Aim: To investigate the chemical components in Zhizidahuang decoction( ZZDHD) to reveal the possible material therapeutic basis. Methods: High performance liquid chromatography coupled with photodiode array detector and tandem mass spectrometry ( HPLC-PDA-MS/MS) was applied to simultaneously characterize the chemical components and their structures in ZZDHD. The analysis was preformed on a Lichrospher C_(18) column using a binary eluent of 0. 1% acetic acid( A) and methanol( B) mixed under the gradient mode; UV spectra were scaned from 210 nm to 480 nm; negative ESI experiments in data-dependent scan mode were performed. Results: HPLC-PDA-MS/MS chromatogram of ZZDHD was achieved. Based on UV spectral data, information of molecular weight and mass fragmentation behaviors connected with extracted ion current( EIC) chromatogram, twenty kinds of components were detected and identified, including flavonoids, anthraquinones, iridoids and other constituents. Conclusion: The method presented in this study, which combined HPLC with UV and MS, allowes the characterization of compounds in the complex herbal system even without the reference standards.

Objective Analysis Chinese Journal Of Behavioral Medicine And Brain Science articles (CJBMBS) in 2009 year. Methods All the articles in CJBMBS which were published periodically on 12 issues in 2009 distribute were taken as the object, papers such as the Notices,Posters and Conferences were not included.Analysis the number of articles published, the article columns, the articles published style and the quotation quantity and so on. Results The CJBMBS in 2009 year altogether published 445 pieces papers, 12 issues, 1152 pages. Each issue carries articles 37.08 pieces equally and each article averages 2.59 pages. The density of each article is 0.3863,the information density is 6.181. The foundation investigative manuscripts account for 18.20%;the clinical investigative manuscripts account for 34.38%; the sanitary precaution manuscripts accounts for 28.09% ;the psychological behavior appraisal class manuscripts account for 6.07%;the monograph class manuscript accounts for 4.72; Summarizes 8.09%. The quotation quantity are 5252,each article' s quotation averages 11.80;The quotation quantity differs greatly, review articles' are the highest to be 34.67, while the short articles' are the lowest to be 10.43. The Pryse index mean value is 58.13%. The review articles' cited frequency is lowest to be 22.52% ,and that of short article report is highest to be 68.78%;the average self cited frequency is 17.86%.Conclusions CJBMBS in 2009 has plentiful information resources;among the total published articles papers are the chief.The quotation quantity per article is higher than average quotation quantity in scientific article in China. The Pryse index is higher than the mean value of various scientific and technology disciplines. Self cited frequency is moderate.

Objective To analyze the articles and authors published in Journal of Chinese Behavioral Medicine and Brain Science(JCBMBS) in 2012.Methods By using the bibliometric statistical methods,all papers published in JCBMBS in the whole year of 2012 were analyzed,including the paper amount,the paper column,the published style of papers,the institute distributions and the area distributions of the first authors,the foundation supported papers amount,the author amount,the citation amount and the published delay.Results 375 papers were published in JCBMBS in 2012,31.25 papers were published in an average issue,and one article included 3.06 pages averagely.The paper density was 0.327 and the information density was 5.21.The main style of published papers was original article,which accounted for 79.7％,and the least style was experts forum articles,which accounted for 2.4％.The amount of clinical research articles were 129,the hygienic articles were 89 and the basic research articles were 80.The area distributions of the first author were wide and imbalance.The top 6 provinces of the most published paper amounts in 2012 were Shandong,Jiangsu,Guangdong,Beijing,Anhui and Henan,where published paper amounts in total were 207 papers,and accounted for 55.2％ of the total papers of the whole year.There were 244 articles supported by various foundations and accounted for 65.06％ of the total papers of the whole year,in which 30.93％ was supported by the national foundations.The average author amount of the published papers,the average citation amount and the average published delay were 4.71 authors,15.58 indexes and 161.5 days respectively.12 corresponding authors had published 3 or more than 3 articles,and 37 corresponding authors had published 2 articles in the year.The top fifteen institutes of the most paper amounts in the periodical had published 145 articles,accounting for 38.7％ of the total amount of the papers.Conclusion The JCBMBS have an aboundant information resource;the main style of the published papers is original article;the clinical researches are the most in all columns.There are higher foundation supported papers and steady authors of this periodical.

