Objective To optimize the expression conditions(expression and induction conditions)and purification methods(non-denaturing and denaturing purification)of recombinant Hq001 protein in salivary glands of Haemaphysalis qinghaiensis.Methods The recombinant plasmid pET-30a-Hq001 was transformed into competent cells E.coil BL21(DE3),E.coil Rosetta(DE3)and E.coil ArcticExpress(DE3)pRARE2 respectively for the selection of an optimal expression strain.The final concentration of IPTG(0,0.5,1.0 mmol/L),induction temperature(20,25 ℃)and induction time(0,2,4,6,8 h)were optimized.The recombinant bacteria expressed under the ideal induction condition were homogenized by French press and the target protein was purified by passing through a Ni-NTA affinity chromatography column under either native(denaturationrenaturation-column chromatography)or denatured conditions(denaturation-column chromatography-renaturation).The purified products were analyzed by 12% SDS-PAGE.Results E.coil BL21(DE3)was proved to be the most suitable strain for the expression of recombinant Hq001 protein.The optimum induction condition was induction with 0.5 mmol/L IPTG for 4 h at 25 ℃.The target protein with a relative molecular mass of approximately 18 800 was obtained by non-denaturing purification method,and the size was consistent with the expectation.Conclusion The recombinant protein rHq001 in salivary glands of Haemaphysalis qinghaiensis can be obtained by the optimized expression conditions and purification methods.

OBJECTIVE:To provide a reference for the management of the facilities and equipment of the pharmacy intrave-nous admixture service(PIVAS). METHODS:The experience in the management of the facilities and equipment of PIVAS in our hospital was introduced in respect of system establishment,archives management and the maintenance of important equipment,and the performance of core purification equipment was tested to evaluate management effect. RESULTS & CONCLUSIONS:The PIV-AS in our hospital established a practical system by developing and improving 7 management systems,9 sets of operational proce-dures and 5 contingency plans,increased the awareness of archives management by perfecting 27 archive files and standardized and conducted the maintenance of key equipment. In 2014 and 2015,after implementing management,the passing rates of all test items of the purification equipment were 100%. It is suggested that facilities management should focus on active preparation of an intelli-gent monitoring system,the improvement of the information system and the training of professional management personnel.

Objective To study the genetic toxicity of 3 brands of detergents to the root tip cells of Vicia faba in the inducing time and the inducing way(continual mutagenesis,accumulative mutagenesis) with micronucleus and the chromosomal aberration Methods The root tip cells of Vicia faba were taken as the subjects,the genetic safety tests were conducted on those materials treated with detergent of a certain concentration(0.25%) for 2、4、6 h of continual mutagenesis and accumulative mutagenesis respectively.Distilled water was used as the control.Results The continual mutagenesis showed that all the three detergents could induce the root tip cells of Vicia faba to produce micronucleus and chromosomal aberration and as the mutagenic time prolonged,at 6 h,the changes became weak.The genetic toxicity in the accumulative mutagenesis group was greater than that in the continual mutagenesis group.Conclusion According to the results of the present paper,to some extent,detergents may produce genetic toxicity.

Objective To know the risk factors of indoor environment associated with childhood asthmas. Methods Using 1∶1 matched case-control study design,140 cases and 140 age-sex-matched controls from the Affiliated Hospital of Ningxia Medical University were investigated for the indoor environment risk factors,from July 2004 to May 2008. Conditional Logistic regression model was used for data analysis. Results It was revealed in univariate and multivariate conditional Logistic regression analysis that there was significant correlation between using coal for cooking,newly indoor decoration,raising pets and the childhood asthma with odds ratios of 1.67,6.46 and 3.06 respectively(P

