Objective To explore the drugs combined with psychological therapy for lumbar disc herniation, summarize the clinical experience.Methods For 60 cases of lumbar disc herniation were treated with drug therapy, and classified as the control group, the other 60 patients in the control group on the basis of psychological intervention therapy, and classified as the observation group, two groups of patients were in our hospital from January 2016 to January 2017 were treated.Results No significant difference between the two groups of patients before treatment, the pain score, after grouping after the intervention in the observation group were obvious pain relief;compared two groups of patients with the emotional state of the visible, before treatment had no significant difference after The observation group after the intervention group were improved more significantly, shows significant difference(P<0.05).Conclusion The analysis showed that the drugs combined with psychological therapy for lumbar disc herniation, which can help patients to improve the clinical situation, adjust the patient's psychological anxiety and depression, improve the clinical effect of intervention, so it is worthy of reference.

Objective To explore the effect of family visit and nursing intervention in promoting the re-habilitation of thoracolumbar fracture patients. Methods 115 patients with thoracohmbar fracture were divid-ed into the study greup(60 cases) and the control group(55 cases) by random hospitalization number. Patients in the study group were given regularly rehabilitation guidance initiatively at home by doctors and nurses for 6 months since discharge, the control group received conventional discharge guidance. Results After the inter-vention, the two groups showed significant difference in the functional rehabilitation, awareness rate of disease-related knowledge, mastering rate of relevant skills, incidence rate of complication, satisfaction degree with nursing. Conclusions Regular family visit and nursing intervention by the doctors and nurses can not only teach patients relative functional exercise methods, promote the physical rehabilitation of limbs, reduce inci-dence of complications and solve the problem of lack of care support after discharge from hospital effectively.

Protein-coding transcriptome,pseudogenes,long non-coding RNAs and circular RNAs can act as competing endogenous RNA (ceRNA).ceRNA can crosstalk with each other through microRNA recognition elements (MRE),thus regulating the post-transcriptional gene expression.More and more evidences showed that ceRNA could form an extensive network of post-transcription regulation,keep the cell homeostasis and involve in the disease development and progression like tumorigenesis and cancer progression.Depending on up-regulation or down-regulation of different kinds of microRNA in different cells,ceRNA acts as either an oncogene or a tumor suppressor.ceRNA may have a great potential in cancer diagnosis and treatment.

Objective To investigate the effect of estrogen on expression of matrix GLA protein (MGP)in ovariectomized Sprague-Dawley(SD)rats and the role of estrogen in improving postmenopausal osteoporosis.Methods Thirty-six SD female rats were allocated into 3 groups randomly,every 12 rats in ovariectomized group(OVX group),estrogen group(E group)and control group(sham group).Rats in OVX and E group all underwent bilateral ovariectomy,those rats in E group were given by estradiol benzoate intramuscularly after 3 weeks of ovariectomy.Rats in sham group underwent bilateral lipectomy near the ovary.All rats were kept the urine and the serum every three weeks and were sacrificed after 15 weeks.The pathology changes of uterus,lumbar vertebral bones were observed by immunohistochemistry.Bone mineral density(BMD)of lumbar vertebra of rats was determined by dual energy X ray absorptiometry(DEXA).The content of MGP in serum and urine was determined by ELISA.Expression of underearboxylated matrix GLA Protein(MGP)was detected by immunohistochemistry.Relative quantification of MGP mRNA expression in lumbar vertebra bone was detected by Fluorescent real-time quantitative polymerase chain reaction.Results(1)After 15 weeks of ovariectomized,the endometrium of uterus and lumbar vertebra exhibit remarkable pathologic changes in OVX group.The serum estrogen of(454±66)pmol/L in OVX group were lower than in(527 ±77)pmol/L in sham group and(556 ±80)pmol/L in E group significantly (P ＜ 0.05).The BMD of lumbar vertebra of(0.263 ± 0.030)g/cm2 in OVX group were lower than (0.295 ±0.024)g/cm2 in sham group and(0.279 ±0.024)g/cm2 in E group significantly(P ＜0.01).(2)The serum MPG protein in OVX group and E group showed decreased trends after ovariectomized,which were(104 ±64)ng/L in OVX group and(134 ±6)ng/L in E group at 9 weeks,which reached statistical difference(P ＜ 0.05).However,MGP in urine in sham group did not exhibit significant difference after 15 weeks of surgery(P ＞0.05).The MGP in urine of E group showed increased trends after 12 weeks of surgery,which reached(110.0 ±3.4)ng/L at 15 weeks,in the mean time,it was found that(86.5 ±2.5)ng/L of MGP in urine in OVX group,which showed significant difference(P ＜ 0.05).(3)MGP could be observed in lumbar vertebra in OVX group by immunochemistry staining.In the other two groups,the expression of MGP was not dominant.(4)Relative quantification of MGP mRNA expression in lumbar vertebra was defined as 1 in OVX group,when compared with 0.289 ±0.260 in E group and 0.103 ±0.098 in sham group,the difference showed statistically significant(P ＜ 0.01).Conclusion Estrogen could increase the expression of MGP mRNA and protein in ovariectomized rats and might play an important role in improving postmenopausal osteoporosis.

