Objective To prepare and purify the polysaccharides in Shuanqian fungal substance (FS);To study its monosaccharide composition and property. Methods Strychnos nux-vomica was under a thirty-day solid fermentation through Trametes cinnabarina. Then crude polysaccharide was got after the process of drying, smashing, water boiling, condensing, decoloring, removing protein and ethanol precipitation. The content of polysaccharides in Shuanqian FS was determined by phenol-sulfuric acid method. Dialysis and gel chromatography method was used to purify the crude polysaccharide. The purified composition was analyzed by acid hydrolysis and thin layer chromatography. Results The content of crude polysaccharide was 47.68%and yield was 10.52%. It showed that polysaccharide in Shuanqian FS contained glucose and galactose, but no nucleic acid and protein. Conclusion Polysaccharide in Shuanqian FS was an offwhite powdery heteropolysaccharide, which contained glucose and galactose.

Objective:To optimize the extraction procedure of total alkaloid in Radix Aconiti.Methods:The orthogonal design(L9(34) ) was carried out for the optimum extraction conditions guided by yield of the extract and the content of aconitine,hypaconitine and mesaconitine determined with RP-HPLC method,the mobile phase was MeOH-H2O2-CHCl3-DEA(70∶30∶ 2∶0.1).Results:The best extraction process was as follows:moisten with ammonia test solution,then leach for 24 hours with 15 times of ether.Conclusion:The optimum extraction methods are rational.

ABSTRACT:Objective To investigate the clinical value and application indications of the second biopsy-cervical conization in the diagnosis and treatment of cervical lesions.Methods We selected 413 patients from Department of Gynecology and Obstetrics,General Hospital of Jinan Military Command,who received cervical multi-point biopsy pathology IA for cervical cancer and cervical conization (LEEP knife)of the second biopsy between January 2012 and October 2015.Their data were retrospectively analyzed.Results Compared with cervical multi-point biopsy, the second biopsy-cervical conization after operation had pathological upgrade in 10.65% (44/413),agreement in 73.37% (303/413),and reverse (pathological levels drop and overcast)in 15.98%(66/413).Cervical multi-point biopsy and the second biopsy-cervical conization significantly differed in the diagnosis of cervical low-level intraepithelial lesion,high-grade intraepithelial lesions,and early invasive cancer (IA)(T=21.740,v=3-1=2,P<0.05).Before conization high-risk type HPV infection positive rate was 71.91%(297/413);after operation it was 86.36% (38/44)in upgrade pathology and 70.20% (259/369)in non-upgrade one.And thin prep cytologic test (TCT)showed that the positive rate (ASC-US and above)was about 87.89% (363/413), of which about 11.85% (43/363)was pathological upgrade after conization.The positive rates of high-risk type HPV infection and TCT result (ASC-US and above)in cervical lesions differed significantly between pathological upgrade and non-upgrade after conization (χ2=5.092,P<0.05,χ2=4.476,P<0.05).Conclusion The second biopsy-cervical conization technique as a means of reevaluation of cervical pathological changes in diagnosis and treatment of cervical biopsy under colposcopy can significantly improve diagnosis rate,reduce misdiagnosis and occult cervical cancer,but its clinical application has some indications.

Objective To optimize the extraction technology of alginic acid in Laminaria japonica Aresch. Methods The influence of the amount of solvent, the extraction time and temperature (3 factors) on the alginic acid content and yield was investigated by orthogonal test L9(34), and carried on to inspect the methodology. Result The optimum extraction condition was A1B1C2, that was adding 30 times solvent (1%Na2CO3), whisk extracting for one hour at 60 ℃. Conclusion The temperature is the most important factor. The content of alginic acid decrease obviously at above 60 ℃. The optimum extraction technology is reasonable.

To evaluate the clinical therapeutic effect of limited bronchus granuloma caused by foreign body with medication in trachea, 6F tube was put in sick trachea under fibrobronchoscopy in 26 patients with bronchus granuloma due to foreign body, and 5ml of 2% lidocaine + 10ml of sterile Saline + 5mg dexamethasone +antibiotics (Amikacin0 2g or ceftriazone1 0g) were injected into sick trachea through 6F tube twice a day. 25 were cured, the cure rate was 96 2%,and the other one had to be operated; the course was 6～15d. Other 29 patients were treated with routine antibiotics intravenation+aerosol inhalation or medication in trachea under fibrobronchoscopy every other day, and 24 were cured, the cure rate was 82 8%; the other 5 had to be operated, the course was 6～32d. It is suggested that the former method has good effect in local anti inflammation, and can decrease the intravenous antibiotics dosage and side effect.

