1.EFFECTS OF 9-CIS-RETINOIC ACID ON BIOLOGICAL CHARACTERISTICS OF LUNG SQUAMOUS CANCER CELLS
Shiguo ZHANG ; Xueyan YU ; Lixi KONG
Medical Journal of Chinese People's Liberation Army 1982;0(01):-
To study effects of 9 cis retinoic acid (9 cisRA) on biological characteristics of lung squamous cancer cells, cell growth, cell differentiation and apoptotic indexes were observed in 9 cisRA treated L 78 and A 2 lung squamous cancer cells with flow cytometry. The results showed that 9 cisRA exerted marked inhibitory effect on the growth of two lung squamous cancer cell lines, 9 cisRA also had significant inducing effect on the differentiation of L 78 and A 2 cell lines, whereas the percentages of apoptosis of two cell lines in the treatment group were significantly higher than the control group. We conclude that 9 cisRA could inhibit growth inhibition, induce cell differentiation and apoptosis of L 78 and A 2 lung squamous cancer cells
2.Prognostic value of metabolic tumor volume on 18F-FDG PET/CT imaging in patients with pancreatic cancer
Peng WU ; Lijuan YU ; Xueyan LI
Chinese Journal of Nuclear Medicine and Molecular Imaging 2016;36(5):408-412
Objective To evaluate the prognostic value of MTV measured by pretreatment 18F-FDG PET/CT imaging in patients with pancreatic cancer (PC).Methods The clinical data of 52 patients (31 males,21 females,median age 58.5 years) with pathologically or clinically proved PC from January 2011 to October 2014 were retrospectively analyzed.Serum CA19-9 was measured within 1 week before PET/CT examination.SUVmax and MTV were measured respectively,and PET/CT staging was obtained simultaneously.All patients were followed up until January 2015.The CA19-9,MTV,SUVmax,PET/CT staging and clinical factors(age,gender,treatment methods) were assessed by ROC curve analysis,Kaplan-Meier analysis and multivariate Cox model.Results The median survival time of 52 patients was (11.20±7.25) months.ROC curve analysis showed that the AUC of MTV,SUVmax and CA19-9 were 0.735,0.614 and 0.527 respectively.Kaplan-Meier survival analysis manifested that the survival times were significantly different between patients with different MTV (< 12.14 cm3 vs ≥ 12.14 cm3),different SUVmax (< 8.95 vs ≥ 8.95),different PET/CT staging and different treatment methods (x2 =4.272-11.693,all P<0.05).The survival time of patients with MTV < 12.14 cm3 and that of patients with MTV ≥ 12.14 cm3 were (13.44±8.40) and (7.00± 4.82) months,respectively.Cox single-factor analyses indicated that MTV,PET/CT staging and SUVmax were risk factors of survival,the hazard ratios (HR) were 0.393,0.503,0.547 respectively (P=0.002,0.020,0.027).Cox multi-factor analyses indicated that MTV and PET/CT staging were independent risk predictors of survival.Conclusion MTV and PET/CT staging are significant factors in prognosis prediction of patients with PC,which would be helpful to make individual treatment for patients with high risks.
3.Expression of α-smooth muscle actin and collagen I in lung fibroblasts of hyperoxia-exposed newborn rats
Yu HU ; Xueyan LIU ; Jianhua FU ; Xindong XUE
Chinese Pediatric Emergency Medicine 2012;19(3):279-282
ObjectiveTo study the expression and the role of α-smooth muscle aetin (α-SMA) and collagen Ⅰ ( Col Ⅰ ) in lung fibroblasts of newborn rats with hyperoxia-induced lung injuries.Methods Thirty full-term newborn Wistar rats were randomly assigned to hyperoxia group (90% oxygen exposure,n =15 ) and air group (room air exposure,n =15) within 12 h after birth.Then lung fibroblasts were isolated and primary cultured from rat lungs on postnatal 3 d,7 d,and 14 d.The distribution of α-SMA protein were measured by immunohistochemistry.The levels of Col Ⅰ were detected by ELISA.ResultsThere were no significant differences in the levels of α-SMA and Col Ⅰ expressions between the two groups at 3 d ( P>0.05 ).While the expression of α-SMA ( 112.60 ± 4.61 vs 94.69 ± 2.38,200.30 ± 3.97 vs 103.04 ± 1.91,P<0.01 ) and Col Ⅰ protein [ ( 28.66 ± 1.15 ) μ.g/L vs ( 24.62 ± 3.15 ) μg/L,( 30.60 ± 0.65 ) μg/L vs (27.46 ± 1.68 ) μg/L,P < 0.05 ] in lung fibroblasts caused by hyperoxia were significantly higher than those in air-exposed group on postnatal 7 d and 14 d.There was positive correlation between α-SMA and Col Ⅰ protein ( r =0.72,P<0.01 ).ConclusionHyperoxia promotes differentiation of lung fibroblasts into myofibroblasts,and synthesis of type Ⅰ collegen in neonatal rats,which leads to lung fibrosis finally.