Objective:To identify the HLA-DRB alleles associated with vitiligo and psoriasis in Chinese Han people of Jilin region.Methods:HLA-DRB alleles of 82 vitiligo patients and 80 psoriasis vulgaris patients were detected by polymerase chain reaction sequence-specific primer(PCR-SSP),under the comparison with 86 healthy persons.Results:①The frequence of HLA-DRB1* 07 and HLA-DRB1*12 alleles increased obviously in vitiligo group compared with control group(P

Objective:The regulatory effects of ethyl acetate extract of Herba Eclipta EAEE on immune function were studied.Methods: The experimental immunosuppressive models were induced by injection of Cyclophosphamide or hydrocortisonc.Results: The index of thymus gland spleen in immunosuppressive mice was increased significantly. when large and small dose of EAEEP were given. EAEE remarkably enhanced the delayed type hypersensitivity in immunosuppressive mice. Conclusions: EAEE possesses notable immunomodulatory action on immune system in mice.

Objective: To improve clinical nutritional support, Ten years′ experience of nutritional support in critical patients was reviewed. Methods: The 2 590 critical patients came from 19 departments in our hospital, including 20 kinds of diseases. The nutritional support ways included parenteral nutrition (PN) and enteral nutrition (EN).At the same time, some nutritional indexes were examined in patients operations for with guttural cancer and heart and in older critical patients. Results: There was a rising in enteral nutritional support for critical patients. It was obvious that the patients′ nutritional indexes had been improved after nutritional support. Conclusions: The right nutritional support is beneficial to critical patients in improving the immunity and is one of the important ways to improve the level of treatment.

Objectives:To summarize the experience of nutrition management in pancreaticorenal transplantation patients. Methods:The principle of enteral nutrition before operation is high energy,rich protein and vitamin,and increased insulin usage,in order to ameliorate malnutrition for operation.Intravenous nutrition, intravenous nutrition plus enteral nutrition, and enteral nutrition were applied respectively during the postoperative stage. Results:The body weight,circumference of upper arm and skin fold thickness of triceps muscle increased in two patients,plasma albumin and hemoglobin increased in four,and total plasma protein increased in three. Conclusions:Nutrition management of the patients is very necessary for pancreaticorenal transplantation patients,and the stepwise nutrition support after operation is feasible.Appropriate calories and nutritional elements are essential to the nutrition support,and the nutritional amelioration correlates with the condition and period of nutrition support.

