Objective To establish a simple and rapid method for determination of trace chromium(Ⅵ) in drinking water. Methods In the weak acid solution containing n-butyl alchhol,trace chromium (Ⅵ) in the water connected with diphenylcarbazide and sodium dodecyl sulfate into purplish red ternary iron compound,which could be extracted by chloroform and quantified at wavelength of 550 nm. Results The detection limit was 0.001 mg/L,the RSDs were 1.1%-1.4% and the recovery rate was 96%-113%. Conclusion The method was rapid,simple and the sensitivity and accuracy will meet the requirement of standard test methods for drinking water.

[Objeetive]To report the repairing offect of cancellous bone defect with calcium sulphate.[Method]A defect 5mm in diameter and 12 mm in deep,which was drilled in the lateral aspect of rabbit lateral femoral condyle,was filled with CCaS(Citrated Calcium Sulphate)cylinder or Osteoset tablets respectively.Rabbits were killed 3,6,13 weeks after surgery.And then general oberservations and mechanical analyzing were conducted.[Result]After surgery,rabbits had good movements,spirits and eating,good healing of incision.There was no arthroedema and inflammatory response around the joints.Mechanical analyzing reports:both anticompressive strength and elasticity coefficient in the artificial bone repair bone defect area 3 weeks after surgery,were higher than in that of normal femur condyle;Anti-compressive strength and elasticity coefficient in the defect area 6 weeks,which drop comparing to 3 weeks,was lower than that of normal femur condyle;Anti-compressive strength and elasticity coefficient 13 weeks,which is higher than 6 weeks,is approaching normal.Mechanics intensity of two groups in the different time had no significant difference,but was significantly higher than one of the same time blank group.[Conclusion]To some extent,Calcium Sulphate has mechanical supportive effects on cancelloues bone defect in spite of the absorption and degradation of Calcium Sulphate.There is no significant difference between Citrated Calcium Sulphate and Osteoset tablets.

Objective To explore the effective way to decrease serious complications and to minimize the mortality following transcatheter hepatic arterial chemoembolization (TACE).Methods In 265 liver cancer patients experiencing TACE,the causes of death in 19 (20 days after TACE) were analyzed.Results 9 cases died of liver failure,4 died of the rupture of liver tumors, and 2 died of upper digestive tract bleeding.Gastroduodenal perforation,pulmonary embolism,myocardial infarction,and hyperkalemia resulted in death in one case,respectively.The mortality rates of primary liver cancer and liver metastasis were 9.2% and 1.4%,respectively (P

Based on the Clinical Medical Research Information Sharing System ( CMRISS ) , this article took thedisease-syndrome-herb study on acute exacerbation of chronic obstructive pulmonary disease (AECOPD) as a model to explore the establishment of disease-syndrome-herb study method on many types of diseases for the guidance of Chinese medicine clinical practice. The CMRISS of the First Affiliated Hospital of Henan University of Traditional Chinese Medicine, which contained 5000 format electronic medical records from August 2011 to March 2013 , was used as the database . Data preprocessing , crystal reports and complex network were used in data mining to receive the frequency relation of AECOPD disease-syndrome-herb in order to analyze medica-tion features of different Chinese medicine clinical syndromes of AECOPD . The results showed that three syn-dromes, which were phlegm-heat obstructing the lungs, phlegm-stasis obstructing the lungs, and qi-yin deficien-cy, have corresponding Chinese herbs of licorice, orange peel and earthworm. The treatment of syndrome of phlegm-heat obstructing the lungs contains herbs to clear heat, remove phlegm and activate blood circulation. The treatment of syndrome of qi-yin deficiency contains herbs to benefitqi , nourish yin as well as to activate blood circulation and remove phlegm. It was concluded that the application of CMRISS is able to process a large amount of clinical data. The data mining results can be used to guide clinical practice. It provided a better plat-form for the scientific research of Chinese medicine clinical practice .

Clinical cases of TCM are used as important clinical data to record the whole process of the interaction between doctors and patients in the form of text.However,in the context of big data,there is a lack of research on the use of information covered in clinical cases.Therefore,we studied the method of extracting the symptom term from the history of present illness in TCM clinic in this paper,in order to lay the foundation for the further use of clinical cases.First,twelve thousand,three hundred and sixty-seven history data of present illness were obtained by random selection and expert review.According to the different disease types,they were divided into the two groups of the experiments:4,838 data in the diabetes group,7,529 data in the spleen and stomach disease group and 12,367 data in the mixed or combined group.A glossary of symptom terms covering 22,996 words were compiled.Then,five feature templates,such as sliding window feature,prefix and suffix character and lexical features,were selected.CRFs model was adopted to carry out named entity extraction experiment.As a result,in the open test,the performance of diabetes,spleen and stomach disease and mixed group were (0.83,0.8,0.82),(0.9,0.9,0.89) and (0.88,0.87,0.87),respectively,while the results were (0.83,0.82,0.83),(0.95,0.95,0.95) and (0.93,0.92,0.92) in the ten-fold cross validation.In conclusion,the results showed that the CRFs algorithm was an excellent sequence labeling algorithm and applied to the named entity extraction task of symptom history.