Objective To observe the curative effect of simvastatin to treat carotid atheroma. Methods 106 patients with carotid atheroma were randomly divided into two groups. The treatment group( n=56) was given orally 20 micro-gram of simvastatin every night, while the matched control group( n = 50) was given 100 microgram of aspirin enteric-coated everyday for 4 months. Results After four months of follow-up, the carotid artery inner-intermediate thickness and the speckle's size,examined by the color Doppler, were obviously decreased ( P 0.05). Conclusion Simvastatin can obviously ameliorate the carotid atheroma. It can be served as one of the normal selections for treating the carotid atheroma.

OBJECTIVE To investigate the difference of IgM and DNA for Mycoplasma pneumoniae in respiratory infected children with different age.METHODS Serum samples were tested with ELISA for IgM and molecular beacon based real-time PCR was used to test M.pneumoniae DNA.RESULTS M.pneumoniae DNA was detected in 40% of the patients and the IgM was positive in 11% at the day of sampling and 30% after 7 days,respectively.The difference was of significance(P

Objective To evaluate the clinical applied value of multiplanar reconstruction(MPR) and three dimensional reconstruction(3D) with spiral CT in the fractures of the elbow joint.Methods Multi-slice helical CT scanning was performed in 24 cases with elbow joint injure,MPR and 3D image reconstruction of elbow joints were carried out.Results 42 fractures and 4 dislocations of elbow joints in 22 cases were found on the images of MPR and 3D,29 fractures were displayed on 2D CT,39 fractures were found on MPR and 35 fractures were viewed on 3D.Conclusion MPR is better than 3D in finding the small fractures and in viewing damage inside the bone of elbow joint,however 3D is better than MPR in showing the location,range,shift of fractures.MPR and 3D with spiral CT have important values in diagnosis of elbow joint fractures.

Objective To study the effectiveness of comprehensive control measures based on systematic ecological system construction to interrupt the transmission of schistosomiasis in hilly endemic regions in Sichuan Province ,so as to provide the ev?idence for adjustment of schistosomiasis prevention and control strategies. Methods A high endemic area of schistosomiasis, Panao Township of Dongpo District in Meishan City,was selected as a demonstration area. The comprehensive measures for schistosomiasis control with focus on systematic ecological management were implemented,and the income of residents,index?es of schistosomiasis control effect and so on were investigated before and after the intervention and the results were compared. Results The project based on systematic ecological system construction started in 2009 and 317.351 million Yuan was put into the construction. The construction included economic forest plant base(1 866.68 hm2,72.66%of the total farmland areas),eco?logical protection gardens(585.35 hm2)and so on. Totally 97.04% of historical areas with Oncomelania hupensis snails were comprehensively improved. In 2015,the peasants' pure income per capita increased 4 938 Yuan,with the average annual growth rate of 14.69%. All the farm cattle were replaced by the machine. The benefit rate of water improvement was increased by 52.84%and the coverage rate of harmless toilets increased by 18.30%. The positive rate of serological tests for schistosomiasis decreased from 7.69%to 3.50%,and the positive rate of parasitological tests decreased from 1.18%to 0. The area with snails was decreased from 23.33 hm2 to 0. The awareness rate of schistosomiasis control knowledge and correct behavior rate of the resi? dents increased from 85.50%and 82.60%to 95.70%and 93.90%respectively. Conclusions The comprehensive schistosomia?sis control measures based on systematic ecological management are conform to the currently actual schistosomiasis prevention and control work in hilly endemic regions,and have good ecological economic benefit and schistosomiasis control effectiveness, which provide an effectively new model of prevention and control for advancing process,consolidating the effect,finally realiz?ing goal of interruption and elimination of schistosomiasis in hilly endemic regions.

Objective To access evaluate the efficacy and safety of on-line hemodiafiltration for treating a-cute cerebrovascular disease (ACVD) patients complicated with hyperosmolar nonketotie diabetic coma (HNDC).Method Totally 11 patients of ACVD complicated with HNDC were observed for this prospective control study.All of them underwent on-line hemodiafiltrafion for 90 minutes using Fressnius 4008S Hemodialysis Machine withONLILNEplus TM and F60 one hour after final diagnosis was made. The bicarbonate ultrafiltrate rate was set at 500ml/min and blood flow at 150 ～ 180 ml/min; and the substitute fluid were infused with post-dilution at 50 ～ 60ml/min. The symptom of brain edema and cardiac insufficiency during the same course of treatment was observed.Blood were taken from the patients to detect serum kalium, serum glucose, serum natrium, BUN and plasma os-motic pressure at one hour before treatment and six hours after treatment, respectively. The change of in conscious-ness and adverse effects were evaluated at 24 hours after treatment. Results All patients were treated successful-ly. The blood glucose, serum natrium, serum kalium, BUN and plasma osmotic pressure were decreased after on-line hemodiafiltration. Among the 11 patients, consciousness was improved obviously in 8 patients, 3 patientsdied, accounting for 73% of successful effectiveness. Conclusions The on-line hemodiafiltration was effectiveand safe for treating ACVD patients complicated with HNDC.