Objective To investigate the status of high-risk human papilloma virus(HR-HPV)infection and natural clearance in males whose wife have been infected,and to analysis the coincidence rates of HPV subtypes in couples.Methods Urethra epithelial cells and urethral secretions of 246 males whose wife infected with HR-HPV have been enrolled in the study to detect 13 kinds of HPV subtypes and redetect after 1 year.Results The natural clearance rate of HR-HPV in male was 31.82％(35/110).The infection rate was 44.72％(110/246)in initial detection,while 30.49％(75/246) in redetection,the difference was statistically significant(χ2=10.612,P<0.05).The coincidence rate of HPV subtypes in couples was 15.04％(37/246)in initial detection,while 8.94％(22/246)in redetection,the difference was statistically significant(χ2=4.333,P<0.05).The frequency percentage of HPV16,18 increased significantly in male,the difference was statistically significant(χ2=35.991,P<0.05).Conclusion HR-HPV can be cross-transmitted between infected women and their spouses.It is necessary to detect for her husband once women have been detected high-risk HPV positive.

Objective To measure the scope of normal meniscus by magnetic resonance imaging(MRI),and create a database of meniscus size of each part.Methods The MRI image measured rake angle and back angle,body height and width of each part and the angular distance of rake and back angle of 303 meniscus of normal knee joint.Results The width and height of anterior horn,anterior exterior angle,body,angulus posteriolateralis and back angle of the medial meniscus were (5.67 ± 1.37) mm and (3.53 ± 0.86) mm,(7.24 ± 1.91) mm and (4.38 ±1.01) mm,(5.61 ± 1.73)mm and (4.25 ±1.07) mm,(11.01 ±3.53) mm and (5.41 ± 1.66)mm,(9.21 ±2.71)mm and (5.02 ± 1.39)mm,respectively.Rake and back angle of angular distance was (38.51 ± 10.88)mm.The width and height of the anterior horn,anterior exterior angle,body,angulus posteriolateralis and back angle of the lateral meniscus were (6.95 ± 1.69) mm and (3.83 ± 0.91) mm,(7.48±1.90)mmand (4.06 ± 0.97) mm,(6.51± 1.79) mm and (5.10±1.01)mm,(7.62±1.99)mm and (5.18±1.19)mm,(6.68 ± 1.41) mm and (5.64 ± 1.12) mm,respectively.The rake and back angle of angular distance was (38.51 ± 10.88) mm.Conclusion In this study,we obtained the data of meniscus for normal people by MRI,which provides reference values for diagnosis of meniscus injury and transplantation.

To investigate the effect of calcium concentration in dialysate on the QT interval (QT) and QT interval dispersion (QTd) during hemodialysis(HD) in patients with end stage renal disease (ESRD), Ca 2+ concentrations of 1 25mmol/L (dCa 2+ 1 25), 1 5mmol/L (dCa 2+ 1 5) and 1 75mmol/L (dCa 2+ 1 75) in dialysate were given to 15 ESRD patients for five times. The QT and QTd were measured before and after the five HD sessions. The results showed: With the dCa 2+ 1 25 HD, serum Ca 2+ and plasma calcium were decreased (1 22?0 08 vs 1 17?0 07 mmol/L and 2 43?0 34 vs 2 28?0 16mmol/L, P 0 05). The changes in the QT correlated inversely with the changes in serum Ca 2+ and plasma calcium ( P

To study the treatment of peritoneal and hepatic recurrence of advanced gastric carcinoma after operation, 218 patients were divided into treatment group and control group.Treatment group: (hyperthemic peritoneal perfusion and intraarterial infusion chemotherapy)126 cases. Control group (peripleral veins): 92 cases. The peritoneal and hepatic recurrence and 3 year survival rate of each group were analyzed. The peritoneal and hepatic recurrence and 3 year survival rate in treatment group were 27%, 12 7% and 69 5%. and were 44 5%, 26 1% and 47 8% in control group. Hyperthermic peritoneal perfusion and intraarterial infusion chemotherapy are effective in control of the recurrence of advanced gastric cancer after operatin.