Objective To study the sequence of learning and memory loss in the rat after hemispheric irradiation. Methods After Sprague Dawly(SD) female rats were anaesthetized with chloral hydrate, their cerebral hemispheres were irradiated with a single dose of 5,15 or 30?Gy by 4?MeV electron. On D3,D7,D30 and D60, the learning and memorizing ability was measured with the Y maze test. Results On D3 and D7, the learning ability of SD rats was impaired most but partly restored in 1 to 2 months. In observation of memory loss, the intensity of cerebral function damage was in direct proportion to the increase of radiation dose.Conclusion The learning and memorizing ability of rats can be damage by hemispheric irradiation with the severity of impairment and possibility of recruitment depending on the dose.

Objective To study the mechanism of glycerol in treatment of trigeminal neuralgia. Methods After chronic constriction injury was produced to the infraorbital nerve and the model of trigeminal neuralgia in rats was successfully established, the trigeminal ganglion and preganglionic rootlets were exposed through infratemporal fossa approach. In the experimental group, pure glycerol was instilled into the preganglionic rootlets, while in the control group physiologic saline instead. The electrophysiologic response and the results of the electron microscopy were observed at different time points after glycerol injection. Results The latency of sensory evoked potential in the experimental group was longer than that in control group. Under the electron microscope, demyelination and degeneration were very similar in two groups, but disruption of neuraxon was only observed in the experimental group. Neuraxon regeneration occurred 10 weeks later. Conclusion Retrogasserian glycerol rhizotomy in treatment of trigeminal neuralgia was through disruption of myelinated nerve fiber that is reversible.

Objective To observe the mechanism of action of panax quinquefolium diolsaponins (PQDS) on anti-ventricular remodeling in rats. Methods The model of pressure-loading ventricular remodeling was established through the method of rat’s abdominal aorta deligation. The male Wistar rats were divided into 5 groups,including sham operation group,remodeling model group,Benazepril group,and low and high dose groups of PQDS. The rats were treated with PQDS (with dose of 50 and 100 mg?kg-1 i.g) for 6 weeks. The rats in sham operation group and remodeling model group were treated with normal sodium (with dose of 10 mL?kg-1?d-1 i.g) for 6 weeks. The rats in masculine medicine group were treated by Benazepril (with dose of 10 mg?kg-1 i.g) for 6 weeks. After 6 weeks of treatment,myocardial morphological parameters,the content of malondialdehyde (MDA) and activity of superoxide dismutase (SOD) in serum,the contents of prostacycline (PGI2),thromboxane A2 (TXA2),nitric oxide (NO) and angiotensinⅡ (AngⅡ) in plasma were determined. At the same time,the contents of endothelium (ET) in plasma and myocardium were also determined. Results Compared with remodeling model group,the ventricular weight and cardiac coefficient were decreased significantly in the rats treated with PQDS (P0.05). Conclusion PQDS has protective effects on ventricular remodeling through increasing anti-oxidase activity,reducing the damage of free radicals and vasoactive substance on myocardium and correcting disequilibrium of PGI2/TXA2 in ventricular remodeling etc.

Objective To study the role of Bid in simulated ischemia/reperfusion injury in cultured neonatal rat cardiocytes and its possible mechanism.Methods A cell culture model of neonatal rat(born within 3 days) cardiocytes was used to establish simulated ischemia/reperfusion model.Unrelated siRNA or Bid specific siRNA was transfected into rat cardiocytes by Oligofectamine.The activation of Bid and caspase-8 was detected by Western blotting.The apoptotic rate of cardiocytes was determined by flow cytometry.Results Bid and caspase-8 of cardiocytes were activatied in simulated ischemia/reperfusion injury model.The activation of caspase-8 of cardiocytes in Bid-specific siRNA group decreased significantly compared with those in simulated(ischemia/reperfusion) group and unrelated siRNA group;the apoptotic rate of cardrocytes(7.4%) was lower than those in simulated ischemia/reperfusion group(51.2%) and unrelated siRNA group(48.7%)(P

Objective To develop a medical assets management system involving in early warning information platform,barcoding technology and whole-life management.Methods The system was developed based on requirement analysis as well as the technologies of Web,mobile communication,mobile application and etc.Real-time monitoring of the flows of assets and funds were realized by information flow so as to master dynamic information on medical assets.Results Real-time and dynamic management of hospital fixed and circulatory assets were executed with a department,a system and a set of reports,and the problems were solved resulting from over-centralized authority for purchase,warehousing,reporting and etc.Conclusion The system gains advantages in cost control,assets utilization efficiency and etc.