Objective To identify the effective traction conditions by comparing the effects of traction forces on the intervertebral disc under various mechanical conditions. Methods A three dimensional finite element model was used to carry out mechanical analysis of the traction under conditions with different forces and angles, then to carry out the comparative analysis of the stress distribution and the change of the volume of the intervertebral disc. Results In general, the stress distribution on the intervertebral disc was not even under different traction forces, The maximum stresses appeared at the back rim of the intervertebral disc. With the increase of the traction force and angle, the enlargement of the intervertebral disc volume increased, and the enlargement rate was more significant. Conclusion The volume of the intervertebral disc can be most effectively enlarged at the condition of 30?forward bending of the neck when a force of 90N was applied. This is the most effective traction condition.

Objective:To prepare an acellular matrix from trachea of rabbits and SD rats, and to investigate its biocompatibility.Methods: A modified detergent and enzyme link extraction procedure was performed to remove cells from the trachea of SD rats and rabbits. The histology, topography of inner-surface and biocompatibility were studied by morphological observation,cell culture and in vivo transplantation respectively.Results:The acellular trachea matrix did not inhibit the growth and amylase secretion of allogenic salivary gland cells cultured on it.The allogenically transplanted acellular trachea matrix did not result in inflammation reaction of the host tissue,could integrate with surrounding tisses.Conclusion:The acellular trachea matix is biocompatible.

s Objective: To study the feasibility of allogeneic tra ns plantation of myoblasts of SD rats for the reconstruction of facial muscle de fects. Methods: Myoblasts obtained from the forelimbs and hindlimbs of neonatal SD rats were purified and cultured. 5?10 4 myoblasts w ere seeded onto each of type Ⅰcollagen sponges in the size of 1.5 mm?0.8 mm? 0.5 cm. The myoblasts/sponge constructs were transplanted into the facial muscl e defects of syngeneic mutured rats and observed with microscopy,immunohistoche mistry and electromyography (EMG).Result:Myoblasts attac hed and kept alive on type I collagen for at lest 3～4 days in culture. 4 and 8 w eeks after transplantation,muscle like tissue with blood vessles was observed i n the implants. Expression of actin and myosin in the implants was similar to th at in the control muscle.8 weeks after implantation,the spike wave (mV) on the g rafted side and control side was 0.7?0.3 and 2.7?0.6 ( P

Objective:To study the effects of isoproterenol(Iso) on the proliferation of cultured submandibular gland cells of SD rats. Methods:Submandibular gland cells of SD rats were primarily cultured. The cells were exposed to Iso at 10 -3 g/L continuously for 8 days or intermittently(2 h each day for 8 days). The cell proliferation was sutdied by bromodeoxyuridine Brdu labeling and cell counting.Results:Iso increased cell proliferation(P

Objective The aim of this study was to investigate the correlation of serum copper levels and mental health in psychiatric shift nurses.Methods The participants were divided into day shift nurses(control group,n=60) and night shift nurses(n=60).The concentrations of trace mineral,serum biochemical levels,inflammatory and oxidative markers in blood samples of 120 shift nurses were measured.The depressive mood was measured with second edition of Chinese version of the Beck Depression Inventory,(BDI-Ⅱ).Results The night shift nurses showed moderate-to-severe depression levels,which were significantly higher than those of the day shift nurses,23.3％ (14/60) and 11.7％ (7/60) vs.6.7％ (4/60)and 1.7％ (1/60),x2=6.536,4.82,P ＜ 0.05.Night-shift nurses also had higher levels of serum copper,hs-CRP,IL-6,T-SOD and Cu-Zn SOD than the control group,(19.57±4.14) μmol/L vs.(18.03±3.20) μmol/L,1.32(0.78-1.89) rng/L vs.0.89(0.45-1.44) mg/L,68.54 (51.43-96.78) ng/L vs.56.39 (35.56-78.71) ng/L,(66.78±14.72) kU/L vs.(47.32±17.84) kU/L,(54.22±14.91) kU/L vs.(48.17±17.23) kU/L,t=-3.133～-2.057,P ＜0.01 or 0.05.BDI-Ⅱ scores correlated positively with serum copper,hs-CRP,IL-6,T-SOD and Cu-Zn SOD,r=0.248-0.457,all P ＜ 0.01.In night shift group,multivariate regression analysis showed that serum copper,hs-CRP,IL-6 and T-SOD (β=0.097-5.340,P＜ 0.01 or 0.05) were independently related factors influencing BDI-Ⅱ scores (R2 =0.609,P =0.000).Conclusions Serum copper may serve as an evaluation index for depressive mood in psychiatric shift nurses,and serum copper concentrations in night shift nurses should be monitored regularly.Clinical Trial Registry Chinese Clinical Trial Registry,ChiCTR-OOC-14005540.