Objective To explore the causes,prevention and treatment of postoperative respiratory failure in patients with lung cancer.Methods The clinical data of 659 patients,admitted in Changzheng Hospital from Jan.2000 to Dec.2006,with lung cancer and suffered from postoperative respiratory failure were analyzed retrospectively.Preoperative lung function,past medical history,the etiology and preventive procedures,and the treatment methods for postoperative respiratory failure were also reviewed.Results Postoperative respiratory failure was occurred in 42 of 659 cases,the incidence was 6.4%,and all the 42 cases occurred in 1 to 5 days after operation.The major etiopathogenisis of postoperative respiratory failure included respiratory infection,bronchial asthma,operative wound,postoperative incision pain,preoperative cardiopulmonary dysfunction,etc.The respiratory failure was controlled in 37 of the 42 cases by mechanical ventilation and symptomatic treatment.Two cases died of multiple organ failure,another 2 cases died of respiratory distress syndrome and 1 case died of severe respiratory tract infection,the mortality was 11.9%.The preoperative lung function indexes of patients with postoperative respiratory failure were significantly lower than that of those patients without postoperative respiratory failure(P

Objective: To investigate the feasibility of tracheal prosthesis made from biomaterials in the replacement of the circumferential tracheal defects. Methods: Surgical resection and replacement of the cervical trachea with different prosthesis was performed in 16 mongrel dogs. Bronchoscopic, roentgenographic, macroscopic and histopathologic examinations were employed to evaluate the efficacy of the tracheal substitutes. Results: In group A (with type-I prosthesis, n=8), one dog died from prosthetic dehiscence on 96th days postimplantation, another one died from anastomotic leakage caused by severe infection in cervical incision on postoperative 45th days. The other 6 of group A had uneventful postoperative courses and did not show any respiratory symptoms. Tracheal prostheses were completely healed with the recipient's trachea and combined in its integrity. Varied degrees of epithelial lining were observed to have formed near both the anastomotic ends. In addition, many scattered and varied sizes of neo-ossification substances were found at the sites surrounding implanted prostheses. In group B ( type-II prosthesis ,n=4), dehiscence and dislocation of implanted prostheses often occurred with time after operation, which inevitably resulted in severe and fatal postoperative complications .In group C (type-III prosthesis, n=4), all 4 dogs died within 28 days after operation. The leading causes of death were mesh exposure, collapse, infection and air leakage from implanted prosthesis itself. Conclusion: Type-I tracheal prosthesis is a suitable alternative method in reconstruction of tracheal defects, which appears very promising for the clinical application, with further improvements in promoting the epitheli alization completely.

Objective To report the surgical treatment results of cardiac and esophageal cancer of remnant stomach. Methods From 1980 to 2002, 30 patients with cardiac and esophageal cancer of remnant stomach were treated surgically. Results Primary cardiac and esophageal carcinoma of remnant stomach developed in male patients more than females. Of 30 patients, 21(70%) underwent subtotal gastrectomy with Billroth II reconstruction at first time. The mean interval between two operations was 13.5 years. Clinical symptoms frequently included abdominal distension, pain and hemorrhage. Thirty patients received radical resection. Conclusion Cancer in remnant stomach should be suspected in patients undergoing subtotal gastrectomy 5 years and over have clinical symptoms of upper gastrointestinal tract. Early diagnose and early curative resection should be done. For cardiac cancer of remnant stomach, total gastrectomy with “P” loops and Roux-en-Y esophagojejunostomy reconstruction were recommended. This procedure have advantage in retaining enough food storage preventing reflux esophagitis and allowing resection of esophagus long enough to avoid cancer remnant. For esophageal cancer of remnant stomach, colon reconstruction after the resection of esophageal cancer is recommended.

Tuberculosis (TB) is a global burden disease and is being resurrected as a major worldwide public health problem after two decades of neglect.In 1993,the World Health Organization (WHO) declared that TB had been a global emergency because of the scale of the epidemic and the urgent need to improve global tuberculosis control.China is one of the countries with the largest population,and also the top of the 22 TB high-burden countries in the world.In the United States,the longstanding downward trend in TB incidence was interrupted in the mid-to-late 1980s,where the national TB incidence peaked in 1992.Sub-Saharan Africa is one of the three regions to dominate the worldwide distribution of notified TB cases.Of the 15 countries with the highest estimated tuberculosis incidence rates in the world,13 are in sub-Saharan Africa,where HIV is the most important single predictor of tuberculosis incidence.The largest share of the global burden of HIV-related tuberculosis falls on this region.The reasons for the persisting global tuberculosis burden include increased poverty in some regions,immigration from countries with high tuberculosis prevalence,the impact of HIV,and most importantly,the failure to maintain the necessary public health infrastructure under the mistaken belief that tuberculosis was a problem of the past.Relying on currently available methods of diagnosis and treatment,the DOT strategy promoted by the WHO for global tuberculosis control is effective,affordable,and adaptable in different settings.

