Objective To investigate and analyze nasal carriage of bacteria in medical staff and antibiotic susceptibility. Methods All study participants underwent swabbing of the anterior 1.5 cm of the nasal vestibule of both nares with a sterile swab,and did drug sensitivity test Apr.-Oct.,2007. Results In 41.08% of all samples (125/299),136 pathogenetic bacteria were isolated. Klebsiella pneumoniae,Staphylococcus aureus and Enterobacter aerogenes were the prominent bacteria,of which showed the resistance for more than three kinds of antibiotics were 35,accounting for 25.73% of all isolates,10 strains of Methicillin-resistant Staphylococcus (MRS) were detected. Conclusion The nasal carriage of bacteria and antibiotics resistance in medical staff in Tianjin should be paid attention to,for it may an important factor in the control of infection in hospitals.

OBJECTIVE To investigate and analyze hand carrier state of medical staff in Tianjin and the antibiotic resistance of the pathogens.METHODS Bacteria or pathogens were isolated from the samples using a sterilized cotton swab on the whole hand before hand washing or hand disinfection,and drug sensitivity test was performed.RESULTS In 16.72% of all samples(50/299),there were 64 kinds of bacteria or pathogens isolated.A.baumannii,Staphylococcus aureus and A.lwoffii were the most common bacteria,40.6% resistant to more then 3 kinds of drugs.Four Meticillin-resistant S.aureus(MRSA) strains were detected out.CONCLUSIONS The high rate of hand carrying bacteria and resistance in Tianjin should pay attention to.

Objective To survey the retraction of dental unit waterlines (DUWLs)in medical institutions,and evaluate the influencing factors for retraction of DUWLs.Methods In May-November 2014,dental chair units (DCUs)in medical institutions in Tianjin City were sampled through systematic random sampling method,retrac-tion volume of DUWLs was detected by independently made detector,the relevant influencing factors were investi-gated.Results A total of 58 DCUs in 30 medical institutions in 10 districts(counties)of Tianjin were investigated, the average retraction volume of DUWLs was (103.60 ±117.85 )mm3 ,the qualified rate of retraction volume of DUWLs was 48.28 %(28/58);length of utilization of DCUs was positively correlated with DUWLs retraction (r=0.52,P <0.001).There were no significant difference in retraction volume of DUWLs among medical institutions in different districts(counties),levels of medical institutions,DUC brands,and modes of DUC water supply(all P >0.05 ).Conclusion The qualified rate of retraction volume of DUWLs is low,routine maintenance of DUCs needs to be intensified,especially the long-term used DUCs,contaminatin of DUWLs due to the invalidity of retraction valve should be prevented.

Objective: To understand the natural carriage status of multidrug resistant gram-negative bacteria in ICU medical personmel and control growp of 69 hospitals in Tianjin. Methods: From April 2007 to October 2016, 2902 samples of nasal vestibule and hand swabs were collected from 1296 ICU medical personmel and 155 non medical personmel (control group) , and 14 kinds of antimicrobial agents were used to test the sensitivity of gram-negative bacilli. Results: 332 Gram-negative bacteria were isolated from medical personmel, of which 139 were multidrug resistant; 70 gram-negative bacteria were isolated from control group, and 20 of which were multidrug resistant. There was no significant difference (P>0.05) in the incidence of nasal vestibular colonization between medical personmel group (231/1296) and control group (32/155) . The control group (38/155) had a higher rate of hand bacteria carriage than medical staff group (101/1296) (P<0.01) . The proportion of multidrug resistant gram-negative bacteria was 28.57% (20/70) in control group, which was significantly lower than medical personmel group (41.87%, 139/332) (P<0.05) . Conclusion For the nasal vestibule, the medical personnel and control guoup have the same gram-negative bacteria carrying rate, but the multidrug resistant bacteria isolation rate from the medical staff group are significantly higher which may due to vocational contamination of pathogen in ICU; while the gram-negative bacteria in the hands of the medical staff group are significantly lower than those of the control group, which might attribute to the difference of hand sanitation technique of two groups.