Among 13 331 parturients from January 2011 to December 2013 in Peking University First Hospital, 6 162 cases required cesarean section, and the rate of cesarean section was 46.223％.The constituent ratio of cesarean section under general anesthesia was 1.75％, the constituent ratio of emergency cesarean section under general anesthesia was 60.2％, and the constituent ratio of the critically ill patients of ASA physical status ≥ Ⅲ was 16.7％.The main indication for general anesthesia was contraindication to neuraxial anesthesia or failure of neuraxial anesthesia.Of the 108 parturients who underwent cesarean section under general anesthesia, 33 cases were in laryngeal mask airway (LMA) group and 75 cases in intubation group.There was no statistically significant difference between intubation group and LMA group in terms of general data, emergency rate, percentage of patients or neonates admitted to intensive care unit, and neonatal Apgar score (P＞0.05).There were no airway-related complications such as difficult airway, aspiration or regurgitation in either group.In summary, general anesthesia was mainly suitable for cesarean section contraindicating neuraxial anesthesia and failed cesarean section, and the proportion of emergency operations was higher;LMA could be used for airway management in cesarean section under general anesthesia, but it must be placed correctly ensuring good airway sealing to prevent aspiration.

0.05). Conclusions DTI is valuable in assessing the directionality and density of white matter and can reflect the age-related physiological changes of white matter in vivo non- invasively.

One patient with congenital epidermolysis bullosa combined with pachyonychia was reported, and followed up for 11 years. The pedigree of the patient was investigated, and the loci of HLA-A, B antigens of 16 members of the pedigree were determined. The results showed that there were 60 members in the pedigree, among them 24 members had different kinds of skin diseases : 4 cases of epidermolysis bullosa combined with pachyonychia, 9 eases of albopapuloid form of epidermolysis hullosa combined with pachyonychia, 2 cases of icthyosis and 9 cases of pachyonychia simplex. The result of HLA-A, B antigens analysis of 16 members of the pedigree showed no evidence of the genes of these patients linked with HLA-A, B antigens.

From October 1969 through December 1981, 3483 esoghageal carcinomas without superficial lymphatic metastasis were treated by 60 Co in our hospital. The radiation dose was over 50Gy and follow-up was over ten years. Results: No significant difference of survival rates was found in various cancer locations in the esophagus combined with the data between our hospital and Xingtai Tumor Hospital, the 5-year survival rates for lower third esophageal cancer were 20.7%～36.4% in surgery group and only 4.3% in radiation group. The 5-year survival rates were also significantly diffrent between radiation group(34.7%) and surgery group (64.9%) for cancer length of

Objective To explore the polymorphism distribution of the hormone-sensitive lipase (HSL) gene i6 in Zunyi region. Methods HSL i6 in the Zunyi population was examined with ploymerase chain reaction-single strand conformation polymorphism (PCR-SSCP),and analyzed for its distribution. Results Six different alleles and 11 different genotypes were found in the HSL i6. The PIC is more than 0.7,in which allele 6 and allele 7 were most widely distributed.Conclusion The polymorphism of HSL i6 was identified and the result suggests in Zunyi HSL as a candidate gene of glucose and lipid metabolism.