.3%,0.0%,0.0%;In the cases of alternate chemoradiotherapy >or =2 myelosuppression,the gastrointestinal reaction,the pneumonia and the esophagitis were 16.0%,4.0%,16.0%,0.0%;In the cases of concurrent chemoradiotherapy >or =2 myelosuppression,the gastroin-testinal reaction,the pneumonia and the esophagitis were 52.2%,34.8%,34.8%,4.3%;>or =2 myelosuppres-sion,each level gastrointestinal reaction and the pneumonia of the three groups treated were statistically significant (χ2 =7.054,9.702,7.947,6.145,7.373,all P <0.05).In 6 months the cases of sequential chemoradiotherapy >or =2 myelosuppression,the gastrointestinal reaction,the pneumonia and the esophagitis were 26.7%,13.3%,13.3%, 0.0%;In the cases of alternate chemoradiotherapy >or =2 myelosuppression,the gastrointestinal reaction,the pneu-monia and the esophagitis were 40.0%,56.0%,12.0%,0.0%;In the cases of concurrent chemoradiotherapy >or =2 myelosuppression,the gastrointestinal reaction,the pneumonia and the esophagitis were 69.6%,65.2%,43.5%,0.0%;each level myelosuppression,>or =2 gastrointestinal reaction and the pneumonia of the three groups treated were statistically significant(χ2 =6.174,7.663,10.544,6.286,all P <0.05).Conclusion Leukopenia and gastrointestinal reaction are closely related with chemotherapy,chemoradiotherapy results in the worsen of myelosuppression.Pneumonia and esophagitis are closely related with chemotherapy,chemoradiotherapy result in the worsen of radiation pneumonitis.