Objective To investigate the effect of using prophylactic antidepressant treatment with citalopram on motor function recovery in patients with acute cortical infarction. Methods The patients with first-ever acute middle cerebral artery territory cortical infarction and with upper limb motor deficits within 24 h after onset were enrol ed prospectively. They were randomly divided into either a citalopram group or a control group. On the basis of conventional treatment, the citalopram group was given citalopram hydrobromide tablets oral y (20 mg/d) within 48 h after onset of stroke for 30 d. The 17-item Hamilton Depressive Rating Scale (17-item HDRS) was used to conduct the assessment of depressive symptoms before and after treatment. The National Institutes of Health Stroke Scale ( NIHSS ) and Fugl-Meyer Assessment (FMA) hand motor function score were used to conduct function assessment before and after treatment. Results A total of 66 patients were enrol ed in the analysis, including 32 patients in the citalopram group and 34 in the control group. There was no significant difference in baseline clinical data between the 2 groups. The baseline stroke severity of al patients was moderate (NIHSS score 5-15). Compared with baseline level at day 30 after the treatment, the HDRS score of the citalopram group was improved significantly (6. 70 ± 3. 58vs. 9. 86 ± 3. 48; t= 3. 380, P= 0. 001 ). However, there was no significant difference with the control group after treatment (6. 70 ± 3. 58 vs. 8. 12 ± 2. 96;t=1. 745, P=0. 086). The NIHSS scores in the citalopram group and the control group were decreased respectively (4. 30 ± 1. 88 vs. 2. 00 ± 1. 24;t=5. 900, P<0. 001). The hand FMA scores were increased significantly (4. 00 ± 0. 70 vs. 1. 42 ± 1. 91;t=7. 197, P<0. 001). There was significant difference between the 2 groups. In addition, the hand motor function score at day 30 after treatment was more than 25% of the baseline recovery in 14 patients of the citalopram group (43. 8%) and in 5 of the control group (14. 7%). The citalopram group was significantly higher than the control group (χ2 =6. 783, P=0. 009 ). Conclusions Early prescription of citalopram can significantly promote the motor function recovery in acute cortical infarction patients with moderate motor deficit and its mechanism may not be associated the antidepressant effect.

Objective To evaluate the diagnostic value of multi-slice spiral CT urography(MSCTU)in pyelic and uretal disease.Methods 46 cases of pyelic and ureter disease with integrated clinical data were collected in two years,all cases were undergone multi-slice spiral CT contrast-enhanced scanning,the excretory-phase images were transferred to the workstation and shaded volume rendering(SVR),maximun intensity projection(MIP),multiplanar reconstruction(MPR)and curved planar reconstruction(CPR)were performed.Results In 46 cases,16 pyelic and ureteral calculus,12 congenital deformitions(including pelviureteric duplication malformations,retrocaval ureters,ureteropelvic junction stenosis,supern-umerary kidneys),5 inflammatory stenosis,7 primary pyelic and ureteral cancer,4 ureters were involved by bladder cancer and 2 compressive lesion were found.Conclusion MSCTU has obvious advantage in the diagnosis of pyelic and uretal disease.

Objective To estimate the ability of MSCTA in displaying internal mammary artery . Methods MSCTA images of clinically suspected aortic disease in 30 patients were retrospectively re-processed and analyzed.The transverse images were combined with the reorganization images to observe the running rule,length and diameter of internal mammary artery.Results 60 internal mammary arteriae were showed by MSCTA,one was maldevelopment and obvious smaller than that of the contralateral one,four in two patients were obvious circuitous and two in one patient appeared abnormal branch.All internal mammary arteries originated from the outside of the first segmental of the left and right subclavian artery respectively,and descended along the back of the upper 6 sostal cartilages,in the distance of 2 cm to the limbus of sternal border,then divided into two in the sixth intercostal space level.56 on anterior view showed straight line descent,while on lateral view,they closed to the costal cartilage and were mild-circuitous.The average length of internal thoracic arteries was:(20.11?1.85) cm in right and (20.34?1.57) cm in left, the average diameter was:(2.49?0.37) cm in right and (2.45?0.35) cm in left.Conclusion MSCTA can clearly demonstrate the internal mammary artery,which provides various kinds of information for the clinical diagnosis and treatment of certain diseases.

Objective To estimate the value of MSCTA in the diagnosis of subclavian arterial stenosis.Methods 10 patients with suspected subclavian steal syndrome which causes subclavian artery stenosis underwent MSCTA, the contrast medium was Iohexol 80~90 ml, the flow rate was 3~4 ml/s with sure-star techniques,excretory-phase images were transferred to the workstation and shaded volume rendering (VR),multiplanar reconstruction(MPR),and curved planar reconstruction (CPR) were performed.The left subclavian artery was divided into proximal and distals by openning of left vertebral arteries. Results The narrowing positions:6 cases were in proximal and 4 cases were in distal of subclavian artery.6 cases were focal stenosis,4 cases were multiple stenosis and totally 14 focus.The extent of stenosis was 0.8~2.0 cm,10 cases were ≤1 cm and 4 cases were 1.1~20 cm.The degree of stenosis included 7 cases ≤50%,3 cases in 50%~70%.The results was same comparing with that of DSA.Conclusion MSCTA plays an important role in diagnosing subclavian arterial stenosis.

