OBJECTIVE To investigate the distribution and resistance the bacteria isolated from the patients of our hospital,to provide reliable laboratory evidences for the clinical precaution and therapy. METHODS The antibiotic susceptibility of 17 923 bacteria strains isolated from the inpatients between 1 Jan 2005 and 31 Dec 2007 of our hospital was detected. RESULTS The top nine bacteria were Pseudomonas aeruginosa,Staphyloccocus aureus,Escherichia coli,Enterococcus faecalis,Klebsiella pneumoniae,Acinetobacter baumannii,Staphylococcus epidermidis,Stenotrophomonas maltophilia and Enterococcus faecium.S.aureus was the top one in Gram-positive bacteria(G +bacteria).The occurrence of meticillin-resistant S.aureus(MRSA) statistically decreased.The occurrence of ESBLs + E.coli statistically increased. CONCLUSIONS These bacteria are still the common detected types in the patients of our hospital.It should be noted that K.pneumoniae and ESBLs + E.coli strains are increasing gradually and they are resistant to varieties of antibiotics.

Brain metastases is a common cause of advanced tumors teeatment failure,and radiotherapy is one of the main treatment modalities.With the development of radiotherapy techniques,the survival and quality of life of patients with brain metastases have been significantly improved.Whole brain radiotherapy,stereotactic radiotherapy as well as the combination are the main treatment options,but the best fraction size,the most optional time and suitable patients need to be further investigated.Novel radiotherapy techniques such as simultaneous integrated boost have been a research hotspot.

As a neuroprotective measure,the application and research of mild hypothermia in clinical practice have been very extensive.Using mild hypothermia has achieved a positive efficacy in many diseases,such as brain damage after cardiac arrest and neonatal hypoxic-ischemic encephalopathy,etc.Although mild hypothermia has a large number of animal experiments and clinical studies in the treatment of ischemic stroke,and animal experiments have confirmed that it has the exact neuroprotective effect,the neuroprotective effect of mild hypothermia in the treatment of ischemic stroke has not yet come to a unified conclusion in the clinical studies.Its clinical efficacy does not seem to be as optimistic as expected.Therefore,it is necessary to analyze the transforming problems of mild hypothermia for treatment of ischemic stroke from animal experiments to clinical practice,and to provide ideas for the clinical application of mild hypothermia for treatment of ischemic stroke.

Objective To identify the effective traction conditions by comparing the effects of traction forces on the intervertebral disc under various mechanical conditions. Methods A three dimensional finite element model was used to carry out mechanical analysis of the traction under conditions with different forces and angles, then to carry out the comparative analysis of the stress distribution and the change of the volume of the intervertebral disc. Results In general, the stress distribution on the intervertebral disc was not even under different traction forces, The maximum stresses appeared at the back rim of the intervertebral disc. With the increase of the traction force and angle, the enlargement of the intervertebral disc volume increased, and the enlargement rate was more significant. Conclusion The volume of the intervertebral disc can be most effectively enlarged at the condition of 30?forward bending of the neck when a force of 90N was applied. This is the most effective traction condition.

Objective To explore the effect of continuous subglottic irrigation and aspiration in patients with artificial airway. Methods One hundred and twenty-eight patients with mechanical ventilation were randomly divided into observation group and control group with 64 cases in each group.The observation group was treated by continuous subglottic irrigation and aspiration,and the control group received traditional nursing.The two groups were observed and compared in terms of incidence of aspiration and ventilator-associated pneumonia.Result The incidences of ventilator associated pneumonia(VAP)and aspiration in the observation group were significantly lower than that in the control group(P<0.05).Conclusion Continuous subglottic irrigation and aspiration can effectively prevent aspiration and VAP among patients with artificial airway.

OBJECTIVE To analyze the bacteria strains isolated from the patients with acute cerebral infarction and to prevent and decrease the occurrence of hospital required infection.METHODS We studied 826 patients with acute cerebral infarction in the department of neurology,investigated the origin of infection and etiological factor,and analyzed the sensitivities to antibiotics of 1127 bacteria strains.RESULTS It was common that hospital required infection occurred in the patients of acute cerebral infarction,respiratory and urinary systems were the most common sites.Klebsiella Pneumoniae was the top one in gram-negative bacteria(18.94%),Esherichia coli was the top one in gram-negative bacteria(8.23%).CONCLUSIONS Standard medical management,effective therapy to the original diseases,proper application of antibiotics play the key roles in preventing and decreasing hospital required infection.

OBJECTIVE To explore the management methods of sterilization and supply department for the control of hospital infection. METHODS Since 2005, strict infection management system in all aspects by the sterilization and supply department has been taken including renewable medical equipment from recycle to distribution, sterile disposable medical equipment from acceptance to distribution, and the district environment and self-protection of its own staff in the sterilization and supply department and so on. RESULTS Hospital infections had not occurred as a result of sterilized supply for three years. CONCLUSIONS Since strict control and management system is carrying out, measures are effective, and good results have been achieved, which provide important guarantees for effectively controlling infection and medical safety.

OBJECTIVE To investigate the procedures and advantages of whole range quality control of disposable sterile medical appliance. METHODS By analyzing the data concerning whole range quality control of disposable sterile medical appliance from Oct 2002 to Oct 2005 in our hospital to summarize the experiences in this field. RESULTS During the last 3 years, the supply room in our hospital had administrated disposable sterile medical appliance 30 069 000 articles altogether, with the qualification ratio of 100%, and no adverse reaction was found; furthermore, the waste was reduced for no massed articles occurred; the cost accounting was raised and the error ratio during delivery was reduced too, the annual error ratio dropped form 0.003% to 0.00066%. CONCLUSIONS The whole range quality control of disposable sterile medical appliance can raise the administration quality, reduce the waste during delivery, and guarantee the disposable sterile medical appliance being used safely and effectively, also, it can provide accurate and useful data of cost accounting to concerned departments.

OBJECTIVE To analyze occupational harmful factors in department of disinfection and supply and take protective measures in pertinency.METHODS We analyzed every tache of work flow in the course of disinfection and supply,and found out the potential occupational harmful factors.Based on them,the easy and feasible protective measures were proposed.RESULTS There existed many occupational harmful factors in the course of disinfection and supply.Therefore,the working environment and occupational protection among staff should be improved.CONCLUSIONS Analysis on the peril of occupational harmful factors,enforcement of protective consciousness and establishment and implementation of operation rules and protective measures are important steps of occupational safety management for staff in department of disinfection and supply.

OBJECTIVE To analyze the cause of failure of bacteria-killing by autoclave.METHODS Under the process of using the fast-reading bio-indicator to take a routine inspect for the autoclave,while the pressure,the temperature and the time for the bacteria-killing were up to the standard,and the result of the inspection was respectively negative and positive.RESULTS For the sudden malfunction of the exhaust valve,the cold air mass was left in the experimental parcel.Therefore,the result was respectively negative and positive.CONCLUSIONS The more strict examination should be taken for the bacteria-killing tank and the advanced B-D process inquiry-device and the bacteria-killing process inquiry device in the world should be adopted to ensure that the process of bacteria-killing is qualified at all times.