AIM: To establish the determination of ? eudesmol in atractylis oil from Rhizomes of Atractylodes Lancea (THUNB.) DC. by gas chromatography. METHODS: Pentadecanol had been used as the internal standard substance in internal standard method. The GC system consisted of capillary column, 10%SF 30 as the stationary phase, nitrogen as the carrier gas, and FID as the detector. RESULTS: Both ? eudesmol in essential oil and pentadecanol had got satisfactory separation under the chromatographic condition. The mean recovery of ? eudesmol was 99.60%, and RSD was 1.30%. CONCLUSIONS: The method is sensitive, accurate and reproducible, and it can be used to control the quality of the essential oil from Rhizomes of Atractylodes Lancea (THUNB.) DC.

Objective: To study the influence of Ruhuang(Rhubarb and Lactohacillin) preparation to endotoxin and intestinal defence function of cirrhotic rats.Methods:88 male Wister rats were randomly divided into normal group,model group,prevention group,prevention of control group,model treatment group and model treatment of control group.Liver cirrhosis model was induced by CCl4 combined with other factors,Ruhuang preparation was used for the prevention and treatment,lactulose was used as positive control drug.The level of plasma endotoxin,intestinal mucosal S-IgA and serum D-lactic acid were detected.Results : The blood plasma endotoxin level of Ruhuang prevention group and model treatment group is lower than that of model group obviously(P

This study is to report the establishment of an UPLC-MS/MS method for the determination of plasma concentration of UA carried in self-microemulsifying drug delivery system (SMEDDS) and its pharmacokinetics in rats. It was used for determination and analysis when serum with internal standard was extracted from C18 solid-phase column. Acquity UPLC BEH C18 column (100 mm x 2.1 mm, 1.7 microm) was used for separation. The mobile phase was acetonitrile -0.1% ammonia with gradient elution at the flow rate of 0.2 mL x min(-1). The column temperature was 40 degrees C and the detection wave length was 210 nm. It was detected by negative ion using electrospray ionization source (ESI) and scanned by multiple reaction ion monitoring (MRM) mode. The liner relationship of UA was very good in the range of 1.19-3 815.00 ng x mL(-1) (r = 0.999 0). Recovery rate of different concentrations were 87.42%-89.95%. The precision of inter-day and intra-day were less than 11%. The method developed in our study was proved to be sensitive, rapid and simple. It is suitable for the pharmacokinetic study of UA-SMEDDS in rats.

This study aimed at developing an ultra-performance liquid chromatography electrospray time of flight-mass spectrometry (UPLC-ESI-QTOF-MS) method for the identification of bovine hide gelatine from the shell glue of the red-eared slider.Tortoise shell glue was made from the shell of red-eared slider or Reeves' turtle (Chinemys reevesii).The samples were analyzed on UPLC-ESI-QTOF-MS after being digested by trypsin,and the ions at m/z 604.8,m/z 641.3,m/z 790.8 of the marker peptides of bovine hide gelatin were detected.As a result,it was found that the test results of the ions made from the shell of red-eared slider at m/z 641.3 were positive,taking the same fragmentation regularity of MS/MS as the standard of bovine-hide gelatine,while negative results were detected from the ions made from the shell of Reeves' turtle.In conclusion,the method was simple and accurate provided a basis for distinguishing the tortoise shell glue from bovine hide,which could be also used for the quality control of tortoise shell glue.

This study aimed at identifying pig-hide gelatin from tortoise shell glue using ultra-performance liquid chromatography-electrospray-time of flight mass spectrometry(UPLC-ESI-QTOF-MS/MS).The samples of tortoise shell glue were digested by trypsin before the analysis on UPLC coupled with QTOF mass spectrometry.The ions at m/z 925.4 were selected as the marker peptides of pig-hide gelatin.It was found that the test results of the ions at m/z 925.4 were positive in the samples with low contents of the marker peptides of tortoise shell glue,indicating the existence of pig-hide gelatin.However,the result of bovine-hide gelatinordonkey-hide gelatin was negative,while negative results were detected in tortoiseglue standard.The finding was in conformity with previous references over the fragmentation regularity of MS/MS.In conclusion,the method can be adopted to identifying the pig-hide gelatin from tortoise shell glue and applied to the quality control of tortoise shell glue with high specificity and sensibility.

OBJECTIVETo determine the contents of total saponin in the unprocessed Semen Platycladi and defatted powder of Semen Platycladi.

METHODThrough contrasting different coloration, proper condition was selected by using orthogonal test design. Spectrophotometry was established to determine the contents of total saponin in the unprocessed Semen Platycladi and defatted powder of Semen Platycladi.

RESULTColoration system of vanillin-acetic acid-sulfuric acid was determined. The proper condition of coloration was that sulfuric acid was 80%, water bathed 85 and heated for 10 minutes. The contents of total saponin in unprocessed Semen Platycladi was 0.142% the content of total saponin in the defatted powder of Semen Platycladi was 0. 319%.

CONCLUSIONThis method, stable, simple and quick, is applicable to the determination of total saponin in Semen Platycladi. A Little of total saponin is lost in the processing course of Semen Platycladi.