Objective: To study and compare the views on cancer associated information and communication needs of cancer patients among cancer patients, their relatives, doctors and nurses, and other groups of people. Methods: A total of 501 cancer patients, 169 relatives,86 doctors and nurses, 176 healthy elderly people and 505 first grade college stu- dents were asked to complete the Information Preference Questionnaire of Cancer Patients and 2 questions regarding gen- eral information amount and content (IPQCP+2), and the Information Need Questionnaire (INQ). The elderly and the stu- dents were asked to complete two sets of IPQCP+2 and INQ in two roles: one as themselves and the other as patients. All data were analyzed in detail. Results: The views of different groups are significantly different. The differences include the viewpoints on whether to disclose the diagnosis of cancer and to inform the life expectancy to cancer patients, and whether the patients should participate in decisions about their medical care and treatments. The mean score of IPQCP on the over- all items and the 4 dimensions of all groups, and their opinions on general information amount and content for patients are significantly different. Conclusion: The views of different groups of people on cancer associated information and commu- nication needs of cancer patients are different. The views and requests of cancer patients themselves are the basis of doc- tor- patient communication. The prevention of cancer should be based on the characteristics of different groups of people and the patients’views should be considered.

Objectives: To develop and evaluate the Psychological Adjustment Scale for Cancer Patients (PASCP).Methods: A self-report rating scale was developed with structured methods. A total of 557 cancer patients were asked to complete the scale and evaluate the importance of each item. And 183 relatives and 96 doctors and nurses were also asked to evaluate the importance of each item. The scale was refined and its reliability, validity and responsiveness were evaluated. Results: (1)PASCP was finally refined as a scale with 36-item, 5 dimensions including the impact on emotion/self-esteem, self-perception, daily life, relationship with others/social life, others, and an item to value the general psychological adjustment after the patients were diagnosed with cancer. (2)The scale had good content validity. Its construct was consistent with the concept designed. The factor loading spectrum and ANOVA are 0.63-0.79 and 7.44, 0.67-0.76 and 5.42, 0.69-0.88 and 4.77, 0.66-0.91 and 4.77, 0.63-0.80 and 4.38 respectively. The criterion validity with HAD and FLIC were 0.78 and 0.69 respectively. (3)The test-retest coefficients for the 5 dimensions and the total score were 0.83、0.82、0.86、0.78、0.76 and 0.89 respectively;Cronbach ? for them were 0.83, 0.86, 0.80, 0.78, 0.69 and 0.89 respectively. The split-half reliability for the scale was 0.93. 4.The scale could reflect the changes of the patients' psychological adjustment before and after treatment and recovery. Conclusion:PASCP has high validity, reliability and responsiveness. It is applicable in clinical practice in China.

Objectives:To study the satisfaction with doctor-patient communication among cancer patients, their relatives and medical staffs, and its associated factors.Methods:A total of 423 cancer patients were asked to rate their satisfaction with information communication, to complete the Information Need Questionnaire (INQ), the Demographic Questionnaire (DQ), and 4 questions regarding general information amount and content the patients got and wanted respectively. 82 relatives, 86 medical staffs were also asked to rate their satisfaction with information communication.Results:A certain proportion of cancer patients, their relatives and medical staffs were not very satisfied with their information communication. There are significant differences among them. The satisfaction of medical staffs was less. It suggests that they want to improve their communication level. Satisfaction with information communication of cancer patients was affected by the contents and styles of information communication, and whether their communication experiences met their preferences.Conclusions:Before providing information to cancer patients, we need to consider the style they want and to find out their needs and preferences.

Objective To evaluate the diagnosis and therapy of solid pseudopapillary tumor of the pancreas (SPTP).Methods From January 2006 to October 2011,the clinicopathological data of 24 SPTP patients diagnosed and surgically treated at Tianjin Medical University Cancer Hospital were analyzed retrospectively.Results There were 22 women and 2 men,the median age was 27.5 years old,with a range from 12 to 51 years.The most common tumor localization were at the head of the pancreas ( 11 patients,45.7％ ),followed by the body (5 patients,20.8％ ),the tail (4 patients,16.7％ ),the body and the tail (3 patients,12.5％ ) and the neck (one patient,4.2％ ).Clinical symptoms were nonspecific and the preoperative diagnosis of SPTP depended on imaging study such as ultrasonography,CT and MR.All patients were treated surgically and the correct diagnosis was made according to pathology after surgery.In a follow-up ranging from 2 to 55 months( median 27.2 months),23 cases were alive with no evidence of disease recurrence,one patient was alive with tumor recurrence.Conclusions SPTP was a less common neoplasm with low-grade malignancy,occurring mainly in young women.Complete tumor resection results in satisfactory prognosis.

