1.Preparation of Zhitong sustained-release tablet and its release property in vitro
Haoran LU ; Xueyu ZHU ; Tiejun ZHANG ; Hongji ZHU
Chinese Traditional and Herbal Drugs 1994;0(10):-
Objective To prepare sustained-release tablet contained paeoniflorin and evaluate its drug release mechanism.Methods Orthogonal design was used to obtain the best formula.Hydroxypropykmethyl cellulose(HPMC K4M)and ethylcellulose(EC 20CPS)were used as hydrophilic matrix material,and PVP which dissolved in ethanol was used as adhesive material,then the sustained-release tablet was prepared by the wet granule compression technique.The best formula was determined by powder technology,including compression ratio,angle of repose,flow rate,and angle of contact as indexes.The optimum formula was obtained by comprehensive scoring method.The drug release in vitro was determined,and the release formula was made to investigate the release mechanism.Results The optimized prescription was with HPMC 20%,EC 20%,PVP 0%,and ethanol concentration 80%.The dissolution curves in vitro showed that the drug release could be best described by the Higuchi equation(Q=0.367 5 t1/2-0.165 8,r=0.993 2)and Peppas equation(lnQ=0.72 lnt-1.608 6,r=0.991 8).It showed that the release mechanism was Fick diffusion and backbone corrosion.The release rate could meet the requirement of quality control on sustained-release tablet in Chinese Pharmacopoeia(2005).Conclusion The method of content determination is simple and sustained-releasing rate is significant.The release mechanism follows the kinetic model of sustained-release tablet.It is worth doing further research for the clinical use.
2.Influence of negative pressure drainage on patients after proximal femoral nail antirotation fixation
Qing ZHANG ; Xiaodong LU ; Xueyu FU ; Ben WANG
International Journal of Biomedical Engineering 2021;44(1):39-43
Objective:After using proximal femoral nail antirotation (PFNA) fixation to treat femoral intertro-chanteric fractures (ITFs), closed negative pressure drainage systems are often used for drainage, but the clinical effect of this method is uncertain. A prospective randomized controlled trial study was conducted to analyze the negative effects of negative pressure drainage systems after PFNA fixation.Methods:Sixty patients with ITFs and underwent PFNA fixation were randomly divided into two groups. Patients in the drainage group were equipped with negative pressure drainage systems after PFNA fixation. At 4, 10, and 90 days after the surgery, the visual analog scale (VAS) score, analgesic dosage, thigh swelling width, wound and infection status, hemoglobin, hematocrit level, total blood loss and transfusion blood volume of the patients were recorded to evaluate the prognosis.Results:The total blood loss of the drainage group [(579.1±236.7) ml] was greater than that of the non-drainage group [(427.8±239.8) ml; P=0.01]. The transfusion blood volume of the drainage group [(443.3±176.3) ml] was greater than that of the non-drainage group [(307.8±155.4) ml; P=0.01]. Compared with the non-drainage group, the drainage group had a higher VAS score at 4 days after the surgery (2.3±0.6, P=0.02). There was no difference between the two groups in analgesic dosage, thigh swelling width, wound infection and hematoma, hospital stay and the total number of complications (all P>0.05). Conclusions:In the patients with ITFs treated with PFNA fixation, the transfusion blood volume and total blood loss of the patients in the drainage group are higher than those in the non-drainage group. In addition, drainage systems may not have short-term benefits for postoperative wound conditions.
3.Simultaneous Determination of the Contents of 9 Metal Elements in Ganmaoling Granule by ICP-MS
Xin SONG ; Xueyu HANG ; Lu WANG ; Xiaoqing FENG ; Qin WANG ; Rui XU
China Pharmacy 2015;(30):4252-4254
OBJECTIVE:To establish a method for simultaneous determination of the contents of Cu,Pb,As,Cr,Cd,Ba, Mn,Sb and Hg in Ganmaoling granule. METHODS:ICP-MS was conducted. The sample was handled by nitric acid microwave di-gestion system,the mass concnetration of 9 metal elements were determined by ICP-MS with Ge,In,Bi as internal standard and standard substance of test element standards. RF power was 1 100 W,sampling depth was 8 mm,carrier gas was argon(Ar)with high purity and flow rate was 1.0 L/min. RESULTS:All 9 elements had good linear range between mass concentration and ion peaks (r=0.999 1-0.999 9),detection limits were in the range of 0.3-6.0μg/L;RSDs of precision,stability and reproducibility tests were no more than 6.0%;average recoveries were in the range of 80.0%-113.5%,and RSDs were in 1.0%-4.5%. CONCLUSIONS:The method is simple,rapid and accurate,and can be used for the determination of 9 metal elements in Ganmaoling granule.
