Chorionic villi of 6-9 week normal pregnant women were obtained twenty-four hours after exposure to diagnostic ultrasound and observed under transmission electron microscope. Trophoblastic syncytium and trophoblastic cell showed remarkable ultrastructural changes which included:① cellular membrane lost its integrity, ② cytoplasm escaped into the interstitial arca, ③ endoplasmic reticulum dilated and ④ mitochondria swelled, cirstane reduced with small vesticle and irregular morphology.

Acetylation of lysine residues of core histone N-terminal domains has been found correlatively associated with transcriptional activation in eukaryotes. Recent studies showed that some transcriptional regulators with histone acetyltransferase(HAT) and deacetylase(HDAC) activities may play a causative role in regulating gene expression. The link between tumorigenic processes and misregulated or mistargeted HAT and HDAC activities has been reported.

Objective To investigate the effect of two kinds of permeation enhancers,azone and clove bud oil,on transdermal permeation of Shuxiong cataplasm. Methods Cataplasm containing azone and clove bud oil,at different concentrations were prepared,with ferulic acid as the index,the role of enhancing permeation of azone and clove bud oil for the main active component were studied by in vitro permeation experiments. Results Both azone and clove bud oil at different concentrations could increase permeation. Clove bud oil was better than azone and the best concentration of Clove bud oil is 3%. Meanwhile,the permeation enhancing multiple of clove bud oil group was the highest which was 3.68. Conclusion With the cumulative permeation quantity of ferulic acid as the index,3% clove bud oil is more suitable to act as a permeation enhancer of Shuxiong cataplasm.

We carried out a dietary investigation of pupils aged 10 - 11 in Mei county in Shaanxi with level-based community random sample method. The result showedthat the average intake of daily food was mainly grain. That of energy was less than 70% of RDA and 60% of WHO. That of the protein was less than 70% of RDA in male and 60% of RDA in female. That of the other nutrients was less than 60% of RDA except iron, nicotinic acid and thiamine. Energy allocation among three mels was not adequate in male pupils. There waas higher energy in breakfast and supper, not sufficent at lunch.

Objective To investigate the association of the polymorphism of serotonin transporter gene linked polymorphic(5-HTTLPR) region with depression and its clinical symptoms in the Han Chinese.Methods PCR-based technique was used to genotype 5-HTTLPR in 64 patients with major depression and 67 unrelated controls,and the association between the polymorphism and clinical symptoms of depression was explored according to HAMD score.Results Fewer patients tended to be of L/L genotype(9.4%) than controls(19.4%),but there was no significant difference.Among women,fewer patients tended to be of L/L genotype(2.9%) than controls(13.9%),but there was no significant difference.The symptom analysis showed that depressive symptoms were significantly associated with 5-HTTLPR genotypes or alleles in the patients of L/L genotype,and that L allele had significantly lower depressive scores compared with those of other genotypes or alleles(P

Objective To examine the cellular distribution and expression,activation of nuclear factor ?B(NF-?B) in the tissuses of chorion of early spontaneous abortion,and explore the relationship between early spontaneous abortion and NF-?B.Methods The NF-?B expression on the chorionic villi was analyzed by S-P immunohistochemistry.The nucleoplasmic ratio of NF-?B was detected by laser scanning confocal microscope.Results The expression intensity of NF-?B in the syncytiotrophoblasts was significantly weaker in women with early spontaneous abortion than in the normal early pregnancy(P

Objective To investigate the effectiveness of Xingding injection on glomeruloscerosis in rats treated with adriblastine.Methods Twenty-four rats were randomly divided into three groups.The rats in three groups were injected with normal saline(NS),NS associated with adriblastine,adriblastine associated with Xingding injection respectively.The content of protein in urine of 24 hours and total cholesterol(Chol),total protein(TP),albumin(Alb),creatinine(Cr) in serum were detected.Results In Xingding group,the contents of protein in urine of 24 hours and Cr in serum were significantly lower,and that of serum Alb was significantly higher compared with those of the control group(P

Objective To explore methylprednisolone and conventional dose prednisone treatment for hematological damage in systemic lupus erythematosus(SLE) in the near future response. Methods Hemocytopenia in 147 patients with SLE were treated by intravenous injecting methylprednisolone and conventional dose prednisone and therapy response were observed in the tenth day after treatment. Results The responses were obtained in methylprednisolone and in conventional dose prednisone increased percentage of Hb were 34.8% and 14.0%,of WBC were 76.7% and 63.0%,of Pt were 66.7% and 27.3% in two group respectively. In comparison of values of Hb,WBC,and Pt before treatment with those after treatment showed significant difference in two groups(P