4.Dynamic changes of ERK1/2 protein in lung fibroblasts of newborn rats with hyperoxia-induced chronic lung disease
Yu HU ; Xueyan LIU ; Jianhua FU ; Xindong XUE
Chinese Journal of Emergency Medicine 2012;21(1):33-37
Objective To investigate dynamic changes of extracellular signal regulated protein kinase (ERK) 1/2 in lung fibroblast of newborn rats with chronic lung disease (CLD) caused by hyperoxia.Methods Full-term newborn rats were randomly divided into two groups:air-exposed group and hyperoxia - exposed with 90% oxygen group.Rats were sacrificed separately 3 d,7 d and 14 days after exposure to air or 90% oxygen. Then lung fibroblasts of rats were isolated and primarily cultured. By using Immunocystochemistry,Western-blot and RT-PCR methods,the levels of ERK1/2 protein and expressions of ERK1/2 mRNA were measured. Results The levels of p-ERK1/2 protein in lung fibroblast in the hyperoxia group were significant higher on the 7th day and 14th day after exposure to 90% oxygen compared with those in the air-exposed group (P <0.01 ).And the levels of total ERK1/2 protein and expressions of ERK1/2 mRNA did not change noticeably and were not significantly different between two groups (P >0.05 ).Conclusions The activation of phosphorated ERK1/2 may lead to lung fibrosis caused by hyperoxia in newborn rats.
5.Assaying of Tanshinone Ⅱ_A in Lianchuang Pills by HPLC
Weibing YU ; Xueyan BI ; Gangping ZHANG ; Zuolian ZHAO
China Pharmacy 2007;0(36):-
OBJECTIVE:To develop an HPLC method for the determination of Tanshinone ⅡA in Lianchuang pills. METHODS:The chromatographic separation was performed on Agilent Zorbax SB-C18 (250mm?4.6mm,5?m) column with column temperature at 25℃. The mobile phased consisted of methanol-water (75∶25) at a flow rate of 1.0mL?min-1.The detection wavelength was set at 270nm.RESULTS:The linear range of Tanshinone ⅡA was 0.020 2~0.202 0?g(r=0.999 9).The average recovery was 99.79%(RSD=1.01%,n=6). CONCLUSION:The method is simple, rapid and accurate, and it could be used for the quality control of Lianchuang pills.
6.Influnce of an optimized resuscitation bundle on prognosis of emergency patients with septic shock
Jiangxia XIE ; Shukun YANG ; Kaixiu HUO ; Xueyan LIU ; Dayong SUN ; Kuncheng YU
Chinese Journal of Primary Medicine and Pharmacy 2013;20(13):1999-2001
Objective To evaluate the effects of an optimized resuscitation bundle on prognosis of emergency patients with septic shock.Methods 65 patients with septic shock,admitted into emergency intensive care unit (ICU),were randomly divided into the treatment group(treated by an optimized resuscitation bundle)and the control group(treated by surviving sepsis campaign classics methods)by using random number table.The scores of sepsis-related organ failure assessment(SOFA) scores,the vasoactive agent application times,the mechanical ventilation times,the stay days of ICU,and 28-day mortality of the two groups were observed and compared.Results 3,7 day after erollment the SOFA score of the treatment group was (9.1 ± 2.5) points,(8.2 ± 2.8) points,respectively,which was lower than that of the control group[(10.1 ± 3.3) points,(9.5 ± 3.1) points] at the same time(t =4.52,3.99,all P < 0.05) ; 8-day mortality rate of the treatment group was 28.1%,which was lower than that of the control group (48.5%) (x2 =12.84,P < 0.05).Conclusion Application of an early optimized resuscitation bundle can significantly improve the prognosis of emergency patients with septic shock.