In order to study whether the endothelial cells (ECs) with lipid peroxidation induced by diamide can express and secrete macrophage inflammatory protein 1 alpha (MIP-1 alpha), the expression of MIP-1 alpha protein in the cells was detected by cell enzyme-linked immunosorbent assay (ELISA) and that of MIP-1 alpha mRNA was determined by cell in situ hybridization and nuclease S1 protection assay after the ECs were exposed to different concentrations of diamide for 4 h. The chemotactic activity of MIP-1 alpha was tested by micropore filter method using modified Boyden chambers. Cell ELISA showed that the expression of MIP-1 alpha protein in endothelial cells exposed to 1 mumol/L, 5 mumol/L and 10 mumol/L diamide was 1.9-fold, 2.3-fold and 1.7-fold respectively as much as that in the control cells, which was statistically significant by analysis of variance. In situ hybridization revealed that the mRNA expression of ECs treated with 1 mumol/L, 5 mumol/L and 10 mumol/L diamide was 1.3-fold, 3.0-fold and 1.7-fold as much as that in the control group, which had statistical significance (F = 188.93, P < 0.01). The mRNA expression in 5 mumol/L dimide treated ECs, measured by nuclease S1 protection assay, was 3.4-fold as much as that in the control group (t = 8.70, P < 0.05). Chemotactic response(99.50 +/- 4.31 microns) to the culture medium conditioned by 5 mumol/L diamide treated ECs, which was stronger than that(66.47 +/- 3.25 microns) conditioned by the ECs (F = 404.31, P < 0.05), was significantly decreased (F = 192.25, P < 0.05) after adding MIP-1 alpha antibody. It suggests that diamide, a lipid peroxidation inducer, could stimulate ECs to produce high level of MIP-1 alpha, and might play an important role in atherogenesis by promoting the migration of peripheral blood monocytes into arterial intima.
Cells, Cultured ; Chemotaxis, Leukocyte/physiology ; Diamide/*pharmacology ; Endothelium, Vascular/cytology ; Endothelium, Vascular/*metabolism ; Lipid Peroxidation ; Macrophage Inflammatory Protein-1/*biosynthesis ; Macrophage Inflammatory Protein-1/genetics ; RNA, Messenger/biosynthesis ; RNA, Messenger/genetics ; Sulfhydryl Reagents/pharmacology ; Umbilical Veins/cytology

Objective To find out the expression relation between TP53 and NOTCH1,and to explore their effects in head and neck squamous cell carcinoma.Methods Obtained the differentially expressed genes data of head and neck squamous cell carcinoma from 279 samples in TCGA database.Analyzed the co-expression relation between TP53 and NOTCH1 through Pearson and Spearman method.Cbioportal was used to analyze their co-expressed genes.Establish the co-expression network of TP53 and NOTCH1 with String database.The pathway and function of co-expression network was identified through KEGG and DAVID database respectively.Results Among the 279 samples,TP53 and NOTCH1 was co-expressed in head and neck squamous cell carcinoma.(Pearson score =0.45;Spearman score =0.41) There were 182 interaction pairs of TP53 and NOTCH1 related co-expressed gene according to the String database.(Pearson and Spearman score ＞ 0.3)These genes were enriched in some pathways such as T cell receptor signaling pathway,cell cycle,cell adhesion molecules and so on.These genes were enriched in some tumor related function including immune response,regulation of transposition,regulation of apoptotic process,cell cycle,regulation of GTPase activity and so on.Conclusion TP53 and NOTCH1 was co-expressed.Through establishing co-expressed network of TP53 and NOTCH1 and bioinformatics analysis,their function and signaling pathway were explored.The data generated from this study could provide a new reference in mechanism research of head and neck squamous cell carcinoma.