Objective To study the effect of abnormal blood pressure (BP) circadian rhythm on subtypes of acute ischemic stroke (AIS) in hypertensive patients .Methods Ninety-seven AIS pa-tients with hypertension were divided into arterothrombosis (AT)group (n=66)and small artery disease (SAD)group (n=31)according to the modified TOAST classification .Their 24 h ambula-tory BP was monitored .The 24 h BP ,daytime and nighttime BP ,morning BP surge and BP circa-dian rhythm were compared between the two groups .Results The nighttime SBP was significant-ly higher in AT group than in SAD group (133 .86 ± 18 .17 mm Hg vs 124 .42 ± 16 .06 mm Hg , P<0 .05) .The absent rate of BP circadian rhythm was higher in AT group than in SAD group (84 .8% vs 64 .5% ,P<0 .05) .However ,no significant difference was found in morning BP surge between the two groups (37 .9% vs 19 .4% ,P>0 .05) .Conclusion Absent BP circadian rhythm and elevated nighttime BP are related with AT type ischemic stroke .

Objective:To study the effect of articulation disorder on psychology action of cleft palate patients after operation.Methods:65 patients were asked to answer the psychology questionnaire.Results:The patients of misarticulation all have some degree of psychological disability,and unusual psychology action would affect pronunciation treatment.Conclusion:Patients with misarticulation have psychology problems in some degree,which would affect the clinic treatment of cleft palate.

Objective To investigate the relationship between carotid artery intima-media thickness and plasminogen activator inhibitor-1(PAI-1)in patients with metabolic syndrome(MS).Methods According to the definition of MS by NCEP-ATP Ⅲ,323 patients were divided into the MS group(160 cases)and without MS group(163 cases).The PAI-1 level was assayed by ELISA.The carotid artery intima-media thickness(IMT)and atherosclerotic plaque were measured by high-frequency Doppler ultrasound.Results The PAI-1 level,carotid IMT and incidence rate of plaque of the MS group were 30.52±11.02 ng/ml,0.92±0.21 mm and 63.1% respectively.Those of the without MS group were 26.57±11.09 ng/ml,0.86±0.20 mm and 49.1% respectively.There was a significant difference between two groups(P<0.05～0.01).Moreover,with the increasing of the number of MS components,the carotid artery intima-media thickness(IMT)and incidence rate of plaque were gradually increased(P<0.05).The stepwise regression analysis showed that PAI-1 was independently associated with the carotid IMT(standardized coefficients=0.105,P<0.05).Conclusion MS tends to cause carotid atherosclerosis,the increase of PAI-1 is possibly correlated with carotid artery atherosclerosis.

Objective: To study the effect of Baihuzhuifeng Pills (BHZFP) on interleukin 1?(IL 1?) secreted by synoviocytes in adjuvant arthritis (AA) rats. Methods: Synovium of knee joints from AA rats were digested by collagenase and tripsin to prepare DSSC(digested synovial single cells). The content of the supernatant fluid was assayed by the radioimmunoassay. Results: BHZFP down regulated IL 1? productions of DSSC.Conclusion: It is suggested that BHZFP be probably inhibit the over proliferation of synoviocyte in AA rats by diminishing IL 1? secretion.

BACKGROUND: Imported self-locking brackets cost much. So it is necessary to develop low-cost and easy-to-operate domestic self-locking brackets.OBJECTIVE: To introduce a newly developed self-locking brackets, and investigate the frictional forces of the brackets. DESIGN, TIME AND SETTING: Biomechanical controlled analysis was performed in the Laboratory of Harbin Medical University in January 2004. MATERIALS: Self-made self-locking brackets and domestic conventional tie-wing stainless steel brackets; domestic Ni-Ti round wire, Australian stainless steel round wire, and domestic stainless steel rectangular wire. METHODS: Two kinds of brackets were combined with three kinds of arch wires. Under the drive of micromotor, pull sensor moved at 1 mm/min. An arch wire, which was ligated in the bracket, was dragged. Thus, teeth's shift was imitated.MAIN OUTCOME MEASURES: Waveshape was observed through the use of storage oscillograph and corresponding data were recorded. Frictional force was determined and compared.RESULTS: Under the same other conditions, no matter which kind of arch wires were combined with, tie-wing brackets produced greater frictional force than self-locking brackets (P < 0.001). Under the same other conditions, no matter which kind of brackets were combined with, Ni-Ti wire produced greater frictional force than Australian stainless steel round wire and domestic stainless steel rectangular wire (P < 0.001). In addition, domestic stainless steel rectangular wires produced greater frictional force than Australian stainless steel round wires (P < 0.001). CONCLUSION: Self-locking bracket produces low frictional force, so it is fit for orthodontic doctors to use the corrective force.

BACKGROUND:A new open“C”-shaped molar band with nanocoating (patent number:ZL201110057699.1) has been invented to overcome some weakness of traditional bands and buccal tubes, which is used for molar fixation. OBJECTIVE:Using three-dimension finite element analysis software to theoretical y prove that the new band comes up to clinic requirement. METHODS:We established a finite element model of alveolar bone-tooth-band, and node domains tensile and shear loading were given. Stress distribution of the new bands through numerical simulation was predicted and calculated. Simulation of 20 N orthodontic force was applied in medial direction, distal direction and vertical direction in the three-dimensional model of a new band buccal tube and traditional band, to analyze the stress state in different directions under the force of the binder. RESULTS AND CONCLUSION:By numerical simulation of three-dimensional finite element, the stress of the new band was higher than that of the traditional band, but it could not reach the clinical cracking limit of resin reinforced glass ionomer cement. So the new band could theoretical y satisfy the clinical requirements.