ObjectiveTo observe the effect of parathyroid hormone on expression of matrix GLA protein (MGP) in ovariectomized SD rats and primary osteoblast,and to study the role of MGP on the possible mechanism of postmenopausal osteoporosis.MethodsThirty-six Sprague-Dawley female rats were allocated into 3 groups,12 in each:sham operation group,ovariectomized group( OVX group),ovariectomized and parathyroid hormone treatment group.Animals in the parathyroid hormone group were injected parathyroid hormone (20 μg/kg,three times a week for 12 weeks) three weeks after ovariectomy.All rats were sacrificed after 18 weeks.Urine and serum were collected every three weeks.Lumbar vertebral bones were observed by immunohistochemistry.Bone mineral density (BMD) of lumbar vertebra of rats was determined.The content of MGP in serum and urine was determined by enzyme-linked immunosorbent assay (ELISA).Expression of undercarboxylated Matrix GLA Protein (ucMGP) was detected by immunochistochemistry.Relative quantification of MGP mRNA expression in lumbar vertebra bone was detected by Fluorescent real-time quantitative polymerause chain reaction.Results ( 1 ) 18 weeks after ovariectomy,BMD of lumbar vertebra in OVX group was lower than those in sham group and parathyroid hormone group significantly ( P＜0.05 ).(2) The content of MGP in serum and urine was dynamic variation after treatment hy parathyroid hormone,and it was significant compared with OVX group ( P＜0.05 ).( 3 ) Immunohistochemical localization of ucMGP was seen in lumbar vertebra in OVX group.(4) Relative quantification of MGP mRNA expression in lumbar vertebra in OVX group was increased significantly compared with other groups ( P＜0.01 ).( 5 ) parathyroid hormone ( 1-34 ) in 10-7mol/L,10-8mol/L,10-9 mol/L up-regulated MGP mRNA expression in primary osteoblasts about 6.78,5.31,and 2.23 times than control respectively.It was in a dose-dependent manner.ConclusionThe effect of parathyroid hormone on the expression of matrix gla protein may play an important role in mechanism of postmenopausal osteoporosis

Objective To observe the expression of matrix gla protein(MGP) mRNA in primary osteoblasts of Sprague-Dawley (SD) rat in vitro after treatment with anti-osteoporosis agents [vitamin K2,PTH,1,25 (OH)2D3,and alendronate],and to investigate the potential role of MGP in the pathogenesis of osteoporosis.Methods Primary osteoblasts(OBs) were derived from sequential trypsin/collagenase-digested calvaria isolated from newborn SD rat (postnastal day 1-3).OBs of the second generation were identified by Van Gieson collagen staining,alkaline phosphatase(ALP) staining and calcified nodules staining.OBs of the fourth generation were selected to interfere with vitamin K2,PTH,1,25 (OH)2D3,and alendronate,then cultured for 24 h in mediums which contained various concentrations of vitamin K2 (10-7,10-6,and 10-5 mol/L),PTH (10-9,10-8,and 10-7 mol/L),1,25 (OH) 2D3(10-10,10-9,and 10-8mol/L),alendronate(10-6,10-5,and 10-4mol/L).After being cultured for 24 h,total RNA was extracted and examined by real-time quantitative RT-PCR.Results The primary cultured cells had typical morphological characters of osteoblast.van Gieson collagen staining,ALP staining,and calcified nodules staining were all positive.Vitamin K2,PTH,1,25 (OH)2D3,and alendronate could modulate the expression of MGP mRNA in osteoblasts in a dose-dependent fashion.MGP mRNA expressions were 2.56-fold,2.12-fold,and 1.57-fold with 10-5,10-6,and 10-7 mol/L of vitamin K2 treatment,respectively.The expressions were 6.78-fold,5.31-fold,and 2.23-fold with 10-7,10-8,and 10-9mol/L of PTH(1-34) treatment,8.93-fold,6.95-fold,and 3.47-fold with 10-8 10-9,and 10-10mol/L of 1,25 (OH)2D3 treatment,and 3.47-fold,2.49-fold,and 1.98-fold with 10-4,10-5,and 10-6mol/L of alendronate treatment.Conclusion Vitamin K2,PTH,1,25 (OH)2D3,and alendronate all canregulate MGP mRNA expression in calvarial osteoblasts in a dose-dependent manner.MGP seems to be a potent target of anti-osteoporosis agents,and involved in the pathogenesis of osteoporosis.