Objective To analyze the papers published in Journal of Chinese Behavioral Medicine and Brain Science(JCBMBS) from 2009 to 2014 so as to know well of the characteristics of published papers and the progress of the journal.Methods Based on the published magazines,Wanfang database and the Magtech system,bibliometric analysis was conducted to analyze the quantity,types,authors,cooperation,citations and the funds of all the papers published from 2009 to 2014.Results A total of 2 343 papers were published from 2009 to 2014 with the average 390.50 items per year,32.54 items per tissue and 0.34 items per page.It was stable in columns setting and publishing types while the original papers accounted for more than 70％ every year.Zero fund-supported papers decreased year by year and the funds were 1.41-1.83 per article while the ratio of national funds supported papers were 20％-31％.It had a steady author group and the high produced institutions maintained stable.The ratio of cooperation in institutions were 45％-58％.The ratio cooperation in authors were 95.45％-97.09％ and it was high and steady.The citations were 12.45-18.48 per article which increased year by year.Conclusion From 2009 to 2014,the annual published articles on JCBMBS decrease,the paper density decreases and the citations increase year by year.It is relatively steady in columns setting,core authors,ratio of fund-supported papers and cooperation.

Objective To further investigate the relationship between apolipoprotein E(APOE) genotype and trait anxiety in patients with coronary heart disease (CHD).Methods Use the state-trait anxiety inventory (STAI) to investigate the state anxiety and trait anxiety of 107 CHD patients.According the score of TAI,the CHD patients were divided into CHD with high trait anxiety group ( n=39)and low trait anxiety group(n=68).The genotypes and alleles of apolipoprotein E gene was detected in all CHD patients and 50 healthy control subjects with the polymerase reaction(PCR) and restriction fragment length polymorphisms(RFLP) technique.Correlation analysis and logistic regression analysis were used.Results Apolipoprotein E gene showed positive relationship with trait anxiety score( OR =9.251,95％ CI2.726 ～ 18.266).F4 allele entered the regression equaltion Y =30.252 + 0.048X3 (P ＜ 0.05 ).Conclusion The results suggest the polymorphisms of Apolipoprotein E e4 allele may be associated with the trait anxiety symptom.

To analyze the mortality risk of stroke in China by literature search.The mortality rate of stroke (age standardized) in China has declined in recent years.The mortality rate of stroke which increases with age is higher in rural areas than in urban areas,and is higher in men than in women in China.There are obvious regional differences and seasonal differences in the mortality of stroke in China.Different subtypes of stroke have different death characteristics.Low or high temperatures can increase the risk of mortality of stroke.With the change of epidemiological characteristics,the risk factors of mortality of stroke in China are also changing.Through the epidemiological study of stroke death,the mortality risk of stroke can be researched effectively,the epidemiological characteristics of stroke can be mastered,and the direction of prevention and treatment of stroke can be understood.Individualized and multi factor intervention for unhealthy lifestyle,unhealthy behavior and metabolic cardiovascular risk factors can promote the establishment of stroke prevention and control strategy in China.

Objective To establish a stable and reliable model of brain death in swine, and to provide a more stable model for investigating pathomorphology in brain tissue and for studying transplantation immunology during brain death. Methods Base on the classic methodology of increasing epidural intracranial pressure, Codman intracranial pressure moni-toring probes were implanted in landrace pigs invasively. According to the relationship between ICP and MAP, brain death models were established by increasing intracranial pressure slowly and intermittently, with real-time monitoring of the intra-cranial pressure. Results Among twelve experimental landrace pigs, one died from anesthetic accident, while the rest elev-en were successfully established as brain death models. With effective respiratory and circulatory support, those brain death models can be maintained for (31.3 ± 4.7) h. Brain death model establishement is a stable and reliable process demonstrated by transcranial Doppler, EEG, ECG, mean arterial blood pressure and other monitoring methods. After brain death is con-firmed, animal models can be maintained for a long time. Conclusion Our methodology of inducing brain death model un-der ICP monitoring is stable and easy to be standardized. It can also provide a more stable model for studying brain tissue pathomorphology and transplantation immunology.

Objective To investigate the effect of early enteral nutrition.Methods 248 patients with esophageal and gastric carcinoma were randomly divided into two groups,and received enteral nutrition(EN)and parenteral nutrition(PN)each continuously for 6 days after operation.The body weight,blood routine test,liver function,and postoperative day 8 were compared with those before operation.Results The body weight,red blood cell count,and the levels of hemoglobin,serum albumin and transaminase decreased less in EN group than those in PN group(P＜0.01).The complication rates of anastomotic fistula,pulmonary infection,and delayedincision healing and average volume of pleural effusion were 0,13.8%,0,780ml in EN groups,while 3.2%,28.2%,7.2%,1842ml in PN group.Conclusion Early postoperative enteral nutirtion after esophageal carcinoma surgery can improve nutritional status and reduce complications in comparision with parenteral nutrition.