Objective To evaluate the value of multi-slice spiral CT(MSCT) post-processing technique in diagnosis of spinal injury. Methods CT materials in 44 cases of spinal injury were retrospectively analyzed,all case were scanning with 16-slice helical CT,the images were passing on an independent workstation for MPR and SVR technique,and compared with axial 2D imaging of CT . Results MPR and SVR images could show the fracture line and displacement of fractured osseous fragments and spatial relationship of structure clearly,MPR images could show small fracture line and dislocation of joint actually. Conclusion MPR and SVR images are important supplement to axial 2D images,the axial imaging combined with various reconstruction technique can provide the space structure of spinal injury ,and play an important role in selecting therapy project and evaluating prognosis.

Objective To evaluate the value of multi-slice spiral CT(MSCT)post-processing technique in diagnosis of small peripheral lung cancer .Methods CT materials with small peripheral lung cancer in 20 cases were retrospectively analyzed ,after 16-slice spiral CT scanning ,both plain and contrast ,and reconstructed using 1mm slice thickness ,the images were passing on an independent workstation for SSD and MPR technique .Results the images of SSD and MPR could reveal the relation between focus and surrounding organs in different dirctions,also revealed the features of pulmonary carcinoma stereos copically and directly,could show actually lobulated sign ,burr sign,pleural indentation sign and vessel convergence sign with small peripheral lung cancer ,the SSD imaging was superior in judging the presence of the lobulated sign .Conclusion It is very valuable for diagnosis of small peripheral lung cancer that application of SSD,MPR and two dimensional reconstruction。

With bibliometrics method,the 60 published issues from 2011 to 2015 of Chinese Journal of Laboratory Medicine were analyzed from the following aspects:the ratio of fund papers,sources and the number of funds,the collaborating rate of co-authors and the cooperation degree,to reveal the overall level of found paper in medical field.

AIM: Tranditional methods of screening drugs for benign prostatic hyperplasia(BPH)requires senile male animals such as dogs or rats.It consumes a long time to get the results. Over-expression of type Ⅱ5α-reductase in prastate induces BPH.A fast and efficient screening model of type Ⅱ5α-reductase inhibitors for BPH was set up in this paper.METHODS:Microsomes were extracted from male Sprague-Dawley rat livers by gradient centrifugation.Type Ⅱ5α-reductase enzyme-catalyzed reaction was assayed by UV-spectrophotometry using testosterone as a substrate and NADPH as hydrogen donor.The change of enzymatic activity was recorded with a NADPH wavelength of 340 nm by subtracted descending velocity of the control(without 5α-reductase).Effects at different conditions(temperatures,pH,enzyme and testosterone concentrations)on 5α-reductase were assayed.RESULTS:The suitable condition of type Ⅱ5α-reductase reaction Was defined as concentration of 109.05 mg protein/L enzyme(pH 6.00)with 2 μmol/L testosterone at 37℃.Michaelis'constant of type Ⅱ5α-reductase was 0.6μmol/L.Finasteride,a new drug for BPH,significantly inhibited activity of type Ⅱ 5α-reductase.IC50 of finasteride Was 64.1 nmol/L.As solvent of drugs,concentration of ethanol below 1.1% did not inhibite enzymatic activity(P＞0.05).Concentration of ethanol above 1.6%could obviouslv suppress enzymatic activity(P＜0.01).Daytime difference within five days had no significant difference(P＞0.05).CONCLUSION:A handy and fast screening method for type Ⅱ 5α-reductase inhibitors has been set up using UV-spectrophotometry.It may be used to screening drugs for BPH treatment.

0.05).Concentration of ethanol above 1.6 % could obviously suppress enzymatic activity(P0.05).CONCLUSION: A handy and fast screening method for type Ⅱ5?-reductase inhibitors has been set up using UV-spectrophotometry.It may be used to screening drugs for BPH treatment.

AIM: To prepare α-tricalcium phosphate (α-TCP)/calcium polyphosphate (CPP) fiber and to study the feasibility of CPP fiber to reinforce calcium phosphate bone cement composites. METHODS: Firstly,α-TCP powder was synthesized using chemical sediment method. Secondly, the α-TCP was mixed with CPP fiber according to different contents and lengths. Finally, bone cement was tempered with firming agent. Solidification time and mechanical property of the samples were measured. Microstructure of hardened sample was observed with scanning electron microscope. RESULTS: When the amount of CPP fibers was 10% and the length was 2 mm, the compressive strength reached 62.5 MPa and the rupture strength reached 12.4 MPa. Scanning electron microscope suggested that CPP fibers with great associativity were well distributed in bone cement. After immersing in Ringer fluid for two months, the CPP fibers did not biodegrade obviously and still had certain function to increase strength and toughness. CONCLUSION: To a certain extent, the CPP fiber can increase strength and toughness of bone cement. Furthermore, α-TCP/CPP composites have good mechanical properties and biocompatibility.