4.Effects of Celastrus orbiculatus Thunb.Extract on the Overexpression of mTOR in Human HepG2 Cells
Yayun QIAN ; Songhua LU ; Xueyu ZHAO ; Ting YANG ; Youyang SHI ; Feng JIN ; Yanqing LIU
World Science and Technology-Modernization of Traditional Chinese Medicine 2016;18(12):2132-2136
This study aimed at investigating the effects of the extract of Chinese herb,Nansheteng (C.orbiculatus Thunb.),on human HepG2 cells through the overexpression of mTOR.The GV238-mTOR recombinant plasmids were transfected into HepG2 cells using molecular biological technology.The expression level of mTOR was evaluated by means of relative activity of luciferase and western blot.Human hepatic carcinoma HepG2/mTOR++ cells were treated with C.orbiculatus extract in different concentrations (20,40,80,160 and 320 tg·mL-1) for 24 h.The mTOR protein expression was detected by western blot.It was found that the protein expression of mTOR in transfected HepG2 cells was significantly enhanced.C.orbiculatus extract significantly inhibited the proliferation of HepG2/mTOR++ cells.Simultaneously,C.orbiculatus extract inhibited mTOR at its protein level in a dose-dependent manner.In conclusion,we successfully constructed recombinant mTOR cloning vectors,and established the stable HepG2 cell line with the overexpression of mTOR.Besides,C.orbiculatus extract significantly inhibited mTOR protein expression in human hepatic carcinoma HepG2 cells.
5.Determination of organochlorine pesticide in aquatic products by gas chromatography
Xin SONG ; Xueyu HANG ; Qin WANG ; Lu WANG ; Xiaoqing FENG ; Rui XU ; Li MAO
International Journal of Laboratory Medicine 2016;37(6):733-735
Objective A method for quantitative determination of 16 kinds of organochlorine pesticide (OCPs) in aquatic prod-ucts by gas chromatography (GC)was established .Methods The sample was extracted by acetonitrile ,purified by Carb/NH2 ,and then determined by GC .Results The linear relations of 16 kinds of OCPs were good at 0 .05 - 1 .00 mg/L(r> 0 .998) .The limit of detection of OCPs was in the range of 0 .04 - 0 .31 μg /kg(S/N = 3) .When the standard levels were 50 ,100 ,200 μg /kg ,the recovery rates were 72 .6% - 115 .2% ,the relative standard deviations were 0 .6% - 7 .5% .Conclusion The method established in this stud-y is applied to the determination of 16 kinds of OCPs in real samples with satisfactory results .
6.Comparison among the mental profile of earthquake student, teacher and the masses survivors
Yongdong HU ; Weidong WANG ; Yanjiao LIU ; Rongjuan GUO ; Caifeng WANG ; Lan HONG ; Yingna LIN ; Fang WANG ; Menghan LU ; Xueyu LU
International Journal of Traditional Chinese Medicine 2010;32(2):166-168
Objective To investigate the prevalence and severity degree of post traumatic stress disorder(PTSD), anxiety and depression, and to explore the different mental profile in different groups involved with an earthquake. Methods 26 days after 8.0 grade earthquake in Wenchuan, psychological rescue team of State Administration of Traditional Chinese Medicine went to some communities in Deyang and Mianzhu area of Sichuan province. In this period, a total of 119 survivors, including students(42), teacher(40) and masses(37), were investigated through impact of event scale (IES), self-rating anxiety scale (SAS) and self-rating depression scale(SDS). Results①The detection rate of score over 19 in IES in 3 groups (students, teacher and masses) were 69%, 80% and 91.9%, respectively. There was significant difference among these 3 groups (F=5.611, P=0.005<0.01) in respect of severity degree of PTSD. ②The values of severity degree of anxiety in 3 groups were significant higher than normal value (P<0.001, all). There was also significant difference among these 3 groups (F=3.376, P=0.038<0.05) in respect of severity degree of anxiety, with the masses group being significant higher than student and teacher group (P=0.029, P=0.022, respectively). ③The values of severity degree of depression in these 3 groups were significant higher than normal value (P<0.05, all). But there was no significant difference (F= 0.670, P=0.514) among these 3 groups. ConclusionThe results suggested the importance to assess PTSD, anxiety and depression in post-disaster area, especially PTSD and anxiety evaluations in the masses group.