Objective To reproduce a stable animal model of brain death in pigs, observe the change regularity of cerebral oxygen metabolism during the process of brain death, and to evaluate the significance and value of cerebral oxygen metabolism parameters for the diagnosis of brain death. Methods Twelve landrace pigs were used to create the brain death models using modified method of increasing epidural intracranial pressure (ICP). The mean arterial pressure (MAP) and ICP were monitored continuously during the process. The pigs were divided into four groups according to cerebral perfusion pressure (CPP) decreasing degree during brain death, namely CPP normal group and CPP decreasing 0%-30%, 30%-70%, and 70%-100% groups. Blood gas analysis of the external carotid artery and internal jugular vein were monitored discontinuously. The changes in cerebral oxygen metabolism parameters, including external carotid artery-internal jugular vein bulb oxygen content difference (AJDO2), internal jugular bulb-external carotid artery carbon dioxide partial pressure difference (DPCO2) and DPCO2/AJDO2 ratio, were observed. Results Brain death model were successfully reproduced in 12 experimental pigs. With MAP and ICP monitoring, the models at different stages of CPP could be repeatedly induced. The levels of AJDO2 and DPCO2 were increased gradually and then decreased, while the ratio of DPCO2/AJDO2 was constantly increased with the decrease of CPP. The level of AJDO2 in CPP decreasing 0%-30%group was significantly higher than that in CPP normal group [(5.86±1.21)% vs. (3.92±0.64)%], the levels of DPCO2 in CPP decreasing 0%-30% and CPP decreasing 30%-70% groups were significantly higher than those in CPP normal group [mmHg (1 mmHg = 0.133 kPa): 10.33±1.83, 11.48±2.32 vs. 6.11±1.43], and the ratios of DPCO2/AJDO2 in CPP decreasing 30%-70% and CPP decreasing 70%-100% groups were significantly higher than those in CPP normal group and CPP decreasing 0%-30% group (2.81±0.53, 4.12±1.07 vs. 1.57±0.64, 1.62±0.81). All the differences above were statistically significant (all P < 0.05). Conclusions With the decrease of CPP, cerebral oxygen metabolism showed a regular change during brain death. DPCO2 combined with DPCO2/AJDO2 is a reliable blood gas analysis index indicating intracranial hypoperfusion, which has certain reference value for the determination of brain death.

Objective To explore humor experience and its relationship with theory of mind in patients with schizophrenia. Methods sixty?one schizophrenic patients without treatment and sixty?three healthy subjects were assessed with the Humor Picture Test ( HPT) ,Humor Video Test ( HVT) and the Theory?of?Mind Picture?Se?quencing Task ( ToM?PST) . Results Compared with healthy subject in HPT,schizophrenic patients showed sig?nificantly higher non?humor picture rating score (24.41 ± 8.82 vs 28.41 ± 11.56, P<0.05),and less humor picture rating score (45.65 ± 11.49 vs 34.41 ± 13.06, P<0.05). Compared with healthy subject in HVT,schizophrenic patients also showed significantly less humor video rating score (3.56 ± 0.57 vs 2.46 ± 0.79, P<0.01) and the number of standard humorous moments (3.68 ± 1.19 vs 2.42 ± 1.40, P<0.01) and the sensitivity of standard hu?morous moments?d’(humor) score (1.11 ± 0.46 vs 0.47 ± 0.42, P<0.01). Schizophrenic patients had significant?ly less total score (39.89 ± 12.33 vs 58.76 ± 0.64, P<0.01) of ToM?PST compared to normal control. Correlation analysis showed a negative correlation between d’ ( humor) score and the Positive and Negative Syndrome Scale ( PANSS) total score ( r=-0.380, P<0.01) for the patients. There was significantly negative correlation between non?humor picture rating score and ToM?PST total score ( r=-0.316, P<0.05) ,and positive correlation between d’ (humor) score and ToM?PST total score ( r=0.400, P<0.01) in schizophrenic patient. After controlling for the PANSS total score,the correlation between d’(humor) score and ToM?PST total score remained significant. Conclusion Schizophrenic patients have poor humor experience,which is related to the impairments of theory of mind.Humor experience deficit may share a common neuropsychological base with the impairment of theory of mind.