OBJECTIVE:To investigate the dissolubility of commercial metformin hydrochloride tablets from different manufactories.METHODS:The in vitro dissolubility of 14 kinds of commercial metformin hydrochloride tablets was determined by basket method and the dissolution parameters were analyzed with variance analysis method.RESULTS:The in vitro dissolubility of 14 kinds of metformin hydrochloride tablets fitted to the request of ChP2000,but the dissolution parameters were different.CONCLUSION:The statistical results indicate that there are significant differences between products from different factories(P

Objective To compare the Poser and the McDonald diagnostic criteria for multiple sclerosis (MS).Methods Sixty-seven patients with clinical features suggestive of MS were re-evaluated by 2 neurologists utilizing both the Poser and the McDonald criteria.The Fisher' s exact test was used to statistically analyze the data from assessment by these two criteria.Results By the Poser criteria, 34 patients were classified as clinically definite MS, 24 patients as laboratory supported definite MS and 9 patients as possible MS.Under the new McDonald criteria, MS was diagnosed in 36 patients and 31 patients had possible MS.The results revealed significant differences in diagnosing MS between these 2 criteria (OR=5.549, 95% CI 2.37-13.00, P<0.01).Conclusions These 2 criteria showed remarkable differences in diagnosing MS, in particular definite MS, possibly due to greater reliance on paraclinical evidences in the Poser criteria and more stringent gnidlines of magnetic resonance imaging (MRI) in the McDonald criteria.The analysis of cerebrospinal fluid may increase the diagnostic rate of MS and specify pathologic abnormalities in MRI to some degree.

Objective To evaluate the therapeutic effect of NeuroD protein on radiation-induced intestinal injuries.Methods The expression and purification of NeuroD-enhanced green fluorescent protein (EGFP) fusion protein was performed in prokaryotic expression system.The efficiency of the fusion protein transduction into cells was monitored under fluorescence microscope.C57BL/6J mice were randomly divided into four groups with 10 mice in each group:normal control group,PBS group,EGFP group,and NeuroD-EGFP group.Besides the normal control group,the other three groups of mice received 9 Gy γ-ray total body irradiation.Intestinal tissues were collected,frozen sections were prepared to monitor the distribution of NeuroD in mice intestinal tract under fluorescence microscope,and pathological sections were prepared for H&E staining to evaluate the therapeutic effect of NeuroD protein.Results The NeuroD-EGFP fusion protein was purified by Ni-NTA column and verified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).Visible green fluorescence gathered within the cells after NeuroD-EGFP fusion protein was added in the culture medium,suggesting that NeuroD-EGFP could penetrate the cell membrane into the cells.Five hours after intraperitoneal injection of NeuroD-EGFP,visible green fluorescence gathered within the intestinal epithelial cells in villi.At 3.5 d after irradiation,NeuroD-EGFP treated mice showed significantly higher villus (F =49.49,P ＜ 0.01) and crypt depth (F =16.72,P ＜ 0.01) and more crypts per circumference (F =10.32,P ＜ 0.01) compared with PBS and EGFP groups.Conclusion NeuroD protein can accelerate the post-irradiation recovery of injured villi and crypt of intestinal tract and could be used to treat radiation-induced intestinal injuries.

Objective:To investigate the effects of different doses and infusion methods of recombinant mouse interleukin-18(rmIL-18) on the survival time and tumor diameter of the mice with hepatocellular carcinoma,and to elucidate the rational application of rmIL-18 in vivo.Methods:A total of 60 Babl/C mice were randomly divided into 5 μg rmIL-18 intraperitoneal injection group,0.5 μg rmIL-18 intraperitoneal injection group,0.5 μg rmIL-18 tumor injection group,cytotoxic T lymphocyte(CTL) intraperitoneal injection group,CTL tumor injection group and saline control group;there were 10 mice in each group.From the 10th day of inoculation,the mice in different rmIL-18 groups were injected with the corresponding doses and methods.The mice in different CTL groups were injected with tumor-specific CTL (1×106/mouse) by intraperitoneal and intratumoral injection.The mice in saline control group were injected with an equal volume (100 μL) of saline,the injections were performed 10 times.The diameters of mice were measured weekly and the survival time was recorded.Results:Compared with 5 μg rmIL-18 intraperitoneal injection group,0.5 μg rmIL-18 intraperitoneal injection group and saline control group,the tumor growth rate of the mice in 0.5 μg rmIL-18 tumor injection group was decreased (P<0.01)and the survival rate of the mice was increased (P<0.01);compared with 0.5 μg rmIL-18 intraperitoneal injection group,the tumor growth rate and the survival rate of the mice in CTL intraperitoneal injection group were decreased (P<0.01);compared with 0.5 μg rmIL-18 tumor injection group,the tumor growth rate and the survival rate of the mice in CTL tumor injection group were decreased (P<0.01).Conclusion:The best way for rmIL-18 anti-tumor effect is tumor injection and the effect has a dose-dependent manner.