Objective To compare the effects of Jianpi Shengxue Granule and Hexue Capsule in treating patients with iron deficiency anemia. Methods All 120 patients with iron deficiency anemia were randomly recruited into group A (treated with Jianpi Shengxue Granule) and group B (treated with Hexue Capsule), with 60 patients in each group. The levels of serum iron (Fe), total iron binding capacity (T IBC), transferrin saturation (TS), transferrin (Tf), ferritin (SF), hemoglobin (Hb) and serum soluble transferrin receptor(sTIR) were observed in both groups. Results Jianpi Shengxue Granule showed better results in treating patients with iron deficiency anemia than Hexue Capsule with significant difference (P<0.05). Conclusion Jianpi Shengxue Granule is effective in treating iron deficiency anemia.

The level of the in vitro synthesis of secretory immunoglobulin A (SIgA) was determined by the sissue culture of the endoscopically biopsied gastric mucosa from patients with noncancerous gastric diseases. The amount of SIgA synthesis in chronic superficial gastritis (4.35?0.32?g/mg) and chronic atrophic gastritis (4. 82?0. 22ug/mg) was higher than that in the normal stomach (2.76?0.21 ?g/mg) (p

The aim is to investigate the effects of tumornecrosis factor ?(TNF?0 and interferon a(IFN?) on the cytotoxicity of mitomycin C(MMC) in vitro. The cytotoxicity of the three agents against gastric cancer cell line and freshly isolated gastric cancer cells were determined by the MTT assay. TNF? and/or IFN? (each 0~5000U/ml) showed cytotoxicity effects only on one of ten cell cultures dose-dependently; the combination resulted in a more significant effect. The combination of MMC with TNF? (30U/ml) or IFN?(l00U/ml) could enhance the cytotoxicity of MMC on two of three cell cultures synergisticly. When three agents were used together, the cytotoxicity of MMC against the ten cell cultures was enhanced synergisticly. TNF? and IFN? could enhance the cytotoxicity of MMC synergisticly, and this may make the cancer chemotherapy more effective.

A new group of gastric cancer associated antigens in sera of patients with rectal cancer, benign diseases and normal human were detected by MG series monoclonal antibodies against gastric cancer. The average values of normal persons plus 3 standard deviations was set as the highest limit of normal value. The positive rate were 69.7% (23/33) in sera of patients with rectal cancer, and 4%(1/25) in sera of patients with benign diseases. The values of MG-Ags were decreased to normal level 8-10 days after surgical removal in 5 patients of rectal cancer. The results of test suggest that determination of MG-Ags in serum of patients might be helpful in the diagnosis of rectal carcinoma.

Objective To explore the risk factors and targeted therapy for fungus infection in acute exacerbation of chronic obstructive pulmonary disease (COPD) pulmonary.Methods 83 acute exacerbations of COPD patients with pulmonary fungus infection were selected as the observation object(COPD fungal infection group),according to the time sequence of random month by month in acute exacerbations of COPD no secondary fungal infection of the 80 patients hospitalized patients as control group,analysis of risk factors lead to fungal infection.Results Two groups in age,live ICU time,antibiotic use time,hormone use time,albumin level mechanical ventilation people with diabetes mellitus,merger,as cor pulmonale had statistically significant differences (P ＜ 0.05) ; The conditional Logistic multiariate analysis,age,ICU patients live time,antibiotics use time,hormone use time,albumin level was acute exacerbations of COPD factors such as pulmonary fungus infection independent risk factors(P ＜0.01) ;Fungus infection were combined with antimycotic,cured 69 cases (83.13％) improved 12 cases (14.46％),there was no change in 1 case(1.20％),death 1 case (1.20％).Conclusion Patients older age,live long,long time ICU antibiotics and hormones and the low level of albumin is acute exacerbations of COPD pulmonary fungus infection independent risk factors,timely diagnosis and prognosis of patients with antifungal treatment is good.

Objective To observe the efficacy and toxicity of low dose thalidomide combined with VAD regimen contained one-week dexamethasone in treatment on patients with multiple myeloma (MM). Methods 42 patients with MM were diagnosed in our hospital.The VAD regimen contained one-week dexamethasone was vincristine 0.4 mg/d, adramycin 10 mg/d and dexamethasone 40 mg/d for 4 days. At the same time, thalidomide is given 150-200 mg/d. Results The overall response rate (ORR) is 80.95%. Before and after treatment, all the detection indicators in MM patients were statistically different (P＜0.001). There is small adverse reactions. Conclusion Low dose thalidomide combined with VAD regimen contained one-week dexamethasone in treatment of MM has more merits such as less side effects, good tolerance, convenient administration and significant curative effects, so it should be recommended in clinical works.

Objective To evaluate the effect and safety of the intermittent deferoxamine therapy on relieving iron overload caused by transfusion in senile. Method Twenty-three senile with iron overload caused by transfusion were administered in a total daily dose of 20 - 50 mg/ ( kg·d ) for 5 - 6 days per week every 4 -6 weeks,the maximal dose was 2000 mg/d, add in 0.9％ sodium chloride 500 ml, continuous intravenous drip for 6 h. Result Nine months after therapy, serum ferritin fell from (2771.5±735.3)μg/L to (2483.7 ±724.4) μg/L (P＜0.01), and urine ferritin elevated from (9.68 ±5.39)μg/L to (12.14±5.50) μ g/L (P＜0.01 ). Conclusion It shows that intermittent deferoxamine therapy can reduce the serum ferritin with no significant toxicity.