7.Determination of Carbamates Pesticide Residues in Chinese Herbal Pieces by GPC-SPE-UPLC-MS/MS
Xin SONG ; Qin WANG ; Xueyu HANG ; Lu WANG ; Rui XU ; Xiaoqing FENG
China Pharmacist 2017;20(12):2165-2168,2172
Objective:To set up an automatic gel permeation chromatography-solid phase extraction purification-ultra performance liquid chromatography-tandem mass spectrometry ( GPC-SPE-UPLC-MS/MS ) method for the quantitative determination of 14 carba-mates pesticide residues in Chinese herbal pieces. Methods:The samples were extracted by acetonitrile, and kept in constant volume with ethyl acetate-cyclohexane (1: 1, v/v). The extract was purified by GPC combined with amino solid phase extraction column (Carb/NH2). After UPLC gradient elution separation on an Agilent Poroshell 120EC-C18 column using acetonitrile-0. 1% formic acid as the mobile phase,the target pesticides were determined by tandem mass spectrometry in a multi-reaction monitoring ( MRM) mode. Results:The 14 kinds of carbamates pesticides had good linear relationships within the range of determination (r>0. 9990), and the detection limit was 0. 02-0. 35μg·kg-1 . When the spiked levels were 1, 10 and 50μg·L-1 , the recoveries were 74. 2%-108. 8%, and the RSD was 1. 25%-6. 73%(n=6). Conclusion:The method simplifies the pretreatment operation and shortens the analysis time with good selectivity and high sensitivity, which can be used for the determination of carbamates pesticide residues in Chinese herbal pieces.
8.Relation of social anxiety to self-focused attention and interpersonal needs in college students
Ying LI ; Guizhi LU ; Tonglin JIN ; Xueyu MA ; Huaibin JIANG
Chinese Mental Health Journal 2018;32(3):259-264
Objective: To explore the mediating effects of self-focused attention between social anxiety and interpersonal needs. Methods: Totally 467 college students [159 males and 221 females, mean age (21 ±2)] were assessed with the Social Anxiety Scale (SAS), Self-Focused Attention Scale (SFAS) and Interpersonal Needs Questionnaire (INQ ). The bootstrap method was used to test the mediating effect Results: The SFAS and INQ scores were higher in female students than in males (Ps <0.01). The scores of SAS, SFAS, and INQ were positively correlated each other (r = 0.20 - 0.90, Ps < 0.01). Self-focused attention has a full mediating effect between social anxiety and interpersonal needs, and the 95% confidence interval was (0.34, 0.59). Conclusion: It suggests that the effect of interpersonal needs on social anxiety may be achieved through self-focused attention in college students. Reducing the level of self-focused attention may be an important way to alleviate social anxiety among college students.