7.The changes and effects of Apelin/APJ system in LPS-induced injury of rat pulmonary microvascular endothelial cells
Huanlong LIU ; Zhongning ZHU ; Ping JIANG ; Yu HAN ; Suwen SU ; Xueyan CHEN
Chinese Pharmacological Bulletin 2015;(8):1152-1158
Aim To explore the changes of Apelin/APJ system in LPS-induced injury of rat pulmonary mi-crovascular endothelial cells( PMVECs) , and the effect and mechanism of Apelin. Methods PMVECs were cultured with the explant technique, and the identifica-tion of rat PMVECs was carried out by immunocyto-chemical staining of factorⅧrelated antigen. MTT as-say was used to evaluate the viability of PMVECs. The mRNA expression of Apelin and APJ was detected by RT-PCR. The protein expression of PCNA and the phosphorylation of Akt was analyzed by Western blot. Results The mRNA expression of Apelin and APJ showed a compensatory increase after LPS treatment for a short period of time ( P<0. 01 ) , but with the exten-sion of time, which was significantly inhibited, even lower than the control group ( P<0. 05 or P<0. 01 ) , suggesting that Apelin/ APJ system might be involved in LPS-induced PMVECs injury. MTT results showed that 10 -6 ~10 -9 mol · L-1 Apelin obviously promoted the proliferation of rat PMVECs ( P <0. 05 or P <0. 01 ) , and with certain concentration and time de-pendence. Moreover, Apelin also improved the LPS-induced PMVECs injury in different degrees ( P<0. 05 or P < 0. 01 ) . In addition, Western blot analysis showed that Apelin significantly reversed the decrease of the protein expression of PCNA and the Akt phos-phorylation level induced by LPS ( P <0. 05 or P <0. 01 ) . Conclusions The Apelin/APJ system is in-volved in LPS-induced PMVECs injury. Apelin plays an important role in protecting the pulmonary microvas-cular endothelial function and reversing the LPS-in-duced PMVECs injury, which might be related to the activation of Akt phosphorylation pathway.
8.Antisense oligonecleotides of CT120 inhibit the growth of lung adenocarcinoma cell line A549
Zunling LI ; Shuhong SHAO ; Shuyang XIE ; Fei JIAO ; Shouseng SHI ; Xueyan YU
Basic & Clinical Medicine 2006;0(04):-
Objective To clarify the relationship between CT120,a novel human plasma membrane-associated gene,and the proliferation of lung adenocarcinoma cell line A549.Methods Expression vector(pcDNA3.1)containing antisense oligonucleotides of CT120 was constructed and transfected into the adenocarcinoma cell line A549.RT-PCR and Western blot detected the expression of CT120.Meantime,flow cytometry and soft agarose colony formation were applied for cell proliferation,and P53,CyclinD1 and CDK4 were detected by RT-PCR.ResultspcDNA3.1 containing antisense oligonucleotides of CT120 was successfully constructed and inhibited the expression of CT120 effectively by RT-PCR and Western blot.The expression of P53 was up-regulated and the expression of CyclinD1 and CDK4 were down-regulated.Conclusion The down-regulation of CT120 expression by antisense oligonucleotides technique may be a potential drug target for treatment of lung cancer.
9.Effects of 9- cis- retinoic acid on transcription of cyclin D1, cdk4 in lung adenocarcinoma cells
Guijie REN ; Zhifang LIU ; Lei DING ; Guoqiang HU ; Xiaoyan HU ; Keli TIAN ; Xueyan YU
Chinese Journal of Pathophysiology 1986;0(03):-
AIM:To investigate the mechanism of 9-cis-RA inhibiting the growth of lung adenocarcinoma cells, and we detect the expressional changes of cyclinD1 and cdk4 in lung adenocarcinoma cells PG, A_(549), SPC-A_1 before and after being treated with 9-cis-retinoic acid(9-cis-RA). METHODS: RT-PCR was used to analyse the transcriptional changes of cyclinD1 and cdk4 in PG, A_(549) and SPC-A_1. RESULTS:9-cis-RA decreased the transcription of cyclinD1 in PG and A_(549)(P