Objective To evaluate the clinical effects of magnetic attachment on Kennedy Ⅰdentition defect.Methods Totally thirty cases of Kennedy Ⅰ dentition defect were enrolled.Magfit EX 600 magnetic attachments were applied as the extra-coronal retainer on abutment,meanwhile the guide plane and key-key way were applied as the stablizinge apparatus for dentures.The removable partial dentures with magnetic attachment were fabricated in casting framework way.After the clinical examination,X-ray and patients' subjective feeling interview,the satisfaction status to the dentures was evaluated.Results Totally thirty patients were followed up for a period ranging from 1 to 3 years.25 patients were satisfied with the dentures in both function and esthetic results.Additionally,one patient was found to have gingivitis,and 2 patients were found the attachments loosened.These 3patients were considered basically satisfied subjects.The overall satisfaction rate was 93.3％.The rest 2 patients were found alveolar bone absorption,which led to base plate of molar teeth region sinking,and partial plastic base plate of denture cracked or damaged.Therefore the 2 patients were considered as unsatisfied subjects.Conclusions The removable partial dentures with magnetic attachment not only exhibit characteristics as compact,aesthetic and comfort,but also supply reliable retention,stablization,excellent masticatory efficiency and enough protection for abutments in patients bearing Kennedy Ⅰ dentition defect.

Objective To analyze and evaluate the related data of bottom clearance in Fuling section of Three Gorges Reservoir. Methods Bottom clearance in Fuling section of Three Gorges Reservoir was carried out in Fuling district during the period from May to December in 2002. All the data related to clearance were collected before and after the bottom clearance. Based on the collected data, the effect of clearance was assessed. Results 5 882 general pollution sources, 68 contagion pollution sources were cleaned in Fuling district, the clearance rates of both were 100%. The rat densities in agriculture areas and the dismantling cities were 3.97% and 2.97% respectively before rat extinguishment, then became 0.65% and 0.33% after rat extinguishment. Conclusion Through the bottom clearance of Three Gorges Reservoir, contagion and general pollution sources in the emigrant areas have been cleaned up, so water pollution in Three Gorges Reservoir and the rats mediated diseases have been prevented and controlled.

Aim The m_5AChR-G_ 11? fusion protein was expressed by baculovirus-Sf9 cells system, then using it to identify the specific agonists and antagonists for m_5AChR via detecting the affinity of GDP and m_5AChR-G_ 11?. Methods The m_5AChR-G_ 11? fused cDNAs were generated via a two-step PCR protocol and inserted into pBacPAK9 virus vector. We expressed m_5AChR-G_ 11? fusion protein and m_5AChR protein using baculovirus-Sf9 cell system. [ 3H]QNB and [ 35S]GTP?S binding tests were performed to detect the expressional level of receptor proteins and determine the affinity of GDP and m_5AChR-G_ 11? fusion protein. Results The expression level of m_5AChR-G_ 11? was (47.6?3.2) nmol?g -1 protein. The affinity of GDP to G_ 11? partner changed in the presence of different muscarinic ligands. IC_ 50 values of GDP in the presence of ACh, YM796, Oxotremorine, Methixene, Dextimide and atropine were 128.0, 72.1, 68.5, 16.2, 14.9 and 9.7 ?mol?L -1 respectively, and that in the absence of muscarinic ligand was 20.8 ?mol?L -1. Conclusion The m_5AChR-G_ 11? fusion protein has the pharmacological specificity of M_5 receptor and the efficient coupling interaction of the two partner. Affinity of GDP to ligand bound m_5AChR-G_ 11? fusion protein represents the species of muscarinic ligands. ACh is a full agonist for m_5AChR-G_ 11? fusion protein, YM796 and oxotremorine are partial agonists, while methixene, dextimide and atropine are antagonists.