Due to lack of the practical technique to measure the biomechanical properties of the ocular cornea in vivo, clinical ophthalmologists have some difficulties in understanding the deformation mechanism of the cornea under the action of physiological intraocular pressures. Using Young's theory analysis of the corneal deformation during applanation tonometry, the relation between the elasticity moduli of the cornea and the applanated corneal area and the measured and true intraocular pressures can be obtained. A new applanation technique has been developed for measuring the biomechanical properties of the ocular cornea tissue in vivo, which can simultaneously acquire the data of the applanation area and displacement of the corneal deformation as well as the exerted applanation force on the cornea. Experimental results on a rabbit's eyeball demonstrated that the present technique could be used to measure the elasticity moduli and creep properties of the ocular cornea nondestructively in vivo.
Animals ; Biomechanical Phenomena ; Cornea ; Elastic Modulus ; Intraocular Pressure ; Muscles ; Rabbits ; Tonometry, Ocular

Combunung wuth fluorescence excutatuon-emussuon matrux ( EEM ) spectra and parallel factor analysus, suze exclusuon chromatography ( SEC ) equupped wuth multu-excutatuon/emussuon scan model fluorescence detector was used for the analysus of the composutuon of dussolved organuc matter ( DOM) usolated from landfull leachates wuth dufferent ages. The analytucal results showed that the two leachate-deruved DOMs both comprused proteun- and humuc-luke substances. However, there were four kunds of proteun-luke matter un young landfull leachates, u. e. , proteun wuth hugh molecular weught, proteun-luke matter bound to humuc-luke substances wuth hugh or low molecular weught, and peptude/amuno acuds. Whule there were only two kunds of proteun-luke matter un old landfull leachates, u. e. , proteun wuth hugh molecular weught and proteun-luke matter bound to humuc-luke substances wuth hugh molecule weught. Compared wuth SEC, EEM spectra coupled wuth parallel factor analysus could udentufy the proteun-luke matter bound wuth humuc-luke substances or those presented as non-humuc-luke substances, though ut could not udentufy the proteun-luke matter presented as proteun and that presented as peptude/ amuno acuds. The experumental results demonstrated that EEM spectra coupled wuth PARAFAC analysus and SEC could be used to characteruze proteun- and humuc-luke matter presented as dufferent specues.

Objective To investigate the serotypes of human enterovirus B ( HEV-B) species cau-sing hand, foot and mouth disease ( HFMD) and to analyze the genetic characteristics of VP1 region in cox-sackievirus B2 ( CVB2 ) and coxsackievirus B5 ( CVB5 ) strains circulating in Anyang area during 2011 to 2015. Methods Real-time RT-PCR and semi-nested RT-PCR were performed to identify coxsackievirus A16 (CVA16), enterovirus 71 (EV71) and other serotypes of enterovirus in order to obtain the complete etiologic composition of HFMD. The numbers of HEV-B serotypes and the percentages of specimens positive for every serotype in all enterovirus-positive specimens were calculated. As CVB2 and CVB5 were the pre-dominant serotypes of HEV-B species, five pairs of primers targeting the VP1 regions of CVB2 and CVB5 were designed to obtain the complete nucleotide sequences of CVB2 and CVB5 VP1 regions. The phylogenet-ic trees were constructed based on the VP1 sequences obtained in this study and those submitted to GenBank by using MEGA7. 0 and BioEdit7. 2. The selection pressures on VP1 regions of CVB2 and CVB5 strains cir-culating in China in recent years were evaluated with the online program of DataMonkey. Results A total of 57 specimens that belonged to 14 serotypes of HEV-B species were detected in Anyang area from 2011 to 2015. The 14 serotypes of HEV-B species accounted for 56% of all serotypes of enterovirus and the speci-mens positive for HEV-B species accounted for 3. 06% of all enterovirus-positive specimens. The HFMD ca-ses caused by most of the HEV-B serotypes were sporadic cases. Small outbreaks of HFMD could also be caused by some serotypes of HEV-B such as CVB2 and CVB5. The complete sequences of VP1 region were obtained from 8 CVB2 strains and 9 CVB5 strains. The phylogenetic trees based on the VP1 sequences dem-onstrated that the CVB2 strains were classified into four genotypes ( A-D) . The mean evolutionary distances between different genotypes ranged from 0. 191 to 0. 208 and the similarities in nucleotide sequences ranged from 79. 7% to 85. 8%. The CVB5 strains were classified into 6 genotypes (A-F). The mean evolutionary distances and the similarities in nucleotide sequences between different genotypes of CVB5 strains ranged from 0. 170 to 0. 285 and 76. 0% to 86. 8%, respectively. Strains of different genotypes varied significantly in the residues on positons 157 and 263 in the VP1 region of CVB2 strains and on positions 75, 90 and 95 in the VP1 region of CVB5 strains. All of the CVB2 strains isolated in Anyang area belonged to D genotype and located intensively in one lineage. The CVB5 strains circulated in Anyang area belonged to F genotype and located in two lineages. The selection pressures on CVB2 strains of D genotype and CVB5 strains of F geno-type circulating in China in recent years were 0. 037 and 0. 036, respectively. Six positively selected amino acid sites were found in the VP1 region of CVB5 strains, but no positively selected amino acid site was found in the VP1 region of CVB2 strains. Conclusion HEV-B species was an essential component of the etiologic spectrum of HFMD in Anyang area during 2011 to 2015, of which CVB5 and CVB2 were the predominant se-rotypes. The VP1 region of CVB5 was more complex and active than that of CVB2 over the course of evolution.