Cupressaceae ; chemistry ; Fatty Acids ; chemistry ; isolation & purification ; Powders ; Saponins ; analysis ; Sensitivity and Specificity ; Temperature ; Time Factors

Aim To investigate the anti-angiogenesis and anti-xenograftes of UA in zebrafish larvaes. Meth-ods 24 hour post-fertilization ( hpf ) TG zebrafish was treated with different concentration of UA for 24h, and the number of intersegmental vessels( IVS) were detec-ted under fluorescent microscope respectively;then the models of AB/TG zebrafish xenografts were established by be micro-injected with SMMC-7721 or HT-29 cell at 48hpf respectively, and after cocultured with UA for 48h, optical density (OD) of the SMMC-7721 cell and subintestinal veins ( SIVs) induced by HT-29 were e-valuated under confocal microscope. Results ISV as-say showed that UA could cause IVS missing or disap-perance, inhibition ratio reaching 20. 25% and 26. 65%. UA blocked the spread of SMMC-7721 cells in zebrafish and OD value,and inhibition ratios at three concentrations were 38. 01%, 54. 69%, 61. 88%, re-spectively; in another SIVs assay induced by xeno-grafts, UA at concentration 10 and 15mg·L-1 showed that SIVs were inhibited (P<0. 01) obviously. Con-clusion UA could inhibit the angiogenesis and the growth of SMMC-7721/HT-29 xenografts,and the anti-tumor mechanism may be related with VEGFR2 expres-sion.

Objective To investigate the correlation between the infarction location and progressive motor deficits (PMD) occurrence.Methods The patients with middle cerebral artery(MCA) infarction within 24 h of onset without thrombolytic therapy were included.The National Institutes of Health Stroke Scale(NIHSS) motor item score increase ≥2 points of the base line within 7 d after stroke onset served as the PMD diagnostic criteria.The differences in clinical and laboratory data,and infarction location were compared between the PMD group and non-PMD group.The multivariate Logistic regression analysis predicted the risk factors of PMD occurrence.Results A total 121 patients with MCA acute cerebral infarction were included in the study and divided into the PMD group (45 cases) and non-PMD group (76 cases).The internal watershed infarction occurrencerate in the PMD group was higher than that in the non-PMD group (26.7 ％ vs.5.3％,p=0.001).The occurrence rate of penetrating arterial infarction (PAI) had no statistical difference between the PMD group and non-PMD group(42.2％ vs.35.5％,P=0.463).PAI was further divided into perforating branch atheromatous disease (BAD) and lipohyalinitic degeneration (LD).The occurrence rate of BAD in the PMD group was significantly higher than that in the non-PMD group (28.9％ vs.9.2％,P=0.005).The stepwise Logistic regression analysis indicated that watershed infarction [odds ratio (OR):9.750,95 ％ confidence interval(CI):2.828-33.612,P=0.000] and BAD lesion (OR:6.036,95 ％ CI:2.119-17.190,P =0.001) were the independent risk factors contributing to PMD.Conclusion Internal watershed infarction and BAD lesion may predict the PMD occurrence.The infarct location is conducive to find the high risk population of cerebral infarction progress.

OBJECTIVE To prepare Neuritic acid oral emulsion ，to optimize its formulation and preparation technology ，and to investigate its stability. METHODS Neuritic acid oral emulsion was prepared by mechanical method. On the basis of single factor experiment ，the appearance ，centrifugal stability ，centrifugal stability constant （Ke）and particle size of the emulsion as indexes，the formulation was optimized by orthogonal design ，taking the dosage of oleic acid ，octylphenol polyoxyethylene ether-10 and propylene glycol as factors ，the preparation technology was optimized by taking emulsification temperature ，shear time，pressure of high-pressure homogenization and cycle times of high-pressure homogenization as factors. The content of neuritic acid was determined by high performance liquid chromatography. The stability of Neuritic acid oral emulsion was investigated by high temperature test ，accelerated test and long-term test. RESULTS The optimal formulation and preparation technology were as follows：neuritic acid of 1 g，oleic acid of 5％ ，octylphenol polyoxyethylene ether- 10 of 4％ ，propylene glycol of 2％ ， emulsification temperature of 60 ℃ ，shear time of 2 min，homogenization pressure of 40 MPa and cycle times of twice. After three experiments ，the average particle size of Neuritic acid oral emulsion was 158.05 nm（RSD＝1.58％，n＝3），the average Ke was 0.39（RSD＝1.49％，n＝3），and the appearance was uniform milky white ，there was no stratification. The results of high temperature test showed that Neuritic acid oral emulsion was prone to stratification in high temperature environment ，and the content of neuritic acid increased. The results of accelerated test and long-term test showed that there was no significant change in the appearance or the content of neuritic acid when Neuritic acid oral emulsion was placed at room temperature for 6 months. CONCLUSIONS The formulation and preparation technology are stable and feasible ，and can be used for the preparation of Neuritic acid oral emulsion. Neuritic acid oral emulsion should not be placed in high temperature environment. It has good stability at room temperature for 6 months.