Objective:This work aimed to compare the three-year results, prognostic analysis, and adverse reactions of intensi-ty-modulated radiation therapy (IMRT) positioned by PET-CT and conventional radiotherapy in locally advanced non-small cell lung cancer (NSCLC) patients who underwent concurrent chemotherapy. Methods:A clinical trial was carried out in Qingdao Cancer Hospi-tal. The patients who joined our study were divided into IMRT and conventional radiotherapy (CRT) groups. A total of 48 patients were in the IMRT group and another 40 were in the CRT group. The total dose was 60 Gy throughout the 6-week treatment. The plati-num-based concurrent chemotherapy, combined with regimens such as docetaxel, navelbine, and pemetrexed, was conducted for two cy-cles followed by two to four cycles of consolidation chemotherapy. Results:Significant differences were observed between the curative ratio of the IMRT (77.1%) and CRT (52.5%) groups (P=0.015). No significant difference existed between the short-term survival rates of the two groups. The 1-, 2-and 3-year survival rates were 77.1%, 54.2%, and 22.9%in the IMRT group, as well as 65.0%, 47.5%, and 17.5%in the CRT group, respectively. Significant differences existed in some adverse reactions between the two groups, such as radia-tion gastrointestinal reactions, esophagitis, and pneumonia. More patients died of local recurrence and radiation pneumonia in the CRT group than in the IMRT group. The local recurrence rate was also lower in the IMRT group than in the CRT group. Conclusion:IMRT has obvious advantages in improving the short-term curative effect and reducing adverse reactions. Regarding the survival rate, a long-term follow-up of the two groups is required in the future.

Objective Explore the individual difference in carbon monoxide (CO) poisoning and analyze whether females were be more resistant to CO poisoning.Methods We collected 207 cases of CO poisoning deaths, and then we analyzed the Carbonyl Hemoglobin (COHb) results sorted by different sources of CO.Results The source of CO had significant impact on the fatal COHb level, but gender was not significant in affecting COHb level. In addition, it is possible that children and elders were less resistant to COHb than adults.

BACKGROUND:BIOSSN4 nickel-free stainless steel is an austenitic medical stainless steel material, which has passed the standard hemolysis test, cytotoxicity assays and sensitization test of the National Institute for the Control of Pharmaceutical and Biological Products. OBJECTIVE:To evaluate theinvitro cytotoxicity and corrosion resistance of a new medical BIOSSN4 nickel-free stainless steel. METHODS:The L929 mouse fibroblasts suspension was seeded in 96-wel plates at a concentration of 1×108 /L, and were divided into five groups. BIOSSN4 nickel-free stainless steel extract, 316L stainless steel extract, gold aloy extract, lead material extract (positive control) and RPMI1640 medium (negative control) were added respectively. After 1, 2 and 3 days of culture, cel morphology was observed. The absorbance value in each group was determined using MTT assay. The relative cel proliferation rate was calculated. Toxicity grading was evaluated. In the simulated oral environment, the eletric potential of corrosion, current density of corrosion and polarization resistance of BIOSSN4 no-nickel stainless steel, 316L stainless steel and gold aloy were determined. RESULTS AND CONCLUSION:Within 3 days of culture, in lead material extract group, cels shrunk; the number of cels significantly reduced; the relative growth rate was lower than that in the other four groups (P < 0.05). In the other four groups, the cel morphology was good, and the relative growth rate was over 75%. The toxicity of BIOSSN4 nickel-free stainless steel extract, 316L stainless steel extract and gold aloy extract was grade 1. The toxicity of lead material extract was grades 2-3. These results demonstrate that BIOSSN4 nickel-free stainless steel has good biocompatibility. The corrosion resistance of BIOSSN4 nickel-free stainless steel is higher than that of the 316L stainless steel but lower than that of the gold aloys.