Objective To explore the expression of 10 kDa interferon-gamma-induced protein(IP-10)in pathological scar and its role in the pathogenesis of pathological scar.Methods Imrnunohistochemistry was performed to detect the expression and distribution of IP-10 in 28 patients with keloid(group K),34 patients with hypertrophic scar(group HS),and 20 normal controls(group N).The data were collected and analyzed statistically.Results The expression of IP-10 was significantly higher in groups K and HS than in group N(P＜0.01),but no significant difference in the expression of IP-10 was found between groups K and HS.Conclusion IP-10 may enhance the formation of pathological scar by attracting T lymphocytes and inducing immune/inflammatory response.

BACKGROUND: Most animal experiments of transgene are derived from mice; therefore, it is necessary to establish a focal cerebral ischemiareperfusion model and significant to prevent and cure ischemic cerebrovascular diseases.OBJECTIVE: To establish a convenient and reliable model with middle cerebral artery occlusion reperfusion (MCAO/R) in mice.DESIGN: Randomized controlled animal study.SETTING: Institute of Cerebrovascular disease, Affiliated Hospital of Medical College of Qingdao University.MATERIALS: Twenty healthy BALB/c mice, of both genders, weighing 25-30 g, of SPF grade, were divided into sham operation group (n=5), ischemia group (n=10) and 22-hour reperfusion group (n=5) on the basis of digital table. In addition, according to digital table, 130 healthy male Kunming mice were divided into sham operation group (n=10), 24-hour ischemia group (n=30), 2-hour ischemia/22-hour, 46-hour and 70-hour reperfusion groups with 30 in each group; meanwhile, 30 female mice were divided into sham operation group, 24-hour ischemia group and 2-hour ischemia/22-hour reperfusion group with 10 in each group. All Kunming mice were weighing 25-30 g and of SPF grade.METHODS: The experiment was carried out in the Institute of Cerebrovascular Disease, Affiliated Hospital of Medical College of Qingdao University from July 2005 to March 2006. The 6-0 suture with silica gel covered at an end was sent from the left external carotid artery (ECA) into internal carotid artery (ICA) till arriving at the initiation of middle cerebral artery (MCA) to block the blood stream in it, then drawing the suture from ICA 2 hours after occlusion to accomplish reperfusion. Mice were cut off their heads in sham operation group at 24 hours after operation, in ischemia group at 24 hours after blocking blood stream and in reperfusion group at 24, 48 and 72 hours after operation. Reliability of models was evaluated with neurology score and tetrazolium chloride stain. Longa standard scores: neurology score ≥ 1 point was regarded as successful models;coronal sections of brain tissue were stained with tetrazolium chloride, and the white region was regarded as infarcted volume.MAIN OUTCOME MEASURES: Neurology score and infarcted volume after staining of triphenyltetrazolium chloride in brain tissue.RESULTS: All mice were involved in the final analysis. ① Successful rate was 20% of BALB/c mice, 66.7%-73.3% of male Kunming mice and 40%-50% of female Kunming mice. ② Brain sections of BALB/c mice in sham operation group were orange at both sides of cortex and infarction focus was not observed. A big infarcted volume was observed on brain sections of mice in ischemia group, and infarcted volume counted for 50%-70% as homonymy hemisphere on optochiasmatic coronal sections. The condition of Kunming mice was similar to that of BALB/c mice, but infarcted volume counted for 40%-65%. In addition, condition in ischemiareperfusion group was similar to that in ischemia group. A big infarcted volume was observed on brain sections, and infarcted volume counted for 50%-75% as homonymy hemisphere on optochiasmatic coronal sections.The condition of Kunming mice was similar to that of BALB/c mice, but infarcted volume counted for 40%-65%.CONCLUSION: The model with MCAO/R in mice characterizes by relatively smaller trauma, and the ischemic region is stable; therefore, it can be used to accurate timing control of ischemia/reperfusion. This model is an ideal one for researching pathophysiological changes, prognosis and therapy in cerebrovascular disease.