Background Choroidal neovascularization (CNV) leads to blindness in many fundus diseases.Study showed that naringenin suppresses CNV,but it presents with poor bioavailability because of its poor solubility in water.β-cyclodextrin (β-CD) can increase the water-solubility of drugs, however, whether the inhibitory effect of naringenin on CNV can be improved after clathrated with β-CD remains unclear.Objective This study was to compare the inhibitory effects of naringenin with naringenin/β-CD compounds on CNV in rats.Methods Naringenin/β-CD clathrate compounds were prepared with saturated solution,the solubility of naringenin in water was calculated based on standard curve.Thirty-two male Brown Norway rats were randomized into normal control group, model control group, naringenin group and naringenin/β-CD group.Laser-induced CNV models were created in the right eyes of rats from the model control group, naringenin group and naringenin/β-CD group.Naringenin and naringenin/β-CD clathrate compounds were intraperitoneally injected at a dose of 20 mg/kg in the rats of naringenin group and naringenin/β-CD group since the day after modeling, respectively, once per day for 4 weeks, and equal volume of DMSO was injected in the same way in the model control group.Fluorescein isothiocyanate dextran (FITC-D) was injected via rat hypoglossal vein for the preparation of flatmounts of choroid in the fourth week,and the areas of CNV were measured and compared among the groups.The retinal pigment epithelium (RPE)-choroid-sclera tissues were isolated from the rats, and the relative expression levels of vascular endothelial growth factor (VEGF) , cyclooxygenase-2 (COX-2),phosphatidylinositol-3-kinase (PI3K),p38mitogen-activated protein kinase (p38MAPK), matrix metalloproteinase (MMP)-2, MMP-9 mRNA and their proteins in RPE-choroid-sclera tissue were detected using real-time PCR and Western blot.Results The solubility of naringenin in water increased by 11.8 folds after encapsulated with β-CD.The CNV areas in the model control group, naringenin group and naringenin/β-CD group were (34.56± 1.67), (20.90± 1.47) and (13.20± 1.38) × 103 μm2 , respectively, showing significant reduces in the naringenin group and naringenin/β-CD group compared with the model control group (t =3.973 ,P＜O.05;t =5.532, P＜0.01) ,and the CNV area in the naringenin/β-CD group was significantly smaller than that in the naringenin group (t =3.605,P＜0.05).The relative expression levels of VEGF, COX-2, PI3K, p38MAPK, MMP-2, MMP-9 mRNA and their proteins were significantly declined in the normal control group,naringenin group and naringenin/β-CD group in comparison with the model control group (all at P＜0.05).In addition, the expression levels of VEGF mRNA and COX-2 mRNA and their proteins were significantly lower in the naringenin/β-CD group than those in the naringenin group (all at P＜0.05).Conclusions The naringenin/β-CD clathrate compounds can improve the water solubility of naringenin and enhance their inhibitory effect on rats CNV.The inhibitory effect of naringenin on rats CNV probably is associated with anti-inflammatory pathway.

Based on the competition reaction of target protein, aptamer probe, padlock probe and complementary sequence, a highly sensitive fluorescent aptasensor was developed in this study in combination with rolling circle amplification. In the absence of target protein, the ligation-rolling circle amplification reaction was repressed because the complementary sequence hybridized with aptamer probe to form double-stranded duplex. While in the presence of target protein, the target molecules bound specifically with aptamer probe, inducing displacement of the complementary sequence and hybridization with padlock probe. The padlock probe was circularized with the assistance of E. coli DNA ligase, and the rolling circle amplification process could be accomplished by Phi 29 DNA polymerase. The amplification product contained thousands of repeated sequences which could hybridize with the loop of molecular beacon ( the detection probes) , resulting in a significant fluorescence signal. The effects of length of complementary DNA ( CDNA ) sequence and concentration of padlock probe were investigated. Under the optimized experimental conditions, the model target protein thrombin could be highly sensitively detected by the proposed aptasensing system in a linear range of 0 . 067-32 . 4 nmol/L with a detection limit of 0 . 03 nmol/L ( approximately 90 amol target molecules). Moreover, the presented sensing method was universal for other target analysis by skillfully design of the sequence of aptamer probe and related oligonucleotides.