Objective To assess the sensitivity and specificity of vaginitis pathogen detection reagent kit (Nucleic acid hybridization). Methods Four hundreds cases of vaginal secretion samples were detected with Amsel, vaginalis culture, fungal culture and Affirm VPIII detection method, respectively. Using the methods of Amsel, vaginalis culture and fungal culture as the gold standard, the clinical application value of Affirm VPIII detection method was evaluated. Results Compared with Amsel, the sensitivity and specificity of Affirm VPIII detection was 92.2%and 70.5%, respectively. Compared with fungal culture, the sensitivity and specificity was 88.3% and 92.9%, respectively. Compared with vaginalis culture, the sensitivity and specificity was 92.6% and 93.8%, respectively. There was a good consistency between the gold stardard and the Affirm VPIII detection. Conclusion Compared with the traditional detection methods,the Affirm VPIII detection has the advantages of fast detection speed,simple operation, and high sensitivity and specificity. In addition, it can identify three kinds of vaginitis pathogenic microorganisms at the same time,with a certain clinical value.

Objective To evaluate the validity and reliability of simplified Chinese version Scoliosis Research Society-22 (SRS-22) questionnaire on assessment of life quality of adolescent idiopathic scoliosis (AIS) patients at different time points pre-and post-operation. Methods Translation and cross-cultural adaptation of the English version of SRS-22 questionnaire was done. 177 AIS patients were involved in present study and the life quality before and 6-and 12-months after operation was assessed by SRA-22 and Short Form-36 (SF-36) questionnaire, of them 145 cases (26 males and 119 females; 11.1-17.9 years old, averaged 15.8; Cobb angle 41?-109?, averaged 63?) completed all the 3 steps. To evaluate the reproducibility, 65 patients were assessed twice in an interval time of 4-6 days (averaged 4.6 days) before operation and 55 valid questionnaires were received, 65 patients were assessed twice in an interval time of 7-21 days (averaged 14.7 days) at 12 months following up and 57 valid questionnaires were received. The two measures of reliability as internal consistency and reproducibility were determined by Cronbach's ? coefficients and intraclass correlation coefficient (ICC), respectively. Concurrent validity was measured by comparing with SF-36 questionnaire. Measurement was made using the Pearson correlation coefficient (r). Results The Chinese version SRS-22 questionnaire had a satisfactory validity and reliability, with average Cronbach's ? coefficients of 0.79-0.85 and average ICC of 0.77-0.87. Considering concurrent validity, 2 domains had excellent correlation (r=0.75-1.00), while 12 had moderate correlation (r=0.50-0.75). The function/activity (SRS-22) and role-physical or physical functioning (SF-36), pain (SRS-22) and pain index (SF-36), mental health (SRS-22) and mental health (SF-36) had good correlation (r=0.70-1.00). For the 145 patients, there were some differences in 5 various domains between the three measurements (pre-operation and 6 and 12 months post-operation). The scores of all domains 12 months after operation increased significantly than those of pre-operation and 6 months post-operation (P

Objective To evaluate the potential mutagenicity of a new machinable bioactive glass-ceramic material.Methods Thirty N1H mouse inbred line (female:male =1:1) were divided to three groups at random (n =10),including glass-ceramic groups (oral administration of 5 g/kg glass-ceramic powder and arabic gum),negative control group (arabic gum in equal volume),and positive control group (oral administration of 40 mg/kg cyclophosphamide).The mice orally intook the equivalent liquor and were sacrificed with bone marrow cells abstracted 24 hours later.The micronucleated cells were counted in 1 000 polychromatic erythrocytes (PCE) per mouse,then the rate of the micronucleus in every group was measured.Results The rate of the micronucleus in glass-ceramic group,negative control group and positive control group was 1.31±0.53‰, 1.32±0.62‰ and 29.20±0.74‰ respectively.There was no significant difference in the rate of the micronucleus between the experimental and negative groups (P＞0.05),while a significant difference in the rate of the micronucleus was observed between experimental and positive groups (P＜0.01).Conclusion The new machinable bioactive glass-ceramic materials couldn't increase the micronucleus rate of mouse bone marrow cells.