9.Correlation of cognitive function and clinical characteristics in adolescent depressive disorder patients with self-injury behavior
Xueyu JIA ; Tingting WANG ; Haibin HAN ; Jie LIU ; Lu WANG ; Bo TIAN ; Chunxia WANG
Chinese Journal of Behavioral Medicine and Brain Science 2023;32(8):707-713
Objective:To investigate the correlation between cognitive function and addiction, impulsivity, and anhedonia in adolescent depressive disorder patients with self-injury behavior.Methods:From September 2021 to October 2022, adolescents with depressive disorders who visited the outpatient department of the Qingdao Mental Health Center were enrolled and divided into self-injury group and non self-injury group based on the presence or absence of self-injury behaviors, each with 60 participants.A self-compiled general information questionnaire, the 17 items Hamilton depression rating scale (HAMD-17), the Ottawa self-injury inventory (OSI), the Chinese version of the Barratt impulsiveness scale (BIS-11), and the temporal experience of pleasure scale(TEPS) were used to evaluate both groups.The Chinese brief cognitive test(C-BCT) was used to assess cognitive function in both groups.SPSS 26.0 software was used for statistical analysis, including t-test, χ2 test, Pearson correlation analysis, and multiple linear regression analysis. Results:The self-injury group had higher scores for OSI addiction factors (9.43±8.29) and BIS-11 (67.09±11.48) compared to the non self-injury group (OSI addiction factor scores: 0, BIS-11 scores: 53.70±7.12, t=6.22, 5.91, both P<0.05). TEPS score and C-BCT scores in various dimensions were lower in the self-injury group than those in the non self-injury group ( t=-2.93, -2.01, -2.88, -2.20, -5.35, all P<0.05). Information processing speed was negatively correlated with BIS-11 score ( r=-0.296, P<0.05), and attention score were negatively correlated with OSI addiction factor score and BIS-11 score ( r=-0.303, -0.561, both P<0.05) and positively correlated with TEPS score ( r=0.364, P<0.05), including a positive correlation with the scale of anticipatory anhedonia score ( r=0.318, P<0.05). Working memory score was negatively correlated with OSI addiction factor score and BIS-11 score ( r=-0.312, -0.416, both P<0.05). Comprehensive ability and executive function scores were negatively correlated with OSI addiction factor score and BIS-11 score ( r=-0.308, -0.679, both P<0.05), and positively correlated with TEPS score ( r=0.304, P<0.05). Multiple linear regression analysis showed that BIS-11 scores were influencing factors of C-BCT dimensions ( β=-0.260, -0.592, -0.557, -1.797, t=-2.150, -3.314, -2.285, -5.165, all P<0.05). Conclusion:In adolescent depressive patients with self-injury, cognitive function is correlated with addiction, impulsivity and anhedonia, among which impulsivity is a risk factor for cognitive function.
10.Analysis of risk factors for spontaneous intestinal perforation in extremely premature infants/extremely low birth weight infants
Zhifeng HUANG ; Shan JIANG ; Panpan SUN ; Xueyu CHEN ; Xuehui ZHENG ; Yanliang YU ; Chun CHEN ; Qiuting LU ; Bingchun LIN ; Chuanzhong YANG
Chinese Journal of Neonatology 2019;34(5):358-362
Objective To analyse the risk factors associated with spontaneous intestinal perforation (SIP) in extremely premature infants/extremely low birth weight infants. Method From January 2015 to December 2018, infants with gestational age (GA)<28 weeks or birth weight (BW)<1000 g admitted to our neonatal intensive care unit were enrolled to the retrospective nested case-control study.The clinical data of SIP infants (SIP group) and infants with the same GA but without SIP (control group) were randomly selected and compared. Multivariable Logistic regression was used to analyse the risk factors of SIP. Result A total of 409 extremely premature infants/extremely low birth weight infants were born during the study period. Among them, 25 SIP infants and 55 controls were enrolled. The incidence of SIP in infants with GA 22~25 weeks was 11.8%(16/136), which is higher than infants with GA 26~27 weeks (2.0%, 5/247) (χ2=16.057, P<0.001). The incidence of SIP in infants with BW 400~749 g was 13.0%(14/108), which is higher than infants with BW 750~999 g (3.4%, 8/236) (χ2=11.343, P=0.001). Multivariate Logistic regression analysis showed that twins (OR=4.153, 95%CI 1.392~12.384, P=0.011), umbilical veins catheterization (OR=15.942, 95%CI 1.026~247.789, P=0.048) and ibuprofen use within 3 days after birth (OR=15.387, 95%CI 1.519~155.883, P=0.021) were independent risk factors of SIP. Conclusion The smaller the GA and BW, the higher the incidence of SIP. Twins,umbilical veins catheterization and ibuprofen use early after birth may be independent risk factors of SIP.